Objective:To investigate the protective role of Cardiospermum halicacabum(C.halicacabum) leaf extract on glycoprotein metabolism in streptozotocin(STZ)-induced diabetic rats.Methods: Diabetes was induced in male albin...Objective:To investigate the protective role of Cardiospermum halicacabum(C.halicacabum) leaf extract on glycoprotein metabolism in streptozotocin(STZ)-induced diabetic rats.Methods: Diabetes was induced in male albino Wistar rats by intraperitonial administration of STZ.The C.halicacabum leaf extract(CHE) was administered orally to normal and STZ-diabetic rats for 45 days.The effects of C.halicacabum leaf extract(CHE) on plasma and tissue glycoproteins (hexose,hexosamine,fucose and sialic acid) were determined.Results:The levels of plasma and tissues glycoproteins containing hexose,hexosamine and fucose were significantly increased in STZ-induced diabetic rats.In addition,the level of sialic acid significantly increased in plasma and liver while decreased in kidney of STZ-induced diabetic rats.After administration of CHE to diabetic rats,the metabolic alteration of glycoprotein reverted towards normal levels. Conclusions:The present study indicates that the CHE possesses a protective effect on abnormal glycoprotein metabolism in addition to its antihyperglycemic activity.展开更多
Objective:To determine repellent activity of hexane,ethyl acetate,benzene,chloroform and methanol extract of Cardiospermum halicacabum,(C.halicacabum) against Culex quinque/ascialus(Cx.quinquefuscialus),Aedes aegypti(...Objective:To determine repellent activity of hexane,ethyl acetate,benzene,chloroform and methanol extract of Cardiospermum halicacabum,(C.halicacabum) against Culex quinque/ascialus(Cx.quinquefuscialus),Aedes aegypti(Ae.aegypli) and Anopheles slephensi(An.slephensi). Methods:Evaluation was carried out in a net cage(45 cm×30 cm×25 cm) containing 100 blood starved female mosquitoes of three mosquito species and were assayed in the laboratory condition by using the protocol of WHO 2005;The plant leaf crude extracts of C.halicacabum was applied at 1.0,2.5,and 5.0 me/cm^2 separately in the exposed area of the fore arm.Only ethanol served as control.Results:In this observation,the plant crude extracts gave protection against mosquito bites without any allergic reaction to the test person,and also,the repellent activity was dependent on the strength of the plant extracts.The tested plant crude extracts had exerted promising repellent against all the three mosquitoes.Conclusions:From the results it can be concluded the crude extract of C.halicacabum was potential for controlling Cx.quinquefasciatus, Ae.aegypli and An.slephensi mosquitoes.展开更多
The present study aimed to evaluate the effects of chrysoeriol from Cardiospermum halicacabum in streptozotocin induced Wistar rats.Thirty rats were categorized as control,diabetic control supplemented with 0,20 mg/kg...The present study aimed to evaluate the effects of chrysoeriol from Cardiospermum halicacabum in streptozotocin induced Wistar rats.Thirty rats were categorized as control,diabetic control supplemented with 0,20 mg/kg chrysoeriol and 600μg/kg BW of glibenclamide for 45-day trial period.Our results indicated that the inclusion of chrysoeriol(20 mg/kg)showed a significant reduction in plasma glucose,hemoglobin and glycosylated hemoglobin level with a rising of plasma insulin sensitivity.Further,downregulated enzymes including glucose 6-phosphatase,fructose 1,6-bisphosphatase,and glycogen phosphorylase as well upregulated enzymes such as hexokinase,glucose-6-phosphate dehydrogenase,pyruvate kinase,and hepatic glycogen content.There was a diminish action found in liver glycogen synthase of tested rat with a rise in gamma-glutamyl transpeptidase,towards normal levels upon treatment with chrysoeriol.The histopathological study confirmed that renewal of the beta cells of pancreatic of chrysoeriol and glibenclamide treated rats.In addition,the molecular docking of chrysoeriol against glycolytic enzymes including hexokinase,glucose-6-phosphate dehydrogenase,pyruvate kinase,using Argus software shows chrysoeriol had greatest ligand binding energy as equivalent to glibenclamide,as a standard drug.Thus,chrysoeriol found to be non-toxic with potential regulation on glycemic control and upregulation of the carbohydrate metabolic enzymes.展开更多
基金provided from the National Nutrition Policy Chair,King Saud University,Riyadh,Saudi Arabia
文摘Objective:To investigate the protective role of Cardiospermum halicacabum(C.halicacabum) leaf extract on glycoprotein metabolism in streptozotocin(STZ)-induced diabetic rats.Methods: Diabetes was induced in male albino Wistar rats by intraperitonial administration of STZ.The C.halicacabum leaf extract(CHE) was administered orally to normal and STZ-diabetic rats for 45 days.The effects of C.halicacabum leaf extract(CHE) on plasma and tissue glycoproteins (hexose,hexosamine,fucose and sialic acid) were determined.Results:The levels of plasma and tissues glycoproteins containing hexose,hexosamine and fucose were significantly increased in STZ-induced diabetic rats.In addition,the level of sialic acid significantly increased in plasma and liver while decreased in kidney of STZ-induced diabetic rats.After administration of CHE to diabetic rats,the metabolic alteration of glycoprotein reverted towards normal levels. Conclusions:The present study indicates that the CHE possesses a protective effect on abnormal glycoprotein metabolism in addition to its antihyperglycemic activity.
基金supported by Department of Science and Technology(DST.SERC-Fast Track Young Scientist Project-No.SR/FT/LS-072/2008).New Delhi.India
文摘Objective:To determine repellent activity of hexane,ethyl acetate,benzene,chloroform and methanol extract of Cardiospermum halicacabum,(C.halicacabum) against Culex quinque/ascialus(Cx.quinquefuscialus),Aedes aegypti(Ae.aegypli) and Anopheles slephensi(An.slephensi). Methods:Evaluation was carried out in a net cage(45 cm×30 cm×25 cm) containing 100 blood starved female mosquitoes of three mosquito species and were assayed in the laboratory condition by using the protocol of WHO 2005;The plant leaf crude extracts of C.halicacabum was applied at 1.0,2.5,and 5.0 me/cm^2 separately in the exposed area of the fore arm.Only ethanol served as control.Results:In this observation,the plant crude extracts gave protection against mosquito bites without any allergic reaction to the test person,and also,the repellent activity was dependent on the strength of the plant extracts.The tested plant crude extracts had exerted promising repellent against all the three mosquitoes.Conclusions:From the results it can be concluded the crude extract of C.halicacabum was potential for controlling Cx.quinquefasciatus, Ae.aegypli and An.slephensi mosquitoes.
文摘The present study aimed to evaluate the effects of chrysoeriol from Cardiospermum halicacabum in streptozotocin induced Wistar rats.Thirty rats were categorized as control,diabetic control supplemented with 0,20 mg/kg chrysoeriol and 600μg/kg BW of glibenclamide for 45-day trial period.Our results indicated that the inclusion of chrysoeriol(20 mg/kg)showed a significant reduction in plasma glucose,hemoglobin and glycosylated hemoglobin level with a rising of plasma insulin sensitivity.Further,downregulated enzymes including glucose 6-phosphatase,fructose 1,6-bisphosphatase,and glycogen phosphorylase as well upregulated enzymes such as hexokinase,glucose-6-phosphate dehydrogenase,pyruvate kinase,and hepatic glycogen content.There was a diminish action found in liver glycogen synthase of tested rat with a rise in gamma-glutamyl transpeptidase,towards normal levels upon treatment with chrysoeriol.The histopathological study confirmed that renewal of the beta cells of pancreatic of chrysoeriol and glibenclamide treated rats.In addition,the molecular docking of chrysoeriol against glycolytic enzymes including hexokinase,glucose-6-phosphate dehydrogenase,pyruvate kinase,using Argus software shows chrysoeriol had greatest ligand binding energy as equivalent to glibenclamide,as a standard drug.Thus,chrysoeriol found to be non-toxic with potential regulation on glycemic control and upregulation of the carbohydrate metabolic enzymes.
