目的:了解各种临床类型乙型肝炎患者外周血细胞的促凝血活性(PCA)及新型促凝血因子人纤维介素(human fibrinogen like protein 2,hfgl2)的表达情况,及其与疾病进展的关系。方法:病毒性乙型肝炎慢性重型25例,健康对照25例,分离外周血单...目的:了解各种临床类型乙型肝炎患者外周血细胞的促凝血活性(PCA)及新型促凝血因子人纤维介素(human fibrinogen like protein 2,hfgl2)的表达情况,及其与疾病进展的关系。方法:病毒性乙型肝炎慢性重型25例,健康对照25例,分离外周血单个核细胞(PBMC);病毒性乙型肝炎慢性重度和重型患者标本33例,病毒性乙型肝炎慢性轻度、中度共25例,健康对照25例分离其外周血白细胞(WBC)。用一期冻融法测量其PCA。采用免疫组化的方法检测hfgl2的表达。结果:病毒性乙型肝炎慢性重型患者PBMC的PCA较健康对照高出20倍。WBC的PCA在以下各组(健康对照组、病毒性乙型肝炎慢性轻中度组、病毒性乙型肝炎慢性重度和重型组)呈逐步上升过程,但组间差异无显著性意义。PCA在病毒性乙型肝炎慢性重型组患者PBMC中的表达显著高于其在WBC的表达。PCA在健康对照组PBMC和WBC的表达无显著差异,hfgl2在病毒性乙型肝炎慢性重型患者PBMC中的表达水平显著高于其在健康对照组中的表达。结论:病毒性乙型肝炎慢性重型患者PBMC中的PCA和hfgl2表达均显著升高,且其表达与疾病严重程度相关,hfgl2表达的强度(阳性细胞数)与PCA的升高呈正比相关。展开更多
The aim of this study is to investigate the important regulative elements region which plays an important role on the activation of transcription exerted by the 5' noncoding region of hfgl2 gene in response to HBc...The aim of this study is to investigate the important regulative elements region which plays an important role on the activation of transcription exerted by the 5' noncoding region of hfgl2 gene in response to HBc and HBx. A series of promoter luciferase report plasmids, in which the hfgl2 gene has been deleted of the 5' and retained the common 3', were constructed. All the plasmids constructed were subjected to electrophoretic analysis and DNA sequencing. A eukaryotic construct expressing HBc or HBx, a luciferase reporter construct containing hfgl2 promoter and aβ-galactosidase (β-gal) plasmid were co-transfected into Chinese hamster ovary (CHO) cells and hepG2 cells, respectively. Luciferase report plasmids containing hfgl2 promoter were successfully constructed, and a serial assays of deletion of hfgl2 gene promoter showed that a strong regulatory region from -817 to -467 (relative to the transcription start site) was responsible for transcription and expression regulation of hfgl2 gene. The important regulative elements region in the promoter of hfgl2 gene was in response to HBc and HBx. which contributes to further pursuit of cis-acting elements and transcriptional factors involved in the transcription of hfgl2 gene.展开更多
文摘目的:了解各种临床类型乙型肝炎患者外周血细胞的促凝血活性(PCA)及新型促凝血因子人纤维介素(human fibrinogen like protein 2,hfgl2)的表达情况,及其与疾病进展的关系。方法:病毒性乙型肝炎慢性重型25例,健康对照25例,分离外周血单个核细胞(PBMC);病毒性乙型肝炎慢性重度和重型患者标本33例,病毒性乙型肝炎慢性轻度、中度共25例,健康对照25例分离其外周血白细胞(WBC)。用一期冻融法测量其PCA。采用免疫组化的方法检测hfgl2的表达。结果:病毒性乙型肝炎慢性重型患者PBMC的PCA较健康对照高出20倍。WBC的PCA在以下各组(健康对照组、病毒性乙型肝炎慢性轻中度组、病毒性乙型肝炎慢性重度和重型组)呈逐步上升过程,但组间差异无显著性意义。PCA在病毒性乙型肝炎慢性重型组患者PBMC中的表达显著高于其在WBC的表达。PCA在健康对照组PBMC和WBC的表达无显著差异,hfgl2在病毒性乙型肝炎慢性重型患者PBMC中的表达水平显著高于其在健康对照组中的表达。结论:病毒性乙型肝炎慢性重型患者PBMC中的PCA和hfgl2表达均显著升高,且其表达与疾病严重程度相关,hfgl2表达的强度(阳性细胞数)与PCA的升高呈正比相关。
文摘The aim of this study is to investigate the important regulative elements region which plays an important role on the activation of transcription exerted by the 5' noncoding region of hfgl2 gene in response to HBc and HBx. A series of promoter luciferase report plasmids, in which the hfgl2 gene has been deleted of the 5' and retained the common 3', were constructed. All the plasmids constructed were subjected to electrophoretic analysis and DNA sequencing. A eukaryotic construct expressing HBc or HBx, a luciferase reporter construct containing hfgl2 promoter and aβ-galactosidase (β-gal) plasmid were co-transfected into Chinese hamster ovary (CHO) cells and hepG2 cells, respectively. Luciferase report plasmids containing hfgl2 promoter were successfully constructed, and a serial assays of deletion of hfgl2 gene promoter showed that a strong regulatory region from -817 to -467 (relative to the transcription start site) was responsible for transcription and expression regulation of hfgl2 gene. The important regulative elements region in the promoter of hfgl2 gene was in response to HBc and HBx. which contributes to further pursuit of cis-acting elements and transcriptional factors involved in the transcription of hfgl2 gene.
