The application of microorganisms as probiotics is limited due to lack of safety evaluation.Here,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from ...The application of microorganisms as probiotics is limited due to lack of safety evaluation.Here,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove microorganisms.Its safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing analysis.Results showed that the genes and phenotypic expression of related virulence,antibiotic resistance and retroelement were rarely found.Hyphal morphogenesis genes(SIT4,HOG1,SPA2,ERK1,ICL1,CST20,HSP104,TPS1,and RHO1)and phospholipase secretion gene(VPS4)were annotated.True hyphae and phospholipase were absent.Only one retroelement(Tad1-65_BG)was found.Major biogenic amines(BAs)encoding genes were absent,except for spermidine synthase(JA9_002594),spermine synthase(JA9_004690),and tyrosine decarboxylase(inx).The production of single BAs and total BAs was far below the food-defined thresholds.GXDK6 had no resistance to common antifungal drugs.Virulence enzymes,such as gelatinase,DNase,hemolytic,lecithinase,and thrombin were absent.Acute toxicity test with mice demonstrated that GXDK6 is safe.GXDK6 has a good reproduction ability in the simulation gastrointestinal tract.GXDK6 also has a strong antioxidant ability,β-glucosidase,and inulinase activity.To sum up,GXDK6 is considered as a safe probiotic for human consumption and food fermentation.展开更多
This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the param...This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the parameters were studied under an experimental design 22 with triplicate at central point. The statistical analysis of the results indicated a significant negative effect on XR production from the variable inoculum. The variable dissolved oxygen also showed a negative effect on XR production. We found the maximum enzyme activity (2.5 U?mg?1) when both the factors were applied at their lowest levels. The yeast showed to be potentially capable for xylose reductase production when sugarcane bagasse hemicellulosic hydrolysate was used as carbon source. Also, the results presented important information for further optimization of xylose reductase attainment.展开更多
Background:Lymphatic filariasis(LF),a vector-borne parasitic disease,is endemic in several parts of India and mostly affects the poor or those with a low-income.The disease results in huge numbers of morbidities,disab...Background:Lymphatic filariasis(LF),a vector-borne parasitic disease,is endemic in several parts of India and mostly affects the poor or those with a low-income.The disease results in huge numbers of morbidities,disabilities,and deaths every year.Association of co-infection with other pathogens makes the condition more severe.Although co-infection is becoming a growing area of research,it is yet to emerge as a frontier research topic in filarial research specifically.This study reports the occurrence of a fungal infection in a large number of patients suffering from bancroftian filariasis in two districts of West Bengal,India.Methods:Nocturnal blood samples from filarial patients containing parasites and fungus were initially co-cultured,and further the fungus was isolated and characterized.Molecular identification of the isolate was carried out by PCR-based selective amplification and sequencing of highly-conserved D1/D2 region of 26S rDNA,whereas pathogenicity was determined by amplification of the RPS0 gene.A phylogenetic tree was constructed to study the relationship between the isolate and common pathogenic yeasts.The isolate was studied for antibiotic sensitivity,whereas morphological characterization was performed by microscopic techniques.Results:The isolate was identified as Pichia guilliermondii and this fungus was found to exist in co-infection with Wuchereria bancrofti in filarial patients.The fungus showed resistance to azole antifungals,griseofulvin,and,amphotericin B,whereas significant susceptibility was evident in cases of nystatin and cycloheximide.A total of 197 out of 222 patients showed this co-infection.Conclusion:This study revealed,for the first time,that P.guilliermondii exists as a co-infection in microfilaraemic individuals living in a filarial endemic zone.The findings are important and have relevance to human health,especially for filarial patients.展开更多
基金This research was supported by the Funding Project of Chinese Central Government Guiding to the Guangxi Local Science and Technology Development(GUIKEZY21195021)the Natural Science Fund for Distinguished Young Scholars of Guangxi Zhuang Autonomous Region of China(2019GXNSFFA245011)+3 种基金the Funding Project of Chinese Central Government Guiding to the Nanning Local Science and Technology Development(20231012)the Funding Projects of Guangxi Key Research and Development Plan(GUIKE AB23075173)the Funding Project of Technological Development from Angel Yeast(Chongzuo)Co.,Ltd.(JS1006020230722019)the Innovation Project of Guangxi Graduate Education(YCBZ2021012).
文摘The application of microorganisms as probiotics is limited due to lack of safety evaluation.Here,a novel multi-stress-tolerant yeast Meyerozyma guilliermondii GXDK6 with aroma-producing properties was identified from marine mangrove microorganisms.Its safety and probiotic properties were assessed in accordance with phenotype and whole-genome sequencing analysis.Results showed that the genes and phenotypic expression of related virulence,antibiotic resistance and retroelement were rarely found.Hyphal morphogenesis genes(SIT4,HOG1,SPA2,ERK1,ICL1,CST20,HSP104,TPS1,and RHO1)and phospholipase secretion gene(VPS4)were annotated.True hyphae and phospholipase were absent.Only one retroelement(Tad1-65_BG)was found.Major biogenic amines(BAs)encoding genes were absent,except for spermidine synthase(JA9_002594),spermine synthase(JA9_004690),and tyrosine decarboxylase(inx).The production of single BAs and total BAs was far below the food-defined thresholds.GXDK6 had no resistance to common antifungal drugs.Virulence enzymes,such as gelatinase,DNase,hemolytic,lecithinase,and thrombin were absent.Acute toxicity test with mice demonstrated that GXDK6 is safe.GXDK6 has a good reproduction ability in the simulation gastrointestinal tract.GXDK6 also has a strong antioxidant ability,β-glucosidase,and inulinase activity.To sum up,GXDK6 is considered as a safe probiotic for human consumption and food fermentation.
文摘This work evaluated the effect of dissolved oxygen and the initial inoculum concentration on xylose reductase (XR) production by Candida guilliermondii from sugarcane bagasse hemicellulosic hydrolysate. Both the parameters were studied under an experimental design 22 with triplicate at central point. The statistical analysis of the results indicated a significant negative effect on XR production from the variable inoculum. The variable dissolved oxygen also showed a negative effect on XR production. We found the maximum enzyme activity (2.5 U?mg?1) when both the factors were applied at their lowest levels. The yeast showed to be potentially capable for xylose reductase production when sugarcane bagasse hemicellulosic hydrolysate was used as carbon source. Also, the results presented important information for further optimization of xylose reductase attainment.
基金This study was funded by the Department of Biotechnology,Ministry of Science and Technology,(Grant No.BT/PR8779/Med/14/1282/2007)Council of Scientific and Industrial Research(Grant No.37(1516)/11/EMR-II),Govt.of IndiaSM,NM and PS thank University Grants Commission,Govt.of India for awarding their fellowships.
文摘Background:Lymphatic filariasis(LF),a vector-borne parasitic disease,is endemic in several parts of India and mostly affects the poor or those with a low-income.The disease results in huge numbers of morbidities,disabilities,and deaths every year.Association of co-infection with other pathogens makes the condition more severe.Although co-infection is becoming a growing area of research,it is yet to emerge as a frontier research topic in filarial research specifically.This study reports the occurrence of a fungal infection in a large number of patients suffering from bancroftian filariasis in two districts of West Bengal,India.Methods:Nocturnal blood samples from filarial patients containing parasites and fungus were initially co-cultured,and further the fungus was isolated and characterized.Molecular identification of the isolate was carried out by PCR-based selective amplification and sequencing of highly-conserved D1/D2 region of 26S rDNA,whereas pathogenicity was determined by amplification of the RPS0 gene.A phylogenetic tree was constructed to study the relationship between the isolate and common pathogenic yeasts.The isolate was studied for antibiotic sensitivity,whereas morphological characterization was performed by microscopic techniques.Results:The isolate was identified as Pichia guilliermondii and this fungus was found to exist in co-infection with Wuchereria bancrofti in filarial patients.The fungus showed resistance to azole antifungals,griseofulvin,and,amphotericin B,whereas significant susceptibility was evident in cases of nystatin and cycloheximide.A total of 197 out of 222 patients showed this co-infection.Conclusion:This study revealed,for the first time,that P.guilliermondii exists as a co-infection in microfilaraemic individuals living in a filarial endemic zone.The findings are important and have relevance to human health,especially for filarial patients.
