Aim To establish the method for determination of benzofuranones derivative, griffonilide, in the roots of Semiaquilegia adoxoides （DC.） Makino by RP- HPLC. Methods Kromasil C18 column was used with a mobile phase of...Aim To establish the method for determination of benzofuranones derivative, griffonilide, in the roots of Semiaquilegia adoxoides （DC.） Makino by RP- HPLC. Methods Kromasil C18 column was used with a mobile phase of methanol-water （ containing 0. 01% trifluoroacetic acid and 0. 05 % tetrahydrofuran） （3：97）. The detecting wavelength was 260 nm. Results The calibration curve was linear over the range of 0. 004 - 0. 4 mg·mL^-1 with the correlation coefficient of 1.00 000. The average recovery was 98. 77 % with RSD 2. 77 %. Determinations of ten samples from different areas showed that the contents of griffonilide in the roots of S. adoxoides are 0. 17 -0. 49 mg·g^-1. Conclusion The method provides an accurate and sensitive way to detect griffonilide in the roots of S. adoxoides .展开更多
基金Ministry of Science and Technology of People'sRepublic of China (No. 2004AA2Z3730)
文摘Aim To establish the method for determination of benzofuranones derivative, griffonilide, in the roots of Semiaquilegia adoxoides （DC.） Makino by RP- HPLC. Methods Kromasil C18 column was used with a mobile phase of methanol-water （ containing 0. 01% trifluoroacetic acid and 0. 05 % tetrahydrofuran） （3：97）. The detecting wavelength was 260 nm. Results The calibration curve was linear over the range of 0. 004 - 0. 4 mg·mL^-1 with the correlation coefficient of 1.00 000. The average recovery was 98. 77 % with RSD 2. 77 %. Determinations of ten samples from different areas showed that the contents of griffonilide in the roots of S. adoxoides are 0. 17 -0. 49 mg·g^-1. Conclusion The method provides an accurate and sensitive way to detect griffonilide in the roots of S. adoxoides .
