Purpose: To investigate the effects on telomerase activity, human telomerase reversetranscriptase (hTERT) gene and TEP1 mRNA of retinal pigment epithelial (RPE) cells weretreated by TGF-β1 of different concentration....Purpose: To investigate the effects on telomerase activity, human telomerase reversetranscriptase (hTERT) gene and TEP1 mRNA of retinal pigment epithelial (RPE) cells weretreated by TGF-β1 of different concentration.Methods: The cultured human RPE cells were treated with TGF-β1 at differentconcentration(0 ng/ml, 0. 01 ng/ml, 0.1 ng/ml, 1 ng/ml, 10 ng/ml) for 24 h, thentelomerase activity was detected by telomerase repeat amplification protocol(TRAP). Theexpression of hTERT mRNA and TEP-1mRNA were detected by reverse transcriptionpolymerase chain reaction(RT-PCR).Results: TRAP and RT-PCR showed when the concentration of TGF-β1 was graduallyincreased, telomerase activity and the expression of hTERTmRNA were gradually reduced,TEP1mRNA showed no apparent differential expression.Conclusion: TGF-β1 can down-regulate telomerase activity and the expression of hTERTmRNA, but no effection on TEP-1mRNA, hTERTmRNA expression was in accordance withtelomerase activity in hRPE cells, hTERT gene plays a crucial role in the expression oftelomerase activity, while TEP1 plays a much smaller role.展开更多
Receiver Autonomous Integrity Monitoring （RAIM） is a software algorithm available in some GPS receivers which gives an indication if the position solution given by the GPS receiver is suitable to use. The detail alg...Receiver Autonomous Integrity Monitoring （RAIM） is a software algorithm available in some GPS receivers which gives an indication if the position solution given by the GPS receiver is suitable to use. The detail algorithm of the parity space method of RAIM technique is presented. Using FDI and FDE methods, the simulations of RAIM performance have been done in three different phases independently with respect to the bias of the fault satellite. Case study of simulation results is discussed and each performance of RAIM is analyzed. According to the analysis of simulation results, the parity space method of RAIM can meet the integrity requirements for nonprecision, terminal and enroute flight phase. It also indicates that the results of performance of FDE are better than that of FDI.展开更多
文摘Purpose: To investigate the effects on telomerase activity, human telomerase reversetranscriptase (hTERT) gene and TEP1 mRNA of retinal pigment epithelial (RPE) cells weretreated by TGF-β1 of different concentration.Methods: The cultured human RPE cells were treated with TGF-β1 at differentconcentration(0 ng/ml, 0. 01 ng/ml, 0.1 ng/ml, 1 ng/ml, 10 ng/ml) for 24 h, thentelomerase activity was detected by telomerase repeat amplification protocol(TRAP). Theexpression of hTERT mRNA and TEP-1mRNA were detected by reverse transcriptionpolymerase chain reaction(RT-PCR).Results: TRAP and RT-PCR showed when the concentration of TGF-β1 was graduallyincreased, telomerase activity and the expression of hTERTmRNA were gradually reduced,TEP1mRNA showed no apparent differential expression.Conclusion: TGF-β1 can down-regulate telomerase activity and the expression of hTERTmRNA, but no effection on TEP-1mRNA, hTERTmRNA expression was in accordance withtelomerase activity in hRPE cells, hTERT gene plays a crucial role in the expression oftelomerase activity, while TEP1 plays a much smaller role.
文摘Receiver Autonomous Integrity Monitoring （RAIM） is a software algorithm available in some GPS receivers which gives an indication if the position solution given by the GPS receiver is suitable to use. The detail algorithm of the parity space method of RAIM technique is presented. Using FDI and FDE methods, the simulations of RAIM performance have been done in three different phases independently with respect to the bias of the fault satellite. Case study of simulation results is discussed and each performance of RAIM is analyzed. According to the analysis of simulation results, the parity space method of RAIM can meet the integrity requirements for nonprecision, terminal and enroute flight phase. It also indicates that the results of performance of FDE are better than that of FDI.
