Esext3 for Reducing the Effect of Harboring Bug in File System

One of the most critical threats to the reliability and robustness for file system is harboring bug (silent data corruption). In this research we focus on checksum mismatch since it occurs not only in the user data but also in file system. Our proposed solution has the ability to check this bug in file system of Linux. In our proposed solution there is no need to invoke or revoke checker utility, it comes as the integrated part of file system and has the ability to check upcoming updates before harboring bug make unrecoverable changes that leads significant data loses. Demonstration testing shows satisfactory results in file server and web server environments in terms of less memory consumption and avoidable delay in system’s updating.

A phase II study on Mefatinib as first-line treatment of patients with advanced non-small-cell lung cancer harboring uncommon EGFR mutations

Dear Editor,Uncommon mutations in exons 18-21 of the epidermal growth factor receptor(EGFR)gene account for 10%–15%of all EGFR mutations when considered as a whole group[1,2].However,each variant confers heterogeneous clinical outcomes to different generations of EGFR tyrosine kinase inhibitors(TKIs)with G719X,L861Q,and/or S768I showing adequate sensitivity to EGFR inhibition[1–3].Osimertinib,based on its superior survival outcomes,has become the preferred first-line treatment for patients diagnosed with advanced non-small cell lung cancer(NSCLC)harboring common EGFR mutations[4];however,its efficacy in patients harboring G719X,S768I,and/or L861Q mutations was comparable or even inferior to Afatinib[5].Afatinib,a second-generation EGFR-TKI,has received approval for extended clinical indication in treating previously untreated patients with metastatic NSCLC harboring G719X,L861Q,and/or S768I based on the findings from the pooled analysis of three clinical trials(LUX-Lung 2/3/6)[2].The real-world clinical efficacy of Afatinib for treating this patient subset has been consistently demonstrated by two large retrospective studies[6,7].In China,chemotherapy remains a standard first-line treatment for this patient subset,with Afatinib available only as an off-label treatment option.

Obituary:Prof.Yun Zhang(1963-2023)-A scientist focused on toxins and their underlying mechanisms to decipher human diseases

Prof.Yun Zhang was born on 9 July 1963 in Kunming,Yunnan,China,during a tumultuous period which he often referenced.Throughout his life,he harbored a steadfast belief in using knowledge to unravel the mysteries of human diseases.His educational journey was marked by frequent changes in schools due to his parents’occupational relocations.However,despite these challenges,he consistently displayed diligence and was admitted to the East China University of Science and Technology,Shanghai,after completing high school in 1980.He remained an active and loyal member of the School of Biotechnology at the university.

Gesang Pedron,the First Female Tibetan Air Force Pilot

On the momentous occasion of the 60th anniversary of the People's Republic of China in 2009,the inaugural group of female Air Force pilots joined their male counterparts to form the Chinese Air Force Echelon,soaring over Tian'anmen Square.Their aerobatic displays captivated the nation, earning widespread admiration for their heroic demeanor,courageous spirit,and striking presence.This spectacle of confidence and youthful elegance inspired countless young girls,including a Tibetan teenager from the distant snowy plateau.Dreaming of serving her country,she harbored aspirations to one day turn these dreams into reality and see the seeds of her ambition blossom.In this new era,these intrepid female pilots patrol the azure boundaries of the sea,guard the skies above the motherland,and stand watch over the snowy highland.

Mosaic isodicentric Y chromosome harboring intact AZF region in a cryptozoospermic male with normal hormone levels

Dear Editor,Structural aberrations of the Y chromosome,including deletions,ring chromosomes,Y-autosomal or Y-X translocations,isochromosomes,and dicentrics,occur in approximately 0.2%of live births;1 among these,partial deletion in the azoospermia factor(AZF)region of the Y chromosome is a well-characterized cause of nonobstructive severe oligozoospermia and azoospermia.2 Meanwhile,a dicentric Y chromosome,abbreviated as dic(Y),was observed to be exclusively present in males with azoospermia,suggesting more severe negative consequences of the AZF deletion for spermatogenesis.

High-throughput sequencing detection and ensartinib treatment of lung cancer harboring NTRK1 fusion

Dear Editor,Although fusion events involving neurotrophic receptor tyrosine kinase 1,2,and 3 genes(NTRK1,NTRK2,and NTRK3,encoding TRKA/B/C respectively)were found in diverse tumor types,only 0.1%-0.3%of lung cancer patients harbor an NTRK(and mostly NTRK1)fusion as the primary oncogenic event[1].Such low prevalence may be partially due to the limited availability of first-line assays for detecting rare fusion events[2].Immunohistochemistry is limited by sensitivity and variable tissue background,and fluorescence in-situ hybridization falls short of elucidating functional significance such as the identity of the partner or structure of the transcript.While DNA next-generation sequencing(NGS)is suitable for mutation calling(single nucleotide variation[SNV]and insertion-or-deletion[indel]),and RNA NGS is particularly effective in detecting fusions[3],routinely performing these two assays together is labor-intensive.As a solution,we have developed a single NGS assay(PANO-Seq)for unified RNA/DNA target enrichment library preparation[4],which takes about 12 hours and $10 to prepare.

Numerical Investigations on Harbor Oscillations Induced by Falling Objects

In this paper,the open-sourced computational fluid dynamics software,OpenFOAM~?,is used to study the fluctuation phenomenon of the water body inside a horizontally one-dimensional enclosed harbor basin with constant water depth triggered by falling wedges with various horizontal falling positions,initial falling velocities and masses.Based on both Fourier transfo rm analysis and wavelet spectrum analysis for the time series of the free surface elevations inside the harbor basin,it is found for the first time that the wedge falling inside the harbor can directly trigger harbor resonance.The influences of the three factors(including the horizontal falling position,the initial falling velocity,and the mass)on the response amplitudes of the lowest three resonant modes are also investigated.The results show that when the wedge falls on one of the nodal points of a resonant mode,the mode would be remarkably suppressed.Conversely,when the wedge falls on one of the anti-nodal points of a resonant mode,the mode would be evidently triggered.The initial falling velocity of the wedge mainly has a remarkable effect on the response amplitude of the most significant mode,and the latter shows a gradual increase trend with the increase of the former.While for the other two less significant modes,their response amplitudes fluctuate around certain constant values as the initial falling velocity rises.In general,the response amplitudes of all the lowest three modes are shown to gradually increase with the mass of the wedge.

Tiger reappearance in Medog highlights the conservation values of the region for this apex predator

In less than a century,Asia’s largest apex predator,the tiger(Panthera tigris),has been relegated to isolated populations surviving in only a small fraction of its historical range.The Medog region,located in Xizang Zizhiqu(Tibet),China,is an important stronghold for this ecologically important apex predator.To enhance our knowledge of the status of tigers in the Medog region,we carried out a systematic camera trapping survey combined with socioecological data collection between April 2020 and May 2022.We surveyed a 1769 km2 area across the Medog region,with a total valid sampling effort of 43163 camera days from 322 camera stations.We also collected socioecological data from 27 forest rangers.We documented tigers at eight camera trapping stations at two villages separated by the Jinzhu Tsangpo River,confirming the presence of at least one adult male individual within the Medog region.These new records were observed over 80 km northeast of the first record in 2019.Additionally,residents reported tiger sightings in 2020 and likely tiger pugmarks in 2021.These spatially and temporally separate records of tigers in the Medog region provide valuable data for informing species-oriented conservation management and highlight the importance of the region for tiger movement and population expansion.However,although encouraging,our survey also detected human activities that may threaten tigers and their potential prey.Ensuring the future survival of tigers in the Medog region entails anti-poaching patrols and long-term camera trapping efforts.In addition,it is also important to identify and establish corridors that connect the Medog region with the adjacent Zangnan region,which also harbors an important population of tigers.

The collagen type Ⅰ alpha 1 chain gene is an alternative safe harbor locus in the porcine genome

Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of bioreactors.Safe harbor loci in the animal genome enable consistent overexpression of foreign genes,without side effects.However,relatively few safe harbor loci are available in pigs,a fact which has impeded the development of multi-transgenic pig research.We report a strategy for efficient transgene knock-in in the endogenous collagen type I alpha 1 chain(COL1A1)gene using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)system.After the knock-in of a 2A peptide-green fluorescence protein(2A-GFP)transgene in the last codon of COL1A1 in multiple porcine cells,including porcine kidney epithelial(PK15),porcine embryonic fibroblast(PEF)and porcine intestinal epithelial(IPI-2I)cells,quantitative PCR(qPCR),Western blotting,RNA-seq and CCK8 assay were performed to assess the safety of COL1A1 locus.The qPCR results showed that the GFP knock-in had no effect(P=0.29,P=0.66 and P=0.20 for PK15,PEF and IPI-2I cells,respectively)on the mRNA expression of COL1A1 gene.Similarly,no significant differences(P=0.64,P=0.48 and P=0.80 for PK15,PEF and IPI-2I cells,respectively)were found between the GFP knock-in and wild type cells by Western blotting.RNA-seq results revealed that the transcriptome of GFP knock-in PEF cells had a significant positive correlation(P<2.2e–16)with that of the wild type cells,indicating that the GFP knock-in did not alter the global expression of endogenous genes.Furthermore,the CCK8 assay showed that the GFP knock-in events had no adverse effects(P_(24)h=0.31,P_(48)h=0.96,P_(72)h=0.24,P_(96)h=0.17,and P_(120)h=0.38)on cell proliferation of PK15 cells.These results indicate that the COL1A1 locus can be used as a safe harbor for foreign genes knock-in into the pig genome and can be broadly applied to farm animal breeding and biomedical model establishment.

Mathematical Modeling of Moored Ship Motion in Arbitrary Harbor utilizing the Porous Breakwater

The motion of the moored ship in the harbor is a classical hydrodynamics problem that still faces many challenges in naval operations,such as cargo transfer and ship pairings between a big transport ship and some small ships.A mathematical model is presented based on the Laplace equation utilizing the porous breakwater to investigate the moored ship motion in a partially absorbing/reflecting harbor.The motion of the moored ship is described with the hydrodynamic forces along the rotational motion(roll,pitch,and yaw)and translational motion(surge,sway,and heave).The efficiency of the numerical method is verified by comparing it with the analytical study of Yu and Chwang(1994)for the porous breakwater,and the moored ship motion is compared with the theoretical and experimental data obtained by Yoo(1998)and Takagi et al.(1993).Further,the current numerical scheme is implemented on the realistic Visakhapatnam Fishing port,India,in order to analyze the hydrodynamic forces on moored ship motion under resonance conditions.The model incorporates some essential strategies such as adding a porous breakwater and utilizing the wave absorber to reduce the port’s resonance.It has been observed that these tactics have a significant impact on the resonance inside the port for safe maritime navigation.Therefore,the current numerical model provides an efficient tool to reduce the resonance within the arbitrarily shaped ports for secure anchoring.

Strong Signals

The sixth edition of the China International Import Expo(CIIE)was held in Shanghai in early November.This was the first time the global economic and trade event was fully resumed offline since the COVID-19 pandemic,and it welcomed a record number of exhibitors.Joining the consistent enthusiasm from ASEAN countries,the United States sent official representatives in the“strongestever delegation”to the CIIE,headed by Jason Hafemeister,acting deputy under-secretary for trade and foreign agricultural affairs for the U.S.Department of Agriculture.All parties considered this year’s event particularly important and harbored high hopes for its ability to enhance trade cooperation with China.

Cultural Symbols in Dense Forests A mysterious ancient capital is glowing with new vitality

In 1861,French biologist Henri Mouhot was searching for tropical animals in the dense forests of Siem Reap,Cambodia,when he inadvertently happened upon a mysterious and magnificent ancient architectural complex harboring the secrets of the long-lost Angkor civilization.Angkor archaeological site,one of the“Four Wonders of the East”together with the Pyramids of Egypt,the Great Wall of China and the Borobudur of Indonesia,is regarded by Cambodian people as a cultural symbol of the national spirit.Since the country’s independence in 1953,the Cambodian national flag features a depiction of Angkor Wat.In 1992,the Angkor archaeological site was placed on UNESCO’s List of World Heritage in Danger.

海豹研究,面部识别技术来帮忙

美国纽约州科尔盖特大学的研究人员开发出了SealNet系统。科学家们认为他们发现了面部识别技术的一个新用途--拯救海豹。Scientists believe they have found a new use for facial recognition technology:saving large ocean animals known as seals.Researchers at Colgate University in the US state of New York have developed SealNet.The system is a database of seal faces created by taking pictures of many harbor seals in Maine􀆳s Casco Bay.

Distribution and ecological evaluation of heavy metals in multi-medium of the Dongzhai Harbor

The distribution characteristics of heavy metals such as Cu, Zn, Pb, Cd, Hg. As in seawater, superficail sediments and several marine organisms of Dongzhai Harbor in Hainan were analyzed. Index methods of the single factor and geo-accumulation were used to assess the pollution degree of heavy metals. The results showed that the heavy metal levels in the seawater of Dongzhai Harbor accorded with the first category of the seawater except Pb, and were much lower than the fishery water quality standard; the concentration of the heavy metal in surface sediments did not exceed the national benchmark of the first-class of marine sediment quality basically, and the superficial sediments were essentially uncontaminated; the heavy metal content in wetland sediments was slightly higher than those in other typical mangrove wetland island of Hainan and marine sediments in Dongzhai Haibor, which indicated this area might has been affected by land-based pollution influence, and this should cause the attention of related departments; the heavy metal concentrations in marine organisms were up to the first category. All were not beyond the standard of non-environmental pollution aquatic products.

Source analysis and ecological risk assessment for heavy metals in the sediments of the Yangshan Deepwater Harbor in autumn and winter

Temporal and spatial variations of concentrations of heavy metals including mercury （Hg）, zinc （Zn）, lead （Pb）, arsenic （As）, copper （Cu）, and cadmium （Cd） in the sediments of the Yangshan Deepwater Harbor were determined based on 6 cruises in autumn and winter, respectively, from 2010 to 2013. The results demonstrated that the overall concentrations of heavy metals were low and distributed in uniform patterns. The concentrations of Hg, Zn, Pb, and Cd in autumn were significantly higher than those in winter with small fluctuations for As and Cu in terms of seasonal variations. Results of factor analysis showed that Pb, Cd, and Zn were derived from inland industrial and shipping discharges as well as the degradation of organic pollutants in marine environment. While agricultural pollutions, cargo shifting and construction debris from reclamation projects contributed to the sources of Cu, As, and Hg. Ecological risk assessment by Mean Sediment Quality Guideline Quotient （SQG-Q） revealed that the degree for eco-risk of the sediments was low-and-moderate in autumn, higher than that in winter. Hg and Cu were the dominant eco-risk factors. The results of Index of geoaccumulation （Igeo） showed that the whole sites of the sea area were barely influenced by Hg, As, Zn, and Pb, and the extents of Cd and Cu contaminations were in low grade. Contamination degree of all the six heavy metals could be ranked as the following： Cd〉Pb〉Zn〉Hg〉As. According to the results of integrated score of factor analysis, the contamination degree for heavy metals in sediments of the Yangshan Deepwater Harbor was low, despite sites No.5, No.4 and No.3, which were heavily contaminated compared with others.

匿名化服务在区域卫生信息平台中的应用研究

为了解决患者数据越来越多地应用到临床诊疗服务、教学、临床科研和公共卫生等领域遭到隐私泄露的问题,采用基于HIPAA法案的Safe Harbor方法,设计研究一种灵活可配置的以CDA文档为输入输出的匿名化服务为解决匿名化的解决方案,达到区域卫生信息平台信息的隐私保护的结果。最终结论研究实现以匿名化服务,结合数据共享协议的使用,实现区域卫生信息平台上患者信息的隐私保护。

基于Harbor实现Docker私有仓库搭建的研究

随着Docker容器技术的广泛应用,基于安全角度考虑,越来越多的企业需要搭建自己的Docker私有仓库,以方便对私有镜像的管理。Docker私有仓库的搭建可采用Registry和Harbor两种方式。在对比两种搭建方式的基础上,主要对Harbor的整体架构和实现过程进行介绍,并应用案例加以实现。

企业私有云容器技术的高可用性研究

云计算技术的成熟,使得人们已经进入了云计算3.0时代,以云原生和重构业务为主要特征的云计算时代更聚焦于应用的云化和敏捷开发,主要的代表技术容器技术使得云计算的3.0时代成为新一代互联网的发展趋势。该文重点研究了容器技术Docker技术的镜像管理中心Harbor的后台存储的高可用性,使得Harbor的后台存储更加的高效敏捷和安全。

基于Harbor搭建高可用Docker私有仓库的研究与实现

随着容器技术的广泛应用,越来越多的大中型企业在生产环境中通过Harbor部署Docker私有仓库。从高可用角度考虑,如何解决在生产环境中因出现单点故障从而对项目开发造成影响并造成经济损失的问题变得尤为重要。该文通过对比三种Harbor高可用性方案后,对基于共享服务的Harbor同步方案进行实现。

Effect of Development of Caofeidian Harbor Area in Bohai Bay on Hydrodynamic Sediment Environment

Based on the characteristics of waves, tidal currents, sediment and seabed evolution in the Caofeidian sea area in the Bohai Bay, a 2D sediment mathematical model of waves and tidal currents is employed to study the development schemes of the harbor. Verification of spring and neap tidal currents and sediment in the winter and summer of 2006 shows that the calculated values of tidal stages as well as flow velocities, flow directions and sediment concentration of 15 synchronous vertical lines are in good agreement with the measured data. Also, deposition and erosion of the sea area in front of Caofeidian ore terminal induced by suspended load under tidal currents and waves are verified; it shows that the calculated values of depth of deposition and erosion as well as their distribution are close to the measured data. Furthermore, effects of reclamation scheme of island in front of the land behind Caofeidian harbor on the hydrodynamic environment are studied, including changes of flow velocities in the deep channels at the south side of Caofeidian foreland and Laolonggou and in various harbor basins, as well as changes of deposition and erosion of seabed induced by the project.