Lentinan is a clinically approved immune modulator and its anticancer and immunomodulatory bioactivity is found to be dependent on its triple helical conformation. Therefore, the development of rapid and convenient me...Lentinan is a clinically approved immune modulator and its anticancer and immunomodulatory bioactivity is found to be dependent on its triple helical conformation. Therefore, the development of rapid and convenient method for determination of bioactive lentinan with triple helical conformation holds great promise for the quality control of lentinan healthy products. In this work, an aniline blue fluorescent method was optimized and established to accurately and rapidly detect bioactive lentinan. In the presence of lentinan, the fluorescence intensity of aniline blue with 404 nm excitation and 492 nm emission dramatically enhanced within 15 min in pH 10 glycine-NaOH buffer solution, which allowed the analysis of lentinan in a very simple and fast manner. The method allowed for the sensitive determination of lentinan in the range of 1 to 60 μg/mL with a detection limit of 0.25 μg/mL. Notably, the protocol exhibited excellent selectivity for the determination of triple helical lentinan over other saccharides. The method was successfully applied to the detection of bioactive lentinan in health tonic solution, which demonstrated the method had great potential for quality control of lentinan contained products.展开更多
Objective: To explore the nutritional and health promoting effects of nutrient mixture (traditional Chinese tonics ＋ nutrient fortification). Methods: (1) One hundred and three primigravid women were divided randoml...Objective: To explore the nutritional and health promoting effects of nutrient mixture (traditional Chinese tonics ＋ nutrient fortification). Methods: (1) One hundred and three primigravid women were divided randomly into the test group and the control group treated with nutrient mixture and vitamins respectively and the effects of treatment were observed. (2) Effects of nutrient mixture were also observed in pregnant rats. Results: (1) Clinical study showed the serum levels of iron, zinc, hemoglobin of the test group were allraised after treatment, and these parameters and the concentration of iron in umbilical cord blood as well as the weight of the new-born baby of the test group were higher than those of the control group (P ＜0. 05 or P＜0. 01). (2) Results of the experimental study showed that the food utilization rate, hemoglobin level, fetal growth and developing parameters of the test group were all higher than those of the control group significantly (P ＜0. 05 or P＜ 0. 01 ). Conclusion: The nutrient mixture has a good nutritional and health promoting effect on pregnancy.展开更多
文摘Lentinan is a clinically approved immune modulator and its anticancer and immunomodulatory bioactivity is found to be dependent on its triple helical conformation. Therefore, the development of rapid and convenient method for determination of bioactive lentinan with triple helical conformation holds great promise for the quality control of lentinan healthy products. In this work, an aniline blue fluorescent method was optimized and established to accurately and rapidly detect bioactive lentinan. In the presence of lentinan, the fluorescence intensity of aniline blue with 404 nm excitation and 492 nm emission dramatically enhanced within 15 min in pH 10 glycine-NaOH buffer solution, which allowed the analysis of lentinan in a very simple and fast manner. The method allowed for the sensitive determination of lentinan in the range of 1 to 60 μg/mL with a detection limit of 0.25 μg/mL. Notably, the protocol exhibited excellent selectivity for the determination of triple helical lentinan over other saccharides. The method was successfully applied to the detection of bioactive lentinan in health tonic solution, which demonstrated the method had great potential for quality control of lentinan contained products.
文摘Objective: To explore the nutritional and health promoting effects of nutrient mixture (traditional Chinese tonics ＋ nutrient fortification). Methods: (1) One hundred and three primigravid women were divided randomly into the test group and the control group treated with nutrient mixture and vitamins respectively and the effects of treatment were observed. (2) Effects of nutrient mixture were also observed in pregnant rats. Results: (1) Clinical study showed the serum levels of iron, zinc, hemoglobin of the test group were allraised after treatment, and these parameters and the concentration of iron in umbilical cord blood as well as the weight of the new-born baby of the test group were higher than those of the control group (P ＜0. 05 or P＜0. 01). (2) Results of the experimental study showed that the food utilization rate, hemoglobin level, fetal growth and developing parameters of the test group were all higher than those of the control group significantly (P ＜0. 05 or P＜ 0. 01 ). Conclusion: The nutrient mixture has a good nutritional and health promoting effect on pregnancy.
