为了分析甘南牦牛(Bos grunniens)肌肉萎缩盒蛋白32(F-box protein 32,FBXO32)基因的单核苷酸多态性(single nucleotide polymorphism,SNP)位点,以及基因型与胴体和肉质性状间的相关性,本研究以593头甘南牦牛为研究对象,采用混池测序和...为了分析甘南牦牛(Bos grunniens)肌肉萎缩盒蛋白32(F-box protein 32,FBXO32)基因的单核苷酸多态性(single nucleotide polymorphism,SNP)位点,以及基因型与胴体和肉质性状间的相关性,本研究以593头甘南牦牛为研究对象,采用混池测序和竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)技术,检测了甘南牦牛FBXO32基因突变位点及基因型,分析了基因型与甘南牦牛胴体及肉质性状的相关性。结果表明,从甘南牦牛FBXO32基因检测到7个SNP位点,分别是位于5′UTR区的SNP1(g.267A>C)、外显子1区的SNP2(g.326G>T)、外显子8区的SNP3(g.31231G>C),以及3′UTR区的SNP4(g.31352G>A)、SNP5(g.31424C>T)、SNP6(g.31503A>C)和SNP7(g.31504A>G)。其中,SNP1、SNP4、SNP5、SNP6与肌肉嫩度显著相关(P<0.05),SNP3、SNP6、SNP7与失水率显著相关(P<0.05),SNP1、SNP2、SNP3、SNP4、SNP5、SNP7与眼肌面积显著相关(P<0.05),SNP2、SNP5、SNP6与胴体重显著相关(P<0.05)。7个SNP位点构建的9种有效单倍型组合中,单倍型组合H2H2的个体肌肉嫩度小、熟肉率高,单倍型组合H1H2的个体熟肉率高、眼肌面积大、失水率低。FBXO32基因突变位点可作为甘南牦牛胴体及肉质性状潜在分子遗传标记,研究结果丰富了甘南牦牛经济性状分子遗传理论基础。展开更多
建立了毛细管电泳(CE)测定乳中乳清酸含量的方法。用三氯乙酸提取昌台牦牛(Bos grunniens)和两种品牌液态乳脱脂乳中的乳清酸,然后用毛细管电泳进行分离和定量。对试验条件(缓冲液浓度、p H等)进行了优化,乳清酸色谱保留时间为15 min左...建立了毛细管电泳(CE)测定乳中乳清酸含量的方法。用三氯乙酸提取昌台牦牛(Bos grunniens)和两种品牌液态乳脱脂乳中的乳清酸,然后用毛细管电泳进行分离和定量。对试验条件(缓冲液浓度、p H等)进行了优化,乳清酸色谱保留时间为15 min左右,最小检测限为0.02 mg/100 m L,在检测范围内线性关系良好(R2=0.999),回收率为99.66%。试验共测定了12头处于泌乳中期的昌台牦牛乳中的乳清酸含量,平均含量为(5.2±2.4)mg/100 m L,个体间含量差异较大。两种商业品牌的液态乳中乳清酸的含量平均为7.4 mg/100 m L,其中冷藏乳中乳清酸含量均高于常温乳,牦牛乳中的乳清酸含量低于商业液态乳。展开更多
Primers based upon the mitochondrial genome sequences of Bos taurus were used to amplify and sequence the complete mitochondrial D-loop region of Jiulong yak(Poephagus grunniens).The results showed that the length of ...Primers based upon the mitochondrial genome sequences of Bos taurus were used to amplify and sequence the complete mitochondrial D-loop region of Jiulong yak(Poephagus grunniens).The results showed that the length of D-loop was 893 bp,with 87.4%homology to the Bos taurus D-loop sequence;there were 17 bp deletion.Using Ovis aries as an outgroup,the phylogeny of representative species of Bovinae(P.grunniens,P.mutus,Bos taurus,Bos indicus,Bison bison,Bison bonasus,and Bubalus bubalis)was analyzed.Among Bovinae,the sequence divergence between P.grunniens,P.mutus and American bison(Bison bison)was 6.2%-6.8%,which was less than that of Bos taurus and Bos indicus within Bos(10.0%-11.3%).Phylogenetic analysis found that P.grunniens,P.mutus and Bison bison clustered first of all,indicating there was higher genetic comparability among them than to that of Bos.Combining data from paleontology,morphology,and molecular biology,the present analysis supports the argument that Poephagus grunniens and Poephagus mutus should be classified as a distinct genus in Bovinae,that is Poephagus.展开更多
Selecting 6 heads of new yak strains(the offspring of reciprocal cross of F1 produced by wild yak male mated with domestic yak female)and 6 domestic yaks at 3 and 12 age in months respectively at Datong Yak Farm of Qi...Selecting 6 heads of new yak strains(the offspring of reciprocal cross of F1 produced by wild yak male mated with domestic yak female)and 6 domestic yaks at 3 and 12 age in months respectively at Datong Yak Farm of Qinghai Province. This paper was studied for the morphological characteristics of the skin and hair coat of yak and their adaptability to the cold living environment in Qinghai-Tibetan plateau. The results indicated that it should be of vital importance marker for the morphological characteristics of the skin and hair coat with two type of yaks at 3 and 12 age in months and their regulations of growth and development to adapt to the cold living environment in Qinghai-Tibetan plateau,The main morphological indexes of the skin and hair coat of the rearing new yak strain were higher than those of the domestic yaks at the same age in months, or speak precisely, the new yak strain was more powerful adaptable to the alpine cold living environment than that of the domestic yaks. The resvlts above provided scientific basis for the native strain breeding of yak and the new yak strain rearing.展开更多
文摘为了分析甘南牦牛(Bos grunniens)肌肉萎缩盒蛋白32(F-box protein 32,FBXO32)基因的单核苷酸多态性(single nucleotide polymorphism,SNP)位点,以及基因型与胴体和肉质性状间的相关性,本研究以593头甘南牦牛为研究对象,采用混池测序和竞争性等位基因特异性PCR(kompetitive allele specific PCR,KASP)技术,检测了甘南牦牛FBXO32基因突变位点及基因型,分析了基因型与甘南牦牛胴体及肉质性状的相关性。结果表明,从甘南牦牛FBXO32基因检测到7个SNP位点,分别是位于5′UTR区的SNP1(g.267A>C)、外显子1区的SNP2(g.326G>T)、外显子8区的SNP3(g.31231G>C),以及3′UTR区的SNP4(g.31352G>A)、SNP5(g.31424C>T)、SNP6(g.31503A>C)和SNP7(g.31504A>G)。其中,SNP1、SNP4、SNP5、SNP6与肌肉嫩度显著相关(P<0.05),SNP3、SNP6、SNP7与失水率显著相关(P<0.05),SNP1、SNP2、SNP3、SNP4、SNP5、SNP7与眼肌面积显著相关(P<0.05),SNP2、SNP5、SNP6与胴体重显著相关(P<0.05)。7个SNP位点构建的9种有效单倍型组合中,单倍型组合H2H2的个体肌肉嫩度小、熟肉率高,单倍型组合H1H2的个体熟肉率高、眼肌面积大、失水率低。FBXO32基因突变位点可作为甘南牦牛胴体及肉质性状潜在分子遗传标记,研究结果丰富了甘南牦牛经济性状分子遗传理论基础。
文摘建立了毛细管电泳(CE)测定乳中乳清酸含量的方法。用三氯乙酸提取昌台牦牛(Bos grunniens)和两种品牌液态乳脱脂乳中的乳清酸,然后用毛细管电泳进行分离和定量。对试验条件(缓冲液浓度、p H等)进行了优化,乳清酸色谱保留时间为15 min左右,最小检测限为0.02 mg/100 m L,在检测范围内线性关系良好(R2=0.999),回收率为99.66%。试验共测定了12头处于泌乳中期的昌台牦牛乳中的乳清酸含量,平均含量为(5.2±2.4)mg/100 m L,个体间含量差异较大。两种商业品牌的液态乳中乳清酸的含量平均为7.4 mg/100 m L,其中冷藏乳中乳清酸含量均高于常温乳,牦牛乳中的乳清酸含量低于商业液态乳。
文摘Primers based upon the mitochondrial genome sequences of Bos taurus were used to amplify and sequence the complete mitochondrial D-loop region of Jiulong yak(Poephagus grunniens).The results showed that the length of D-loop was 893 bp,with 87.4%homology to the Bos taurus D-loop sequence;there were 17 bp deletion.Using Ovis aries as an outgroup,the phylogeny of representative species of Bovinae(P.grunniens,P.mutus,Bos taurus,Bos indicus,Bison bison,Bison bonasus,and Bubalus bubalis)was analyzed.Among Bovinae,the sequence divergence between P.grunniens,P.mutus and American bison(Bison bison)was 6.2%-6.8%,which was less than that of Bos taurus and Bos indicus within Bos(10.0%-11.3%).Phylogenetic analysis found that P.grunniens,P.mutus and Bison bison clustered first of all,indicating there was higher genetic comparability among them than to that of Bos.Combining data from paleontology,morphology,and molecular biology,the present analysis supports the argument that Poephagus grunniens and Poephagus mutus should be classified as a distinct genus in Bovinae,that is Poephagus.
文摘Selecting 6 heads of new yak strains(the offspring of reciprocal cross of F1 produced by wild yak male mated with domestic yak female)and 6 domestic yaks at 3 and 12 age in months respectively at Datong Yak Farm of Qinghai Province. This paper was studied for the morphological characteristics of the skin and hair coat of yak and their adaptability to the cold living environment in Qinghai-Tibetan plateau. The results indicated that it should be of vital importance marker for the morphological characteristics of the skin and hair coat with two type of yaks at 3 and 12 age in months and their regulations of growth and development to adapt to the cold living environment in Qinghai-Tibetan plateau,The main morphological indexes of the skin and hair coat of the rearing new yak strain were higher than those of the domestic yaks at the same age in months, or speak precisely, the new yak strain was more powerful adaptable to the alpine cold living environment than that of the domestic yaks. The resvlts above provided scientific basis for the native strain breeding of yak and the new yak strain rearing.
