HEPATOCYTE GROWTH FACTOR PROTECTS AGAINST APOPTOSIS INDUCED BY ADVANCED GLYCATION END PRODUCTS IN ENDOTHELIAL CELLS

Objective To investigate the effects of hepatocyte growth factor(HGF)on vascular endothelial cells apoptosis induced by advanced glycation end products(AGEs)and its possible mechanism. Methods Human umbilical vein endothelial cells(HUVECs)were cultured in vitro and intervened by different concentrations of AGEs and HGF.The cell inhibitory rates of each group with different culture time(12, 24, 48, and 72 hours)were measured by methyl thiazolyl tetrazolium(MTT)assay. The early stage apoptosis was detected by flow cytometry with Annexin V-FITC/PI double staining, morphology of cell apoptosis was observed by hoechst 33258 fluorescence staining, and the expression of apoptosis-associated genes Bax and Bcl-2 were determined by Western blotting.The activity of caspase-3 was detected by enzyme-linked immunosorbent assay (ELISA).Results Morphological observation indicated that high concentration of AGEs induced characteristic apoptotic changes in HUVECs.Within a certain concentration range, HUVECs apoptosis inducing rates by AGEs were in both dose- and time-dependent manners.HGF significantly inhibited the apoptosis of HUVECs induced by AGEs (P< 0.05).AGEs significantly promoted expression of Bax protein, but not Bcl-2.Whereas HGF significantly promoted the expression of Bcl-2(P<0.01)and decreased the activity of caspase-3(P<0.05)without affecting Bax level.Conclusions AGEs can induce the apoptosis of endothelial cells in vitro.HGF may effectively attenuate AGEs-induced endothelial cells apoptosis through upregulating Bcl-2 gene expression and inhibiting caspase-3 activation.

Increased glycated basic fibroblast growth factor in diabetic skin reduces the cell viability and angiogenesis of human dermal microvascular endothelial cells

Objective To explore the glycation of basic fibroblast grow th factor( bFGF) in diabetic skin.Methods The abdom inal full-thickness skin tissues from 58 patients( 29 diabetic and 29 non-diabetic) aged 40 to69 years and granulation tissues from 15 patients( 8 diabetic and 7 non-diabetic) aged 50 to 59 were analyzed.The proportion of advanced glycation end products( AGEs)-b FG F intotal b FGF was measured with co-immunoprecipitation and the histological characteristics of wound skin were detected with hem atoxylin and eosin staining. The cell viability, apoptosis, and angiogenesis of human derm al m icrovascular endothelial cells( HDMECs) after exposure to AG Es-b FG For bFGF were measured with cell counting kit-8, flow cytom etry,and tube form ation assay, respectively. Results The proportion of AG Es-b FG F in total b FG F showed agedependent increaseboth in diabetic and non-diabetic skin. As com pared with non-diabetic skin, the constituent ratio in diabetic skin increased significantly in the equal age-group, and the same result could be obtained in granulation tissues from patients aged 50 to 59. The proportion of AG Es-b FG F in diabetic granulation was low er than that in diabetic skin from patients aged 50 to 59. H istological analysis show ed few er vessels in diabetic skin wound. In vitro, the viability and vascularization of H D M EC s were promoted by b FG F and inhibited after exposure to AGEs-bFGF for 7d. Conclusion The present study indicates that one cause for im paired wound healing in diabetic skin could be the glycated b FG F and its changed angiogenic function.

Association of vascular endothelial growth factor-634G/C and receptor for advanced glycation end products G82S gene polymorphisms with diabetic retinopathy

AIMTo investigate the association of receptor for advanced glycation end products (RAGE) G82S and vascular endothelial growth factor (VEGF) -634 G/C gene polymorphisms with diabetic retinopathy (DR).METHODSOur cross-sectional study included 61 diabetic patients, 12 of them had proliferative diabetic retinopathy (PDR), 15 had non proliferative diabetic retinopathy (NPDR), 34 had no diabetic retinopathy (NDR) and 61 healthy controls. Participants were tested for RAGE G82S and VEGF -634 G/C polymorphisms by polymerase chain reaction-restriction fragment length polymorphism.RESULTSWe found a significant association between VEGF -634 G/C polymorphism and PDR as PDR patients had increased incidence of VEGF -634 CC genotype compared to NDR patients [odds ratio for CC vs (GC+GG)=6.5, 95% CI=1.5-27.8, P=0.021]. Also VEGF -634 CC genotype and C allele were significantly higher in the PDR than in NPDR patients, which is a novel finding in our study (P=0.024, 0.009 respectively). The mean triglycerides level was significantly higher in diabetic patients with CC genotype (P=0.01) as compared to patients with other genotypes. All cases and control subjects were of the same heterozygous RAGE 82G/S genotype.CONCLUSIONPatients carrying VEGF -634 C polymorphism have a higher risk of PDR development, so VEGF -634 G/C polymorphism could be used as a predictive marker for PDR in diabetic patients. We could not find a significant association between RAGE G82S polymorphism and DR.

新生班级如何开好头起好步?——基于X校价值观实验的探索

X校开展的社会主义核心价值观落细落小落实到班级的实验充分证明了良好的开端是成功的一半的道理。新生班级开好头起好步,对全班同学在校几年的成长成才具有奠基定向意义。坚持全过程人民民主,相信、尊重和依靠学生,引导新生明晰并记录上学初心、谋求校园生活的增值性发展和可视化成长,以终为始,引导形成勤学互助奉献的班级核心价值观(集体目标)与改掉一个坏习惯的个人目标,协商制定守住安全、健康与文明言行底线的“四大公约”(寝室、班级、校园、网络),讨论达成目标和守住底线可为有效的举措,设计成效检测的指标与方法等。使同学们从入学之初就明确大学生活的共同价值与言行目标以及达成这一目标的方法,营造互帮互助、互教互学、互促共进的班风学风,一张蓝图绘到底,一年接着一年干,锁定目标,师生心往一处想,力往一处使,持续发力,久久为功。

子痫风险预测联合脐动脉S/D比值对妊娠期糖尿病患者妊娠结局的预测价值

目的探讨子痫风险预测联合脐动脉收缩期峰值流速与舒张末期流速(peak systolic velocity/end diastolic velocity,S/D)比值对妊娠期糖尿病患者妊娠结局的预测价值。方法选取2021年1月至2023年1月笔者医院产科门诊收治的60例妊娠期糖尿病(gestational diabetes mellitus,GDM)患者作为妊娠糖尿病组;同一时间,选取笔者医院产科门诊孕检正常的健康孕妇30例作为对照组。采用磁微粒化学发光法检测血清胎盘生长因子(placental growth factor,PIGF),超声检测,S/D比值同时按照是否合并不良妊娠结局分为妊娠结局良好组及不良妊娠结局组,比较PIGF及S/D比值;采用受试者操作特征(receiver operating characteristic,ROC)曲线分析PIGF联合S/D比值对GDM患者妊娠结局的预测价值进行预测分析。结果与对照组比较,妊娠糖尿病组患者PIGF降低,S/D升高(P<0.05)。对照组不良妊娠结局的发生率为20.00%,妊娠糖尿病组不良妊娠结局的发生率为46.67%,与对照组比较,妊娠糖尿病组不良妊娠结局的发生率升高(P<0.05)。与妊娠结局良好组比较,不良妊娠结局组的GDM患者PIGF水平降低,S/D比值升高(P<0.05)。ROC曲线分析PIGF、S/D比值对GDM患者不良妊娠结局敏感度及特异性分析发现,S/D比值诊断效果高于PIGF,但联合更高。结论妊娠期糖尿病患者中PIGF水平降低,S/D比值增加,且与患者不良妊娠结局相关,PIGF联合S/D比值对患者不良妊娠结局的诊断效能最高。

Features of Phenological Phases of Blackberry in the Conditions of the Samarkand Region

The article presents data on the study of phenological phases of blackberries in the Samarkand region in 2022-2023. The influence of differing in weather conditions, especially at the beginning and end of the growing season, on the timing of onset and duration of the main phenophases of the plant is analyzed. The objects of the study were 4 varieties of different genetic and ecological-geographical origin of foreign selection: Brazos, Jumbo, Thornfree and Karaka Black. Phenological observations were carried out according to the classical methodology developed for this crop in Russia. The timing of all phenophases was significantly dependent on the temperature conditions of the growing season: in the year with a higher sum of average daily temperatures, the corresponding phenophases occurred earlier. According to preliminary data, the climatic indicators of the vegetation period of this region allow for the cultivation of blackberries of the main morphological groups (erect, semi-erect, trailing) and different ripening periods.

塞罕坝木质部生长物候与植被物候的差异及影响因素

为探究塞罕坝地区木质部生长物候与植被物候变化规律及其影响因子,基于塞罕坝样点数据的木质部生长物候监测数据及NDVI数据利用最佳阈值提取方法获得预测木质部生长物候的最佳阈值,并基于该阈值预测塞罕坝地区2010-2021年木质部起止生长物候。利用动态阈值法基于遥感数据提取植被物候,对比分析木质部生长物候与植被物候空间变化差异及其影响因子。结果表明,塞罕坝地区木质部生长始期主要集中在每年第111~150天,木质部生长末期主要集中在每年第235~257天。植被木质部生长季长度大约为96~130 d。塞罕坝地区木质部生长物候比植被物候起始期提前3~21 d,该差异主要受温度降水和坡度影响,而生长结束期延后3~43 d,该差异主要受植被覆盖度影响。木质部生长季长度比植被物候长9~64 d,主要受降水的影响。由于木质部生长比植被物候更能反映树木碳汇的形成时期,因此更长的木质部生长物候表明更多的碳积累。

Bio-Oss骨粉与CGF在自体牙移植游离端骨缺损中的修复重建

目的评估Bio-Oss骨粉和浓缩生长因子(concentrated growth factor,CGF)联合应用于自体牙移植游离端骨缺损修复的临床疗效。方法选取2018年7月至2022年6月就诊于合肥市口腔医院口腔外科患者共44例,观察组23例,术中应用Bio-Oss骨粉及CGF;对照组21例,不应用任何材料,术后定期随访,对比移植牙术后即刻初期稳定性、术后1周牙龈状况、术后3个月和6个月骨愈合情况以及术后1年成功率。结果观察组移植牙术后即刻初期稳定性较对照组有明显改善,差异有统计学意义(P<0.05);观察组术后1周牙龈肿胀度与对照组无明显改善,差异无统计学意义(P>0.05);术后3个月受牙区牙槽骨愈合情况较对照组好,差异有统计学意义(P<0.05);术后随访1年,观察组牙移植成功率100%;对照组牙移植成功率90.5%,留存率95.2%,失败率4.8%,差异有统计学意义(P<0.05)。结论Bio-Oss骨粉与CGF在自体牙移植游离端骨缺损中有较好的临床疗效,促进新骨形成,有利于移植牙早期稳定,提高自体牙移植成功率。

血清成纤维生长因子-23/抗衰老基因Klotho、辅助性T淋巴细胞1/辅助性T淋巴细胞2细胞因子在老年终末期肾病患者中的表达及对医院感染的预测和预后的影响

目的观察血清成纤维生长因子-23(fibroblast growth factor 23,FGF23)/抗衰老基因Klotho、辅助性T淋巴细胞(helper T lymphocytes,Th)1/Th2细胞因子在老年终末期肾病患者中的表达,并分析对医院感染的预测和预后的影响。方法选取2021年1月1日至2022年12月31日自贡市精神卫生中心(自贡市老年病医院)收治的106例老年终末期肾病患者,男65例,年龄范围60~84岁,年龄(67.21±3.07)岁,女41例,年龄范围60~84岁,年龄(67.65±2.98)岁,于入院第2天检测血清FGF23、抗衰老基因Klotho、Th1细胞因子[γ干扰素(interferon-γ,IFN-γ)、白介素2(interleukin-2,IL-2)]、Th2细胞因子(IL-4、IL-10)水平,进行急性生理功能及慢性健康状况评分Ⅱ(acute physiology and chronic health status scoring systemⅡ,APACHEⅡ)评估。根据住院期间是否发生医院感染分为感染组(28例)、未感染组(78例),比较两组各项血清指标及APACHEⅡ评分水平,分析各项指标与APACHEⅡ评分的相关性。随访3个月统计患者生存预后[生存(71例)、死亡(35例)],比较不同预后患者血清FGF23、Klotho、IFN-γ、IL-2、IL-4、IL-10水平,分析各项指标对医院感染及预后的预测价值及临床效用。结果106例老年终末期肾病患者根据住院期间是否发生医院感染分为感染组(28例)、未感染组(78例),随访3个月后生存71例、死亡35例。入院第2天感染组血清FGF23、IL-4、IL-10水平及APACHEⅡ评分分别为(78.64±20.16)ng/mL、(20.14±1.48)μg/L、(22.47±2.56)μg/L、(26.38±6.51)分,未感染组分别为(60.17±16.83)ng/mL、(16.25±1.21)μg/L、(19.52±1.86)μg/L、(22.97±6.45)分,感染组血清FGF23、IL-4、IL-10水平及APACHEⅡ评分均高于未感染组(P<0.05);入院第2天感染组血清Klotho、IFN-γ、IL-2水平分别为(34.95±12.62)ng/mL、(22.19±1.69)μg/L、(28.73±2.95)μg/L,未感染组分别为(51.61±16.08)ng/mL、(25.31±1.74)μg/L、(33.95±1.52)μg/L,感染组血清Klotho、IFN-γ、IL-2水平均低于未感染组(P<0.05);血清FGF23、IL-4、IL-10水平与APACHEⅡ评分呈正相关(相关系数r=0.629、0.597、0.612,P均<0.05),血清Klotho、IFN-γ、IL-2水平与APACHEⅡ评分呈负相关(相关系数r=-0.632、-0.718、-0.701、0.597,P均<0.05);死亡患者入组后1个月血清FGF23、IL-10、IL-4水平分别为(77.49±21.85)ng/mL、(24.76±4.77)μg/L、(24.81±6.28)μg/L,生存患者分别为(58.92±16.94)ng/mL、(18.10±3.82)μg/L、(13.57±4.38)μg/L,死亡患者入组后1个月血清FGF23、IL-10、IL-4水平高于生存患者(P<0.05);死亡患者入组后1个月血清Klotho、IFN-γ、IL-2水平分别为(30.03±11.76)ng/mL、(20.33±2.63)μg/L、(27.19±4.91)μg/L,生存患者分别为(55.68±17.02)ng/mL、(26.54±4.79)μg/L、(35.22±5.64)μg/L,死亡患者入组后1个月血清Klotho、IFN-γ、IL-2水平低于生存患者(P<0.05);血清FGF23、Klotho、IFN-γ、IL-2、IL-4、IL-10预测终末期老年肾病患者发生医院感染、生存预后曲线下面积分别为0.904(OR:0.832~0.953)、0.911(OR:0.840~0.958),且具有良好临床效用(P<0.05)。结论老年终末期肾病患者血清FGF23/Klotho、Th1/Th2细胞因子水平失衡,合并感染患者血清FGF23、IL-10、IL-4水平异常升高,血清Klotho、IFN-γ、IL-2水平表达降低,联合血清FGF23/Klotho、Th1/Th2细胞因子预测医院感染、评估预后的价值较高。

终末期肾脏病患者血清FGF23与心衰及死亡发生风险的前瞻性队列研究

目的探讨终末期肾脏病(ESRD)患者成纤维细胞生长因子-23(FGF23)血清浓度与心力衰竭及全因死亡的相关性。方法采用前瞻性队列研究,纳入医院肾脏内科收治的无心衰症状的ESRD患者,采用基线问卷和体格检查、超声心动图检查、实验室检查收集患者数据,采用酶联免疫吸附法(ELISA)检测患者血清FGF23浓度。随访时间2年,以随访发生新发的心力衰竭(ACC/AHA stage C-D)和全因死亡为复合终点结局事件,采用Cox比例风险模型分析患者发生结局事件的危险因素,通过亚组分析和交互作用分析,进一步探讨FGF23与结局事件的关联在不同亚组中是否存在异质性。结果该研究最终纳入ESRD患者107例,平均年龄(52.00±12.51)岁,男性39(36.45%)例,中位随访时间为23个月(21,25个月),出现结局事件32(29.9%)例,其中新发心衰22(20.6%)例,全因死亡10(9.3%)例。该研究结果显示结局事件组患者血清FGF23浓度显著高于非事件组[(4.40±1.16)pmol/ml vs(3.85±0.82)pmol/ml,P<0.05]。Cox比例风险模型结果显示升高的FGF23可以增加ESRD患者发生结局事件的风险(HR=1.730,95%CI:1.164~2.570,P=0.007)。亚组分析显示FGF23水平与性别对于结局事件发生风险存在交互作用,尤其在男性ESRD患者中升高的FGF23风险更高(P_(-交互作用)<0.05)。结论升高的血清FGF23是ESRD患者发生心衰和全因死亡的独立危险因素,尤其在男性患者中风险更高。

miRNA-27、FGF21及AGE在2型糖尿病血清中的表达及其与微血管并发症的相关性

目的 探讨2型糖尿病(T2DM)患者血清中miRNA-27、纤维细胞生长因子21(FGF21)及糖化终末产物(AGE)的表达,并分析它们与微血管并发症的相关性。方法 回顾性选取2020年2月至2022年2月在沧州市人民医院进行诊疗的89例2型糖尿病患者纳入研究组,根据患者是否合并微血管并发症(包括糖尿病所致的周围神经病变、肾脏病变和视网膜病变)将其分为合并组(n=41)和未合并组(n=48)。另选50例在本院进行健康体检的志愿者纳入对照组。比较研究组和对照组的miRNA-27、FGF21及AGE水平;比较合并组和未合并组患者的miRNA-27、FGF21、AGE水平、生化指标[总胆固醇、甘油三酯、甘油三酯、高密度脂蛋白(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、尿酸、血肌酐及尿素氮]水平,并探究影响患者合并微血管并发症的影响因素。结果 研究组的miRNA-27、FGF21、AGE水平分别为2.31±0.42、(388.27±23.09) ng/mL、(89.29±4.98) UA,均高于对照组[0.89±0.31、(268.56±26.18) ng/mL、(70.22±5.16) UA],差异均有统计学意义(P<0.05)。合并组患者的miRNA-27、FGF21、AGE水平分别为2.45±0.44、(399.26±25.49) ng/mL、(92.34±4.28) UA,均高于未合并组[1.98±0.36、(316.44±23.91) ng/mL、(76.28±3.92) UA],差异均有统计学意义(P<0.05)。两组间总胆固醇、LDL-C水平比较,差异均无统计学意义(P>0.05);合并组患者的HDL-C水平为(1.12±0.33) mmo/L,低于未合并组[(1.28±0.36) mmo/L],甘油三酯、尿酸、血肌酐、尿素氮水平分别为(2.33±0.51) mmo/L、(355.26±41.08)μmol/L、(92.08±9.04)μmol/L、(7.99±2.15) mmol/L,均高于未合并组[(1.98±0.46) mmo/L、(336.11±45.26)μmol/L、(72.45±9.87)μmol/L、(5.06±1.03) mmol/L],差异均有统计学意义(P<0.05)。多因素Logistic回归分析,可知FGF21、AGE、miRNA-27均为2型糖尿病合并微血管并发症产生的影响因素(P<0.05)。结论 2型糖尿病患者的血清中miRNA-27、FGF21和AGE水平较高,且在合并微血管并发症的2型糖尿病患者中表达更高,与2型糖尿病的微血管并发症的发生具有一定的相关性。

外延生长设备EFEM系统设计

阐述了应用于外延生长设备的EFEM系统,通过采用顶升机构、机械手和校准器等部件,结合机器视觉辅助判断方法,设计了一种外延生长设备的EFEM系统,该系统有效地提高了晶圆洁净度,提升了设备的自动化水平;并在相关机型进行应用和测试,能够可靠高效地运行。

2型糖尿病合并脑梗死患者血清FGF21、RAGE、DKK1表达情况及其意义

目的探讨2型糖尿病(T2DM)合并脑梗死患者血清成纤维细胞生长因子(FGF)21、晚期糖基化终末产物受体(RAGE)及Dickkopf-1蛋白(DKK1)表达情况及其意义。方法前瞻性选取2021年6月至2023年6月于中部战区总医院治疗T2DM合并脑梗死患者300例纳入试验组,选择同期于本院接受治疗的T2DM患者300例纳入T2DM组,选择同期于本院进行体检的健康者300名纳入对照组。检测并比较3组血清FGF21、RAGE、DKK1表达水平;分析血清FGF21、RAGE、DKK1对T2DM合并脑梗死的诊断价值;分析不同预后T2DM合并脑梗死患者临床资料和血清FGF21、RAGE、DKK1表达水平;分析血清FGF21、RAGE、DKK1与T2DM合并脑梗死预后的相关性;采用多因素Logistic回归分析分析血清FGF21、RAGE、DKK1对T2DM合并脑梗死预后的预测价值。结果试验组血清FGF21、RAGE、DKK1表达水平分别为(3.45±0.36)ng/L、(789.74±80.57)μg/mL、(98.76±9.92)ng/mL,均大于T2DM组[(2.68±0.29)ng/L、(578.06±59.84)μg/mL、(44.76±4.65)ng/mL]及对照组[(1.52±0.17)ng/L、(289.75±30.38)μg/mL、(24.95±2.64)ng/mL],差异均有统计学意义(P<0.05)。经受试者工作特征(ROC)曲线分析,血清FGF21、RAGE及DKK1联合诊断T2DM合并脑梗死的临床价值高于血清FGF21、RAGE及DKK1单一诊断的临床价值。预后不良患者和预后良好患者的性别构成比、年龄、体重指数、随机血糖比较,差异均无统计学意义(P>0.05);预后不良患者的糖化血红蛋白(HbA1c)、FGF21、RAGE、DKK1分别为(8.42±0.86)%、(3.89±0.40)ng/L、(865.64±89.43)μg/mL、(125.64±14.26)ng/mL,均大于预后良好患者[(7.04±0.73)%、(3.11±0.33)ng/L、(735.08±75.07)μg/mL、(83.64±8.61)ng/mL],差异均有统计学意义(P<0.05)。经Spearman相关分析,血清FGF21、RAGE、DKK1与T2DM合并脑梗死预后均呈负相关(r=-0.624、-0.553、-0.726,P<0.05)。经Logistic回归分析,结果显示HbA1c、FGF21、RAGE、DKK1均为影响T2DM合并脑梗死预后的独立危险因素(P<0.05)。结论血清FGF21、RAGE、DKK1联合诊断T2DM合并脑梗死具有较高的诊断及预后价值,可将其应用于T2DM合并脑梗死患者的临床诊断及预后评价。

A method for determining vegetation growth process using remote sensing data: A case study in the Three-River Headwaters Region, China

Accurate measurements of the associated vegetation phenological dynamics are crucial for understanding the relationship between climate change and terrestrial ecosystems. However, at present, most vegetation phenological calculations are based on a single algorithm or method. Because of the spatial, temporal, and ecological complexity of the vegetation growth processes, a single algorithm or method for monitoring all these processes has been indicated to be elusive. Therefore, in this study, from the perspective of plant growth characteristics, we established a method to remotely determine the start of the growth season(SOG) and the end of the growth season(EOG), in which the maximum relative change rate of the normalized difference vegetation index(NDVI) corresponds to the SOG, and the next minimum absolute change rate of the NDVI corresponds to the EOG. Taking the Three-River Headwaters Region in 2000–2013 as an example, we ascertained the spatiotemporal and vertical characteristics of its vegetation phenological changes. Then, in contrast to the actual air temperature data, observed data and other related studies, we found that the SOG and EOG calculated by the proposed method is closer to the time corresponding to the air temperature, and the trends of the SOG and EOG calculated by the proposed method are in good agreement with other relevant studies. Meantime, the error of the SOG between the calculated and observed in this study is smaller than that in other studies.

Al-9Si合金中共晶Si生长机制研究

亚共晶Al-Si合金中,Si相的生长机制已经有很多研究,但至今仍然存在分歧。利用扫描电镜、透射电镜等手段研究了不同冷却速率下亚共晶Al-Si合金中Si相的三维形貌,以及Si生长末端的形貌及其微观结构。结果表明,随着冷却速率的提高,共晶Si的尺度进一步减小,分枝更为发达,但仍呈板片状。共晶Si的生长末端的特征主要有棱面端部、凹角端部、小面端部。对小面端部的衍射花样分析并没有典型的双斑点衍射花样,因此,提出了台阶凹角机制来解释共晶Si片状生长行为。

孟德尔随机化法探究TGF-β与AAMI后左室舒张末容积的关系

目的采用孟德尔随机化方法(MR)探究转化生长因子-β(TGF-β)水平与急性前壁心肌梗死(AAMI)急性期后左室舒张末容积(LVEDV)的因果关系。方法从基因组和表观基因组关联研究(GWAS/EWAS)数据库选取TGF-β和AAMI后LVEDV数据。TGF-β数据包括982例样本,LVEDV数据包括35 407例样本。选择与TGF-β独立相关的显著基因位点作为工具变量,分别使用逆方差加权法(IVW)、加权中位数、加权模式法及MR-Egger回归研究TGF-β与AAMI急性期后LVEDV的关系。结果逆方差加权法(IVW)法结果显示,TGF-β升高能增加AAMI后LVEDV扩大的风险(OR=1.021,95%CI:1.007~1.035,P=0.004)。与加权中位数法(OR=1.017,95%CI:1.008~1.026,P=2.26×10~(-4))及加权模式法(OR=1.014,95%CI:1.000~1.028,P=0.043),结果一致。MR-Egger回归截距为-0.002(95%CI:-0.018~0.014,P=0.791),表明工具变量几乎不存在多效性。散点图及漏斗图均提示,研究结果稳定可靠。结论TGF-β水平与AAMI急性期后LVEDV存在正向因果效应,即TGF-β升高能导致AAMI急性期后LVEDV扩大。

Egr3、sRAGE及升主动脉弹性功能在冠心病中的变化及与病变程度的关系

目的探讨早期生长反应因子3(Egr3)、可溶性糖基化终产物受体(sRAGE)及升主动脉弹性功能在冠心病中的变化及与病变程度的关系。方法选取2020年1月至2023年5月在新乡医学院第三附属医院治疗的冠心病患者100例作为观察组,其中急性冠脉综合征患者60例,稳定型心绞痛患者40例。同时选取健康志愿者100例作为对照组。检查观察组和对照组外周血Egr3、sRAGE水平,同时分析观察组不同疾病类型、狭窄程度患者升主动脉参数差异。结果观察组外周血Egr3高于对照组,而sRAGE低于对照组(P<0.05)。观察组急性冠脉综合征患者Egr3高于稳定型心绞痛患者,而sRAGE低于稳定型心绞痛患者(P<0.05)。观察组重度狭窄患者Egr3高于轻度和中度狭窄患者,而sRAGE低于轻度和中度狭窄患者(P<0.05)。观察组急性冠脉综合征患者升主动脉僵硬指数高于稳定型心绞痛,而升主动脉扩张性和前壁S波速度低于稳定型心绞痛患者(P<0.05)。观察组中度和重度狭窄患者升主动脉僵硬指数高于轻度狭窄患者(P<0.05),观察组中度和重度狭窄患者升主动脉扩张性低于轻度狭窄患者(P<0.05)。观察组重度狭窄患者前壁S波速度低于轻度和中度狭窄患者(P<0.05)。外周血Egr3与Gensini评分呈正相关,sRAGE、前壁S波速度与Gensini评分呈负相关(P<0.05)。结论冠心病患者外周血Egr3、sRAGE升高,其中Egr3、sRAGE和升主动脉弹性参数与疾病类型、狭窄程度有关;Egr3、sRAGE、前壁S波速度与Gensini评分存在相关性。

骨髓间充质干细胞旁分泌KGF对LPS诱导小鼠肺上皮细胞损伤的修复作用

目的探讨急性呼吸窘迫综合征(ARDS)时骨髓间充质干细胞(BMSC)旁分泌角质细胞生长因子(KGF)对脂多糖(LPS)损伤的肺泡上皮的修复作用,以及肺泡上皮细胞的晚期糖基化终末产物受体(RAGE)在BMSC旁分泌KGF修复LPS诱导的肺泡上皮损伤中的作用。方法建立LPS诱导的小鼠肺上皮细胞(MLE12)损伤模型以及体外BMSC与MLE12间接共培养模型。实验对象分为control、LPS、MSC-control、MSC-LPS、KGF及anti-RAGE组,培养1、7 d后,应用蛋白质印迹方法测定MLE12细胞中RAGE及闭锁小带蛋白-1(ZO-1)的含量,应用酶联免疫吸附试验方法测定细胞条件培养液中KGF及RAGE的含量。结果BMSC能增加LPS损伤肺上皮的ZO-1蛋白表达(F=172.40、146.80,P<0.01;t=14.91~67.40,P<0.05)。BMSC修复LPS损伤的肺上皮时条件培养液中KGF表达增加(F=45.51、2359.00,P<0.01;t=14.87~73.25,P<0.05);MLE12细胞的RAGE表达增加,差异有统计学意义(F=49.90~275.60,P<0.01;t=11.02~57.84,P<0.05)。结论BMSC对LPS诱导的MLE12细胞损伤有修复作用,BMSC修复LPS诱导的MLE12细胞损伤可能有旁分泌KGF的参与并通过调节RAGE表达实现。

经济增长目标对企业高级人才流动的影响研究

地方政府依据制定的经济增长目标开展经济建设,影响区域资源配置及包括企业高级人才流动在内的微观企业行为。以中国资本市场2008—2019年A股上市公司为样本,实证检验地方经济增长目标与企业高级人才流动之间的关系。研究发现:经济增长目标与企业高级人才流入之间存在倒U型关系,表现为随着地方经济增长目标的提高,当地上市公司聘请的博士学历高级人才呈现先增后减的趋势。异质性分析表明,这一非线性关系主要存在于大规模企业和西部地区的企业。因此,地方政府应合理制定经济增长目标,助推企业吸纳高级人才,以实现经济高质量发展。

管翅式换热器迎风侧翅片末端霜层生长模拟研究

基于管翅式换热器迎风侧翅片末端存在霜层生长的现象,建立其迎风侧翅片末端霜层生长模型。模拟结果表明,霜层沿气流反方向生长速度最快,且其生长速度随与气流反方向夹角θ的增大而不断减缓,霜层密度及霜层表面温度亦随着θ的增大不断减小,霜层整体呈流线型。3600 s后,θ=0°处的霜层厚度及密度分别是θ=90°处值的1.47倍和1.23倍,前者霜层表面温度比后者高2.2℃。研究成果有望指导提升管翅式换热器的防霜性能。