目的探讨中药癌复康对重症联合免疫缺陷(SCID)鼠原位移植人乳腺癌自发性肺转移及免疫重建的影响。方法将40只SCID小鼠随机分为4组,以高转移人乳腺癌细胞株(MDA-MB-435s)接种于鼠腹部左侧第二乳头下的乳房脂肪垫上建立小鼠人乳腺癌自发...目的探讨中药癌复康对重症联合免疫缺陷(SCID)鼠原位移植人乳腺癌自发性肺转移及免疫重建的影响。方法将40只SCID小鼠随机分为4组,以高转移人乳腺癌细胞株(MDA-MB-435s)接种于鼠腹部左侧第二乳头下的乳房脂肪垫上建立小鼠人乳腺癌自发性肺转移模型,接种量为0.2 m L/只。接种后,模型对照组给予生理盐水10 m L/(kg·d)灌胃,1次/d;癌复康组给予癌复康3.6g/(kg·d)以生理盐水10 m L稀释灌胃,1次/d;免疫重建组腹腔注射人体外周血淋巴细胞4×107个/只(0.5 m L),并给予生理盐水10 m L/(kg·d)灌胃,1次/d;癌复康+免疫重建组腹腔注射人体外周血淋巴细胞4×107个/只,并予以癌复康3.6 g/(kg·d)以生理盐水10 m L稀释灌胃,1次/d。观察各组肿瘤体积、生长速度、肿瘤转移情况。结果癌复康+免疫重建组成瘤潜伏期明显长于其余3组(P均<0.05),癌复康组和免疫重建组成瘤潜伏期明显长于模型对照组(P<0.05);模型对照组肿瘤生长速度较其余3组快,肿瘤体积大于其余3组(P均<0.05),癌复康组和免疫重建组肿瘤生长速度相近(P>0.05),癌复康+免疫重建组肿瘤生长速度最慢(P均<0.05);癌复康组、免疫重建组、癌复康+免疫重建组乳腺癌肺转移抑制率分别为64.7%,38.4%和84.8%,3组间比较差异均有统计学意义(P均<0.05)。结论中药癌复康能延长人乳腺癌细胞株MDA-MB-435s接种SCID鼠成瘤潜伏时间,促进SCID鼠的免疫重建,具有一定的抑制肿瘤生长和抗肿瘤转移的作用。展开更多
In order to quantitate the bovine immunodeficiency virus (BIV) infection in vitro, a BIV indicator cell line (BIVL) was established by transfecting baby hamster kidney cells with reporter plasmids containing the firef...In order to quantitate the bovine immunodeficiency virus (BIV) infection in vitro, a BIV indicator cell line (BIVL) was established by transfecting baby hamster kidney cells with reporter plasmids containing the firefly luciferase gene driven by a BIV long terminal repeat promoter. The BIV activates promoter activity of the LTR to express luciferase upon infection. BIV infection could therefore by quantified by detection of luciferase activity. Compared to standard assays used to detect BIV infection, the BIVL-based assay is 10 times more sensitive than the the CPE-based assay, and has similar sensitivity with the viral capsid protein Western blot assay. BIV indicator cell line could detect BIV infection specifically. Luciferase activity of BIV infected BIVL cells showed a time dependent manner, and 60 h post infection is the optimal time to detect BIV infection. Luciferase activity of BIVL cells correlates with the BIV capsid protein expression. Moreover, a linear relationship was found between MOI and the activated intensity of luciferase expression. In brief, the BIV indicator cell line is an easy, robust and quantitive method for monitoring BIV infection.展开更多
文摘目的探讨中药癌复康对重症联合免疫缺陷(SCID)鼠原位移植人乳腺癌自发性肺转移及免疫重建的影响。方法将40只SCID小鼠随机分为4组,以高转移人乳腺癌细胞株(MDA-MB-435s)接种于鼠腹部左侧第二乳头下的乳房脂肪垫上建立小鼠人乳腺癌自发性肺转移模型,接种量为0.2 m L/只。接种后,模型对照组给予生理盐水10 m L/(kg·d)灌胃,1次/d;癌复康组给予癌复康3.6g/(kg·d)以生理盐水10 m L稀释灌胃,1次/d;免疫重建组腹腔注射人体外周血淋巴细胞4×107个/只(0.5 m L),并给予生理盐水10 m L/(kg·d)灌胃,1次/d;癌复康+免疫重建组腹腔注射人体外周血淋巴细胞4×107个/只,并予以癌复康3.6 g/(kg·d)以生理盐水10 m L稀释灌胃,1次/d。观察各组肿瘤体积、生长速度、肿瘤转移情况。结果癌复康+免疫重建组成瘤潜伏期明显长于其余3组(P均<0.05),癌复康组和免疫重建组成瘤潜伏期明显长于模型对照组(P<0.05);模型对照组肿瘤生长速度较其余3组快,肿瘤体积大于其余3组(P均<0.05),癌复康组和免疫重建组肿瘤生长速度相近(P>0.05),癌复康+免疫重建组肿瘤生长速度最慢(P均<0.05);癌复康组、免疫重建组、癌复康+免疫重建组乳腺癌肺转移抑制率分别为64.7%,38.4%和84.8%,3组间比较差异均有统计学意义(P均<0.05)。结论中药癌复康能延长人乳腺癌细胞株MDA-MB-435s接种SCID鼠成瘤潜伏时间,促进SCID鼠的免疫重建,具有一定的抑制肿瘤生长和抗肿瘤转移的作用。
基金The General Foundation of Tianjin Science Committee for Applied Basic Research (08JCZDJC21000)Chinese Ministry of Education (30770081)
文摘In order to quantitate the bovine immunodeficiency virus (BIV) infection in vitro, a BIV indicator cell line (BIVL) was established by transfecting baby hamster kidney cells with reporter plasmids containing the firefly luciferase gene driven by a BIV long terminal repeat promoter. The BIV activates promoter activity of the LTR to express luciferase upon infection. BIV infection could therefore by quantified by detection of luciferase activity. Compared to standard assays used to detect BIV infection, the BIVL-based assay is 10 times more sensitive than the the CPE-based assay, and has similar sensitivity with the viral capsid protein Western blot assay. BIV indicator cell line could detect BIV infection specifically. Luciferase activity of BIV infected BIVL cells showed a time dependent manner, and 60 h post infection is the optimal time to detect BIV infection. Luciferase activity of BIVL cells correlates with the BIV capsid protein expression. Moreover, a linear relationship was found between MOI and the activated intensity of luciferase expression. In brief, the BIV indicator cell line is an easy, robust and quantitive method for monitoring BIV infection.
