Natural polyphenols effects on protein aggregates in Alzheimer＇s and Parkinson＇s prion-like diseases

Alzheimer＇s and Parkinson＇s diseases are the most common neurodegenerative diseases. They are characterized by protein aggregates and so can be considered as prion-like disease. The major components of these deposits are amyloid peptide and tau for Alzheimer＇s disease, α-synuclein and synphilin-1 for Parkinson＇s disease. Drugs currently proposed to treat these pathologies do not prevent neurodegenerative processes and are mainly symptomatic therapies. Molecules inducing inhibition of aggregation or disaggregation of these proteins could have beneficial effects, especially if they have other beneficial effects for these diseases. Thus, several natural polyphenols, which have antioxidative, anti-inflammatory and neuroprotective properties, have been largely studied, for their effects on protein aggregates found in these diseases, notably in vitro. In this article, we propose to review the significant papers concerning the role of polyphenols on aggregation and disaggregation of amyloid peptide, tau, α-synuclein, synphilin-1, suggesting that these compounds could be useful in the treatments in Alzheimer＇s and Parkinson＇s diseases.

Polyphenols as potential enhancers of stem cell therapy against neurodegeneration

The potential of polyphenols for treating chronic-degenerative diseases(particularly neurodegenerative diseases)is attractive.However,the selection of the best polyphenol for each treatment,the mechanisms by which they act,and their efficacy are frequently discussed.In this review,the basics and the advances in the field,as well as suggestions for using natural and synthetic polyphenols alone or in a combinatorial strategy with stem cell assays,are compiled and discussed.Thus,stem cells exhibit several responses when polyphenols are added to their environment,which could provide us with knowledge for advancing the elucidation of the origin of neurodegeneration.But also,polyphenols are being included in the innovative strategies of novel therapies for treating neurodegenerative diseases as well as metabolic diseases related to neurodegeneration.In this regard,flavonoid compounds are suggested as the best natural polyphenols due to their several mechanisms for acting in ameliorative effects;but increasing reports are involving other polyphenols.Even if some facts limiting bioactivity prevent them from conventional use,some natural polyphenols and derivatives hold the promise for being improved compounds,judged by their induced effects.The current results suggest polyphenols as enhancers of stem cell therapy against the targeted diseases.

Effects of a Cocktail Supplement of Ginkgo Biloba and Acai Extract on Cognitive Symptoms of Parkinson’s Disease

Parkinson’s Disease (PD) is a neurodegenerative disorder characterized by motor and non-motor symptoms, including cognitive impairment. Current treatments often involve synthetic drugs with significant side effects and potential for dependency. This study investigates the effects of a natural supplement combination of Ginkgo Biloba and Acai Extract on cognitive symptoms in a 77-year-old male with PD. The participant underwent a three-month supplementation regimen, with cognitive function assessed using the Montreal Cognitive Assessment (MoCA) test before and after the intervention. The results indicated an improvement in cognitive scores, suggesting that the combination of Ginkgo Biloba and Acai Extract may offer a promising alternative or adjunct to conventional PD treatments. This study highlights the potential of natural supplements in managing PD symptoms and calls for further research with larger sample sizes to confirm these findings. Human data was performed in accordance with the Declaration of Helsinki by the Roxbury District IRB Board (IRB Number: IRB00011767).

LC-MS法测定山楂叶中多元酚类成分的含量

【目的】利用LC-ESI-MS法测定不同山楂叶中8种主要多元酚类成分的含量。【方法】采用Lichro-spherC18(250×4.6mmI.D.,5μm)色谱柱;流动相A为乙腈,B为(0.5%甲酸溶液),梯度洗脱,0～12minA由11%～17%,12～30minA由17%～18%,30～45minA由18%～40%,45～60minA由40%～100%;流速为1ml/min,三向分流阀分流进入MSD进行检测和VWD进行监测;柱温30℃。【结果】8种多元酚类成分在不同种、不同产地及不同采收期的山楂叶中的含量存在明显的差异。【结论】本方法可用于山楂叶中多元酚类成分的测定。

Comparative metabolomics analysis of unique yellow hawthorn(Crataegus pinnatifida)and red-skinned cultivars reveals a different polyphenol biosynthesis flux and antioxidative and antidiabetic potential

Objectives:This paper aims to investigate the polyphenol metabolite variation and bioactivities of different-colored hawthorn berries(Crataegus pinnatifida).Materials and Methods:Comparative metabolomics analysis between peel and flesh of yellow hawthorn'Jinruyi'(JRY)and red-skinned ones was carried out by UPLC-MS/MS.Antioxidant activities andα-glucosidase inhibition capacity were also tested among different colored hawthorn samples.Results:A total of 453 polyphenols was characterized,among which phenolic acids and flavonoids were abundant,and were closely relevant to the antioxidant capacity of hawthorn fruits.Polyphenol profile showed accession-specific accumulation in peel or flesh of different colored hawthorn.The unique yellow hawthorn was found to have few anthocyanins but showed enhanced flux to synthesize flavones and flavonols,especially flavone C-glycosides and acylated flavonol glycosides.The specific acylation decoration included acetylation,p-coumaroylation,and malonylation.In addition,yellow hawthorn showed excellentα-glucosidase inhibitory effect,which might be associated with the high concentration of 8 polyphenols including 5 phenolic acids,2 flavone C-glycosides,and an acylated flavonol glycoside,namely,quercetin-7-O-(6ʹʹ-malonyl)glucoside.Such acylated flavonol showed the strongest correlation with the inhibition effect of hawthorn fruits onα-glucosidase,and was predicted to have the lowest binding energy with the enzyme according to molecular docking analysis,indicating its great potential as a strongα-glucosidase inhibitor and an important antidiabetic ingredient in yellow hawthorn.Conclusions:The acylated flavonol glycosides and C-glycosyl flavones might be chemotaxonomic markers differentiating varieties and bioactivities of yellow hawthorn from the traditional red-skinned ones.These findings complement the existing knowledge on the metabolite composition and nutritional properties of hawthorn fruits.

延续与转化:《S.》与《红字》的女性意识互文性解读

厄普代克"《红字》三部曲"中的最后一部小说《S.》以霍桑的代表作《红字》为前文本,从海丝特的视角出发,叙述了莎拉——现代版海丝特在当代背景下所面临的命运和困境。将《红字》与《S.》两个前后文本所体现的女性意识进行互文性解读,有助于探究美国两位伟大作家的女性意识,了解美国文化发展中女性思想的延续性与转化性。

茶多酚通过调控XB130对LPS诱导小鼠肺泡巨噬细胞炎症因子表达的影响

目的:研究茶多酚(TP)对肺炎小鼠肺泡巨噬细胞炎症因子表达的影响及潜在作用机制。方法:用脂多糖(LPS)诱导小鼠肺泡巨噬细胞MH-S 12h建立小鼠肺泡巨噬细胞炎症模型。将MH-S细胞分为对照组(LPS处理)和TP组(LPS+TP处理)、pcDNA3.1-NC组(转染pcDNA3.1-NC+LPS处理)、pcDNA3.1-XB130组(转染pcDNA3.1-XB130+LPS处理)、si-NC组(转染si-NC+LPS处理)、si-XB130组(转染si-XB130+LPS处理)、TP+si-NC组(转染si-NC+LPS+TP处理)和TP+si-XB130组(转染si-XB130组+LPS+TP处理),qRT-PCR检测XB130 mRNA表达,Western blot检测XB130表达以及白细胞介素6(IL-6)、IL-1β和肿瘤坏死因子α(TNF-α)分泌。结果:与对照组相比,TP组MH-S细胞IL-6、IL-1β、TNF-α分泌量显著降低(P<0.05),XB130 mRNA和蛋白表达显著升高(P<0.05)。与pcDNA3.1-NC组比较,pcDNA3.1-XB130组MH-S细胞IL-6、IL-1β、TNF-α分泌量显著降低,XB130蛋白表达显著升高(P<0.05)。与si-NC组比较,si-XB130组MH-S细胞IL-6、IL-1β、TNF-α分泌量显著升高,XB130蛋白表达显著降低(P<0.05)。与TP+si-NC组比较,TP+si-XB130组MH-S细胞IL-6、IL-1β、TNF-α分泌量显著升高,XB130 mRNA和蛋白表达显著降低(P<0.05)。结论:茶多酚可降低LPS诱导的小鼠肺泡巨噬细胞炎症因子表达水平,其机制与上调XB130表达有关。

A Deconstruction Perspective of The Minister's Black Veil

Deconstruction, advocated by Jacques Derrida, is a critic outlook that indicates the pitfall of the philosophical dichotomy in Western convention, revealing the unreliability of human language and conception. Hawthorne's The Minister's Black Veil,with the obscurity and uncertainty in its disclosure, offers the feasibility for a deconstruction study. The binary oppositions and its deconstruction of the "black veil" in perspective of persona, idea and existence contribute to the void perception and activities of Mr.Hooper, the protagonist as well as the author's literary practice.

Antioxidant and Phenolic Content of Nuts, Oil Seeds, Milk and Milk Products Commonly Consumed in India

Food provides not only essential nutrients required for life, but also bioactive compounds usefull to maintan good health and disease prevention. Abundant epidemiological evidences suggest that consumption of food rich in antioxi-dants (non-nutritional components) can prevent degenerative diseases. A total of 26 commonly consumed nuts, oil seeds, edible oils, milk and milk products were chosen for the study. Considering the fact that antioxidant content (AOC) and phenolic contents (PC) of these foods was not established systematically in Indian context. Therefore, we have assessed and correlated the AOC and PC, an important antioxidant constituents of plant foods. AOC was assessed by DPPH (2, 2-Diphenyl-1-picryl hydrazyl) scavenging activity and FRAP (Ferric reducing antioxidant power) methods and phenolic content (PC), using Folin-Ciocalteu reagent. Among the nuts and oil seeds arecanut had the highest phenolic and antioxidant content 10841, 4220341 mg/100g respectively. In milk, edible oils and sugars the values ranged from 336 -11674 mg/100g. Jaggery had the highest PC and AOC among the foods studied . Although AOC and PC showed wide variation among the foods, AOC was correlated significantly with PC. Indeed the ‘r’ value between PC and AOC (DPPH and FRAP) was 0.99 (p < 0.01) among the nuts and oil seeds, while in milk, milk products and sugars, the “r” values ranged from 0.93 and 0.99 (p < 0.01) respectively. The overall results indicate that the phenolic compounds may be significant contributors to the AOC of the foods studied.

Consumption of Oriental Plums Improved the Cognitive Performance and Modulated the Cerebral Neurodegeneration-Related Protein Expressions in Rats with Nicotinamide/Streptozotocin-Induced Diabetes

To examine the effect of consuming polyphenol-rich Oriental plum (Prunus salicina Lindl) on the cognitive performance and the expressions of cerebral neurodegeneration-related proteins in diabetic rats, Wistar rats were assigned into 4 groups: control (C, n = 14), nicotinamide/streptozotocin-induced DM rats (DM, n = 13), DM rats fed metformin (0.05% w/w in the diet, MT, n = 18), and DM rats fed freeze-dried oriental plum powder (2% w/w in the diet, OP, n = 16) for 2 months. The cognitive performance was evaluated by testing in a Morris water maze. The insulin resistance, serum lipid peroxidation, expressions of pathological proteins of AD, beta-amyloid (Aβ) and phosphorylated tau protein were also measured. Consumption of plums significantly improved the spatial learning ability, reduced the insulin resistance, lipid peroxidation, Aβ and phosphorylated tau protein expressions in the cerebral cortex (all P β deposition in the hippocampus of diabetic rats. In conclusion, polyphenol-rich Oriental plums ameliorated the cognitive decline and reduced the expressions of pathological proteins of AD by possibly reducing hyperglycemia, insulin resistance, and oxidative stress in diabetic rats.

Effect of Lion’s Foot (<i>Alchemilla vulgaris</i>) on Liver and Renal Functions in Rats Induced by CCl₄

The present work aims to study the influence of antioxidants activity of lion’s foot (Alchemilla vulgaris) leaves at different concentrations to give more protection against chronic liver disease. Results indicated that dried lion’s foot leaves had rich in total polyphenolic and flavonoids content (395.65 and 183.10 mg/100g, respectively). These results were reflected to the antioxidant activity (DPPH);it’s noticed that the antioxidant activity of dried lion’s foot leaves was high (131.74%). The major polyphenolic components were benzoic acid (1084.63 ppm) followed by ellagic acid, catechol, and catechin (614.16, 580.54, and 566.53 ppm, respectively) then salicylic acid and protocatechuic acid (479.71 and 444.43 ppm, respectively). On the same trend, flavonoids fractions indicated the highest content in luteo-6-arabinase 8-glucose, apig. 6-rhamnase 8-glucose, acatein, narengin and luteolin (40.01;15.04;8.07;6.64 and 6.42 ppm, respectively). Fifty-six male albino rats were used in biological experiments. Rats fed on basal diet for two weeks before the performance of the experiment. At the beginning, rats divided into eight main group were fed on diets for 45 days as follows: Negative control group (first group) was fed on basal diet. Forty nine rats were fed on basal diet and induced by CCl4, in paraffin oil (50% v/v, 2 ml/Kg) twice weeks subcutaneous injection to induce chronic damage in the liver, then divided into 7 groups numbered from group 2 to group 8. Positive control group rats fed on basal diet till final experiment (second group). Group 3 and 4 rats treated with 50 and 100 ppm ethanolic leaves extracts, respectively. Also, group 5 and 6 treated with 50 and 100 ppm aqueous leaves extracts, respectively. All extracts were fed on orally every day. While, rats in group 7 treated with 1% and 2% dried lion’s foot leaves. At the end of the experimental period, serums were collected to determine liver and renal functions. The liver was removed surgically for histopathological observation. The results revealed that CCl4 intoxication impaired liver function. Serum AST, ALT, ALP and total bilirubin levels were elevated by CCl4 administration, while significant decreasing was noticed in serum albumin in CCl4 group. Histopathologically, CCl4 caused congestion of central vain, fatty change of hepatocytes, and focal inflammatory cells in filtration. Treatment with lion’s foot with different forms and concentration attenuated these adverse effects and markedly ameliorated histopathological and biochemical alterations caused by CCl4 especially with 2% powder and 100 ppm ethanol extract administration. Therefore, the results of this study concluded that lion’s foot can be proposed to protect hepatotoxicity induced by CCl4 in rats. The results also revealed that the hepatoprotection effect of lion’s foot may be attributed to its antioxidant contents and free radical scavenger effect.

Nutritional Value of Italian Pistachios from Bronte ( <i>Pistacia vera</i>, L.), Their Nutrients, Bioactive Compounds and Antioxidant Activity

This study gives an overview on the nutritional value, bioactive compounds and antioxidant activity (ABTS and FRAP) of Bronte’s pistachio (Pistacia vera, L., cv. Bianca) from Sicily (Italy). Bronte’s pistachios are rich in fat, protein, dietary fiber, trace elements (Fe, Zn, Cu, Mn) and minerals (Ca, P, K, Mg, Na). Lipids mainly consist of MUFA (33.8 g/100g), primarily oleic acid (32.4 g/ 100g);PUFA is mostly represented by linoleic acid (7.49 g/100g). Bronte’s pistachios are also a valuable source of bioactive compounds such as total polyphenols (501.5 mg/100g), lutein (1.26 mg/100g), β-carotene (0.18 mg/100g), γ-tocopherol (19.2 mg/100g) and phytosterols (134.4 mg/100g). Among phytosterols, the main is β-sitosterol (86% of total content), followed by Δ5-avenasterol (6.3%). Phytic acid content is 1763 mg/100g;Ins(1,2,4,5,6)P5 and Ins(1,2,3,4,5,)P5 have been detected too (31 mg/100g and 10 mg/100g, respectively). The antioxidant activity was determined both in hydrophilic and lipophilic fraction of pistachios, showing >80% of the total antioxidant activity in the hydrophilic, predominately due to phenolic compounds.

不同产地商品山楂干的品质差异分析

本研究以10种不同产地的商品山楂干[包括7种普通山楂(商品名“北山楂”)、2种台湾林檎(商品名“广山楂”)和1种野山楂(商品名“南山楂”)]为研究对象,测定其主要营养成分和生化指标,并在此基础上对山楂进行品质差异分析和主成分分析。结果表明:在3类商品山楂干中,北山楂的可滴定酸含量最高,广山楂的糖酸比最高,而可溶性膳食纤维、α-生育酚和β-胡萝卜素含量排序均为北山楂>南山楂>广山楂。主成分分析结果表明,湖北黄冈的‘野山楂’和天津蓟州的‘铁山楂’在主要活性成分(总酚、总黄酮、总原花青素、总膳食纤维)含量方面整体表现较优;山东临沂的‘大金星’和河南新乡的‘豫北红’等北山楂在可滴定酸、可溶性膳食纤维和可溶性糖含量方面相较于南山楂和广山楂综合表现更优。本研究系统梳理了我国不同产区主要种类商品山楂干的品质差异,可为我国山楂资源的多元化开发利用提供数据支撑。

响应面法优化山楂多酚微粒制备工艺

本研究依据聚电解质自组装原理将山楂多酚制备为山楂多酚微粒(Hawthorn polyphenol microparticles,HPM)。通过Plackett-Burman联用Box-Behnken设计,以山楂多酚的包封率和载药量为响应值对HPM的制备工艺因素进行研究。采用Plackett-Burman设计筛选对实验结果影响较为显著的因素(制备时间、硫酸浓度和包封材料比例)进行Box-Behnken响应曲面优化试验。利用SEM和TEM观测HPM的形貌,并利用模拟胃肠液研究HPM的胃肠道释放动力学。结果表明:制备时间、硫酸浓度和包封材料比例对HPM的制备具有明显影响,制备时间为3 h,硫酸浓度为0.0254%,壳聚糖与海藻酸钠比例为4:1时HPM的包封率为96.27%±1.37%。对HPM的形貌研究显示,其为不规则的圆形颗粒且分散性良好。HPM在模拟肠环境中的释放速度大于模拟胃环境。其在胃液和肠液中的释放分别属于零级和一级动力学。综上所述,本研究制备出一种包封率、载药量较高且分散性良好的HPM,并且制备的HPM可以降低胃肠道对山楂多酚的降解,为山楂多酚的食品添加提供了新的思路。

山楂不同提取物成分分析及其生物活性研究

山楂富含果糖、葡萄糖、脂肪类物质、蛋白质、粗纤维和胡萝卜素等,具有抗炎、降血糖和降血压等生物活性功能,被广泛应用于食品添加剂、调味品和保健品等方面。山楂中活性成分提取方式多样,不同提取方式获得的化合物成分和含量存在差异。目前,溶剂提取法是最常见的提取方式,该方法技术要求低、操作简单,但是不同溶剂提取获得的化合物成分和含量存在明显差异。该试验采用高效液相色谱法测定6种不同溶剂(甲醇、乙醇、丙酮、乙酸乙酯、氯仿和石油醚)的山楂提取物中多酚、黄酮等活性成分含量,结果表明,山楂甲醇提取物中多酚和黄酮含量最高,山楂石油醚提取物中多酚和黄酮含量最低;6种不同溶剂的山楂提取物中鉴定出10种活性成分,分别为儿茶素、绿原酸、原花青素B2、表儿茶素、槲皮素、山楂酸、金丝桃苷、异槲皮苷、咖啡酸、木犀草素葡萄糖甙。通过对不同溶剂提取的山楂化合物的抗氧化性和还原性进行分析,结果表明,随着山楂提取物浓度的增加,抗氧化性和还原性也增加,山楂甲醇提取物的抗氧化性和还原性相较于其他溶剂的山楂提取物更强。

深共熔溶剂提取酒糟多酚类物质及其体外抗氧化、降血糖活性研究

为了充分利用酒糟中多酚类活性成分资源,首先从10种深共熔溶剂中筛选最适宜酒糟多酚类物质提取的体系组合,采用响应曲面法优化深共熔溶剂提取酒糟多酚的工艺,运用高效液相色谱分析酒糟多酚类物质,并评估酒糟多酚体外抗氧化和降血糖活性。结果表明,酒糟多酚最佳的深共熔溶剂体系为氯化胆碱-果糖,最佳提取工艺为深共熔溶剂含水率41%,提取时间31 min,液料比31∶1(mL∶g),提取温度40℃,在此条件下酒糟多酚含量为(6.28±0.18)mg GAE/g。并且在检测的酚类物质中,对香豆酸含量(466.7±0.20)μg/g最高,占7种酚类物质的30%左右。酒糟多酚清除羟自由基、DPPH自由基和ABTS阳离子自由基的IC_(50)值分别为0.886、0.291、0.302 mg/mL,结合抑制α-淀粉酶和α-葡萄糖苷酶活力的IC_(50)值分别为0.938和2.329 mg/mL,表明酒糟多酚具有较强抗氧化和降血糖能力。深共熔溶剂提取酒糟多酚切实可行,并且酒糟多酚提取物具有较强的体外抗氧化和降血糖活性。

热风干燥温度对山楂粉品质的影响

为了研究不同热风干燥温度对山楂干燥产品品质的影响。以鲜山楂为原料,设置不同的温度梯度,以干燥时间、干燥速率、休止角、堆积密度、分散性、持水性、总酚含量、总黄酮含量及抗氧化活性为指标,考察不同温度下得到的山楂粉品质。结果表明:在干燥温度为70、80、90、100、110℃时,山楂粉到达干燥终点的时间分别为200、160、140、120、80 min,干燥温度越高,所需时间越短。随干燥温度的升高,休止角逐渐增大,堆积密度先降低后升高,持水性逐渐降低。80℃下得到的山楂粉总酚含量最高,为5.415 mg/g,且其对1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基和2,2-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基有较高的清除率,总还原能力与其他组相比较高。综合结果可知,热风干燥温度为80℃得到的山楂粉中总酚及总酮的保存率较高,且品质最好。

苹果山楂番石榴提取物体外协同降糖降脂作用研究

以苹果、山楂、番石榴为原料,利用超声辅助法提取苹果中的多酚、山楂中的黄酮、番石榴中的多糖作为α-淀粉酶、α-葡萄糖苷酶和胰脂肪酶的抑制剂,以抑制率为指标,采用单因素试验和正交试验对3种物质协同降糖降脂作用进行研究。结果表明,3种提取物复配质量浓度为番石榴多糖1.25 mg/mL,苹果多酚1.5 mg/mL,山楂黄酮1.5 mg/mL时,体外协同抑制α-淀粉酶和α-葡萄糖苷酶活效果较强,其协同抑制α-淀粉酶活性和α-葡萄糖苷酶的抑制率可达到88.5%和90.2%;体外抑制胰脂肪酶效果最强复配质量浓度为山楂黄酮3 mg/mL、苹果多酚1 mg/mL、番石榴多糖9 mg/mL,此条件下,抑制率为70.01%,研究结果对降糖、降脂食品的研发有一定的指导意义。

山楂叶中主要多元酚类成分的HPLC法比较分析(英文)

目的:比较不同种、不同产地及不同采收期山楂叶中8种主要多元酚类成分(牡荆素、牡荆素2″O 鼠李糖苷、芦丁、绿原酸、槲皮素、金丝桃苷、4 O 葡萄糖牡荆素和4 O 鼠李糖芦丁)的含量。方法:用高效液相色谱(HPLC)法。色谱柱为LichrospherC18(250×4.6mmI.D.,5μm);流动相A为乙腈-四氢呋喃(95∶5)、B为0.5%磷酸水溶液,梯度洗脱,0 ～12minA由12%～17%,12～25minA由17%～18%,25～35minA由18%～40%,35～40minA由40%～100%,40～45 minA为100%,流速1ml/min;柱温30℃;进样量10μl;检测波长360nm。结果:野山楂叶中这8种多元酚类成分的总含量最高(3.70%～6.20%),其次是山里红叶(1.12%～2.28%),再次是单子山楂叶(0.98%),云南山楂叶中这8种成分的含量最低(0.70%～0.87%)。对同种的山楂叶来说,其含量随着产地及采收期的不同有着较大差异,含量最高的野山楂叶采自江西于都县,山里红叶中含量最高的是采自山东日照市;对不同时期采收的山里红叶分析表明,嫩叶中尤其是开花期(五月)采收的叶中这8种成分的含量最高,然后慢慢降低到一个相对稳定的水平。结论:不同种、不同产地及不同采收期的山楂叶中这8种主要多元酚类成分的含量存在明显的差异。

茶多酚复合溶液对山楂涂膜保鲜效果的研究

为了延长山楂的货架寿命,减少其在储运过程中水分和营养物质损失。以茶多酚、胶原蛋白、明胶、甘油为主要原料制备复合涂膜保鲜液,对山楂进行涂膜处理。在(20±2)℃、60%的相对湿度条件下贮藏,测定山楂的硬度、维生素C含量、感官品质和失重率等指标变化情况,并评价复合涂膜保鲜液的保鲜效果。结果表明,贮藏20天后,经涂膜处理的山楂失重率低于10%,感官评分可达3分以上,硬度高于2.5×10~5Pa/cm^2,维生素C含量高于90mg/100g,均优于未涂膜组。与对照组相比,其货架期可延长12天。优化的涂膜保鲜液配比为:4.0%胶原蛋白、3.0%明胶、0.2%茶多酚、1.0%甘油。