Genome Array on Differentially Expressed Genes of Skin Tissue in Cashmere Goat at Early Anagen of Cashmere Growth Cycle Using DNA Microarray

In order to study the molecular mechanism involved in cashmere regeneration, this study investigated the gene expression profile of skin tissue at various stages of the cashmere growth cycle and screen differentially expressed genes at proangen in 10 cashmere goats at 2 years of age using agilent sheep oligo microarray. Significance analysis of microarray （SAM） methods was used to identify the differentially expressed genes, Hierarchical clustering was performed to clarify these genes in association with different cashmere growth stages, and GO （Gene ontology） and the pathway analyses were con-ducted by a free web-based Molecular Annotation System3.0 （MAS 3.0）. Approximately 10200 probe sets were detected in skin tissue of 2-yr-old cashmere goat. After SAM analysis of the microarray data, totally 417 genes were shown to be differentially expressed at different cashmere growth stages, and 24 genes are significantly up-regulated （21） or down-regulated （3） at proangen concurrently compared to angen and telogen. Hierarchical clustering analysis clearly distinguished the differentially expressed genes of each stage. GO analysis indicated that these altered genes at proangen were predominantly involved in collagen fibril organization, integrin-mediated signaling pathway, cell-matrix adhesion, cell adhesion, transforming growth factor-β （TGF-β） receptor signaling pathway, regulation of cell growth. Kyoto encyclopedia of genes and genomes （KEGG） analysis showed that the significant pathways involved mainly included focal adhesion and extracellular matrixc （ECM）-receptor interaction. Some important genes involved in these biological processes, such as COL1A1, COL1A2, COL3A1, SPARC, CYR61 and CTGF, were related to tissue remolding and repairing and detected by more than one probe with similar expression trends at different stages of cashmere growth cycle. The different expression of these genes may contribute to understanding the molecular mechanism of cashmere regeneration.

ECONOMIC GROWTH AND CYCLES IN MODERN CHINA

If the theory of cyclical fluctuation can be applied to modern China, the economic development ofChina in this period can be seen as consisting of several stages. At each stage as well as in the wholeperiod, chosen index system is likely to help measure the growth rate. By vertical and horizontal com-parison, this paper will try to settle the disputes on China’s modern economic development.

STOCHASTIC MODELLING OF FATIGUE CRACK GROWTH UNDER CONSTANT AMPLITUDE LOADING

Two stochastic models of fatigue crack growth under constant amplitude cyclic loading are proposed and studied by using the stochastic averaging method and total probability theorem to account for high frequency component and low frequency component, respectively, of the irregular nature of fatigue crack growth observed in Virkler's experiment. Particular attention is paid to the prediction performance of the models under a change in the initial crack length. It is shown that the models proposed in the present paper yields better agreement with experimental data than other models available in literature.

Effects of insulin-like growth factor binding protein 7 on cell cycle and proliferation of gastric cancer in vitro and in vivo

Objective To investigate the effects of insulin-like growth factor binding protein 7(IGFBP7)on the proliferation,cell cycle of gastric cancer cell and the expression of cynlin D1,cyclin-dependent kinase(CDK)4,and to observe the effects of IGFBP7 on the growth of gastric tumor xenografts in nude mice.Methods The MKN-28cell line was interfered by small interfere ribonucleic acid(siRNA)(interfered group),and blank control group,

Research on the phase characteristics and driving mechanism of Chinese business cycle fluctuation--Empirical analysis based on MS-TVTP model

Based on the Keynesian IS curve and MS-TVTP model,this paper studies the phase operating characteristics and driving mechanism of Chinese business growth cycle fluctuation by specifying output gap series through coincident composite index and estimating expectation variable through state space model.The results are that the influences of output gap expectation on business growth cycle are positive and the effects of real interest rate are negative.Both of them exert asymmetric features in different regimes.The estimation results of smoothed probability and transition probability show that the financial and investment composite indexes are the significant driving factors of business growth cycle switch among different regimes.Compared to promoting economic growth,the monetary policy plays a more effective role in decelerating the growth speed.Although the speed of investment’s influence during the economic downturn phase is rapid,the lag of its effects is lengthened due to the investment cycle and capital formation.Releasing reform dividend through stabilizing expectation and reducing the fluctuation of business cycle by employing the macro prudent government are important ways to maintain the steady growth of Chinese economy.

Vegetative Storage Protein with Trypsin Inhibitor Activity Occurs in Sapindus mukorassi,a Sapindaceae Deciduous Tree

A vegetative storage protein （VSP） with trypsin inhibitor activity in a deciduous tree, Sapindus mukorassi, was characterized by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western-blot, immuno-histochemical localization, light- and electro-microscopy, together with analysis of proteinase inhibitor activity of the purified VSP in vitro. There were two proteins with molecular masses of about 23 and 27 kDa in a relatively high content in the bark tissues of terminal branches of S. mukorassi in leafless periods. The proteins decreased markedly during young shoot development, indicating their role in seasonal nitrogen storage. Immuno-histochemical localization with the polyclonal antibodies raised against the 23 kDa protein demonstrated that the 23 kDa protein was the major component of protein inclusions in protein-storing cells. The protein inclusions were identified by protein-specific staining and should correspond to the electron-dense materials in different forms in the vacuoles of phloem parenchyma cells and phloem ray parenchyma cells under an electron microscope. So, the 23 kDa protein was a typical VSP in S. mukorassi. The 23 and 27 kDa proteins shared no immuno-relatedness, whereas the 23 kDa protein was immuno-related with the 22 kDa VSP in lychee and possessed trypsin inhibitor activity. The 23 kDa protein may confer dual functions： nitrogen storage and defense.