[Objective] The research aimed to study the toxicity and antifeedant activity of walnut green husk extract on wheat aphid. [Method] Using ultrasonic extraction, 5 kinds of different polarity solvents were used to prep...[Objective] The research aimed to study the toxicity and antifeedant activity of walnut green husk extract on wheat aphid. [Method] Using ultrasonic extraction, 5 kinds of different polarity solvents were used to prepare the extracts from walnut green husk. The toxicity and antifeedant activity on wheat aphid were determined, [ Result ] Walnut green husk extracts in the solvent of ethanol, acetone, ethyl acetate had higher toxicity and antifeedant activity on wheat aphid. When the concentration was 100 mg/ml, after 24 h, the toxicity and antifeedant activity of three kinds extracts on wheat aphid were greater than 84.35% and 66.98%, respectively. After 48 h, the toxicity and antifeedant activity were greater than 85.91% and 79.34%, respectively. Among 5 kinds extracts,ethanol extracts had the highest toxicity and antifeedant activity on wheat aphid. After 24 h, the corrected mortality rate was 93.64% and the antifeedant rate was 83.61%. After 48 h,the corrected mortality rate was 94.95% and antifeedant rate was 92.21%. Petroleum ether extract had the smallest toxicity and antifeedant activity on wheat aphid. After 24 h,the corrected mortality rate and antifeedant rate were 19.54% and 34.65%, respectively. After 48 h,the corrected mortality rate and antifeedant rate were 20.13% and 39. 03%, respectively. [ Conclsion] The extracts from walnut green husk with larger polarity solvent had better toxicity and higher antifeedant activity on wheat aphid.展开更多
The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity ...The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity （TAA）,superoxide anion radical scavenging activity （SARSA）,active oxygen scavenging activity （AOSA）,and DPPH （1,1-diphenyl-2 picrylhydrazyl free radical） radical scavenging activity.The results showed that the pigment had higher antioxidant activity and TAA,SARSA,AOSA and DPPH.scavenging activities at a certain concentration than Vc （antiscorbutic vitamin,vitamin C）,and the capacity increased with the increase of pigment concentration.Its TAA was 51.06 U mL-1,1.73 times of Vc,and SARSA 18 025.21 U mL-1,2.26% higher than Vc,and AOSA 3 776.31 U mL-1,1.24 times of Vc.As to the DPPH.scavenging activity of the pigment,there was a trend that higher concentration performed higher activity significantly improved with the company of Vc.The pigment showed significant antioxidant activities evaluated by different assays.Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.展开更多
The persistence and performance (growth promoting potential) of green fluorescent protein (gfp) marked Azotobacter chroococcum strain ABR 4G were studied in sterilized and unsterilized wheat rhizospheric soil. The gfp...The persistence and performance (growth promoting potential) of green fluorescent protein (gfp) marked Azotobacter chroococcum strain ABR 4G were studied in sterilized and unsterilized wheat rhizospheric soil. The gfp was integrated via Tn 5 transposition into A. chroococcum chromosome and the resultant gfp marked colonies were identified by green fluorescent emission under UV light. The gfp was stably maintained in A. chroococcum and the gfp insertion had no apparent adverse effect on the growth promoting properties of the marked soil isolate ABR 4G. The growth promoting properties (nitrogen fixation, ammonia excretion, phosphate solubilization and IAA production) of the parent soil isolate and the gfp marked strain were found to be almost the same. All the quantitative wheat plant traits were significantly influenced by inoculation of A. chroococcum ABR 4G strain in sterilized and unsterilized soil. Inoculated bacterial counts increased gradually in wheat rhizosphere, reached maximum on 60 th d and declined on 80 th d. Fertility levels also affected survival of marked strain and the survival was comparable in sterilized and unsterilized soil. The growth promoting properties were also determined from the marked strain reisolated from wheat rhizosphere in both types of soil. Fig 1, Tab 2, Ref展开更多
基金Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ09205)~~
文摘[Objective] The research aimed to study the toxicity and antifeedant activity of walnut green husk extract on wheat aphid. [Method] Using ultrasonic extraction, 5 kinds of different polarity solvents were used to prepare the extracts from walnut green husk. The toxicity and antifeedant activity on wheat aphid were determined, [ Result ] Walnut green husk extracts in the solvent of ethanol, acetone, ethyl acetate had higher toxicity and antifeedant activity on wheat aphid. When the concentration was 100 mg/ml, after 24 h, the toxicity and antifeedant activity of three kinds extracts on wheat aphid were greater than 84.35% and 66.98%, respectively. After 48 h, the toxicity and antifeedant activity were greater than 85.91% and 79.34%, respectively. Among 5 kinds extracts,ethanol extracts had the highest toxicity and antifeedant activity on wheat aphid. After 24 h, the corrected mortality rate was 93.64% and the antifeedant rate was 83.61%. After 48 h,the corrected mortality rate was 94.95% and antifeedant rate was 92.21%. Petroleum ether extract had the smallest toxicity and antifeedant activity on wheat aphid. After 24 h,the corrected mortality rate and antifeedant rate were 19.54% and 34.65%, respectively. After 48 h,the corrected mortality rate and antifeedant rate were 20.13% and 39. 03%, respectively. [ Conclsion] The extracts from walnut green husk with larger polarity solvent had better toxicity and higher antifeedant activity on wheat aphid.
基金supported by the National Technologies R&D Program of China (2006BAD01A02)the Excellent Medium-Youth Scientist Scientific Research Reward Fundation of Shandong Province, China (BS2009NY036)the Youth Science and Technology Creative Fundation Item of Shandong Agricultural University, China
文摘The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity （TAA）,superoxide anion radical scavenging activity （SARSA）,active oxygen scavenging activity （AOSA）,and DPPH （1,1-diphenyl-2 picrylhydrazyl free radical） radical scavenging activity.The results showed that the pigment had higher antioxidant activity and TAA,SARSA,AOSA and DPPH.scavenging activities at a certain concentration than Vc （antiscorbutic vitamin,vitamin C）,and the capacity increased with the increase of pigment concentration.Its TAA was 51.06 U mL-1,1.73 times of Vc,and SARSA 18 025.21 U mL-1,2.26% higher than Vc,and AOSA 3 776.31 U mL-1,1.24 times of Vc.As to the DPPH.scavenging activity of the pigment,there was a trend that higher concentration performed higher activity significantly improved with the company of Vc.The pigment showed significant antioxidant activities evaluated by different assays.Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.
文摘The persistence and performance (growth promoting potential) of green fluorescent protein (gfp) marked Azotobacter chroococcum strain ABR 4G were studied in sterilized and unsterilized wheat rhizospheric soil. The gfp was integrated via Tn 5 transposition into A. chroococcum chromosome and the resultant gfp marked colonies were identified by green fluorescent emission under UV light. The gfp was stably maintained in A. chroococcum and the gfp insertion had no apparent adverse effect on the growth promoting properties of the marked soil isolate ABR 4G. The growth promoting properties (nitrogen fixation, ammonia excretion, phosphate solubilization and IAA production) of the parent soil isolate and the gfp marked strain were found to be almost the same. All the quantitative wheat plant traits were significantly influenced by inoculation of A. chroococcum ABR 4G strain in sterilized and unsterilized soil. Inoculated bacterial counts increased gradually in wheat rhizosphere, reached maximum on 60 th d and declined on 80 th d. Fertility levels also affected survival of marked strain and the survival was comparable in sterilized and unsterilized soil. The growth promoting properties were also determined from the marked strain reisolated from wheat rhizosphere in both types of soil. Fig 1, Tab 2, Ref
