ClBBM and ClPLT2 function redundantly during both male and female gametophytes development in watermelon

As a prerequisite for sexual reproduction,gametophyte development is an interesting process involving cell proliferation,differentiation and specialization.Gametogenesis has been extensively explored in model plants,but the regulatory mechanism of gametophyte development largely remains unknown in Cucurbitaceae species.In present study,we have shown that watermelon ClBBM and ClPLT2,two AP2/ERF transcription factors,participated in both male and female gametophyte development.Clbbm and Clplt2 single mutants resembled wild-type phenotypes in both vegetative and reproductive development.But Clbbm/Clplt2 double mutant showed partial pollen abortion and bore less seeds comparing to WT.Our results indicated that the abnormal pollen grains were caused by premature tapetum degeneration,and reduced seed-set was due to faulty embryo sac development.ClBBM and ClPLT2 were expressed in FG4 embryo sacs,and their transcripts were also detectable in the tapetum and microspore of stage 9 anthers,which was consistent with developmental stages of defective phenotypes observed in double mutant.The expression of genes essential for tapetum development,ClATM1,ClAMS,ClMS1 and ClMS188,was decreased in Clbbm/Clplt2 double mutants.Moreover,the transcriptome analysis indicated that ClBBM and ClPLT2 participated in tapetum and pollen wall development by regulate cell cycle,transmembrane transport,glucan and cellulose metabolic process.Collectively,ClBBM and ClPLT2 were functionally redundant in regulating gametophyte development in watermelon,and their functions differ from their homologous genes in model plant Arabidopsis.

The pseudo-type response regulator gene Clsc regulates rind stripe coloration in watermelon

The color and pattern of watermelon rind are crucial external traits that directly affect consumer preferences.Watermelons with stripes having a stronger color than the background rind are ideal for studying stripe patterns in plants,while there is still limited knowledge about the genetic mechanisms underlying stripe coloration due to the lack of germplasm resources.In this study,we focused on a watermelon germplasm with colorless stripes,and genetic analysis revealed that the trait is controlled by a single recessive gene.The gene Clsc(Citrullus lanatus stripe coloration),which is responsible for the colorless stripe,was localized into a 147.6 kb region on Chr9 by linkage analysis in a large F2 mapping population.Further analysis revealed that the Cla97C09G175170 gene encodes the APRR2 transcription factor,plays a crucial role in determining the watermelon colorless stripe phenotype and was deduced to be related to chlorophyll synthesis and chloroplast development.Physiological experiments indicated that Cla97C09G175170 may significantly influence chloroplast development and chlorophyll synthesis in watermelon.The results of this study provide a better understanding of the molecular mechanism of stripe coloration in watermelon and can be useful in the development of marker-assisted selection(MAS)for new watermelon cultivars.

Response of Watermelon to Gravel-Mulch and Supplementary Irrigation: Yield, Water Use Efficiency and Root Distribution

A field experiment was conducted to investigate the effect of supplementary irrigation on watermelon (Citullus lanatus) yield, water-use efficiency (WUE) and root distribution in gravel-mulched field in northwest Loess Plateau, China, during 2001 and 2002 growing seasons. The results showed that gravel mulch significantly improved seedling emergence, increased yield and WUE, and alleviated the influence of drought on plant growth. Regardless of gravel mulch application, supplementary irrigation increased watermelon yields, average fruit weight and number of fruit, especially yield increased as the amount of irrigation increased (P<0.05). Generally, WUE of irrigated treatments were higher than that of non-irrigation treatment in gravel-mulched field. The effect of water supply on root distribution was different in two years. In 2001, average root length density (RLD) and root weight density (RWD) whole the soil profile increased. In 2002, however, RLD and RWD decreased as water supply increased. The average RLD and RWD in 2001 were significantly higher than those in 2002. Maybe we can interpret the phenomenon with the theory that there is a need to optimize root distribution (in terms of water relations) and aboveground biomass for a given water supply.The yield may not depend as much on root growth as on the amount of water required at critical stages. A significant effect of soil depth on RLD and RWD were observed in both years, but did not rapidly decrease with depth.

Identification of an Aux/IAA regulator for flesh firmness using combined GWAS and bulked segregant RNA-Seq analysis in watermelon

Watermelon is a highly cultivated fruit crop renowned for its quality properties of fruit flesh.Among various quality factors,fruit flesh firmness is a crucial quality parameter influencing the fruit texture,shelf life and its commercial value.The auxin/indole-3-acetic acid(Aux/IAA)plays a significant role in fruit development and ripening of non-climacteric fruits.However,the regulatory mechanism of Aux/IAA in controlling fruit flesh firmness and ripening in watermelon remains unknown.In this study,we employed an integrative approach combining genome-wide association study(GWAS)and bulked segregant RNA-Seq analysis(BSR-Seq)to identify an overlapping candidate region between 12776310 and 12968331 bp on chromosome 6,underlying an auxin-responsive gene(Aux/IAA)associated with flesh firmness in watermelon.Transcriptome analysis,followed by real-time quantitative reverse transcription PCR(qRT-PCR),confirmed that the expression of Aux/IAA was consistently higher in fruits with high flesh firmness.The sequence alignment revealed a single base mutation in the coding region of Aux/IAA.Furthermore,the concomitant Kompetitive/Competitive allele-specific PCR(KASP)genotyping data sets for F2 population and germplasm accessions identified Aux/IAA as a strong candidate gene associated with flesh firmness.Aux/IAA was enriched in the plant hormone signal transduction pathway,involving cell enlargement and leading to low flesh firmness.We determined the higher accumulation of abscisic acid(ABA)in fruits with low flesh firmness than hard flesh.Moreover,overexpression of Aux/IAA induced higher flesh firmness with an increased number of fruit flesh cells while reducing ABA content and flesh cell sizes.Additionally,the allelic variation in Aux/IAA for soft flesh firmness was found to exist in Citrullus mucosospermus and gradually fixed into Citrullus lanatus during domestication,indicating that soft flesh firmness was a domesticated trait.These findings significantly enhanced our understanding of watermelon fruit flesh firmness and consequently the watermelon fruit quality.

Information Technology Research on Productivity Evaluation of Gravel-mulched Field in Ningxia

The evaluation process of traditional land productivity is relatively complex,and the evaluation indices and results cannot display visually.With the development of geographic information system science,there are more and more studies on the GIS-based land productivity evaluation.GIS system can combine the evaluation model with information system software,realizing the visual display of all the evaluation factors.In this paper,with the development process of GIS-based management information system as references,the management information system for GIS-based gravel-mulched field productivity evaluation was designed and developed by combining with the SI model for gravel-mulched field productivity evaluation.The system was developed under VB environment,integrated with MapInfo,and the evaluation results could be shown visually in the system windows.

Enhancer of Shoot Regeneration 2(ESR2)regulates pollen maturation and vitality in watermelon(Citrullus lanatus)

Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male sterile materials and facilitating cross-breeding.Despite its importance,there is a lack of research on the regulation mechanism of male reproductive development in watermelon.In this study,we identified that ClESR2,a VIIIb subclass member in the APETALA2/Ethylene Responsive Factor(AP2/ERF)superfamily,was a key factor in pollen development.RNA insitu hybridization confirmed significantClESR2 expression in the tapetum and pollen during the later stage of anther development.The pollens of transgenic plants showed major defects in morphology and vitality at the late development stage.The RNA-seq and protein interaction assay confirmed that ClESR2 regulates pollen morphology and fertility by interacting with key genes involved in pollen development at both transcriptional and protein levels.These suggest that Enhancer of Shoot Regeneration 2(ESR2)plays an important role in pollen maturation and vitality.This study helps understand the male reproductive development of watermelon,providing a theoretical foundation for developing male sterile materials.

Additional far-red light promotes adventitious rooting of doubleroot-cutting grafted watermelon seedlings

Root regeneration is an important factor influencing the healing rate of graft union and the survival of double-root-cutting grafting.To date,little information is available on how to enhance root regeneration of rootstock in grafted watermelon(Citrullus lanatus)seedlings.In this study,the effects of different light treatments on root regeneration were determined.This revealed that addition of far-red light(Fr)could significantly expedite root formation in the rootstock.Moreover,the results of transcriptome analysis revealed that plant hormone pathway and auxinrelated genes were greatly induced by Fr,especially for auxin-response proteins(including CmIAA11,CmIAA17,and CmAUX28),Small auxinup RNA genes(including CmSAUR20 and CmSAUR50)and the auxin efflux transporter(CmPIN3).In addition,the expression of Phytochrome Interacting Factor(PIFs),such as CmPIF1,CmPIF3 and CmPIF7,was remarkably increased by Fr.These genes may act together to activate auxinrelated pathways under Fr treatment.Based on the results of HPLC-MS/MS analysis,the concentrations of different auxin-types in adventitious root were significantly influenced by Fr.Furthermore,the better growth of rootstock root displayed superior vasculature transport activity of the graft union with Fr treatment,which was determined by the acid magenta dyeing experiment.Therefore,all the results suggested that Fr could induce AR formation in rootstocks,which may be associated with the auxin accumulation by regulating the transcriptional level of auxinrelated and PIF genes.The findings of this study demonstrated a practicable way to shorten the healing period of graftings and improve the quality of grafted watermelon seedlings,which will provide a theoretical basis for the speeding development of industrialized seedlings production.

Quantitative trait loci identification reveals zinc finger protein CONSTANS-LIKE 4 as the key candidate gene of stigma color in watermelon(Citrullus lanatus)

Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow stigma.Bulk segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma color.Also,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a~1.01 Mb interval that harbors 51 annotated genes.Cla97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon panel.The expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing data.Stigma color formation is often related to carotenoids,and we also found that the expression trend of ClCHYB(annotated asβ-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma accession.These data indicated that Cla97C06G117020 and ClCHYB may interact to form the stigma color.This study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon.

Effects of Functional Organic Materials on Morphological Indexes,Quality and Yield of Watermelon

[Objectives]This study was conducted to explore suitable organic compound application models for watermelon growth.[Methods]With watermelon hybrid material"M22×P18"as the test material,the effects of four functional organic materials,namely garlic straw treatment(T_(1)),onion straw treatment(T_(2)),garlic straw+sheep manure treatment(T_(3))and onion straw+chicken manure treatment(T_(4)),on the morphological indexes,yield and quality of watermelon were investigated.[Results]Different functional organic materials had different effects on morphological indexes,yield and quality of watermelon.The morphological indexes,nutritional quality indexes and yield of watermelon treated with garlic straw and sheep manure compound(T_(3))and onion straw and chicken manure compound(T_(4))were significantly higher than those treated simply with garlic straw(T_(1))and onion straw(T_(2)),and T_(3)performed relatively better.Compared with treatment T_(2),T_(3)showed a stem diameter,vine length and leaf number increasing by 43.05%,46.69%and 40.77%respectively,central sugar and side sugar contents increasing by 11.72%and 21.90%respectively,and a yield increasing by 42.91%,with significant differences from T_(2).[Conclusions]This study provides technical support for high-quality and high-yielding cultivation of watermelon.

Effects of Functional Organic Materials on Fresh Weight,Quality,Single-melon Weight and SPAD Value of Watermelon

[Objectives]This study was conducted to explore a functional organic material formula suitable for watermelon cultivation with high quality,high yield and high efficiency.[Methods]Four treatments were set in the experiment,namely four functional organic materials,garlic straw treatment(T_(1)),onion straw treatment(T_(2)),garlic straw+sheep manure treatment(T_(3))and onion straw+chicken manure treatment(T_(4)),to investigate the effects of different functional organic materials on fresh weight,quality,single-melon weight and SPAD value of watermelon.[Results]The effects of different functional organic materials on fresh weight,quality,single-melon weight and SPAD value of watermelon were quite different.The fresh weight,quality,single-melon weight and SPAD value of watermelon were higher in treatment T_(3)applying garlic straw and sheep manure and treatment T_(4)applying onion straw and chicken manure than in treatment T_(1)applying garlic straw and treatment T_(2)applying onion straw.Specifically,the fresh weight of whole plant was the highest in treatment T_(3),followed by treatment T_(4),and the values of the two treatments increased by 12.83%and 5.94%respectively compared with treatment T_(1);the weight of single melon was the highest in treatment T_(3),followed by treatment T_(4),and the values of the two treatments increased by 42.45%and 31.77%respectively compared with treatment T_(2);and the SPAD values of treatments T_(3)and T_(4)were significantly higher than those of treatments T_(1)and T_(2),and the value of treatment T_(3)was the largest.[Conclusions]This study provides theoretical support for the popularization and application of fertilization techniques combining organic fertilizers and reduced chemical fertilizers for watermelon.

小果型西瓜新品种“炫美1号”的选育

“炫美1号”是以‘HM101’为母本、‘FN104’为父本杂交选育而成的F_(1)代小型西瓜新品种。华北地区春季大棚种植,果实发育期28~30 d,全生育期88~90 d。植株生长势中等,坐果性好。果实近圆形,果皮底色绿,上覆墨绿色条纹,果皮厚度0.5 cm。平均单果质量1.9 kg,果皮韧,肉质脆,果肉红黄双色,中心可溶性固形物含量13.1%,边缘可溶性固形物含量10.8%。667 m^(2)产量达3100 kg以上,适合华北地区设施栽培,2023年9月通过农业农村部非主要农作物品种登记。

西瓜专用瓠瓜砧木苏砧1号种子纯度的KASP鉴定

为准确、高效地鉴定西瓜专用瓠瓜砧木苏砧1号杂交种子纯度,以苏砧1号及其亲本为试验材料,结合简化重测序和KASP技术,筛选并获得2个稳定多态性KASP标记(SNP1101和SNP1102)。利用2对KASP标记进行苏砧1号的537株单株基因型检测,并结合田间表型验证标记准确性。结果表明,该批苏砧1号葫芦类型砧木种子纯度为99.44%,2对KASP标记鉴定结果与田间表型鉴定结果一致;并且可将苏砧1号与其他葫芦类型砧木品种区分开。以上结果可为高效、准确地鉴定瓠瓜类型砧木品种及种子纯度提供便捷的方法。

不同整枝和留叶数量对嫁接西瓜光合特性及产量品质的影响

以嫁接西瓜为试材,采用基质槽培方法,研究不同整枝及留叶数量对嫁接西瓜光合特性、植株干物质积累、根系形态及产量品质的影响,以期为嫁接西瓜产量品质提升提供参考依据。结果表明:不同侧蔓保留叶片数量显著影响主蔓叶片光合速率和植株物质积累,伴随叶片总数从25片递增到45片,主蔓的光合速率、茎叶干物质和果实可溶性固形物含量也显著增加,在40片叶处理下,根系干物质和产量增加至最高后下降。相关分析表明根系形态指标与果实中可溶性糖含量呈显著正相关,40片叶处理根系干物质量、根长、根表面积和体积均高于其他处理,尤其是在0~0.5 mm径级范围内的根长、根尖数和根尖长度,0.5~2.0 mm径级范围内的投影面积和表面积高于其他处理。因此生产中推荐嫁接小型西瓜保留2条侧蔓,分别保留10片和5片叶去顶,可增加西瓜产量,提高品质。

西瓜NPR基因家族的鉴定及其在盐胁迫下的表达分析

以拟南芥NPR基因家族成员蛋白序列为查询序列,在西瓜基因组数据库中鉴定西瓜NPR基因家族成员,并对其进行生物信息学分析及盐胁迫下的表达分析。共鉴定到4个成员,将其命名为ClNPR1~ClNPR4,其编码蛋白的理化性质、保守功能结构域、重要氨基酸残基及motif分析结果与其他物种有较高的一致性,顺式作用元件分析显示,ClNPRs与非生物胁迫相关。300 mmol·L^(-1)NaCl处理耐盐材料和盐敏感材料,分别取盐胁迫0、8、24 h的叶片进行4个NPR基因的表达模式分析,结果表明,ClNPR1在盐敏感和耐盐材料中都显著上调表达,但盐胁迫24 h,ClNPR1在盐敏感材料中显著下调表达,在耐盐材料中虽然下调但还保持较高的表达水平,且在耐盐材料中的表达量显著高于盐敏感材料;盐胁迫24 h,ClNPR2和ClNPR3在盐敏感和耐盐材料中都显著上调表达,在耐盐材料中的表达量均显著高于盐敏感材料。推测ClNPRs基因在西瓜响应盐胁迫的过程中扮演重要角色。

Screening of Soil Antagonistic Bacteria for Watermelon Fusarium Wilt

[Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applying the antagonistic bacteria strains into soil.[Method] Actinomycete strains,fluorescent bacteria strains and bacillus strains were isolated from soil samples by the dilution-plate method,then its resistance was screened respectively by the improved confront culture method after colonies were purified.Finally,bacteria strains with better antagonistic effect were identified.[Result] 29 bacteria strains with stable antagonistic effect against watermelon fusarium wilt were screened from 39 soil samples,which contained 15 fluorescent bacteria strains,5 bacillus strains and 9 actinomycete strains.Furthermore,three antagonistic bacteria strains of FM2,FM3 and FM4 with the strongest antagonism were identified primarily.[Conclusion] According to cultural characteristics,morphological observation,biochemical and physiological tests,FM2 belongs to bacillus subtilis,while FM3 and FM4 belong to micrococcus.

AFLP Fingerprint and SCAR Marker of Watermelon Core Collection

The identification of germplasm is an important step for effective utilization of the available germplasm. In previous studies, isoenzyme, RAPD and SSR techniques had been used to conduct the genetic identification of watermelon ( Citrullus lanatus (Thunb.) Mansf.), but their effectiveness was limited due to the extremely narrow genetic background among watermelon genotypes. In this research, amplified fragment length polymorphism (AFLP), which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct genetic analysis and variety identification of thirty genotypes of watermelon core collection that represent a wide range of breeding and commercially available germplasm. As a result, a DNA fingerprint based on 15 bands amplified with four primer combinations was developed. In this fingerprint, each genotype has its unique fingerprint pattern and can be distinguished from each other. Furthermore, in or der to facilitate the utilization of AFLP marker in practice, one specific AFLP band of genotype 'PI296341' coming from fragment amplified by primer combination E-AT/M-CAT was successfully converted into a sequence characterized amplified region (SCAR) marker.

Benefit of Interplanting Sugarcane with Watermelon and Soybean

This study was conducted to investigate the effects of interplanting of sugarcane with watermelon and soybean on agronomic traits, yields, quality and benefit of crops, so as to provide reference for extension of the interplanting mode of sugarcane with watermelon or soybean. There were 4 treatments： CK1 （sole planting of sugarcane, no fertilization）, CK2 （sole planting of sugarcane, applying chemical fertilizers）, T1 （interplanting sugarcane with watermelon, applying chemical fertilizers ＋ cow dung）, and T2 （interplanting sugarcane with soybean, applying chemical fertilizers ＋ cow dung）. The results showed that compared with CK2, the sugarcane yield of treatment T1 reached 2.5 t/hm2, the watermelon yield was 1.02 t/hm2, and the total economic benefit increased by 3090 Yuan/hm2; the sugarcane yield in treatment T2 decreased by 7.61 t/hm2 （but not significant）, and the soybean yield was 0.35 t/hm2, and the total economic benefit increased by 720 Yuan/hm2; and the total sugar content in watermelon flesh was 8.59%, and the crude protein content in soybean was 36.15%, satisfying the quality indexes of the same agricultural products. Compared with sole planting of sugarcane, sugarcane plus watermelon or soybean interplanting mode improved economic benefit, treatment T1 showed economic benefit far higher than treatment T2, and is suitable for sugarcane regions with good water and fertilizer conditions and enough labor, and the T2 mode has simple operation, and is suitable for large-area extension.

Mixed Substrate Soilless Cultivation Techniques for Mini Watermelon in Spring Greenhouse of Beijing Area

Watermelon, as a traditional dominant crop in Beijing, has obvious indus- trial advantages. The mixed substrate soilless cultivation techniques developed by the Beijing Agricultural Technology Extension Station for mini watermelon in spring greenhouse can effectively solve the problem of continuous cropping in facility wa- termelon, and they are conducive to green, safe and efficient development of water- melon industry for fixed nutrient solution formula, automatic water and fertilizer irri- gation, stable product quality and facilitating large-scale and standardized production.

Spatial Characteristic Analysis of the Comparative Advantage of Watermelon and Melon Production in Hainan Province

[Objective] This study investigated the spatial characteristics of counties （cities） with comparative advantages in watermelon and melon production to provide reference bases in formulating strategies for the development of watermelon and melon industries in Hainan Province. [Method] By using the sowing area, total yield, and yield per unit area of watermelon and melon in Hainan Province as research u- nits, the yield comparative advantage （YCA）, efficiency comparative advantage （E- CA）, scale comparative advantage （SCA）, concentration ratio comparative advantage （CRCA）, comprehensive comparative advantage （CCA）, ratio of yield per unit area （RYPA）, sowing area ratio （SAR）, and distribution characteristics of watermelon and melon were systematically analyzed. By referring to the agricultural statistic data of 18 counties （cities） in Hainan Province, indexes for each research unit （i.e., the YCA index, ECA index, SCA index, CRCA index, CCA index, RYPA index, and SAR index） were established and calculated to determine the comparative advantage of watermelon and melon production in Hainan Province. A spatial expression of the research result on a map was conducted by using GIS software. [Result] Seven counties （cities） exhibited comparative advantages in watermelon production, namely, Lingshui, Wanning, Wenchang, Dongfang, Sanya, Ledong, and Changjiang. The Eastern and Southern Hainan Provinces had CCAs, and the Western and Northern Hainan Provinces could be reserved for future development. For melon production, four counties （cities） exhibited comparative advantages, namely, Ledong, Lingshui, Sanya, and Dongfang. The Southern Hainan Province had CCA, whereas the West- ern Hainan Province could be reserved for later development. [Conclusion] The result has showed that establishing watermelon and melon as dominant agricultural prod- ucts is necessary for the future development of the industry and for the formulation of a layout of regions with advantages, where key support and construction should be provided preferentially with the aim to raise the yield, quality, and market com- petitiveness of products.

Effects of Exogenous Calcium(Ca) on the Growth of Watermelon Seedlings under NaCl Stress

[Objective] This study was conducted to reveal the effect of exogenous calcium （Ca） in mitigating the damage caused by NaCI stress to watermelon seedlings. [Method] To the liquid medium containing 100 mmol/L NaCI, CaSO4 was added to the final concentrations of 4, 6, 10 and 14 mmol/L. The morphological and physiological indices including plant height, stem diameter, number of leaves per plant, maximum leaf length, maximum leaf width, seedling fresh weight and dry weight, malondialdehyde （MDA） content, relative permeability of plasma membrane, ascorbic acid （AsA） content, free proline content, soluble sugar content and soluble protein content in leaves of the seedlings treated with above media for 10 d were measured. [Result] NaCI stress significantly inhibited the growth of watermelon seedlings. The membrane permeability and MDA content in leaves were significantly increased under NaCI stress, and the contents of proline, soluble sugar, soluble protein and AsA were increased to different extents. Exogenous CaSO4 influenced the morphological and physiological indices of seedlings under NaCI stress in a manner. Treated with 6 mmol/L CaSO4, the growth of seedlings under salinity was significantly improved; membrane permeability and MDA content were drastically reduced, the contents of proline, soluble sugar, soluble pro- tein and AsA in leaves were significantly increased. [Conclusion] Exogenous CaSO＋ alleviates lipid peroxidation and inhibition of seedling growth under NaCI stress by improving the abilities of antioxidation and osmotic adjustment, and 6 mmol/L CaSO4 showed the best effect.