Elizabeth’s arrival in our life came by a chance event.But she makes me believe that in America,in China and in any other corner of the world,there are people who have lofty minds and who are ready to give their love...Elizabeth’s arrival in our life came by a chance event.But she makes me believe that in America,in China and in any other corner of the world,there are people who have lofty minds and who are ready to give their love to others.I believe this is especially true among women,as they communicate with not only language but also with their heart.It is because of women that this world has a better understanding among peoples of all nationalities.It makes this world a better place to live in.展开更多
Grass carp reovirus(GCRV),a disaster agent to aquatic animals,belongs to Genus Aquareovirus of family Reoviridea.Sequence analysis revealed GCRV genome segment 8(s8) was 1 296 bp nucleotides in length encoding an inne...Grass carp reovirus(GCRV),a disaster agent to aquatic animals,belongs to Genus Aquareovirus of family Reoviridea.Sequence analysis revealed GCRV genome segment 8(s8) was 1 296 bp nucleotides in length encoding an inner capsid protein VP6 of about 43kDa.To obtain in vitro non-fusion expression of a GCRV VP6 protein containing a molecular of fluorescence reporter,the recombinant baculovirus,which contained the GCRVs8 and eGFP(enhanced green fluorescence protein) genes,was constructed by using the Bac-to-Bac insect expression system.In this study,the whole GCRVs8 and eGFP genes,amplified by PCR,were constructed into a pFastBacDual vector under polyhedron(PH) and p10 promoters,respectively.The constructed dual recombinant plasmid(pFbDGCRVs8/eGFP) was transformed into DH10Bac cells to obtain recombinant Bacmid(AcGCRVs8/eGFP) by transposition.Finally,the recombinant bacluovirus(vAcGCRVs8/eGFP) was obtained from transfected Sf9 insect cells.The green fluorescence that was expressed by transfected Sf9 cells was initially observed 3 days post transfection,and gradually enhanced and extended around 5 days culture in P1(Passage1) stock.The stable high level expression of recombinant protein was observed in P2 and subsequent passage budding virus(BV) stock.Additionally,PCR amplification from P1 and amplified P2 BV stock further confirmed the validity of the dual-recombinant baculovirus.Our results provide a foundation for expression and assembly of the GCRV structural protein in vitro.展开更多
As one of the important parts of urban ecosystem, urban green space plays an irreplaceable role in maintaining the balance of carbon and oxygen, absorbing the pollution and removing dust, killing bacteria, purifying s...As one of the important parts of urban ecosystem, urban green space plays an irreplaceable role in maintaining the balance of carbon and oxygen, absorbing the pollution and removing dust, killing bacteria, purifying soil and water. To analyze the spatio-temporal changes of humidifying effect of green lands at the spring daytime, the authors monitored the air humidity within 1.8 m high from various underlying surfaces, including arbor-grass lands, shrub lands, herb lands and a cement pavement, from late February to late April in 2015 and 2016, when it was sunny and windless, in the Yuxi park of Shijiazhuang. The results were compared with the monitoring data of a bare land(190 m×120 m) without environmental interventions such as vegetation and water. The results could be summarized as follows:(1) The humidifying effect of green spaces was the weakest at 8:00 a.m. during the late February, whereas it was the strongest at 10:00 a.m.; from late March to late April, the humidifying effect of green spaces strengthened with the increase of air temperature, and the strongest was noted around 12:00-16:00.(2) The humidifying effect of various types of green lands was the strongest close to the ground, and reduced from 0.2 m to 1.8 m. This vertical stratification became apparent after the plants sprouted, the vertical difference of humidifying effect was the most significant in the herb lands, less significant in arbor-grass lands, and least in the shrub lands.(3) Multiple comparison analysis of the humidity data showed that relative humidity of all green lands achieved the significant level(P < 0.05), indicating the strong effect of landscape plants in adjusting atmospheric humidity in spring.(4) The humidifying effect of the artificial lake was more remarkable before the plants sprouted, however, with the rapid growth of plant leaves, theeffect gradually reduced and became even weaker than the effect of vegetation. Although the humidifying effect in spring was weaker than that in summer and autumn, the effect of humidification was more complicated during the growth of green leaves in spring than in summer and autumn. This is useful to construct the theory system of ecological environment effect of green lands in spring, summer and autumn.展开更多
由深路堑土石方开挖而形成的高陡光滑岩质边坡往往具有很大安全隐患和环境影响,需要采用有效的绿化防护技术以增强其稳定性和发挥生态功能。结合某高速公路扩建工程施工,从土工材料与植物生态条的结合、植物品种的选择与组合、以及土基...由深路堑土石方开挖而形成的高陡光滑岩质边坡往往具有很大安全隐患和环境影响,需要采用有效的绿化防护技术以增强其稳定性和发挥生态功能。结合某高速公路扩建工程施工,从土工材料与植物生态条的结合、植物品种的选择与组合、以及土基加固等方面,探讨了适用于陡峭且光滑岩质边坡的绿化防护关键技术,包括TBS(turf base seeding)植草(灌)防护和喷播养护策略,增加客土厚度和采用双层TBS镀锌网片加固等。实践表明,上述措施能显著提升植被的绿化成活率和边坡的稳定性。研究结果可为类似地质环境下的绿化防护工程的设计与施工提供有价值的参考。展开更多
文摘Elizabeth’s arrival in our life came by a chance event.But she makes me believe that in America,in China and in any other corner of the world,there are people who have lofty minds and who are ready to give their love to others.I believe this is especially true among women,as they communicate with not only language but also with their heart.It is because of women that this world has a better understanding among peoples of all nationalities.It makes this world a better place to live in.
基金National Natural Science Foundation of China (Grant Nos 30470074,30671615)Innovation Project of the Chinese Academy of Sciences (KSCX2-YW-N-021).
文摘Grass carp reovirus(GCRV),a disaster agent to aquatic animals,belongs to Genus Aquareovirus of family Reoviridea.Sequence analysis revealed GCRV genome segment 8(s8) was 1 296 bp nucleotides in length encoding an inner capsid protein VP6 of about 43kDa.To obtain in vitro non-fusion expression of a GCRV VP6 protein containing a molecular of fluorescence reporter,the recombinant baculovirus,which contained the GCRVs8 and eGFP(enhanced green fluorescence protein) genes,was constructed by using the Bac-to-Bac insect expression system.In this study,the whole GCRVs8 and eGFP genes,amplified by PCR,were constructed into a pFastBacDual vector under polyhedron(PH) and p10 promoters,respectively.The constructed dual recombinant plasmid(pFbDGCRVs8/eGFP) was transformed into DH10Bac cells to obtain recombinant Bacmid(AcGCRVs8/eGFP) by transposition.Finally,the recombinant bacluovirus(vAcGCRVs8/eGFP) was obtained from transfected Sf9 insect cells.The green fluorescence that was expressed by transfected Sf9 cells was initially observed 3 days post transfection,and gradually enhanced and extended around 5 days culture in P1(Passage1) stock.The stable high level expression of recombinant protein was observed in P2 and subsequent passage budding virus(BV) stock.Additionally,PCR amplification from P1 and amplified P2 BV stock further confirmed the validity of the dual-recombinant baculovirus.Our results provide a foundation for expression and assembly of the GCRV structural protein in vitro.
基金Supported by the National Science and Technology Major Project(2011ZX05043-005)
文摘As one of the important parts of urban ecosystem, urban green space plays an irreplaceable role in maintaining the balance of carbon and oxygen, absorbing the pollution and removing dust, killing bacteria, purifying soil and water. To analyze the spatio-temporal changes of humidifying effect of green lands at the spring daytime, the authors monitored the air humidity within 1.8 m high from various underlying surfaces, including arbor-grass lands, shrub lands, herb lands and a cement pavement, from late February to late April in 2015 and 2016, when it was sunny and windless, in the Yuxi park of Shijiazhuang. The results were compared with the monitoring data of a bare land(190 m×120 m) without environmental interventions such as vegetation and water. The results could be summarized as follows:(1) The humidifying effect of green spaces was the weakest at 8:00 a.m. during the late February, whereas it was the strongest at 10:00 a.m.; from late March to late April, the humidifying effect of green spaces strengthened with the increase of air temperature, and the strongest was noted around 12:00-16:00.(2) The humidifying effect of various types of green lands was the strongest close to the ground, and reduced from 0.2 m to 1.8 m. This vertical stratification became apparent after the plants sprouted, the vertical difference of humidifying effect was the most significant in the herb lands, less significant in arbor-grass lands, and least in the shrub lands.(3) Multiple comparison analysis of the humidity data showed that relative humidity of all green lands achieved the significant level(P < 0.05), indicating the strong effect of landscape plants in adjusting atmospheric humidity in spring.(4) The humidifying effect of the artificial lake was more remarkable before the plants sprouted, however, with the rapid growth of plant leaves, theeffect gradually reduced and became even weaker than the effect of vegetation. Although the humidifying effect in spring was weaker than that in summer and autumn, the effect of humidification was more complicated during the growth of green leaves in spring than in summer and autumn. This is useful to construct the theory system of ecological environment effect of green lands in spring, summer and autumn.
文摘由深路堑土石方开挖而形成的高陡光滑岩质边坡往往具有很大安全隐患和环境影响,需要采用有效的绿化防护技术以增强其稳定性和发挥生态功能。结合某高速公路扩建工程施工,从土工材料与植物生态条的结合、植物品种的选择与组合、以及土基加固等方面,探讨了适用于陡峭且光滑岩质边坡的绿化防护关键技术,包括TBS(turf base seeding)植草(灌)防护和喷播养护策略,增加客土厚度和采用双层TBS镀锌网片加固等。实践表明,上述措施能显著提升植被的绿化成活率和边坡的稳定性。研究结果可为类似地质环境下的绿化防护工程的设计与施工提供有价值的参考。
