Effects of preventive acupuncture or moxibustion at the Guanyuan acupoint on expression of interleukin-2 and its receptors in hypothalamus, serum, and uterus of menopausal rats

BACKGROUND： Previous studies have indicated preventive acupuncture and moxibustion at the Guanyuan point have positive effects on the immune system of menopausal rats. OBJECTIVE： To observe the effects of preventive acupuncture or moxibustion at the Guanyuan point on interleukin-2 （IL-2） and its receptor （IL-2R mRNA） levels in hypothalamus, serum, and uterus of menopausal rats. DESIGN： Randomized control animal experiment. SETTING： School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine. MATERIALS： This study was performed at the Laboratory of Beijing University of Chinese Medicine from November 2004 to July 2005. The vaginal smear method was used to determine the estrus cycle of 9.5-month-old Sprague Dawley （SD） female rats. Three continuous estrus cycles were measured, and 160 rats with estrus cycle disorder, weighing （300 _＋ 20） g, were included in this study. The rats were randomly divided into four groups： preventive acupuncture group （n =48）, preventive moxibustion group （n =48）, model groups （n =48）, and 10-month-old group （n =16）. Sixteen 3.5-month-old healthy SD female rats, weighing （240 ± 20） g, were also assigned to the young group. All rats were clean grade and were purchased from the Laboratory Animal Center, Beijing University of Chinese Medicine. The experiment was in accordance with animal ethics standards. METHODS： Acupuncture was applied to the preventive acupuncture group at 10 months of age. The needle was inserted upward and perpendicularly two fen to a point that was two fen below the Guanyuan point. The rats freely moved around during needle retention （20 minutes）. Acupuncture was administered twice a week and continued for eight weeks. A moxa cone （small） moxibustion was applied to the preventive moxibustion group at 10 months of age. The rats were caught gently and kept at the same position as the preventive acupuncture group. The moxa cone was ignited and held to the Guanyuan point directly. Only one moxa cone was used each time. After moxibustion, the local skin turned slightly red. Moxibustion was used twice a week and continued for eight weeks. Nothing except gently catching and lying was administered to the model group and the young group. MAIN OUTCOME MEASURES： Radioimmunoassay, in situ hybridization, and similar methods were employed to detect IL-2 in serum and the uterus, as well as IL-2R mRNA expression in the hypothalamus and uterus arcuate nuclei, of the preventive acupuncture group, preventive moxibustion group, model groups （at 12, 14, and 16 months）, 10 month old group （at 10 months）, and the young group （at 4 months）. RESULTS： All 176 rats were included in the final analysis. （1） IL-2R mRNA： IL-2R mRNA expression decreased significantly in the uterus of 12-, 14-, and 16-month-old model groups and in the hypothalamus of 14- and 16-month-old model groups （P 〈 0.05-0.01）. Compared to the same month-aged model groups, the expression of IL-2R mRNA increased significantly in the hypothalamus and uterus of 16-month-old preventive acupuncture or moxibustion groups （P 〈 0.05-0.01）.（2） IL-2：IL-2 in serum of 12- and 14-month-old model groups and in the uterus of 12-, 14-, and 16-month-old model groups decreased significantly compared to the young group （P 〈 0.05-0.01）. IL-2 serum levels increased significantly in the 16-month-old group with preventive moxibustion; in addition, IL-2 expression levels increased significantly in the uterus of the 12-month-old group with preventive moxibustion, as well as the 12- and 16-month-old groups with preventive acupuncture （P 〈 0.05-0.01）. CONCLUSION： As a rat ages and becomes menopausal, IL-2R mRNA expression in the hypothalamus and uterus, as well as IL-2 levels in the serum and uterus, decrease. However, preventive acupuncture and preventive moxibustion can repress the decrease to some extent. This effect is even greater in the late stages of menopause （16-month-old rats）.

Effect of wheat maxibastion of Guanyuan point on immune function and antitumor effect in mice with tumor

Objective To investigate wheat maxibastion of Guanyuan point on immune function and antitumor effect in mice with tumor.Method Group experiment and statistical analysis were conducted.Results were analyzed comparatively.Result Wheat maxibastion of Guanyuan point could reduce ATEP(associated tumor erythrocyte rate) and direct tumor erythrocyte rate(DTER) and raise excitotumorous erythrocyte rate(ETER)(P< 0.05,P< 0.01) and stimulate the reduced activity of NK cells and IL 2 significantly(P< 0.01) in mice with tumor.Conclusion Wheat maxibastion of Guanyuan point can increase the adhesion ability to tumor cells of RBC,and increase the activity of NK cell and IL 2.

关元命门序贯针刺激活FSHR/cAMP/PKA通路促进早发性卵巢功能不全模型大鼠颗粒细胞增殖的机制研究

【目的】观察关元命门序贯针刺方案对早发性卵巢功能不全(POI)模型大鼠的治疗作用及机制。【方法】将雌性SD大鼠分为空白组、模型组、蛋白激酶A(PKA)抑制剂(H89)+针刺组、针刺组各12只。除空白组,其他3组大鼠采用雷公藤多苷片灌胃制备POI模型。模型成功建立后,空白组和模型组每日捆绑一次;针刺组大鼠在动情间期取关元穴针刺,在动情前期取命门穴针刺;H89+针刺组按照针刺组针刺方案干预,在每次针刺前30 min内腹腔注射H89。连续干预20 d。各组大鼠分别在干预后第1个动情间期和动情前期取材。酶联免疫吸附分析(ELISA)检测动情间期促卵泡激素(FSH)、雌二醇(E2)水平,Western Blot法检测动情间期促卵泡激素受体(FSHR)、芳香化酶P450(P450arom)蛋白表达,细胞计数试剂盒8(CCK-8)法检测动情间期和动情前期颗粒细胞活性,免疫组织化学法检测动情前期增殖细胞核抗原(PCNA)蛋白表达水平。【结果】(1)与空白组比较,模型组和H89+针刺组血清FSH水平显著升高(P<0.01),E2水平显著降低(P<0.001);H89+针刺组FSH水平与模型组无差异(P>0.05),E2水平低于模型组(P<0.05);针刺组FSH水平低于模型组和H89+针刺组(P<0.05),与空白组无差异(P>0.05),E2水平显著高于模型组和H89+针刺组(P<0.01),仍低于空白组(P<0.05)。(2)模型组和H89+针刺组FSHR、P450arom蛋白表达均低于空白组(P<0.01);H89+针刺组FSHR蛋白表达水平与模型组无差异(P>0.05),P450arom蛋白表达水平低于模型组(P<0.05);针刺组FSHR、P450arom蛋白表达水平均高于模型组和H89+针刺组(P<0.05),但仍低于空白组(P<0.05)。(3)模型组和H89+针刺组GCs活性和PCNA平均光密度值均低于空白组(P<0.05);H89+针刺组GCs活性和PCNA平均光密度值均低于模型组(P<0.05);针刺组的GCs活性和PCNA平均光密度值显著高于模型组和H89+针刺组(P<0.05或P<0.01)。【结论】关元命门序贯针刺方案可通过上调促卵泡激素受体(FSHR)/环磷酸腺苷(cAMP)/蛋白激酶A(PKA)通路FSHR、P450arom蛋白的表达,调控性激素水平,提高GCs活性和促进GCs细胞增殖,从而改善POI。

Effects of electroacupuncture at Guanyuan(CV 4)or Sanyinjiao(SP 6)on hypothalamus-pituitary-ovary axis and spatial learning and memory in female SAMP8 mice

OBJECTIVE: To investigate the effects of electroacupuncture(EA) at the Guanyuan(CV 4) or Sanyinjiao(SP 6) acupoints on the hypothalamus-pituitary-ovary(HPO) axis and spatial learning and memory in female mice.METHODS: Nine-month-old female mice with senescence-accelerated mouse prone 8(SAMP8)were divided into three groups: the disease model,EA-Guanyuan and EA-Sanyinjiao groups. Concurrently, 9-month old female mice with senescence-accelerated mouse resistance 1(SAMR1)were set as the control model group. The two treatment groups were given the same pattern of EA stimulation. Gonadotropin-releasing hormone, luteinizing hormone, follicle-stimulating hormone(FSH) and Serum estradiol levels in the Hypothalamus-pituitary-ovary axis were assessed by enzyme-linked immunosorbent assay to determinethe HPO axis function level. Spatial learning and memory were assessed by the Morris Water Maze(MWM) test.RESULTS:(a) HPO axis: compared with the control model group, the disease model group displayed a decrease in E2 levels(P < 0.01), and an increase in Gn RH, LH and FSH levels(P < 0.01). E2 levels were increased in EA treatment groups compared with the disease model group(P < 0.05). In contrast,Gn RH and LH and FSH levels were reduced(P <0.05). EA-Sanyinjiao group was superior than EA-Guanyuan group on increasing E2 and declining Gn RH levels(P < 0.01).(b) The MWM test demonstrated that the response latency in the EA-Sanyinjiao treatment group declined from day 2 to day5 compared with the disease model group(P <0.05), whereas the EA-Guanyuan treatment group showed no significant difference.CONCLUSION: EA can regulate hormone(E2, FSH,LH, Gn RH) levels in the HPO axis and the spatial learning and memory ability in female SAMP8 mice. Moreover, this effect may have been more pronounced in the EA-Sanyinjiao group than the EA-Guanyuan group. The underlying mechanism of the EA-induced changes may be related to gonadal hormone shifts in the HPO axis, followed by an improvement in spatial learning and memory.

基于“气血和”探析关元穴延缓皮肤衰老的作用

气血作为人体生命活动的最基本物质,对人体各脏腑组织器官起着温煦、防御、气化及营养、滋润的作用。气血充足则筋骨强健,肌肉丰满,面色润泽,反应敏捷,气血失和则百病皆生。关元穴作为任脉腧穴,足三阴、阳明、任脉之会,可补气益血,调理冲任,保健强壮。当机体皮肤出现衰老现象时,从调和气血入手,使“气血和”以充分荣养、滋润于皮肤,令皮肤荣润细腻、富有光泽。

关元穴性特点在延缓衰老中的作用

关元穴能否通过补虚以延缓衰老,尚缺少考据与论证,故结合虚损致衰和病理产物致衰的病因病机,对古代医籍中关元穴的临床应用进行整理,总结出关元穴补中有通的穴性特点。关元穴穴名具有养生内涵,受道家思想影响,由位置关系而引申出保健延衰的功效设想,其培元固本认识的产生和演变具有时代背景。关元穴在隋唐以前即有近治作用的应用基础,从宋朝开始在临床中确立补虚培元、回阳救逆的功效,其延缓衰老的作用始在《扁鹊心书》得到应用和验证,而对于气滞、瘀血、痰湿等病理产物的通利作用,在后世中得到总结和运用。关元穴功效特点和衰老的病因病机能产生逻辑对应关系,应整体看待关元穴的作用,为研究关元穴延缓衰老的内在机制提供参考。

Effect of moxibustion at “Guanyuan”( CV4) and “Sanyinjiao”( SP 6) on bone morphology,metabolism and ERα of bone marrow mesenchymal stem cells in the ovariectomized rats

Objective To observe the effects of moxibustion at'Guanyuan'(CV 4) and'Sanyinjiao'(SP 6) on bone morphology,metabolism and ERαof bone marrow mesenchymal stem cells (MSCs) in the ovariectomized rats and explore the underlying mechanism of moxibustion at Guanyuan (CV 4) and Sanyinjiao (SP 6) on the regulationof bone metabolism. Methods A total of 60 SD ratswere randomized into a normal group (20 rats) and anovariectomy group (40 rats).

Effect of stimulating acupoint Guanyuan(CV 4) on lower back pain by burning moxa heat for different time lengths: a randomized controlled clinical trial

OBJECTIVE: To investigate the effect of different heat-stimulating time lengths on lower back pain.METHODS: Forty participants were randomly assigned to four groups of various heating time lengths. The short heating time length group(SL),moderate heating time length group(ML), and long heating time length group(LL) respectively received 15, 30, and 60 min of moxibustion therapy stimulating the acupoint Guanyuan(CV 4). The conventional acupuncture group(CA) received needle acupuncture treatment as a control group. The participants were treated continuously over a 2-week treatment period for a total of 10 sessions, with five sessions given per week. Participants were assessed weekly by blinded assessors, using the visual analogue scale(VAS) and Roland Morris Questionnaire(RMQ).RESULTS: The VAS and RMQ scores reduced in all four groups during treatment. There were significant differences in VAS scores(P < 0.01) and RMQ scores(P < 0.01) between before treatment and after 2 weeks of treatment in the LL group. After treatment, the LL group reported significantly lower VAS scores compared with the CA group, ML group,and SL group(P < 0.05).CONCLUSION: The long and moderate lengths of heat-stimulating time of 30 and 60 min may be more effective for relieving lower back pain than that of short stimulating time lengths.

Assessment of Heat-sensitization at Guanyuan （CV4） in Patients with Primary Dysmenorrhea： A Comparative Study between Moxibustion Sensation and Infrared Thermography

目的：采用灸感与红外法对原发性痛经患者关元穴热敏态进行检测对比研究，探讨腧穴热敏态红外客观显示的可能性。方法：共纳入原发性痛经患者71例，比较灸感法与红外法对原发性痛经患者关元穴热敏态的检测差异。结果：原发性痛经患者关元穴区发生热敏化时，其红外辐射强度多数显示低温特征，与灸感法比较，其敏感性（真阳性率）为76．6％，特异性（真阴性率）为70．1％，准确性为74．6％；艾灸热敏态关元穴区后，产生明显纵向或横向扩散的红外辐射增强区域，与灸感法比较，其敏感性（真阳性率）为78．7％，特异性（真阴性率）为83．3％，准确性为80.3％。结论：原发性痛经患者关元穴区热敏态在一定程度上可被红外成像客观显示；艾灸热敏腧穴产生的腧穴热敏现象（如扩热、传热），除了被受试者主观感觉之外，在一定程度上也能被红外成像客观显示。

Effect of a comprehensive therapy plus Gushenyutai plaster administered at Guanyuan(CV 4) on male infertility associated with semen non-liquefaction

OBJECTIVE: To observe the clinical effect of Gushenyutai plaster administered at the Guanyuan(CV4) acupoint on male infertility associated with semen non-liquefaction.METHODS: Sixty-two male patients with infertility caused by semen non-liquefaction were random-ized into a treatment and control group. The control group received comprehensive therapy, which included oral administration of clarithromycin sustained release tablets, hip bath with Zhongyaoxiaoyan granules, prostate massage, and transurethral microwave treatment. The treatment group was administered Gushenyutai plaster in addition to the comprehensive therapy. Both groups were treated for 8 weeks. After treatment, the clinical effect and pregnancy rate were assessed and compared between the two groups.RESULTS: The effective rate of the treatment group was significantly higher than that of the control group(96.77% vs 70.97%, P<0.05) and the pregnancy rates of the treatment group and control groups were 38.71% and 16.13%, respectively(P<0.05).CONCLUSION: The effect of Gushenyutai plaster plus comprehensive therapy was better than that of the comprehensive therapy alone on male infertility induced by semen non-liquefaction.

Effect of lit-moxa stimulation of Guanyuan(CV 4) acupoint on levels of lactic acid and super-oxide dismutase in skeletal muscle of rats

OBJECTIVE:To observe the effect of lit-moxa stimulating acupoint Guanyuan(CV 4) on lactic acid and super-oxide dismutase(SOD) in skeletal muscle after exercise exhaustion.METHODS:Twenty-eight adult male Sprague-Dawley rats were randomly divided into normal control group,exhausted control group,exercise group and moxibustion group using exercise training and mild heating with lit-moxa stick as treatment methods.The exhausted control group,moxibustion group and exercise group received an exhaustive swimming after 20 days of intervention.Swimming exhausted times were recorded.Lactic acid and SOD concentration in soleus muscle were detected and compared between every two groups.RESULTS:The swimming exhausted times of the moxibustion group and the exercise group were significantly increased compare to the exhausted control group(P < 0.05).The lactic acid of the exhausted control group was significantly increased comparing with the normal control group(P <0.05),and the lactic acid of the moxibustion group and the exercise group were significantly lower than that of the exhausted control group(P < 0.05).The SOD level of the exhausted control group was significantly decreased comparing with the normal control group(P < 0.05),and the SOD level of the moxibustion group and the exercise group were significantly higher than that of the exhausted control group(both,P < 0.05).There was no statistical difference between the moxibustion group and the exercise group.CONCLUSION:With lit moxa stick,heat stimulating acupoint of Guanyuan(CV 4) decreased the levels of lactic acid and SOD in rat's skeletal muscle.

Clinical Application of Moxibustion on Guanyuan(CV 4)

Guanyuan(CV 4)is located 3 cun below theumbilicus,on the midline of abdomen.It is theFront-Mu Point of the Small Intestine,a crossingpoint of Three Yin Meridians of Foot and ConceptionVessel.It can warm the kidney to invigorate yang,

电针关元穴经微RNA-199a-3p调控c-kit/ERK通路对神经源性尿潴留大鼠的治疗作用

目的探讨电针关元穴经miRNA-199a-3p调控酪氨酸激酶受体c-kit/ERK通路对神经源性尿潴留大鼠的治疗作用。方法采用改良脊髓横断法建立神经源性尿潴留大鼠模型60只,按随机数字表法平均分为5组:模型组、电针组、miRNA-199a-3p抑制剂组、miRNA-199a-3p抑制剂阴性对照组、电针+miRNA-199a-3p抑制剂组,另选取SD大鼠12只作为假手术组。使用药物分组干预处理后,检测大鼠尿动力学,比较各组大鼠膀胱最大容量、膀胱基础压、漏尿点压力;采用肌条实验检测大鼠逼尿肌兴奋性,比较各组肌条自发性收缩频率;采用H-E染色观察各组大鼠膀胱组织病理形态改变;用试剂盒测定各组大鼠血清炎症细胞因子环氧化酶2(COX-2)、IL-18、诱导型一氧化氮合酶(iNOS)水平;采用qPCR检测各组大鼠膀胱组织miRNA-199a-3p与c-kit mRNA表达水平;采用蛋白质印迹法检测各组大鼠膀胱组织c-kit/ERK通路蛋白表达水平。结果与假手术组相比,模型组大鼠膀胱组织呈现严重病理损伤改变,膀胱最大容量、膀胱基础压、漏尿点压力及血清COX-2、IL-18、iNOS水平均升高(P均<0.05),肌条自发性收缩频率、膀胱组织miRNA-199a-3p与c-kit mRNA表达水平、膀胱组织磷酸化(p)-ERK/ERK与c-kit表达水平均降低(P均<0.05)。与模型组、电针+miRNA-199a-3p抑制剂组相比,电针组大鼠膀胱组织病理损伤减轻,膀胱最大容量、膀胱基础压、漏尿点压力及血清COX-2、IL-18、iNOS水平均降低(P均<0.05),肌条自发性收缩频率、膀胱组织miRNA-199a-3p与c-kit mRNA表达水平、膀胱组织p-ERK/ERK与c-kit表达水平均升高(P均<0.05);miRNA-199a-3p抑制剂组大鼠膀胱组织病理损伤均加重,膀胱最大容量、膀胱基础压、漏尿点压力及血清COX-2、IL-18、iNOS水平均升高(P均<0.05),肌条自发性收缩频率、膀胱组织miRNA-199a-3p与c-kit mRNA表达水平及膀胱组织p-ERK/ERK与c-kit表达水平均降低(P均<0.05)。与模型组相比,miRNA-199a-3p抑制剂阴性对照组大鼠各指标水平差异无统计学意义(P均>0.05)。结论电针关元穴可通过上调miRNA-199a-3p表达激活c-kit/ERK通路,减轻神经源性尿潴留大鼠膀胱组织炎症损伤,增强逼尿肌兴奋性,修复膀胱功能,改善尿潴留症状。

隔药饼灸调控子宫内膜异位症小鼠磷脂酰肌醇3激酶信号通路的研究

目的:探讨隔药饼灸调控子宫内膜异位症(EMT)小鼠对子宫内膜磷脂酰肌醇3激酶(PI3K)信号通路表达的影响。方法:将42只6~8周龄雌性重度联合免疫缺陷病(SCID)小鼠按照随机区组分组法分为正常组(n=8)和造模组(n=34)。造模组小鼠均采用EMT自体移植法进行造模,按照随机数字表法选取2只小鼠验证造模成功后,按随机区组分组法分成EMT模型组、隔药饼灸组、西药组和艾灸加西药组4组,每组8只,并给予相应治疗。观察评估各组小鼠扭体反应及囊泡抑制率,采用蛋白质印迹法检测小鼠异位内膜组织中PI3K、蛋白激酶B(AKT)、哺乳动物雷帕霉素靶蛋白(mTOR)的表达。结果:与正常组比较,模型组、西药组、隔药饼灸组、艾灸加西药组小鼠的扭体反应次数升高,差异均有统计学意义(均P<0.01),与西药组比较,隔药饼灸组和艾灸加西药组扭体反应次数下降,差异均有统计学意义(均P<0.05);与模型组比较,西药组、隔药饼灸组、艾灸加西药组治疗前后异位子宫组织体积明显缩小,差异均有统计学意义(均P<0.05);与模型组异位内膜组织比较,西药组、隔药饼灸组、艾灸加西药组异位内膜组织中的PI3K、AKT、mTOR表达降低,差异均有统计学意义(均P<0.05);与隔药饼灸组比较,西药组、艾灸加西药组异位内膜组织中的PI3K、AKT、mTOR表达均升高,差异均有统计学意义(均P<0.05)。结论:隔药饼灸可能是通过下调小鼠子宫异位内膜组织PI3K/AKT/mTOR高表达而达到镇痛和抑制囊泡生长作用。

针灸调周法治疗肾虚血瘀型卵巢储备功能下降的临床观察

【目的】观察针灸调周法治疗肾虚血瘀型卵巢储备功能下降(DOR)的临床疗效。【方法】将120例肾虚血瘀型DOR患者随机分为对照组和治疗组,每组60例。对照组采用西药人工周期法治疗,从月经第5天起首先给予服用戊酸雌二醇片1 mg,每天1次,连续服用21 d,在服用戊酸雌二醇片的第11天给予加服黄体酮胶囊0.2 g,每天2次,连续服用10 d,连服3个月经周期;治疗组采用针灸调周法治疗,取穴:百会、关元、归来、中极、子宫穴、血海、三阴交、太溪、膈俞、肾俞、次髎,隔日1次,每周治疗3次,1个月经周期治疗12次,共治疗3个月经周期。观察2组患者治疗前后中医证候积分、基础性激素[卵泡刺激素(FSH)、黄体生成素(LH)、FSH/LH、雌二醇(E2)、抗缪勒管激素(AMH)]水平及窦卵泡数(AFC)的变化情况,并评价2组患者的中医证候疗效。【结果】(1)治疗3个月经周期后,治疗组的总有效率为93.33%(56/60),对照组为78.33%(47/60),组间比较,治疗组的中医证候疗效明显优于对照组(P<0.05)。(2)治疗后,2组患者的中医证候积分均较治疗前明显降低(P<0.01),且治疗组对中医证候积分的降低作用明显优于对照组(P<0.05)。(3)治疗后,2组患者的血清FSH、LH、FSH/LH水平均较治疗前降低(P<0.01),血清E2水平均较治疗前升高(P<0.01),且治疗组对血清FSH、LH、FSH/LH水平的降低作用及对血清E2水平的升高作用均明显优于对照组(P<0.05或P<0.01)。(4)治疗后,2组患者的血清AMH水平和AFC值均较治疗前升高(P<0.01),且治疗组对血清AMH水平和AFC值的升高作用均明显优于对照组(P<0.05或P<0.01)。【结论】针灸调周法治疗肾虚血瘀型DOR患者疗效确切,能有效改善患者的临床症状和卵巢储备功能,基于月经周期的针灸调周理念可为临床治疗DOR提供新的思路。

基于极值降雨假设法的城镇地质灾害风险性评价耦合模型研究——以安康市岚皋县官元镇为例

选取安康市岚皋县官元镇为研究区,以斜坡单元为评价单元,采用信息量-支持向量机耦合模型进行地质灾害易发性评价,根据自然断点法完成易发性分区;基于极值降雨假设法分别计算大雨(35 mm/24 h)、暴雨(75 mm/24 h)、大暴雨(175 mm/24 h)和特大暴雨(250 mm/24 h)4种工况下斜坡的危险性指数,并开展相应工况下危险性区划;采用TOPSIS法计算易损性指数,开展地质灾害易损性分区。综合采用信息量-支持向量机模型、极值降雨假设和TOPSIS法构建研究区风险性评价耦合模型,根据自然断点法划分4种降雨工况的地质灾害风险性分区,将风险区划为极高风险区、高风险区、中风险区和低风险区。

温和灸对原发性痛经模型大鼠子宫微循环及血液流变学的影响

目的:探讨温和灸对原发性痛经大鼠子宫微循环的影响。方法:将40只雌性SD大鼠随机分为空白组、空白加艾灸组、模型组、艾灸组,采用苯甲酸雌二醇联合缩宫素造模法制备大鼠子宫痛经模型,选择关元和三阴交对痛经大鼠进行温和灸治疗,分别观察测定大鼠扭体反应、子宫组织形态学、子宫微血管管径和血液流变学的变化情况。结果:除空白组和空白加艾灸组外,模型组与艾灸组均出现扭体反应,提示痛经大鼠模型制造成功;与空白组及空白加艾灸组比较,模型组子宫横径明显增加(P<0.05),微血管管径明显收缩(P<0.05)。与模型组比较,艾灸组子宫横径减少明显(P<0.05),微血管管径显著扩张(P<0.05);与空白组比较,模型组与艾灸组全血黏度各切变度、红细胞刚性指数、红细胞变形指数、红细胞沉降率方程K值及红细胞压积降低(P<0.05)。与模型组比较,艾灸组全血黏度各切变度、红细胞刚性指数、红细胞变形指数、红细胞沉降率方程K值及红细胞压积有升高的趋势(P>0.05);空白组与空白加艾灸组各项指标差异均无统计学意义(均P>0.05)。结论:温和灸对缩宫素所致大鼠实验性痛经有一定抑制作用,其抗痛经作用可能与改善子宫水肿及血液流变学,缓解子宫微循环障碍有关。

温针灸中极关元穴联合静脉滴注间苯三酚治疗产后尿潴留临床观察

目的探讨温针灸中极、关元穴联合静脉滴注间苯三酚在产后尿潴留中的临床疗效。方法选取2019年4月—2020年2月在江西省上栗县人民医院确诊的产后尿潴留患者60例,随机分为观察组和对照组,各30例。对照组采用常规方法治疗,观察组采用静脉滴注间苯三酚联合温针灸中极、关元穴治疗。比较2组患者的临床疗效首次排尿时间、膀胱残余尿量、导尿管留置率及泌尿系统感染情况。记录治疗期间所发生的不良反应。结果观察组治疗的总有效率为93.33%(28/30),高于对照组的73.33%(22/30)(P<0.05);观察组治疗后首次排尿时间、残余尿量、尿管留置率和泌尿系统感染率与对照组相比,差异有统计学意义(P<0.05);观察组治疗期间不良反应发生率为3.33%(1/30),与对照组的16.67%(5/30)相比,差异无统计学意义(P>0.05)。结论温针灸中极、关元穴联合静脉滴注间苯三酚治疗可以缩短产后尿潴留患者的首次排尿时间,减少膀胱残余尿量,降低导尿管留置率和泌尿系统感染率,效果显著。

《针灸大成》中对于关元穴的应用探析

关元穴为任脉腧穴,位于脐下3寸,募属小肠经,为脾肾肝经及任脉交会穴,具有调冲任、温气血、补下焦、固肾精等作用,临床应用十分广泛。本文就《针灸大成》中对于关元穴的应用进行论述。

芒针深刺关元俞配合真武汤加味治疗寒湿型单侧腰椎间盘突出症临床研究

目的:观察芒针深刺关元俞配合真武汤加味治疗寒湿型单侧腰椎间盘突出症的临床疗效。方法:选取2020年1月到2021年6月商丘市中医院针灸科治疗的寒湿型单侧腰椎间盘突出症患者100例作为研究对象,按照随机数字表法分为观察组和对照组,每组50例。对照组给予常规针刺配合真武汤加味治疗,观察组给予芒针深刺关元俞配合真武汤加味治疗。观察两组患者治疗前后视觉模拟评分(visual analogue score,VAS)、日本骨科学会制定的下腰痛评分(Japanese orthopaedic association scores,JOA)、抗人IgM免疫球蛋白、抗人IgG免疫球蛋白变化情况及临床疗效。结果:观察组有效率为96.00%,对照组有效率为86.00%,观察组有效率高于对照组,差异有统计学意义(P<0.05);观察组治疗后VAS评分、JOA评分低于对照组,差异有统计学意义(P<0.05);观察组不良反应发生率为4.00%,对照组不良反应发生率为2.00%,观察组不良反应发生率高于对照组,但差异无统计学意义(P>0.05);观察组治疗后抗人IgM免疫球蛋白、抗人IgG免疫球蛋白水平低于对照组,差异有统计学意义(P<0.05)。结论:芒针深刺关元俞配合真武汤加味治疗寒湿型单侧腰椎间盘突出症,能够提高临床疗效,减轻患者疼痛以及临床症状。