An integrated expression vector pGT9GH was constructed by using guaC gene of receptor strain as guide sequence for homologous recombination.And then,by using the method of double crossover recombination between plasmi...An integrated expression vector pGT9GH was constructed by using guaC gene of receptor strain as guide sequence for homologous recombination.And then,by using the method of double crossover recombination between plasmid and chromosome,the linearized plasmid pGT9GH were integrated into B.subtilis GJ06 to get B.subtilis GJ07 with guaC gene mutant,in which,one copy of riboflavin operon was inserted between 5' and 3' end of guaC gene.The homologous recombination events were confirmed by PCR methods,and then,the riboflavin yield of mutant strain GJ07 was measured.The results of shake flask culture showed that the production of riboflavin of GJ07 was 24.5% higher than that of GJ06 in 60 h.展开更多
基金Supported by National Key New Product Plan[2003ED760039]Research Starting Fund for Introduction(training)of Personnel inWuhan Polytechnic University[2010RZ16]~~
文摘An integrated expression vector pGT9GH was constructed by using guaC gene of receptor strain as guide sequence for homologous recombination.And then,by using the method of double crossover recombination between plasmid and chromosome,the linearized plasmid pGT9GH were integrated into B.subtilis GJ06 to get B.subtilis GJ07 with guaC gene mutant,in which,one copy of riboflavin operon was inserted between 5' and 3' end of guaC gene.The homologous recombination events were confirmed by PCR methods,and then,the riboflavin yield of mutant strain GJ07 was measured.The results of shake flask culture showed that the production of riboflavin of GJ07 was 24.5% higher than that of GJ06 in 60 h.
