The pursuit of high oil recovery rate has been a persistent objective for oil industry. Pseudomonas sp. LP-7 and Bacillus sp. PAH-2 were isolated from oil-contaminated surface soil samples of an oilfield. The antimicr...The pursuit of high oil recovery rate has been a persistent objective for oil industry. Pseudomonas sp. LP-7 and Bacillus sp. PAH-2 were isolated from oil-contaminated surface soil samples of an oilfield. The antimicrobial degradation rates(ADRs) of polymers achieved by LP-7 and PAH-2 were evaluated at a temperature of 35 °C in the mineral salt media during the shaken flask trial. The ADRs of copolymer synthesized by using a surfactant with a concentration of 5% could reach 8.4% for PAH-2 and 15.3% for LP-7. The ADRs of copolymer could reach 10.4% for PAH-2 and 21.3% for LP-7,when the polymer concentration was 2 g/L. All results confirmed that the ADRs of copolymers increased with an increasing content of HDDE(capsaicin derivative monomer) in the polymer. The copolymers also manifested excellent antimicrobial degradation performance in the presence of Cu^(2+), Zn^(2+), and Pb^(2+) ions, respectively, which had great potential for applications in enhanced oil recovery.展开更多
The massive use of antibiotics in aquaculture and resulted antibacterial resistance problem urgently need antibiotic substitutions. Herein, we report a promising substitution of aquaculture antibiotics using a synergi...The massive use of antibiotics in aquaculture and resulted antibacterial resistance problem urgently need antibiotic substitutions. Herein, we report a promising substitution of aquaculture antibiotics using a synergistic combination of biodegradable peptide polymers and curcumin, a natural compound from plant. The synergistic combination shows strong antibacterial activity against V. fluvialis and some other common bacteria in aquaculture. The membrane-damaging antibacterial mechanism echoes our finding that the synergistic combination will not induce bacteria to develop resistance after continuous use. The synergistic combination also displays effective cure on V. fluvialis-infected zebrafish. The biodegradability of the peptide polymer enables the combination to lose antibacterial activity and will not cause selective pressure on bacterial in the environment. Our study indicates potential application of synergistic composition, biodegradable peptide polymer and curcumin, as promising antibiotic substitution in aquaculture, which represents a promising strategy to address the global challenge of antimicrobial resistance.展开更多
Antimicrobial resistance(AMR)is one of the major challenges in pathogenic infections,which endangers human health and brings devastating public health threats.Therefore,developing new materials to reduce the over-reli...Antimicrobial resistance(AMR)is one of the major challenges in pathogenic infections,which endangers human health and brings devastating public health threats.Therefore,developing new materials to reduce the over-reliance on antibiotics or to replace antibiotics is in great need to solve this problem.Conjugated polymers(CPs)with excellent light-harvesting properties and good biocompatibility are ideal candidate materials for combat-ting AMR through photodynamic antimicrobial therapy(PACT)by sensitizing oxygen and photothermal therapy(PTT)by light to heat conversion.Sig-nificant works of CPs-based antimicrobial approaches have been reported in recent years.Herein,we summarize the types of CPs-based antimicrobial materials,illustrate their antimicrobial mechanisms,and discuss their applications in combating AMR mainly by PACT or antimicrobial PTT.In addi-tion,we proposed some other antimicrobial treatment strategies based on CPs,hoping to provide inspiring insight for overcoming AMR.展开更多
The emergence of antimicrobial resistance attributed to the overuse and abuse of antibiotics severely endangers global biosafety.Antimicrobial peptides(AMPs)produced by various living organisms exhibit broad-spectrum ...The emergence of antimicrobial resistance attributed to the overuse and abuse of antibiotics severely endangers global biosafety.Antimicrobial peptides(AMPs)produced by various living organisms exhibit broad-spectrum antimicrobial properties with a low propensity to the resistance.However,the application of AMPs has been greatly limited owing to their poor stability,high manufacturing cost,and high cytotoxicity.Thus,AMP-mimetic antimicrobial cationic polymers with cationic and amphiphilic moieties have attracted considerable attention as antimicrobial agents.These polymers typically exhibit broad-spectrum antimicrobial activities,negligible antimicrobial resistance,and rapid bactericidal effect.These polymers exhibit low hemolysis and cytotoxicity by optimizing their chemical structures.In this study,we summarize the design principles and current findings of antimicrobial cationic polymers and identify potential candidates for developing innovative polymeric antimicrobials.展开更多
The intensive use of antibiotics intensifies the development of bacterial resistance,which has become a serious problem globally.Methicillin-resistant Staphylococcus aureus(MRSA)has resulted in significant morbidity a...The intensive use of antibiotics intensifies the development of bacterial resistance,which has become a serious problem globally.Methicillin-resistant Staphylococcus aureus(MRSA)has resulted in significant morbidity and mortality.Therefore,it is an urgent need to develop new antimicrobial drugs and administration methods.Herein,we report a dual functional diblock copolymer PLL20-b-PBLG20,which was prepared by superfast and water-insensitive polymerization on N-carboxyanhydrides(NCA)initiated by tetraalkylammonium carboxylate.In addition to direct antimicrobial activity,PLL20-b-PBLG20 also exerts a synergistic bactericidal effect against MRSA with curcumin,a plant extract with antibacterial property.Moreover,PLL20-b-PBLG20 successfully encapsulates curcumin to form nanoparticles via self-assembly.The combination of dual functional PLL20-b-PBLG20 and curcumin holds promise in combating MRSA infections.展开更多
基金the Natural Science Foundation of China(50673085,41576077)the National High-Tech Research and Development Programme of China(2010AA09Z203)the Fundamental Research Funds for the Central Universities of China(201562026)
文摘The pursuit of high oil recovery rate has been a persistent objective for oil industry. Pseudomonas sp. LP-7 and Bacillus sp. PAH-2 were isolated from oil-contaminated surface soil samples of an oilfield. The antimicrobial degradation rates(ADRs) of polymers achieved by LP-7 and PAH-2 were evaluated at a temperature of 35 °C in the mineral salt media during the shaken flask trial. The ADRs of copolymer synthesized by using a surfactant with a concentration of 5% could reach 8.4% for PAH-2 and 15.3% for LP-7. The ADRs of copolymer could reach 10.4% for PAH-2 and 21.3% for LP-7,when the polymer concentration was 2 g/L. All results confirmed that the ADRs of copolymers increased with an increasing content of HDDE(capsaicin derivative monomer) in the polymer. The copolymers also manifested excellent antimicrobial degradation performance in the presence of Cu^(2+), Zn^(2+), and Pb^(2+) ions, respectively, which had great potential for applications in enhanced oil recovery.
基金supported by the National Natural Science Foundation of China(Nos.22075078,21861162010)the Program of Shanghai Academic/Technology Research Leader(20XD1421400)+1 种基金Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism(Shanghai Municipal Education Commission)the Fundamental Research Funds for the Central Universities(JKD01211520).
文摘The massive use of antibiotics in aquaculture and resulted antibacterial resistance problem urgently need antibiotic substitutions. Herein, we report a promising substitution of aquaculture antibiotics using a synergistic combination of biodegradable peptide polymers and curcumin, a natural compound from plant. The synergistic combination shows strong antibacterial activity against V. fluvialis and some other common bacteria in aquaculture. The membrane-damaging antibacterial mechanism echoes our finding that the synergistic combination will not induce bacteria to develop resistance after continuous use. The synergistic combination also displays effective cure on V. fluvialis-infected zebrafish. The biodegradability of the peptide polymer enables the combination to lose antibacterial activity and will not cause selective pressure on bacterial in the environment. Our study indicates potential application of synergistic composition, biodegradable peptide polymer and curcumin, as promising antibiotic substitution in aquaculture, which represents a promising strategy to address the global challenge of antimicrobial resistance.
基金The authors are grateful for the financial support of the National Natural Science Foundation of China(Nos 21773054,21905072,and 22077025)the Natural Science Foundation of Hebei Province(Nos.B2020202062,B2020202086)。
文摘Antimicrobial resistance(AMR)is one of the major challenges in pathogenic infections,which endangers human health and brings devastating public health threats.Therefore,developing new materials to reduce the over-reliance on antibiotics or to replace antibiotics is in great need to solve this problem.Conjugated polymers(CPs)with excellent light-harvesting properties and good biocompatibility are ideal candidate materials for combat-ting AMR through photodynamic antimicrobial therapy(PACT)by sensitizing oxygen and photothermal therapy(PTT)by light to heat conversion.Sig-nificant works of CPs-based antimicrobial approaches have been reported in recent years.Herein,we summarize the types of CPs-based antimicrobial materials,illustrate their antimicrobial mechanisms,and discuss their applications in combating AMR mainly by PACT or antimicrobial PTT.In addi-tion,we proposed some other antimicrobial treatment strategies based on CPs,hoping to provide inspiring insight for overcoming AMR.
基金This work was supported by National Natural Science Foundation of China(Nos.52073153 and 51722302)Natural Science Foundation of Shandong Province(No.ZR2019JQ17).
文摘The emergence of antimicrobial resistance attributed to the overuse and abuse of antibiotics severely endangers global biosafety.Antimicrobial peptides(AMPs)produced by various living organisms exhibit broad-spectrum antimicrobial properties with a low propensity to the resistance.However,the application of AMPs has been greatly limited owing to their poor stability,high manufacturing cost,and high cytotoxicity.Thus,AMP-mimetic antimicrobial cationic polymers with cationic and amphiphilic moieties have attracted considerable attention as antimicrobial agents.These polymers typically exhibit broad-spectrum antimicrobial activities,negligible antimicrobial resistance,and rapid bactericidal effect.These polymers exhibit low hemolysis and cytotoxicity by optimizing their chemical structures.In this study,we summarize the design principles and current findings of antimicrobial cationic polymers and identify potential candidates for developing innovative polymeric antimicrobials.
基金supported by the National Key Research and Development Program of China(2022YFC2303100)the National Natural Science Foundation of China(Nos.52203162,22075078)+5 种基金Program of Shanghai Academic/Technology Research Leader(20xD1421400)China National Postdoctoral Program for Innovative Talents(Bx20220108)the China Postdoctoral Science Foundation(2021M701198)Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry(Changchun Institute of Applied Chemistry,Chinese Academy of Sciences),the Open Project of Engineering Research Center of Dairy Quality and Safety Control Technology(Ministry of Education,R202201)Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism(Shanghai Municipal Education Commission)Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization in this manuscript.
文摘The intensive use of antibiotics intensifies the development of bacterial resistance,which has become a serious problem globally.Methicillin-resistant Staphylococcus aureus(MRSA)has resulted in significant morbidity and mortality.Therefore,it is an urgent need to develop new antimicrobial drugs and administration methods.Herein,we report a dual functional diblock copolymer PLL20-b-PBLG20,which was prepared by superfast and water-insensitive polymerization on N-carboxyanhydrides(NCA)initiated by tetraalkylammonium carboxylate.In addition to direct antimicrobial activity,PLL20-b-PBLG20 also exerts a synergistic bactericidal effect against MRSA with curcumin,a plant extract with antibacterial property.Moreover,PLL20-b-PBLG20 successfully encapsulates curcumin to form nanoparticles via self-assembly.The combination of dual functional PLL20-b-PBLG20 and curcumin holds promise in combating MRSA infections.
