Objective:This study aimed to elucidate the possible mechanism of Gest-Aid Plus Oral Liquid(GAP)on healthcare function.Method:Ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-bas...Objective:This study aimed to elucidate the possible mechanism of Gest-Aid Plus Oral Liquid(GAP)on healthcare function.Method:Ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-based metabolomics and 16S rDNA sequencing of gut microbiota were performed on serum and fecal samples of GAP and control rats.Additionally,short-chain fatty acids(SCFAs)and inflammatory cytokines in fecal samples were determined through gas chromatography-mass spectrometry and enzyme-linked immunosorbent assay kits.Result:Metabolomics discovered 41 metabolites,which mainly involved amino acid metabolism,lipid metabolism,coenzyme factors,and vitamin metabolism.Administration of GAP increased abundance of Prevotella_9,Alloprevotella,Blautia,Phascolarctobacterium,Parabacteroides,and Fusicatenibacter,and six SCFAs were increased in the GAP group.Measurement of inflammatory cytokines showed that GAP had an anti-inflammatory effect in rats.Conclusions:Administration of GAP greatly affects the aspartate metabolism and microecology of rats,enhances intestinal motility and gut barrier integrity and anti-inflammation.These findings not only have possible implications for further application of GAP,but also provide a link between the gut microbiome,SCFAs,inflammation and serum metabolites in rats.展开更多
文摘Objective:This study aimed to elucidate the possible mechanism of Gest-Aid Plus Oral Liquid(GAP)on healthcare function.Method:Ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-based metabolomics and 16S rDNA sequencing of gut microbiota were performed on serum and fecal samples of GAP and control rats.Additionally,short-chain fatty acids(SCFAs)and inflammatory cytokines in fecal samples were determined through gas chromatography-mass spectrometry and enzyme-linked immunosorbent assay kits.Result:Metabolomics discovered 41 metabolites,which mainly involved amino acid metabolism,lipid metabolism,coenzyme factors,and vitamin metabolism.Administration of GAP increased abundance of Prevotella_9,Alloprevotella,Blautia,Phascolarctobacterium,Parabacteroides,and Fusicatenibacter,and six SCFAs were increased in the GAP group.Measurement of inflammatory cytokines showed that GAP had an anti-inflammatory effect in rats.Conclusions:Administration of GAP greatly affects the aspartate metabolism and microecology of rats,enhances intestinal motility and gut barrier integrity and anti-inflammation.These findings not only have possible implications for further application of GAP,but also provide a link between the gut microbiome,SCFAs,inflammation and serum metabolites in rats.
