A Preliminary Study on Conservation Genetics of Endangered Vatica guangxiensis (Dipterocarpaceae)

运用 2 0个 10碱基随机引物 ,对中国龙脑香科 (Dipterocarpaceae)特有的珍稀濒危植物版纳青梅 (VaticaguangxiensisX .L .Mo)进行了RAPD多态性分析。 3个自然居群和 1个迁地保护居群 (分布于云南和广西 )共扩增出2 31个位点 ,多态位点所占比例 (PPB)为 5 3.6 8% ;观察等位基因数na =1.5 36 8,有效等位基因数ne =1.2 878,Nei基因多样性指数h为 0 .16 86 ,居群内的遗传多样性水平较低。基于AMOVA和POPGENE的结果均表明居群内的遗传变异大于居群间的遗传变异。居群内的遗传变异为 5 5 .0 9% ,居群间的变异为 44 .91% (AMOVA) ;基因分化系数Gst为 0 .3746 (POPGENE) ,表明居群间存在高水平的遗传分化。研究结果对该濒危植物的保护有重要意义。考虑到低水平的遗传多样性和高水平的居群分化 ,通过居群间种子和幼苗的交换来促进基因流是可行的保护方案。迁地保护居群 (ML)不具最高的遗传多样性 ,表明为了保护此濒危物种的全部遗传变异 。

Reproductive Biology of Boiga guangxiensis Wen,1998(Serpentes:Colubridae)

Up to now, the reproductive biology of most species of the genus Boiga is investigated very little. Important data on the reproductive biology of Boiga guangxiensis were received by us for the first time. The investigations were carried out in Viet Nam during expeditions and in the laboratories of Tula Exotarium in the period between 1998-2000. The data on the feeding, egg size, incubation, size of hatchings, and juvenile colouration of this species are provided for the first time. In addition, the data on size and ratio of snout-vent length/tail length of this snake are provided based on the Vietnamese samples. Our researches demonstrated that B. guangxiensis is distinguished from most other species of the genus by a number of peculiarities of its reproductive biology. Also, morphological differences between Chinese and Vietnamese individuals of this species were noted.

基于正交试验的2种广西特有秋海棠扦插繁殖

研究以广西秋海棠(Begonia guangxiensis)和橙花侧膜秋海棠(B.aurantiflora)的健康成熟叶片为繁殖材料,以珍珠岩、蛭石和河沙为扦插基质,以3-吲哚乙酸(IAA)和萘乙酸(NAA)为生长调节剂,采用L9(3~3)正交试验设计,探讨3种生长调节剂(IAA、NAA、IAA和NAA等量混合)及3种不同处理浓度下(100、200和300 mg/L)对橙花侧膜秋海棠和广西秋海棠叶片扦插的成活率、出苗率和生根效果的影响。结果表明,扦插基质对橙花侧膜秋海棠和广西秋海棠的生根效果有显著影响(P<0.05),而其他因素对2种秋海棠的成活率、出苗率和生根效果无显著影响(P>0.05)。根据极差分析结果,橙花侧膜秋海棠叶片最优扦插组合为:浓度为200 mg/L的IAA激素+蛭石基质;广西秋海棠叶片最优扦插组合为:浓度为100 mg/L的IAA和NAA混合溶液+河沙基质。该结果可为橙花侧膜秋海棠和广西秋海棠叶片扦插快繁提供参考。