[Objective] The aim was to develop prevention and control approaches of diseases and pests of Siraitia grosvenori. [Method] Long-term observation was con- ducted on cultivation methods of Siraitia grosvenori in the ga...[Objective] The aim was to develop prevention and control approaches of diseases and pests of Siraitia grosvenori. [Method] Long-term observation was con- ducted on cultivation methods of Siraitia grosvenori in the gardens in Longjiang Vil- lage of Yongfu County to conclude the prevention methods. [Result] Siraitia grosvenori yield can be reduced by nine diseases, such as root knot nematode dis- ease, and eight pests, such as Brachytrupes portentosus. [Conclusion] It would be effective for prevention of diseases and pests and guarantee quality and yield of Luohan guo to manage the gardens dominated by biological control and supple- mented by pesticide as per crop rotation.展开更多
The traditional technique for asexual propagation of Siraitia grosvenori in vitro is adopted widely by pressing the vine, which has a high risk of carrying viral diseases and limits the production of promoted cultivar...The traditional technique for asexual propagation of Siraitia grosvenori in vitro is adopted widely by pressing the vine, which has a high risk of carrying viral diseases and limits the production of promoted cultivars. So this paper reported in vitro regeneration of S. grosvenori by testing for shoot induction from various explant sources such as leaf, petiole and stem. Several phytohormone combinations of TDZ, BA and IAA were examined for shoot regeneration and NAA or IBA for rooting. The highest shoot induction rate （100% of regeneration frequency and 15.3 shoots per explant） in leaf was obtained by incubation on MS medium supplemented with 0.5 mg·L^-1 TDZ, 2.0 mg·L^-1 BA and 0.5 mg·L^-1 IAA; unlike shoot regeneration in leaves, the most efficient bud inductions in petiole and stem explants were initiated on MS medium containing 0.2 mg·L^-1 TDZ, 2.0mg·L^-1 BA and 0.5 mg·L^-1 IAA, in addition, adventitious buds in petiole and stem explants needed to be transformed to MS medium for development; optimal root was obtained when shoots were cultured on 1/2MS medum supplemented with 0.5 mg·L^-1 NAA or 0.5 mg·L^-1 IBA.展开更多
文摘[Objective] The aim was to develop prevention and control approaches of diseases and pests of Siraitia grosvenori. [Method] Long-term observation was con- ducted on cultivation methods of Siraitia grosvenori in the gardens in Longjiang Vil- lage of Yongfu County to conclude the prevention methods. [Result] Siraitia grosvenori yield can be reduced by nine diseases, such as root knot nematode dis- ease, and eight pests, such as Brachytrupes portentosus. [Conclusion] It would be effective for prevention of diseases and pests and guarantee quality and yield of Luohan guo to manage the gardens dominated by biological control and supple- mented by pesticide as per crop rotation.
文摘The traditional technique for asexual propagation of Siraitia grosvenori in vitro is adopted widely by pressing the vine, which has a high risk of carrying viral diseases and limits the production of promoted cultivars. So this paper reported in vitro regeneration of S. grosvenori by testing for shoot induction from various explant sources such as leaf, petiole and stem. Several phytohormone combinations of TDZ, BA and IAA were examined for shoot regeneration and NAA or IBA for rooting. The highest shoot induction rate （100% of regeneration frequency and 15.3 shoots per explant） in leaf was obtained by incubation on MS medium supplemented with 0.5 mg·L^-1 TDZ, 2.0 mg·L^-1 BA and 0.5 mg·L^-1 IAA; unlike shoot regeneration in leaves, the most efficient bud inductions in petiole and stem explants were initiated on MS medium containing 0.2 mg·L^-1 TDZ, 2.0mg·L^-1 BA and 0.5 mg·L^-1 IAA, in addition, adventitious buds in petiole and stem explants needed to be transformed to MS medium for development; optimal root was obtained when shoots were cultured on 1/2MS medum supplemented with 0.5 mg·L^-1 NAA or 0.5 mg·L^-1 IBA.
