Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient co...Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient conditions to help plants cope with recurring environmental fluctuations.Despite the current understanding of plant circadian clock and genetic framework underlying plant shoot architecture,the intricate connection between these two adaptive mechanisms remains largely unclear.In this study,we elucidated how the core clock gene LUX ARRHYTHMO(LUX)regulates shoot architecture in the model legume plant Medicago truncatula.We show that mtlux mutant displays increased main stem height,reduced lateral shoot length,and decreased the number of lateral branches and biomass yield.Gene expression analysis revealed that Mt LUX regulated shoot architecture by repressing the expression of strigolactone receptor MtD14 and MtTB1/MtTCP1A,a TCP gene that functions centrally in modulating shoot architecture.In vivo and in vitro experiments showed that Mt LUX directly binds to a cis-element in the promoter of MtTB1/MtTCP1A,suggesting that Mt LUX regulates branching by rhythmically suppressing MtTB1/MtTCP1A.This work demonstrates the regulatory effect of the circadian clock on shoot architecture,offering a new understanding underlying the genetic basis towards the flexibility of plant shoot architecture.展开更多
In this paper,we report a clock distribution system for Water Cherenkov Detector Arrays(WCDAs) in Large High Altitude Air Shower Observatory(LHAASO) project.The designed electronics system is of high performance in im...In this paper,we report a clock distribution system for Water Cherenkov Detector Arrays(WCDAs) in Large High Altitude Air Shower Observatory(LHAASO) project.The designed electronics system is of high performance in implementing the clock distribution among detectors of a large scale of dimension.Based on Serializer/Deserializer(SerDes) and fiber transmission,the clock distribution system is the modules of central back end to distributed front end.The clock distribution system has been evaluated with a two modules system.While all the four SerDes candidates for clock transmission with jitters below 17 ps,the DS92LV16 has a fixed phase relationship between transmission clock and recovered clock,hence its use in LHAASO WCDAs.展开更多
With its advantages in wirelength reduction and routing flexibility compared with conventional Manhattan routing, X architecture has been proposed and applied to modern IC design. As a critical part in high-performanc...With its advantages in wirelength reduction and routing flexibility compared with conventional Manhattan routing, X architecture has been proposed and applied to modern IC design. As a critical part in high-performance integrated circuits, clock network design meets great challenges due to feature size decrease and clock frequency increase. In order to eliminate the delay and attenuation of clock signal introduced by the vias, and to make it more tolerant to process variations, in this paper, we propose an algorithm of a single layer zero skew clock routing in X architecture （called Pianar-CRX）. Our Planar- CRX method integrates the extended deferred-merge embedding algorithm （DME-X, which extends the DME algorithm to X architecture） with modified Ohtsuki＇s line-search algorithm to minimize the total wirelength and the bends. Compared with planar clock routing in the Manhattan plane, our method achieves a reduction of 6.81% in total wirelength on average and gets the resultant clock tree with fewer bends. Experimental results also indicate that our solution can be comparable with previous non-planar zero skew clock routing algorithm.展开更多
基金supported by Laboratory of Lingnan Modern Agriculture Project(NZ2021001)State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources(SKICUSA-a202007)Natural Science Foundation of Guangdong Province(2022A1515011027,2019A1515012009)。
文摘Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient conditions to help plants cope with recurring environmental fluctuations.Despite the current understanding of plant circadian clock and genetic framework underlying plant shoot architecture,the intricate connection between these two adaptive mechanisms remains largely unclear.In this study,we elucidated how the core clock gene LUX ARRHYTHMO(LUX)regulates shoot architecture in the model legume plant Medicago truncatula.We show that mtlux mutant displays increased main stem height,reduced lateral shoot length,and decreased the number of lateral branches and biomass yield.Gene expression analysis revealed that Mt LUX regulated shoot architecture by repressing the expression of strigolactone receptor MtD14 and MtTB1/MtTCP1A,a TCP gene that functions centrally in modulating shoot architecture.In vivo and in vitro experiments showed that Mt LUX directly binds to a cis-element in the promoter of MtTB1/MtTCP1A,suggesting that Mt LUX regulates branching by rhythmically suppressing MtTB1/MtTCP1A.This work demonstrates the regulatory effect of the circadian clock on shoot architecture,offering a new understanding underlying the genetic basis towards the flexibility of plant shoot architecture.
基金Supported by the Knowledge Innovation Program of Chinese Academy of Sciences(Grant No.KJCX2-YW-N31)
文摘In this paper,we report a clock distribution system for Water Cherenkov Detector Arrays(WCDAs) in Large High Altitude Air Shower Observatory(LHAASO) project.The designed electronics system is of high performance in implementing the clock distribution among detectors of a large scale of dimension.Based on Serializer/Deserializer(SerDes) and fiber transmission,the clock distribution system is the modules of central back end to distributed front end.The clock distribution system has been evaluated with a two modules system.While all the four SerDes candidates for clock transmission with jitters below 17 ps,the DS92LV16 has a fixed phase relationship between transmission clock and recovered clock,hence its use in LHAASO WCDAs.
基金Supported in part by the National Natural Science Foundation of China (Grant No. 60876026), the Specialized Research Fund for the Doctoral Program of Higher Education (Crant No. 200800030026)
文摘With its advantages in wirelength reduction and routing flexibility compared with conventional Manhattan routing, X architecture has been proposed and applied to modern IC design. As a critical part in high-performance integrated circuits, clock network design meets great challenges due to feature size decrease and clock frequency increase. In order to eliminate the delay and attenuation of clock signal introduced by the vias, and to make it more tolerant to process variations, in this paper, we propose an algorithm of a single layer zero skew clock routing in X architecture （called Pianar-CRX）. Our Planar- CRX method integrates the extended deferred-merge embedding algorithm （DME-X, which extends the DME algorithm to X architecture） with modified Ohtsuki＇s line-search algorithm to minimize the total wirelength and the bends. Compared with planar clock routing in the Manhattan plane, our method achieves a reduction of 6.81% in total wirelength on average and gets the resultant clock tree with fewer bends. Experimental results also indicate that our solution can be comparable with previous non-planar zero skew clock routing algorithm.
