Transgenic corn was modified to combat lepidopteran pests, herbicide and other deteriorate environmental factors. However, fewer studies concerned the effects of transgenic corn cultivation on the soil Collembola in b...Transgenic corn was modified to combat lepidopteran pests, herbicide and other deteriorate environmental factors. However, fewer studies concerned the effects of transgenic corn cultivation on the soil Collembola in black soil region in China. In Jilin black soil region, a six treatments study including three corn varieties (transgenic corn, control and local variety) and two sampling locations (in rizhosphere and in between plants) were deployed to study the effect of transgenic corn cultivation and sampling location on densities, species richness and community structure of Collembola. We found that root biomass was marginally decreased in transgenic corn and control variety when compared to local variety. Collembola significantly affected by sampling location treatments. Densities and species richness of collembolans significantly decreased in "between plants" than in "rizhosphere". Densities of Collembola were impacted by corn varieties. In the whole growing season, densities of Collembola significantly decreased in control than in local variety. Collembolans in transgenic variety only slightly decreased in "jointing stage" than local variety. Collembolans adversely affected in "between plants" when compared to "in rhizosphere" in "seeding stage" but not other stages. These variations of collembolans were in lines with the trend of corn root biomass. It is indicated that Collembola variations could be caused by food resources (root biomass) but not corn varieties (transgenic, control and local).展开更多
Habit is on the wane in the discourse of education theory, although it stillmaintains great vitality in everyday discourse. It is fair to say that habitas a concept has been distorted by behaviorism. To remove the imp...Habit is on the wane in the discourse of education theory, although it stillmaintains great vitality in everyday discourse. It is fair to say that habitas a concept has been distorted by behaviorism. To remove the impactof behaviorism, we need to review and reconstruct the concept of habit.Habits shape “self” and also nourish virtue. Habits concern mind andbody, which means they feature “corporeal nature” and “mental nature”.When in action, habits seem to exhibit a double law, but in effect follow asingle law. Education plays an important role in habit formation. Educationis the environmental factor of habit formation and it cultivates habitsthrough earnest practice and careful deliberation. In addition, education isalso an important approach to habit change.展开更多
Peanut (Arachis hypogaea L.) production is valued at $1.28 billion annually in the USA. Plant growth habit can be used to determine plant population density and cultivation practices a given farmer uses. Erect plants ...Peanut (Arachis hypogaea L.) production is valued at $1.28 billion annually in the USA. Plant growth habit can be used to determine plant population density and cultivation practices a given farmer uses. Erect plants are generally more compact and can be more densely planted unlike plants with more prostrate growth. The objectives of this study were to analyze publicly available datasets to identify single-nucleotide polymorphism (SNP) markers associated with plant growth habit in peanuts and to conduct genomic selection. A genome-wide association study (GWAS) was used to identify SNPs for growth habit type among 775 USDA peanut accessions. A total of 13,306 SNPs were used to conduct GWAS using five statistical models. The models used were single-marker regression, generalized linear model (PCA), generalized linear model (Q), mixed linear model (PCA), and mixed linear model (Q) and a total of 181, 1, 108, 1, and 10 SNPs were found associated with growth habit respectively. Based on this dataset, results showed that genomic selection can achieve up to 61% accuracy, depending on the training population size being used for the prediction. SNP AX-176821681 was found in all models. Gene ontology for this location shows an annotated gene, Araip.0F3YM, found 2485 bp upstream of this SNP and encodes for a peptidyl-prolyl cis-trans isomerase. To the best of our knowledge, this is the first report identifying molecular markers linked to plant growth habit type in peanuts. This finding suggests that a molecular marker can be developed to identify specific plant growth habits in peanuts, enabling early generation selection by peanut breeders.展开更多
Under the background of the all-round deepening of quality education,the cultivation of comprehensive quality has become the main theme of contemporary education reform.Good behavior and habits are of great significan...Under the background of the all-round deepening of quality education,the cultivation of comprehensive quality has become the main theme of contemporary education reform.Good behavior and habits are of great significance to children’s future learning,growth,and development.Through literature review and other methods,this paper analyzes the current situation of children’s family education and the influence of family education on the cultivation of children’s behavioral habits and provides some strategies for cultivating children’s good behavioral habits in family education.展开更多
Objective:Cardiovascular disease(CVD)remains a significant public health challenge in China.Accurateperception of individual CVD risk is crucial for timely intervention and preventive strategies.This studyaimed to det...Objective:Cardiovascular disease(CVD)remains a significant public health challenge in China.Accurateperception of individual CVD risk is crucial for timely intervention and preventive strategies.This studyaimed to determine the alignment between CVD risk perception levels and objectively calculated CVDrisk levels,then investigate the disparity in physical activity and healthy diet habits among distinct CVDrisk perception categories.Methods:From March to August 2022,a cross-sectional survey was conducted in Zhejiang Province usingconvenience sampling.Participants aged between 20 and 80 years,without prior diagnosis of CVD wereincluded.CVD risk perception was evaluated with the Chinese version of the Attitude and Beliefs aboutCardiovascular Disease Risk Perception Questionnaire,while objective CVD risk was assessed through thePrediction for Atherosclerotic Cardiovascular Disease Risk(China-PAR)model.Participants’demographicinformation,self-reported physical activity,and healthy diet score were also collected.Results:A total of 739 participants were included in the final analysis.Less than a third of participants(29.2%)accurately perceived their CVD risk,while 64.5%over-perceived it and 6.2%under-perceived it.Notably,half of the individuals(50.0%)with high CVD risk under-perceived their actual risk.Compared tothe accurate perception group,individuals aged 60e80 years old(OR=6.569),currently drinking(OR=3.059),and with hypertension(OR=2.352)were more likely to under-perceive their CVD risk.Meanwhile,participants aged 40-<60 years old(OR=2.462)and employed(OR=2.352)were morelikely to over-perceive their risk.The proportion of individuals engaging in physical activity was lowest inthe under-perception group,although the difference among the three groups was not statistically significant(X^(2)=2.556,P=0.278).In addition,the proportion of individuals practicing healthy diet habitswas also lowest in the under-perception group,and a significant statistical difference was observedamong the three groups(X^(2)=10.310,P=0.006).Conclusion:Only a small proportion of participants accurately perceived their CVD risk,especially amongthose with high actual CVD risk.Individuals in the under-perceived CVD risk group exhibited the lowestrates of physical activity engagement and healthy diet adherence.Healthcare professionals should prioritize implementing personalized CVD risk communication strategies tailored to specific subgroups toenhance the accuracy of risk perception.展开更多
Plasma nitrogen fixation(PNF)has been emerging as a promising technology for greenhouse gasfree and renewable energy-based agriculture.Yet,most PNF studies seldom address practical application-specific issues.In this ...Plasma nitrogen fixation(PNF)has been emerging as a promising technology for greenhouse gasfree and renewable energy-based agriculture.Yet,most PNF studies seldom address practical application-specific issues.In this work,we present the development of a compact and automatic PNF system for on-site agricultural applications.The system utilized a gliding-arc discharge as the plasma source and employed a dual-loop design to generate NO_(x)from air and water under atmospheric conditions.Experimental results showed that the system with a dualloop design performs well in terms of energy costs and production rates.Optimal operational parameters for the system were determined through experimentation,resulting in an energy cost of 13.9 MJ mol^(-1)and an energy efficiency of 16 g kWh^(-1)for NO_(3)^(-)production,respectively.Moreover,the concentration of exhausted NO_(x)was below the emission standards.Soilless lettuce cultivation experiments demonstrated that NO_(x)^(-)produced by the PNF system could serve as liquid nitrate nitrogen fertilizer.Overall,our work demonstrates the potential of the developed PNF system for on-site application in the production of green-leaf vegetables.展开更多
The possibility of the life origin in the stellar systems, located at a distance of ~200 pc from the solar system, was investigated. The stars, in the spectrums of which C (carbon), O (oxygen), N (nitrogen), and P (ph...The possibility of the life origin in the stellar systems, located at a distance of ~200 pc from the solar system, was investigated. The stars, in the spectrums of which C (carbon), O (oxygen), N (nitrogen), and P (phosphorus) are found, are called DNA-stars. Based on stellar abundances a new method for searching for habitable exoplanets has been developed and a list of 48 DNA-stars in the solar neighborhood, on which life is possible, has been defined. The quota of DNA-stars is equal 1.3% of the total amount of Hypatia Stellar Catalog. Only three DNA-stars out of selected 48 stars belong to the spectral class as our Sun (G2V). The closest to the solar system is the DNA-star with the number HIP 15510, which belongs to the G8V class and is 6 pc away from the solar system. Nine DNA-stars, which have the highest chemical similarity with solar spectrum, were identified. It is identified that one of these nine stars, HIP 24681, has six planets.展开更多
Appropriate physical exercise has a positive impact on adolescents’physical and mental health,but there is a serious lack of physical exercise among Chinese adolescents.How to shape their exercise behavior(EB)has bec...Appropriate physical exercise has a positive impact on adolescents’physical and mental health,but there is a serious lack of physical exercise among Chinese adolescents.How to shape their exercise behavior(EB)has become an important task in promoting their development.A questionnaire survey was conducted using stratified cluster random sampling on three middle schools by class in Zhejiang Province,China to investigate the impact of exercise atmosphere(EA)on adolescents’exercise behavior and the mediating role of exercise identity(EI)and exercise habit(EH).806 adolescents were investigated by the Exercise Atmosphere Scale(EAS),Exercise-Identity Scale(EIS),Self-Report Habit Index(SRHI),and Physical Activity Rating Scale(PARS-3).The results show that:There is a significant positive correlation between each two of exercise atmosphere,exercise identity,exercise habit,and exercise behavior(p<0.05).Exercise atmosphere could not only directly affect adolescents’physical exercise behavior but can also indirectly affect their physical exercise behavior through the mediating effect of exercise identity and exercise habit,involving three mediating pathways,namely,the mediating path through exercise identity,the mediating pathway through exercise habit and the chain mediating pathway through exercise identity and exercise habit.The direct effect of exercise atmosphere on exercise behavior was 0.459(p<0.01),accounting for 62.62% of the total effect of 0.733,and its indirect effect was 0.274,accounting for 37.28% of the total effect.To a certain extent,the mediating effect model reveals the mechanism of exercise atmosphere affecting exercise behavior and has a certain reference value for promoting adolescents’exercise behavior.We should start by creating an exercise atmosphere,cultivating exercise identity,and enhancing exercise habits to help teenagers form active physical exercise behaviors.展开更多
This research advocates for the construction of Climate Change Haven Communities across the Appalachian Region. The proposed development plan can be extended to the northern tier states across the US and also to the n...This research advocates for the construction of Climate Change Haven Communities across the Appalachian Region. The proposed development plan can be extended to the northern tier states across the US and also to the northern and mountainous regions of Europe and Asia. We present an analogy to the earlier climate change period of the Last Glacial Maximum/“Ice Age” in which these same northern regions of the planet were covered in ice sheets making them uninhabitable for most humans and many plant and animal species. In some significant ways, the Ice Age scenario can be a reverse-model for our current climate crisis. We also advocate strongly for the prevention of upscale real estate development projects in these same regions of the globe, as these will foreclose the possibility of safely sheltering the millions of persons who will be displaced by climate change over the next 5 to 10 years.展开更多
A high-efficiency mode of high-low seedbed cultivation(HLSC)has been listed as the main agricultural technology to increase land utilization ratio and grain yield in Shandong Province,China.However,limited information...A high-efficiency mode of high-low seedbed cultivation(HLSC)has been listed as the main agricultural technology to increase land utilization ratio and grain yield in Shandong Province,China.However,limited information is available on the optimized water and nitrogen management for yield formation,especially the grain-filling process,under HLSC mode.A three-year field experiment with four nitrogen rates and three irrigation rates of HLSC was conducted to reveal the response of grain-filling parameters,grain weight percentage of spike weight(GPS),spike moisture content(SMC),and winter wheat yield to water and nitrogen rates.The four nitrogen rates were N1(360 kg ha^(-1) pure N),N2(300 kg ha^(-1) pure N),N3(240 kg ha^(-1) pure N),and N4(180 kg ha^(-1) pure N),respectively,and the three irrigation quotas were W1(120 mm),W2(90 mm),and W3(60 mm),respectively.Results showed that the determinate growth function generally performed well in simulating the temporal dynamics of grain weight(0.989<R^(2)<0.999,where R2 is the determination coefficient).The occurrence time of maximum filling rate(T_(max))and active grain-filling period(AGP)increased with the increase in the water or nitrogen rate,whereas the average grain-filling rate(G_(mean))had a decreasing trend.The final 1,000-grain weight(FTGW)increased and then decreased with the increase in the nitrogen rates and increased with the increase in the irrigation rates.The GPS and SMC had a highly significant quadratic polynomial relationship with grain weight and days after anthesis.Nitrogen,irrigation,and year significantly affected the T_(max),AGP,G_(mean),and FTGW.Particularly,the AGP and FTGW were insignificantly different between high seedbed(HLSC-H)and low seedbed(HLSC-L)across the water and nitrogen levels.Moreover,the moderate water and nitrogen supply was more beneficial for grain yield,as well as for spike number and grain number per hectare.The principal component analysis indicated that combining 240-300 kg N ha^(-1) and 90^(-1)20 mm irrigation quota could improve grain-filling efficiency and yield for the HLSC-cultivated winter wheat.展开更多
Strawberry (Fragaria spp.) is one of the most important fruits classified as exotic fruits imported into Cameroon. To have an inventory of its cultivation in Cameroon, a survey study was carried out among eight farms ...Strawberry (Fragaria spp.) is one of the most important fruits classified as exotic fruits imported into Cameroon. To have an inventory of its cultivation in Cameroon, a survey study was carried out among eight farms of Fragaria spp. from January 2021 to February 2022. The plant was introduced in Cameroon in 2018. There are 13 varieties of Fragaria spp. currently cultivated. Among these 13 varieties, eleven are hybrids of Fragaria x ananassa (“Amiga”, “Amine”, “Camarosa”, “Chandler”, “Charlotte”, “Elsanta”, “Gariguette”, “Madame Moutot”, “Ostara”, “Ruby gem” and “San Andreas”), and two of the hybrids of Fragaria vesca (“Maestro” and “Mara des bois”). The cropping system, irrigation system, and type of fertilizers applied differ from one strawberry farm to another. Biofertilizers (such as mycorrhizal), inorganic and organic fertilizers are actually used to improve production. The potential annual production of strawberries from January 2021 to February 2022, estimated based on the survey data, was 21.216 tons for all growers. Among these eight production farms, the Lolodorf BIO Farm presents 6000 kg (six tons) of strawberries and 100,000 stolons (seedlings) produced, from seven varieties of Fragaria spp. cultivated, with 6 varieties which are hybrids variety Fragaria x ananassa (“Amiga”, “Amine”, “Chandler”, “Gariguette”, “Madame Moutot”, and “Ruby gem”), and one which is a hybrid of Fragaria vesca (“Mara des bois”). Certain diseases were also observed and recorded depending on the growing areas.展开更多
Objective: The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students. To explore the method and effect of training system of scientific rese...Objective: The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students. To explore the method and effect of training system of scientific research innovation ability of nursing undergraduates based on “3332”. Methods: Three course learning modules are constructed: stage-based course learning module, systematic project practice training module and comprehensive practice training module. A practical training platform for scientific research innovation projects is built, and undergraduate scientific research innovation ability training is carried out from both in-class and out-of-class lines. Results: Since 2017, the students have obtained 7 national innovation and entrepreneurship training programs, 52 university-level undergraduate scientific research projects, published more than 10 academic papers, and obtained 2 patent authorization. Conclusions: The training system of scientific research innovation ability of nursing undergraduates based on “3332” is conducive to the development of scientific research innovation ability of nursing students, and to cultivate nursing talents who can adapt to the development of the new era and have better post competence.展开更多
Integrative cultivation practices(ICPs)are essential for enhancing cereal yield and resource use efficiency.However,the effects of ICP on the rhizosphere environment and roots of paddy rice are still poorly understood...Integrative cultivation practices(ICPs)are essential for enhancing cereal yield and resource use efficiency.However,the effects of ICP on the rhizosphere environment and roots of paddy rice are still poorly understood.In this study,four rice varieties were produced in the field.Each variety was treated with six different cultivation techniques,including zero nitrogen application(0 N),local farmers’practice(LFP),nitrogen reduction(NR),and three progressive ICP techniques comprised of enhanced fertilizer N practice and increased plant density(ICP1),a treatment similar to ICP1 but with alternate wetting and moderate drying instead of continuous flooding(ICP2),and the same practices as ICP2 with the application of organic fertilizer(ICP3).The ICPs had greater grain production and nitrogen use efficiency than the other three methods.Root length,dry weight,root diameter,activity of root oxidation,root bleeding rate,zeatin and zeatin riboside compositions,and total organic acids in root exudates were elevated with the introduction of the successive cultivation practices.ICPs enhanced nitrate nitrogen,the activities of urease and invertase,and the diversity of microbes(bacteria)in rhizosphere and non-rhizosphere soil,while reducing the ammonium nitrogen content.The nutrient contents(ammonium nitrogen,total nitrogen,total potassium,total phosphorus,nitrate,and available phosphorus)and urease activity in rhizosphere soil were reduced in all treatments in comparison with the non-rhizosphere soil,but the invertase activity and bacterial diversity were greater.The main root morphology and physiology,and the ammonium nitrogen contents in rhizosphere soil at the primary stages were closely correlated with grain yield and internal nitrogen use efficiency.These findings suggest that the coordinated enhancement of the root system and the environment of the rhizosphere under integrative cultivation approaches may lead to higher rice production.展开更多
Polycarbonate plastics containing bisphenol A (BPA) used to manufacture drinking water bottles. Kurdistan region in northern Iraq is a developed area with increased pollution from plastic bottles. Trace amounts of BPA...Polycarbonate plastics containing bisphenol A (BPA) used to manufacture drinking water bottles. Kurdistan region in northern Iraq is a developed area with increased pollution from plastic bottles. Trace amounts of BPA have been detected in bottled water samples. The absorption of BPA was measured with HPLC using a vertical cultivation system with Bulbs of the Allium Cepa plant planted in these plastic bottles with monitored growth. Vertical cultivation was found to have a low level of BPA in the plant cells, making it a safe cultivation method under specific climate conditions. The mean concentration of BPA in vertical cultivation is 0.19 ug/ml (3.8 ng for a 20 uL injection), and the Limit of Quantification (LOQ) is 0.63 ug/ml (12.7 ng for 20 uL injection). While Scanning Electron Microscope (SEM) shows that the concentrations are relatively low in water samples stored at room temperature compared to those exposed to direct sunlight (40°C) and water bottle samples stored at (-4°C), The correlation coefficients were found to be good (0.9992). SEM is used for plastic bottle samples stored at different temperatures. The images identify compound decay and explore the morphology of BPA in manufactured plastic materials.展开更多
Tobacco is an essential cash crop in Zimbabwe and a strategic livelihood option for hundreds of thousands of rural households. However, the crop is linked to negative environmental, economic, and social impacts. The e...Tobacco is an essential cash crop in Zimbabwe and a strategic livelihood option for hundreds of thousands of rural households. However, the crop is linked to negative environmental, economic, and social impacts. The existing studies on tobacco cultivation in Zimbabwe present contradictory findings on the determinants and impacts of adoption, leaving unanswered questions about the crop’s sustainability impact in the country. This article investigates the determinants of smallholder farmers’ decisions to grow tobacco and the associated impacts of adoption. Random and purposive sampling were used to select 273 household surveys, including tobacco and non-tobacco smallholder farmers, and 56 expert interviews to answer the research questions. We employed regression models alongside expert interviews and document analysis to identify the determinants influencing the decision-making process of smallholder farmers in Zimbabwe regarding tobacco cultivation. Additionally, our investigation aimed to elucidate the perceived impacts associated with the adoption of this agricultural practice. The regression analysis indicated that the farmer’s age, education level, farming experience, family size, household income, and perceived high farm profitability are significant drivers of tobacco adoption. We also discovered divergent and convergent perceptions of the critical impacts of tobacco cultivation. The study highlights the need for proactive multi-stakeholder collaboration and sustainable financial arrangements to address the negative impacts of tobacco production. As the primary stakeholder responsible for regulating and promoting agricultural activities, the Zimbabwean government should provide meaningful financial support, increase access to credit, and ensure better market facilities for alternative crops to reduce the over-dependence on tobacco.展开更多
BACKGROUND Cognitive-behavioral therapy(CBT)and habit reversal training(HRT)have shown application potential in addressing tic symptoms and comorbid psychiatric conditions.Despite their theoretical potential,empirical...BACKGROUND Cognitive-behavioral therapy(CBT)and habit reversal training(HRT)have shown application potential in addressing tic symptoms and comorbid psychiatric conditions.Despite their theoretical potential,empirical evidence on their combined efficacy remains limited.AIM To evaluate the efficacy of CBT combined with HRT on anxiety disorders in children with Tourette’s syndrome(TS).METHODS Clinical data of children with TS admitted to our hospital from January 2022 to June 2023 were collected,and the patients were grouped into the conventional therapy(control)group and the CBT combined with HRT group.Baseline charac-teristics,anxiety scores,tic severity scores,treatment adherence,and parental satisfaction were assessed.Statistical analysis was performed using t-tests,chi-square tests,and correlation analysis.RESULTS A total of 136 patients,including 65 patients in the control group and 71 patients in the CBT combined with HRT group,were included.The CBT combined with HRT group showed remarkable improvements compared with the control group.Post-intervention assessment revealed a decrease in anxiety scores from 63.52±1.81 to 40.53±1.64(t=2.022,P=0.045),and the Yale Global Tic Severity Scale total score decreased from 22.14±5.67 to 16.28±4.91(t=2.288,P=0.024).Treatment adherence was significantly higher in the CBT combined with HRT group(85.47±7.62%)compared with the control group(82.32±6.54%;t=2.596,P=0.010).Parental satisfaction scores were also higher in the CBT combined with HRT group(8.69±1.77)compared with the control group(7.87±1.92;t=2.592,P=0.011).CONCLUSION This study demonstrates that CBT combined with HRT significantly reduces anxiety symptoms and tic severity in children with TS,with higher treatment adherence and parental satisfaction.These findings support the potential application of this comprehensive therapeutic approach for TS treatment.展开更多
The human being must find a dwelling and protect himself against the vicissitudes of nature.He has to adapt himself to the contemporary world and to his environment.Sometimes,he should accept unwittingly extreme situa...The human being must find a dwelling and protect himself against the vicissitudes of nature.He has to adapt himself to the contemporary world and to his environment.Sometimes,he should accept unwittingly extreme situations dominated by inhabitability and as such abandon his plot of land and properties and to get into the city where he has to refuge into spaces that any human being enjoying a minimum of living conditions would not accept:the slum.For the time being,he does his best to make his environment enjoyable and to transfer his existence into something of a temporary happiness by trying to make order in these inhospitable places in order to create a sort of happiness that he is the only one who can measure its real impact.Throughout this essay,we are trying to present this human habitation that we often meet in Constantine.展开更多
BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine...BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an important factor in maintaining their long-term expansion,which is essential for shortening the time of drug sensitivity analysis and is the basis of PC research.展开更多
The production environment of greenhouse cultivation is relatively closed,the multiple cropping index is high,the management of fertilizationwatering and pesticideapplication isblindtosomeextent,andthe phenomenonofcon...The production environment of greenhouse cultivation is relatively closed,the multiple cropping index is high,the management of fertilizationwatering and pesticideapplication isblindtosomeextent,andthe phenomenonofcontinuous cropping isalsocommonSoilquali-ty affects the sustainable development of greenhouse cultivation.Earthworm is a ubiquitous invertebrate organism in soil,an important part of soil system,a link between terrestrial organisms and soil organisms,an important link in the small cycle of soil material organisms,and plays an important role in maintaining the structure and function of soil ecosystem.Different ecotypes of earthworms are closely related to their habi-tats(soil layers)and food resource preferences,and then affect their ecological functions.The principle of earthworm regulating soil function is essentially the close connection and interaction between earthworm and soil microorganism.Using different ecotypes of earthworms and bio-logical agents to carry out combined remediation of greenhouse cultivation soil is a technical model to realize sustainable development of green-house cultivation.展开更多
In order to enhance the yield and quality of cashew,it is essential to implement high-yield cultivation techniques effectively throughout the production process.Additionally,pest control measures should be employed to...In order to enhance the yield and quality of cashew,it is essential to implement high-yield cultivation techniques effectively throughout the production process.Additionally,pest control measures should be employed to provide technical support for the industrialized development of cashew.展开更多
文摘Transgenic corn was modified to combat lepidopteran pests, herbicide and other deteriorate environmental factors. However, fewer studies concerned the effects of transgenic corn cultivation on the soil Collembola in black soil region in China. In Jilin black soil region, a six treatments study including three corn varieties (transgenic corn, control and local variety) and two sampling locations (in rizhosphere and in between plants) were deployed to study the effect of transgenic corn cultivation and sampling location on densities, species richness and community structure of Collembola. We found that root biomass was marginally decreased in transgenic corn and control variety when compared to local variety. Collembola significantly affected by sampling location treatments. Densities and species richness of collembolans significantly decreased in "between plants" than in "rizhosphere". Densities of Collembola were impacted by corn varieties. In the whole growing season, densities of Collembola significantly decreased in control than in local variety. Collembolans in transgenic variety only slightly decreased in "jointing stage" than local variety. Collembolans adversely affected in "between plants" when compared to "in rhizosphere" in "seeding stage" but not other stages. These variations of collembolans were in lines with the trend of corn root biomass. It is indicated that Collembola variations could be caused by food resources (root biomass) but not corn varieties (transgenic, control and local).
基金This paper is a phased research result of“studies on the theoretical deepening and practical promotion of moral education in life”(16JJD880027),a major project of the Key Research Base for Humanities and Social Sciences of the Ministry of Education.
文摘Habit is on the wane in the discourse of education theory, although it stillmaintains great vitality in everyday discourse. It is fair to say that habitas a concept has been distorted by behaviorism. To remove the impactof behaviorism, we need to review and reconstruct the concept of habit.Habits shape “self” and also nourish virtue. Habits concern mind andbody, which means they feature “corporeal nature” and “mental nature”.When in action, habits seem to exhibit a double law, but in effect follow asingle law. Education plays an important role in habit formation. Educationis the environmental factor of habit formation and it cultivates habitsthrough earnest practice and careful deliberation. In addition, education isalso an important approach to habit change.
文摘Peanut (Arachis hypogaea L.) production is valued at $1.28 billion annually in the USA. Plant growth habit can be used to determine plant population density and cultivation practices a given farmer uses. Erect plants are generally more compact and can be more densely planted unlike plants with more prostrate growth. The objectives of this study were to analyze publicly available datasets to identify single-nucleotide polymorphism (SNP) markers associated with plant growth habit in peanuts and to conduct genomic selection. A genome-wide association study (GWAS) was used to identify SNPs for growth habit type among 775 USDA peanut accessions. A total of 13,306 SNPs were used to conduct GWAS using five statistical models. The models used were single-marker regression, generalized linear model (PCA), generalized linear model (Q), mixed linear model (PCA), and mixed linear model (Q) and a total of 181, 1, 108, 1, and 10 SNPs were found associated with growth habit respectively. Based on this dataset, results showed that genomic selection can achieve up to 61% accuracy, depending on the training population size being used for the prediction. SNP AX-176821681 was found in all models. Gene ontology for this location shows an annotated gene, Araip.0F3YM, found 2485 bp upstream of this SNP and encodes for a peptidyl-prolyl cis-trans isomerase. To the best of our knowledge, this is the first report identifying molecular markers linked to plant growth habit type in peanuts. This finding suggests that a molecular marker can be developed to identify specific plant growth habits in peanuts, enabling early generation selection by peanut breeders.
文摘Under the background of the all-round deepening of quality education,the cultivation of comprehensive quality has become the main theme of contemporary education reform.Good behavior and habits are of great significance to children’s future learning,growth,and development.Through literature review and other methods,this paper analyzes the current situation of children’s family education and the influence of family education on the cultivation of children’s behavioral habits and provides some strategies for cultivating children’s good behavioral habits in family education.
基金received funding from Science and Technology Department of Zhejiang Province(Grant No.LGF21H170001)the Health Commission of Zhejiang Province(Grant No.2023KY759)Hospital Management soft science research project of Kangenbei in Zhejiang province(2023ZHA-KEB104).
文摘Objective:Cardiovascular disease(CVD)remains a significant public health challenge in China.Accurateperception of individual CVD risk is crucial for timely intervention and preventive strategies.This studyaimed to determine the alignment between CVD risk perception levels and objectively calculated CVDrisk levels,then investigate the disparity in physical activity and healthy diet habits among distinct CVDrisk perception categories.Methods:From March to August 2022,a cross-sectional survey was conducted in Zhejiang Province usingconvenience sampling.Participants aged between 20 and 80 years,without prior diagnosis of CVD wereincluded.CVD risk perception was evaluated with the Chinese version of the Attitude and Beliefs aboutCardiovascular Disease Risk Perception Questionnaire,while objective CVD risk was assessed through thePrediction for Atherosclerotic Cardiovascular Disease Risk(China-PAR)model.Participants’demographicinformation,self-reported physical activity,and healthy diet score were also collected.Results:A total of 739 participants were included in the final analysis.Less than a third of participants(29.2%)accurately perceived their CVD risk,while 64.5%over-perceived it and 6.2%under-perceived it.Notably,half of the individuals(50.0%)with high CVD risk under-perceived their actual risk.Compared tothe accurate perception group,individuals aged 60e80 years old(OR=6.569),currently drinking(OR=3.059),and with hypertension(OR=2.352)were more likely to under-perceive their CVD risk.Meanwhile,participants aged 40-<60 years old(OR=2.462)and employed(OR=2.352)were morelikely to over-perceive their risk.The proportion of individuals engaging in physical activity was lowest inthe under-perception group,although the difference among the three groups was not statistically significant(X^(2)=2.556,P=0.278).In addition,the proportion of individuals practicing healthy diet habitswas also lowest in the under-perception group,and a significant statistical difference was observedamong the three groups(X^(2)=10.310,P=0.006).Conclusion:Only a small proportion of participants accurately perceived their CVD risk,especially amongthose with high actual CVD risk.Individuals in the under-perceived CVD risk group exhibited the lowestrates of physical activity engagement and healthy diet adherence.Healthcare professionals should prioritize implementing personalized CVD risk communication strategies tailored to specific subgroups toenhance the accuracy of risk perception.
基金supported by the Science and Technology Project of State Grid Corporation of China(No.5400202133157A-0-0-00)partially supported by the State Grid Gansu Electric Power Company,China。
文摘Plasma nitrogen fixation(PNF)has been emerging as a promising technology for greenhouse gasfree and renewable energy-based agriculture.Yet,most PNF studies seldom address practical application-specific issues.In this work,we present the development of a compact and automatic PNF system for on-site agricultural applications.The system utilized a gliding-arc discharge as the plasma source and employed a dual-loop design to generate NO_(x)from air and water under atmospheric conditions.Experimental results showed that the system with a dualloop design performs well in terms of energy costs and production rates.Optimal operational parameters for the system were determined through experimentation,resulting in an energy cost of 13.9 MJ mol^(-1)and an energy efficiency of 16 g kWh^(-1)for NO_(3)^(-)production,respectively.Moreover,the concentration of exhausted NO_(x)was below the emission standards.Soilless lettuce cultivation experiments demonstrated that NO_(x)^(-)produced by the PNF system could serve as liquid nitrate nitrogen fertilizer.Overall,our work demonstrates the potential of the developed PNF system for on-site application in the production of green-leaf vegetables.
文摘The possibility of the life origin in the stellar systems, located at a distance of ~200 pc from the solar system, was investigated. The stars, in the spectrums of which C (carbon), O (oxygen), N (nitrogen), and P (phosphorus) are found, are called DNA-stars. Based on stellar abundances a new method for searching for habitable exoplanets has been developed and a list of 48 DNA-stars in the solar neighborhood, on which life is possible, has been defined. The quota of DNA-stars is equal 1.3% of the total amount of Hypatia Stellar Catalog. Only three DNA-stars out of selected 48 stars belong to the spectral class as our Sun (G2V). The closest to the solar system is the DNA-star with the number HIP 15510, which belongs to the G8V class and is 6 pc away from the solar system. Nine DNA-stars, which have the highest chemical similarity with solar spectrum, were identified. It is identified that one of these nine stars, HIP 24681, has six planets.
文摘Appropriate physical exercise has a positive impact on adolescents’physical and mental health,but there is a serious lack of physical exercise among Chinese adolescents.How to shape their exercise behavior(EB)has become an important task in promoting their development.A questionnaire survey was conducted using stratified cluster random sampling on three middle schools by class in Zhejiang Province,China to investigate the impact of exercise atmosphere(EA)on adolescents’exercise behavior and the mediating role of exercise identity(EI)and exercise habit(EH).806 adolescents were investigated by the Exercise Atmosphere Scale(EAS),Exercise-Identity Scale(EIS),Self-Report Habit Index(SRHI),and Physical Activity Rating Scale(PARS-3).The results show that:There is a significant positive correlation between each two of exercise atmosphere,exercise identity,exercise habit,and exercise behavior(p<0.05).Exercise atmosphere could not only directly affect adolescents’physical exercise behavior but can also indirectly affect their physical exercise behavior through the mediating effect of exercise identity and exercise habit,involving three mediating pathways,namely,the mediating path through exercise identity,the mediating pathway through exercise habit and the chain mediating pathway through exercise identity and exercise habit.The direct effect of exercise atmosphere on exercise behavior was 0.459(p<0.01),accounting for 62.62% of the total effect of 0.733,and its indirect effect was 0.274,accounting for 37.28% of the total effect.To a certain extent,the mediating effect model reveals the mechanism of exercise atmosphere affecting exercise behavior and has a certain reference value for promoting adolescents’exercise behavior.We should start by creating an exercise atmosphere,cultivating exercise identity,and enhancing exercise habits to help teenagers form active physical exercise behaviors.
文摘This research advocates for the construction of Climate Change Haven Communities across the Appalachian Region. The proposed development plan can be extended to the northern tier states across the US and also to the northern and mountainous regions of Europe and Asia. We present an analogy to the earlier climate change period of the Last Glacial Maximum/“Ice Age” in which these same northern regions of the planet were covered in ice sheets making them uninhabitable for most humans and many plant and animal species. In some significant ways, the Ice Age scenario can be a reverse-model for our current climate crisis. We also advocate strongly for the prevention of upscale real estate development projects in these same regions of the globe, as these will foreclose the possibility of safely sheltering the millions of persons who will be displaced by climate change over the next 5 to 10 years.
基金supported by the National Key Research and Development Program of China(2023YFD1900802)the China Agriculture Research System of MOF and MARA(CARS-03-19)+2 种基金the National Natural Science Foundation of China(51879267)the Central Public-interest Scientific Institution Basal Research Fund,China(IFI2023-13)the Agricultural Science and Technology Innovation Program(ASTIP),Chinese Academy of Agricultural Sciences。
文摘A high-efficiency mode of high-low seedbed cultivation(HLSC)has been listed as the main agricultural technology to increase land utilization ratio and grain yield in Shandong Province,China.However,limited information is available on the optimized water and nitrogen management for yield formation,especially the grain-filling process,under HLSC mode.A three-year field experiment with four nitrogen rates and three irrigation rates of HLSC was conducted to reveal the response of grain-filling parameters,grain weight percentage of spike weight(GPS),spike moisture content(SMC),and winter wheat yield to water and nitrogen rates.The four nitrogen rates were N1(360 kg ha^(-1) pure N),N2(300 kg ha^(-1) pure N),N3(240 kg ha^(-1) pure N),and N4(180 kg ha^(-1) pure N),respectively,and the three irrigation quotas were W1(120 mm),W2(90 mm),and W3(60 mm),respectively.Results showed that the determinate growth function generally performed well in simulating the temporal dynamics of grain weight(0.989<R^(2)<0.999,where R2 is the determination coefficient).The occurrence time of maximum filling rate(T_(max))and active grain-filling period(AGP)increased with the increase in the water or nitrogen rate,whereas the average grain-filling rate(G_(mean))had a decreasing trend.The final 1,000-grain weight(FTGW)increased and then decreased with the increase in the nitrogen rates and increased with the increase in the irrigation rates.The GPS and SMC had a highly significant quadratic polynomial relationship with grain weight and days after anthesis.Nitrogen,irrigation,and year significantly affected the T_(max),AGP,G_(mean),and FTGW.Particularly,the AGP and FTGW were insignificantly different between high seedbed(HLSC-H)and low seedbed(HLSC-L)across the water and nitrogen levels.Moreover,the moderate water and nitrogen supply was more beneficial for grain yield,as well as for spike number and grain number per hectare.The principal component analysis indicated that combining 240-300 kg N ha^(-1) and 90^(-1)20 mm irrigation quota could improve grain-filling efficiency and yield for the HLSC-cultivated winter wheat.
文摘Strawberry (Fragaria spp.) is one of the most important fruits classified as exotic fruits imported into Cameroon. To have an inventory of its cultivation in Cameroon, a survey study was carried out among eight farms of Fragaria spp. from January 2021 to February 2022. The plant was introduced in Cameroon in 2018. There are 13 varieties of Fragaria spp. currently cultivated. Among these 13 varieties, eleven are hybrids of Fragaria x ananassa (“Amiga”, “Amine”, “Camarosa”, “Chandler”, “Charlotte”, “Elsanta”, “Gariguette”, “Madame Moutot”, “Ostara”, “Ruby gem” and “San Andreas”), and two of the hybrids of Fragaria vesca (“Maestro” and “Mara des bois”). The cropping system, irrigation system, and type of fertilizers applied differ from one strawberry farm to another. Biofertilizers (such as mycorrhizal), inorganic and organic fertilizers are actually used to improve production. The potential annual production of strawberries from January 2021 to February 2022, estimated based on the survey data, was 21.216 tons for all growers. Among these eight production farms, the Lolodorf BIO Farm presents 6000 kg (six tons) of strawberries and 100,000 stolons (seedlings) produced, from seven varieties of Fragaria spp. cultivated, with 6 varieties which are hybrids variety Fragaria x ananassa (“Amiga”, “Amine”, “Chandler”, “Gariguette”, “Madame Moutot”, and “Ruby gem”), and one which is a hybrid of Fragaria vesca (“Mara des bois”). Certain diseases were also observed and recorded depending on the growing areas.
文摘Objective: The cultivation of the innovation ability and scientific research is one of the nursing learning objectives for undergraduate students. To explore the method and effect of training system of scientific research innovation ability of nursing undergraduates based on “3332”. Methods: Three course learning modules are constructed: stage-based course learning module, systematic project practice training module and comprehensive practice training module. A practical training platform for scientific research innovation projects is built, and undergraduate scientific research innovation ability training is carried out from both in-class and out-of-class lines. Results: Since 2017, the students have obtained 7 national innovation and entrepreneurship training programs, 52 university-level undergraduate scientific research projects, published more than 10 academic papers, and obtained 2 patent authorization. Conclusions: The training system of scientific research innovation ability of nursing undergraduates based on “3332” is conducive to the development of scientific research innovation ability of nursing students, and to cultivate nursing talents who can adapt to the development of the new era and have better post competence.
基金supported by the National Key Research and Development Program of China (2022YFD2300304)the National Natural Science Foundation of China (32071944 and 32272197)+2 种基金the Hong Kong Research Grants Council, China (GRF 14177617, 12103219, 12103220, and AoE/M-403/16)the State Key Laboratory of Agrobiotechnology (Strategic Collaborative Projects) in The Chinese University of Hong Kong, China, the Six Talent Peaks Project in Jiangsu Province, China (SWYY151)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD).
文摘Integrative cultivation practices(ICPs)are essential for enhancing cereal yield and resource use efficiency.However,the effects of ICP on the rhizosphere environment and roots of paddy rice are still poorly understood.In this study,four rice varieties were produced in the field.Each variety was treated with six different cultivation techniques,including zero nitrogen application(0 N),local farmers’practice(LFP),nitrogen reduction(NR),and three progressive ICP techniques comprised of enhanced fertilizer N practice and increased plant density(ICP1),a treatment similar to ICP1 but with alternate wetting and moderate drying instead of continuous flooding(ICP2),and the same practices as ICP2 with the application of organic fertilizer(ICP3).The ICPs had greater grain production and nitrogen use efficiency than the other three methods.Root length,dry weight,root diameter,activity of root oxidation,root bleeding rate,zeatin and zeatin riboside compositions,and total organic acids in root exudates were elevated with the introduction of the successive cultivation practices.ICPs enhanced nitrate nitrogen,the activities of urease and invertase,and the diversity of microbes(bacteria)in rhizosphere and non-rhizosphere soil,while reducing the ammonium nitrogen content.The nutrient contents(ammonium nitrogen,total nitrogen,total potassium,total phosphorus,nitrate,and available phosphorus)and urease activity in rhizosphere soil were reduced in all treatments in comparison with the non-rhizosphere soil,but the invertase activity and bacterial diversity were greater.The main root morphology and physiology,and the ammonium nitrogen contents in rhizosphere soil at the primary stages were closely correlated with grain yield and internal nitrogen use efficiency.These findings suggest that the coordinated enhancement of the root system and the environment of the rhizosphere under integrative cultivation approaches may lead to higher rice production.
文摘Polycarbonate plastics containing bisphenol A (BPA) used to manufacture drinking water bottles. Kurdistan region in northern Iraq is a developed area with increased pollution from plastic bottles. Trace amounts of BPA have been detected in bottled water samples. The absorption of BPA was measured with HPLC using a vertical cultivation system with Bulbs of the Allium Cepa plant planted in these plastic bottles with monitored growth. Vertical cultivation was found to have a low level of BPA in the plant cells, making it a safe cultivation method under specific climate conditions. The mean concentration of BPA in vertical cultivation is 0.19 ug/ml (3.8 ng for a 20 uL injection), and the Limit of Quantification (LOQ) is 0.63 ug/ml (12.7 ng for 20 uL injection). While Scanning Electron Microscope (SEM) shows that the concentrations are relatively low in water samples stored at room temperature compared to those exposed to direct sunlight (40°C) and water bottle samples stored at (-4°C), The correlation coefficients were found to be good (0.9992). SEM is used for plastic bottle samples stored at different temperatures. The images identify compound decay and explore the morphology of BPA in manufactured plastic materials.
文摘Tobacco is an essential cash crop in Zimbabwe and a strategic livelihood option for hundreds of thousands of rural households. However, the crop is linked to negative environmental, economic, and social impacts. The existing studies on tobacco cultivation in Zimbabwe present contradictory findings on the determinants and impacts of adoption, leaving unanswered questions about the crop’s sustainability impact in the country. This article investigates the determinants of smallholder farmers’ decisions to grow tobacco and the associated impacts of adoption. Random and purposive sampling were used to select 273 household surveys, including tobacco and non-tobacco smallholder farmers, and 56 expert interviews to answer the research questions. We employed regression models alongside expert interviews and document analysis to identify the determinants influencing the decision-making process of smallholder farmers in Zimbabwe regarding tobacco cultivation. Additionally, our investigation aimed to elucidate the perceived impacts associated with the adoption of this agricultural practice. The regression analysis indicated that the farmer’s age, education level, farming experience, family size, household income, and perceived high farm profitability are significant drivers of tobacco adoption. We also discovered divergent and convergent perceptions of the critical impacts of tobacco cultivation. The study highlights the need for proactive multi-stakeholder collaboration and sustainable financial arrangements to address the negative impacts of tobacco production. As the primary stakeholder responsible for regulating and promoting agricultural activities, the Zimbabwean government should provide meaningful financial support, increase access to credit, and ensure better market facilities for alternative crops to reduce the over-dependence on tobacco.
文摘BACKGROUND Cognitive-behavioral therapy(CBT)and habit reversal training(HRT)have shown application potential in addressing tic symptoms and comorbid psychiatric conditions.Despite their theoretical potential,empirical evidence on their combined efficacy remains limited.AIM To evaluate the efficacy of CBT combined with HRT on anxiety disorders in children with Tourette’s syndrome(TS).METHODS Clinical data of children with TS admitted to our hospital from January 2022 to June 2023 were collected,and the patients were grouped into the conventional therapy(control)group and the CBT combined with HRT group.Baseline charac-teristics,anxiety scores,tic severity scores,treatment adherence,and parental satisfaction were assessed.Statistical analysis was performed using t-tests,chi-square tests,and correlation analysis.RESULTS A total of 136 patients,including 65 patients in the control group and 71 patients in the CBT combined with HRT group,were included.The CBT combined with HRT group showed remarkable improvements compared with the control group.Post-intervention assessment revealed a decrease in anxiety scores from 63.52±1.81 to 40.53±1.64(t=2.022,P=0.045),and the Yale Global Tic Severity Scale total score decreased from 22.14±5.67 to 16.28±4.91(t=2.288,P=0.024).Treatment adherence was significantly higher in the CBT combined with HRT group(85.47±7.62%)compared with the control group(82.32±6.54%;t=2.596,P=0.010).Parental satisfaction scores were also higher in the CBT combined with HRT group(8.69±1.77)compared with the control group(7.87±1.92;t=2.592,P=0.011).CONCLUSION This study demonstrates that CBT combined with HRT significantly reduces anxiety symptoms and tic severity in children with TS,with higher treatment adherence and parental satisfaction.These findings support the potential application of this comprehensive therapeutic approach for TS treatment.
文摘The human being must find a dwelling and protect himself against the vicissitudes of nature.He has to adapt himself to the contemporary world and to his environment.Sometimes,he should accept unwittingly extreme situations dominated by inhabitability and as such abandon his plot of land and properties and to get into the city where he has to refuge into spaces that any human being enjoying a minimum of living conditions would not accept:the slum.For the time being,he does his best to make his environment enjoyable and to transfer his existence into something of a temporary happiness by trying to make order in these inhospitable places in order to create a sort of happiness that he is the only one who can measure its real impact.Throughout this essay,we are trying to present this human habitation that we often meet in Constantine.
基金the Chongqing Talent Plan“Contract System”Project,No.cstc2022ycjh-bgzxm0137Natural Science Foundation of Chongqing,No.CSTB2024NSCQ-MSX0003the Ethics Committee of Chongqing General Hospital.The ethics review number:No.KY S2022-045-01.
文摘BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an important factor in maintaining their long-term expansion,which is essential for shortening the time of drug sensitivity analysis and is the basis of PC research.
基金Supported by Key Scientific Research Project in Colleges and Universities of Henan Province(22B180011)Project of Henan Provincial Department of Science and Technology(232102320262)+1 种基金Education and Teaching Reform Research Project of Pingdingshan University(2021-JY55)Key Demonstration Course of Pingdingshan University in 2022——Comprehensive Experiment of Environmental Biology.
文摘The production environment of greenhouse cultivation is relatively closed,the multiple cropping index is high,the management of fertilizationwatering and pesticideapplication isblindtosomeextent,andthe phenomenonofcontinuous cropping isalsocommonSoilquali-ty affects the sustainable development of greenhouse cultivation.Earthworm is a ubiquitous invertebrate organism in soil,an important part of soil system,a link between terrestrial organisms and soil organisms,an important link in the small cycle of soil material organisms,and plays an important role in maintaining the structure and function of soil ecosystem.Different ecotypes of earthworms are closely related to their habi-tats(soil layers)and food resource preferences,and then affect their ecological functions.The principle of earthworm regulating soil function is essentially the close connection and interaction between earthworm and soil microorganism.Using different ecotypes of earthworms and bio-logical agents to carry out combined remediation of greenhouse cultivation soil is a technical model to realize sustainable development of green-house cultivation.
基金Supported by 2024 Major Facility System Operating Costs of Ministry of Agriculture and Rural Affairs"Ledong Cashew Germplasm Resource Nursery Operating Cost of Ministry of Agriculture and Rural Affairs"2023-2024 Agricultural Germplasm Resource Conservation Project"Research on Collection,Conservation and Utilization of Cashew Germplasm Resources".
文摘In order to enhance the yield and quality of cashew,it is essential to implement high-yield cultivation techniques effectively throughout the production process.Additionally,pest control measures should be employed to provide technical support for the industrialized development of cashew.