Harpagoside, which is one main iridoid constituent of the dried roots of Scrophularia ningpoensis Hemsl., was biotransformated by bacteria isolated from human feeal flora a.d three metabolites were obtained. The struc...Harpagoside, which is one main iridoid constituent of the dried roots of Scrophularia ningpoensis Hemsl., was biotransformated by bacteria isolated from human feeal flora a.d three metabolites were obtained. The structures of the metabolites, including two new alkaloids, named bar'Pagometabolins Ⅰ (l) and Ⅱ (2), and a known alkaloid acubinine B(3), were identified by chemical methods and the spectroscopic evidences.展开更多
目的建立基于多种内参物的一测多评法测定金莲清热颗粒中芒果苷、荭草素-2″-O-β-L-半乳糖苷、荭草苷、藜芦酸、牡荆苷和哈巴俄苷6个成分的含量。方法以Agilent Eclipse Plus C_(18)为色谱柱,以乙腈-0.1%磷酸溶液为流动相(梯度洗脱),...目的建立基于多种内参物的一测多评法测定金莲清热颗粒中芒果苷、荭草素-2″-O-β-L-半乳糖苷、荭草苷、藜芦酸、牡荆苷和哈巴俄苷6个成分的含量。方法以Agilent Eclipse Plus C_(18)为色谱柱,以乙腈-0.1%磷酸溶液为流动相(梯度洗脱),流速为1mL/min,柱温为30℃,检测波长为270 nm。分别以荭草苷、牡荆苷、荭草素-2″-O-β-L-半乳糖苷为内参物,采用一测多评法建立另外5个待测成分与内参物的相对校正因子,然后计算21批金莲清热颗粒样品中6个成分的含量,并与外标法测定结果进行比较。结果一测多评法测得21批金莲清热颗粒样品中芒果苷、荭草素-2″-O-β-L-半乳糖苷、荭草苷、藜芦酸、牡荆苷和哈巴俄苷的含量范围分别为0.234~0.516、1.804~2.270、2.143~2.606、0.190~0.223、0.594~0.782、0.080~0.152 mg/g,外标法测定结果分别为0.235~0.523、1.798~2.265、2.137~2.599、0.190~0.224、0.597~0.786、0.077~0.151 mg/g,2种方法所测结果的差异百分比不大于4.00%。结论建立了基于多种内参物同时测定金莲清热颗粒中6种成分含量的一测多评法,该方法与外标法所得结果无明显差异,可用于金莲清热颗粒的质量控制。展开更多
基金This work is supported by the ''Ninth 5-year Plan" Key Science Technique R & D Programme Foundation of China !(96-C02-03-06)
文摘Harpagoside, which is one main iridoid constituent of the dried roots of Scrophularia ningpoensis Hemsl., was biotransformated by bacteria isolated from human feeal flora a.d three metabolites were obtained. The structures of the metabolites, including two new alkaloids, named bar'Pagometabolins Ⅰ (l) and Ⅱ (2), and a known alkaloid acubinine B(3), were identified by chemical methods and the spectroscopic evidences.