Objective:To purify and partially characterize the antimicrobial compounds from bacteriaBacillus sp.,isolated from fish gut.Methods:Protein and fatty acids were isolated from the bacteria and checked for the presence ...Objective:To purify and partially characterize the antimicrobial compounds from bacteriaBacillus sp.,isolated from fish gut.Methods:Protein and fatty acids were isolated from the bacteria and checked for the presence of antibacterial activity.Protein has been purified to apparent homogeneity from the supernatants of culture by means of ammonium sulphate precipitation followed by dialysis.Fourier transform infrared spectroscopy analyses were performed for proteins to identify the functional groups.Results:sulfate polyacrylamide gel electrophoresis.Fatty acids were extracted and subjected to gas chromatographic analysis.Conclusions:Protein showed an apparent molecular mass 56,47 and 39 kDa on sodium dodecyl acids and proteins which holds promise for the development of new drugs.The antimicrobial activity of the bacteria might be due to the presence of fatty acids and proteins which holds promise for the development of new drugs.展开更多
基金Supported by Department of science and technology with grant number-IF10573.
文摘Objective:To purify and partially characterize the antimicrobial compounds from bacteriaBacillus sp.,isolated from fish gut.Methods:Protein and fatty acids were isolated from the bacteria and checked for the presence of antibacterial activity.Protein has been purified to apparent homogeneity from the supernatants of culture by means of ammonium sulphate precipitation followed by dialysis.Fourier transform infrared spectroscopy analyses were performed for proteins to identify the functional groups.Results:sulfate polyacrylamide gel electrophoresis.Fatty acids were extracted and subjected to gas chromatographic analysis.Conclusions:Protein showed an apparent molecular mass 56,47 and 39 kDa on sodium dodecyl acids and proteins which holds promise for the development of new drugs.The antimicrobial activity of the bacteria might be due to the presence of fatty acids and proteins which holds promise for the development of new drugs.
