Carbon quantum dots(CQDs)are widely used in fluorescence imaging due to their negligible toxicity,low-cost and excellent Huorescence properties.The fluorescence properties of CQDs are greatly a fleeted by the synthesi...Carbon quantum dots(CQDs)are widely used in fluorescence imaging due to their negligible toxicity,low-cost and excellent Huorescence properties.The fluorescence properties of CQDs are greatly a fleeted by the synthesis method,passivation agent and reaction media.In this study,the nitrogen-doped CQDs(N-CQDs)were synthesized by a facile microwave-assisted method with citric acid(CA)as a carbon source and deep eutectic solvents as a N-dopaiit as well as the reaction solvent.After detailed characterizations,the as-prepared N-CQDs exliibited near spherical morphology with an average size of(2.64±0.55)nm.Besides,the abundant hydrophilic limctional groups including hydroxyl,carboxyl,amine and quaternary-ammonium were present on the surface of the N-CQDs.TheN-CQDs displayed the excitation-dependent fluorescence feature under the excitation wavelength of 350—80 nm.Moreover,the N-CQDs could be efficiently uptaken by RAW264.7 cells and emitted bright green light.Meanwhile,the resultant N-CQDs showed the low cytotoxicity in living cells,suggesting the great potential in bio-imaging applications.展开更多
基金Supported by the National Natural Science Foundation of China(No,21601068)the Scientific Research Foimdation for Advanced Talents of Jiangsu University,China(No.14JDG054).
文摘Carbon quantum dots(CQDs)are widely used in fluorescence imaging due to their negligible toxicity,low-cost and excellent Huorescence properties.The fluorescence properties of CQDs are greatly a fleeted by the synthesis method,passivation agent and reaction media.In this study,the nitrogen-doped CQDs(N-CQDs)were synthesized by a facile microwave-assisted method with citric acid(CA)as a carbon source and deep eutectic solvents as a N-dopaiit as well as the reaction solvent.After detailed characterizations,the as-prepared N-CQDs exliibited near spherical morphology with an average size of(2.64±0.55)nm.Besides,the abundant hydrophilic limctional groups including hydroxyl,carboxyl,amine and quaternary-ammonium were present on the surface of the N-CQDs.TheN-CQDs displayed the excitation-dependent fluorescence feature under the excitation wavelength of 350—80 nm.Moreover,the N-CQDs could be efficiently uptaken by RAW264.7 cells and emitted bright green light.Meanwhile,the resultant N-CQDs showed the low cytotoxicity in living cells,suggesting the great potential in bio-imaging applications.
