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超深渊钩虾Eurythenes gryllus i型溶菌酶的基因克隆与表达 被引量:2
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作者 李雪雪 阮灵伟 《应用海洋学学报》 CSCD 北大核心 2020年第1期42-48,共7页
根据研究证明,在机体先天免疫系统中溶菌酶作为重要的效应分子之一,通过参与机体中多种免疫反应,主要是通过形成一个水解体系,从而破坏和消除侵害体内的病原体,进而促使机体实现免疫防御。本研究以生活在深海超深渊海沟极端环境下的钩虾... 根据研究证明,在机体先天免疫系统中溶菌酶作为重要的效应分子之一,通过参与机体中多种免疫反应,主要是通过形成一个水解体系,从而破坏和消除侵害体内的病原体,进而促使机体实现免疫防御。本研究以生活在深海超深渊海沟极端环境下的钩虾Eurythenes gryllus为材料,首次从Eurythenes gryllus中克隆获得i型溶菌酶(i-type lysozyme)基因EgLyz。序列分析表明,基因EgLyz与已知序列相似性较低,并含有一个失稳酶结构域。综合分析结果表明EgLyz可能是一个新颖的溶菌酶蛋白。在此基础上,对基因EgLyz进行了大肠杆菌原核重组表达,成功获取了较高纯度的可溶性表达产物,为后续生物活性分析奠定了实验基础。 展开更多
关键词 海洋生物学 溶菌酶 Eurythenes gryllus 超深渊 克隆表达
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GRYLLUS法稀汁脱钙—海伦糖厂引进技术之一
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作者 王本桂 《轻工设计(长沙)》 1989年第1期3-6,共4页
关键词 制糖 gryllus 脱钙 引进技术
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Cricket Farming for Animal Protein as Profitable Business for Small Farmers in Indonesia
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作者 Asnath Maria Fuah Hotnida Caroline Herawati Siregar Yuni Cahya Endrawati 《Journal of Agricultural Science and Technology(A)》 2015年第4期296-304,共9页
Cricket is widely recognized in Indonesia especially in Java island, and commonly kept by village farmers for food and feed. In general, crickets has very short life cycle (60-70 d), with three weeks of laying perio... Cricket is widely recognized in Indonesia especially in Java island, and commonly kept by village farmers for food and feed. In general, crickets has very short life cycle (60-70 d), with three weeks of laying period and egg production within the range of 200-1,500 eggs/female. The female has capability for multiple mating with several males and therefore produces more fertile eggs. Serial studies including experiments on laboratory and field observation of local crickets had been conducted to evaluate the farming practices and reproductive performance of three local species (Gryllus spp., Gryllus mitratus and Gryllus testaceus) which were given local feeds--Manihot esculenta and Carica papaya leaves. The experiment was conducted in randomized block design with feed as the treatments and species as the block. Cliring crickets (G. mitratus) fed with M. esculenta leaves revealed the highest total egg production (1,478.22 eggs), with daily egg production of 32.85 eggs/d, the longest laying period (45 d), the lowest feed intake (0.28 g/head) and feed conversion (0.93 mg/egg), compared to Cendawang crickets (G. testaceus) and Gryllus spp.. The revenue and cost (R/C) ratio was 1.4, indicating that small scale cricket enterprise could offer significant profit and could be chosen as an alternative activities for earning regular income. 展开更多
关键词 gryllus local feed performance economic potential.
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