In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are i...In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are involved in various biochemical processes,but the role of microRNAs(miRNAs)in this process is still unclear.From the Gene Expression Omnibus(GEO)database,we downloaded two microarray datasets for GSE78731(messenger RNA(mRNA)profile)and GSE97532(miRNA profile).The differentially expressed genes screened were compared between the hUMSC group and the middle cerebral artery occlusion group.Gene ontology enrichment and pathway enrichment analyses were subsequently conducted using the online Database for Annotation,Visualization,and Integrated Discovery.Identified genes were applied to perform weighted gene co-suppression analyses,to establish a weighted co-expression network model.Furthermore,the protein-protein interaction network for differentially expressed genes from turquoise modules was built using Cytoscape(version 3.40)and the most highly correlated subnetwork was extracted from the protein-protein interaction network using the MCODE plugin.The predicted target genes for differentially expressed miRNAs were also identified using the online database starBase v3.0.A total of 3698 differentially expressed genes were identified.Gene ontology analysis demonstrated that differentially expressed genes that are related to hUMSC treatment of ischemic cerebral infarction are involved in endocytosis and inflammatory responses.We identified 12 differentially expressed miRNAs in middle cerebral artery occlusion rats after hUMSC treatment,and these differentially expressed miRNAs were mainly involved in signaling in inflammatory pathways,such as in the regulation of neutrophil migration.In conclusion,we have identified a number of differentially expressed genes and differentially expressed mRNAs,miRNA-mRNAs,and signaling pathways involved in the hUMSC treatment of ischemic cerebral infarction.Bioinformatics and interaction analyses can provide novel clues for further research into hUMSC treatment of ischemic cerebral infarction.展开更多
目的:探讨脐带间充质干细胞(HUMSCs)移植对老年痴呆小鼠SAMP8学习记忆能力的影响。方法:培养人脐带间充质干细胞,通过尾静脉以高中低3个剂量(2.5×105/0.2 m L/20 g、5×105/0.2 m L/20 g、1×106/0.2 m L/20 g)分别向7月龄...目的:探讨脐带间充质干细胞(HUMSCs)移植对老年痴呆小鼠SAMP8学习记忆能力的影响。方法:培养人脐带间充质干细胞,通过尾静脉以高中低3个剂量(2.5×105/0.2 m L/20 g、5×105/0.2 m L/20 g、1×106/0.2 m L/20 g)分别向7月龄SAMP8老年痴呆小鼠移植HUMSCs,并设立移植生理盐水组和R1小鼠正常对照组。HUMSCs移植45 d后,MORRIS水迷宫和跳台实验检测其学习记忆能力。结果:与R1正常对照小鼠比较,SAMP8老年痴呆小鼠生理盐水组学习记忆能力明显降低;和移植生理盐水组比较,细胞移植低剂量组水迷宫逃避潜伏期显著缩短(P<0.05),穿越平台次数显著增加(P<0.05);跳台潜伏期显著延长(P<0.05),错误次数显著减少(P<0.05),结论:低剂量的脐带间充质干细胞移植能增强老年痴呆小鼠SAMP8的学习记忆能力。展开更多
Human umbilical cord mesenchymal stem cells(HuMSCs)have the multi-difFerentiation potential to differentiate into various types of cells without immune rejection.They are considered to be an ideal source of neural ste...Human umbilical cord mesenchymal stem cells(HuMSCs)have the multi-difFerentiation potential to differentiate into various types of cells without immune rejection.They are considered to be an ideal source of neural stem cells and also an ideal cell carrier for gene therapy.Because of the invasive growth of brain gliomas,most of them have no obvious boundaries with normal brain tissues.It is difficult to completely remove them by surgery and the remaining cells become the main source of tumor recurrence.In recent years,gene therapy has become a new method for the treatment of gliomas.The vector carrying the target gene is introduced into HuMSCs in a certain way to correct gene defects or play other roles.The differentiation potential of HuMSCs makes it an ideal source of nerve cells to play a greater role in gene therapy of glioma.Therefore,this article reviews the current status and prospects of HuMSCs as cell carriers in the treatment of glioma.展开更多
Islet-1(Isl1),a LIM homeodomain protein,is expressed in the embryonic pancreatic epithelium.As a key transcription factor,Isl1 can not only regulate insulin gene expression in normal glucose condition but also maintai...Islet-1(Isl1),a LIM homeodomain protein,is expressed in the embryonic pancreatic epithelium.As a key transcription factor,Isl1 can not only regulate insulin gene expression in normal glucose condition but also maintainβ-cell function and impact pancreaticβ-cell target genes.Some experiments have suggested that Micro RNA(miRNA)can play a critical role during the induction of insulinproducing cells(IPCs).However,it is unclear whether miRNA may regulate Isl1 expression during differentiation of human umbilical cord mesenchymal stem cells(HUMSCs)into IPCs.In this investigation,we induced HUMSCs into IPCs with a modified two-step protocol,activin A,retinoic acid(step1)and conophylline,nicotinamide(step2).To find the miRNA regulating Isl1 expression,we respectively used Target Scan,miRDB and RNAhybrid to predict and got the result,miR-128 and miR-216a.The miRNAs can inhibit Isl1 expression by dual luciferase assay.The results of real-time Polymerase Chain Reaction(PCR)showed that Isl1 expression level was almost reciprocal to that of miR-128 and miR-216a during differentiation of HUMSCs into IPCs.Furthermore,over-expression of miR-128 or miR-216a downregulated expression levels of Isl1 and Maf A.Therefore,miR-128 or miR-216a may regulate expression of islet-specific transcription factors to control differentiation of HUMSCs into IPCs.展开更多
基金supported by the National Key Research&Development Program of China,No.2016YFC1301600Program for Jilin University Science and Technology Innovation Team,No.2017TD-12(both to YY)
文摘In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are involved in various biochemical processes,but the role of microRNAs(miRNAs)in this process is still unclear.From the Gene Expression Omnibus(GEO)database,we downloaded two microarray datasets for GSE78731(messenger RNA(mRNA)profile)and GSE97532(miRNA profile).The differentially expressed genes screened were compared between the hUMSC group and the middle cerebral artery occlusion group.Gene ontology enrichment and pathway enrichment analyses were subsequently conducted using the online Database for Annotation,Visualization,and Integrated Discovery.Identified genes were applied to perform weighted gene co-suppression analyses,to establish a weighted co-expression network model.Furthermore,the protein-protein interaction network for differentially expressed genes from turquoise modules was built using Cytoscape(version 3.40)and the most highly correlated subnetwork was extracted from the protein-protein interaction network using the MCODE plugin.The predicted target genes for differentially expressed miRNAs were also identified using the online database starBase v3.0.A total of 3698 differentially expressed genes were identified.Gene ontology analysis demonstrated that differentially expressed genes that are related to hUMSC treatment of ischemic cerebral infarction are involved in endocytosis and inflammatory responses.We identified 12 differentially expressed miRNAs in middle cerebral artery occlusion rats after hUMSC treatment,and these differentially expressed miRNAs were mainly involved in signaling in inflammatory pathways,such as in the regulation of neutrophil migration.In conclusion,we have identified a number of differentially expressed genes and differentially expressed mRNAs,miRNA-mRNAs,and signaling pathways involved in the hUMSC treatment of ischemic cerebral infarction.Bioinformatics and interaction analyses can provide novel clues for further research into hUMSC treatment of ischemic cerebral infarction.
文摘目的:探讨脐带间充质干细胞(HUMSCs)移植对老年痴呆小鼠SAMP8学习记忆能力的影响。方法:培养人脐带间充质干细胞,通过尾静脉以高中低3个剂量(2.5×105/0.2 m L/20 g、5×105/0.2 m L/20 g、1×106/0.2 m L/20 g)分别向7月龄SAMP8老年痴呆小鼠移植HUMSCs,并设立移植生理盐水组和R1小鼠正常对照组。HUMSCs移植45 d后,MORRIS水迷宫和跳台实验检测其学习记忆能力。结果:与R1正常对照小鼠比较,SAMP8老年痴呆小鼠生理盐水组学习记忆能力明显降低;和移植生理盐水组比较,细胞移植低剂量组水迷宫逃避潜伏期显著缩短(P<0.05),穿越平台次数显著增加(P<0.05);跳台潜伏期显著延长(P<0.05),错误次数显著减少(P<0.05),结论:低剂量的脐带间充质干细胞移植能增强老年痴呆小鼠SAMP8的学习记忆能力。
文摘Human umbilical cord mesenchymal stem cells(HuMSCs)have the multi-difFerentiation potential to differentiate into various types of cells without immune rejection.They are considered to be an ideal source of neural stem cells and also an ideal cell carrier for gene therapy.Because of the invasive growth of brain gliomas,most of them have no obvious boundaries with normal brain tissues.It is difficult to completely remove them by surgery and the remaining cells become the main source of tumor recurrence.In recent years,gene therapy has become a new method for the treatment of gliomas.The vector carrying the target gene is introduced into HuMSCs in a certain way to correct gene defects or play other roles.The differentiation potential of HuMSCs makes it an ideal source of nerve cells to play a greater role in gene therapy of glioma.Therefore,this article reviews the current status and prospects of HuMSCs as cell carriers in the treatment of glioma.
基金Supported by Liaoning Province Education Administration Funded Program of China(LJKZ1374)。
文摘Islet-1(Isl1),a LIM homeodomain protein,is expressed in the embryonic pancreatic epithelium.As a key transcription factor,Isl1 can not only regulate insulin gene expression in normal glucose condition but also maintainβ-cell function and impact pancreaticβ-cell target genes.Some experiments have suggested that Micro RNA(miRNA)can play a critical role during the induction of insulinproducing cells(IPCs).However,it is unclear whether miRNA may regulate Isl1 expression during differentiation of human umbilical cord mesenchymal stem cells(HUMSCs)into IPCs.In this investigation,we induced HUMSCs into IPCs with a modified two-step protocol,activin A,retinoic acid(step1)and conophylline,nicotinamide(step2).To find the miRNA regulating Isl1 expression,we respectively used Target Scan,miRDB and RNAhybrid to predict and got the result,miR-128 and miR-216a.The miRNAs can inhibit Isl1 expression by dual luciferase assay.The results of real-time Polymerase Chain Reaction(PCR)showed that Isl1 expression level was almost reciprocal to that of miR-128 and miR-216a during differentiation of HUMSCs into IPCs.Furthermore,over-expression of miR-128 or miR-216a downregulated expression levels of Isl1 and Maf A.Therefore,miR-128 or miR-216a may regulate expression of islet-specific transcription factors to control differentiation of HUMSCs into IPCs.
