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Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding 被引量:43
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作者 Baobao Wang Lei Zhu +6 位作者 Binbin Zhao Yongping Zhao Yurong Xie Zhigang Zheng Yaoyao Li Juan Sun Haiyang Wang 《Molecular Plant》 SCIE CAS CSCD 2019年第4期597-602,共6页
Crop breeding aims to generate pure in bred lines with multiple desired traits. Doubled haploid (DH) and genome editing using CRISPR/Cas9 are two powerful game-changing technologies in crop breeding. However, both of ... Crop breeding aims to generate pure in bred lines with multiple desired traits. Doubled haploid (DH) and genome editing using CRISPR/Cas9 are two powerful game-changing technologies in crop breeding. However, both of them still fall short for rapid generation of pure elite lines with integrated favorable traits. Here, we report the development of a Haploid-Inducer Mediated Genome Editing (IMGE) approach, which utilizes a maize haploid inducer line carrying a CRISPR/Cas9 cassette targeting for a desired agronomic trait to pollinate an elite maize in bred line and to generate genome-edited haploids in the elite maize background. Homozygous pure DH lines with the desired trait improvement could be generated within two generations, thus bypassing the lengthy procedure of repeated crossing and backcrossing used in conventional breeding for integrating a desirable trait into elite commercial backgrounds. 展开更多
关键词 maize doubled HAPLOIDS (DH) CRSIPR/Cas9 haploid-inducer mediated genome EDITING (IMGE) crop breeding
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