This paper focuses on the process for pillow shape plate by line heating technique, which is widely applied in the production of ship hull. Based on the analysis of primary parameters and experimental data in line hea...This paper focuses on the process for pillow shape plate by line heating technique, which is widely applied in the production of ship hull. Based on the analysis of primary parameters and experimental data in line heating process, the amount of local contraction generated by line heating has been illustrated. Then, combining with the computational result of local deformation determined by shell plate development, an optimization method for line heating parameters has been studied. This prediction system may provide rational arrangements of heating lines and technical parameters of process. By integrating the prediction system into the line heating robot for pillow shape plate, the automatic process of line heating for pillow shape plate can be achieved.展开更多
Based on experimental data of line heating, the methods of vector mapping, plane projection, and coordinate converting are presented to establish the spectra for line heating distortion discipline which shows the rela...Based on experimental data of line heating, the methods of vector mapping, plane projection, and coordinate converting are presented to establish the spectra for line heating distortion discipline which shows the relationship between process parameters and distortion parameters of line heating. Back-propagation network (BP-net) is used to modify the spectra.Mathematical models for optimizing line heating techniques parameters, which include two-objective functions,are constructed. To convert the multi-objective optimization into a single-objective one, the method of changing weight coefficient is used, and then the individual fitness function is built up. Taking the number of heating lines, distance between the heating lines’ border (line space), and shrink quantity of lines as three restrictive conditions,a hierarchy genetic algorithm (HGA) code is established by making use of information provided by the spectra, in which inner coding and outer coding adopt different heredity arithmetic operators in inherent operating. The numerical example shows that the spectra for line heating distortion discipline presented here can provide accurate information required by techniques parameter prediction of line heating process and the technique parameter optimization method based on HGA provided here can obtain good results for hull plate.展开更多
Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cel...Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. In this paper, we report the development of ht cell lines from other insect species and examination of their growth characteristics and virus susceptibility. Adaptation of cell lines sf-9, BTI-TN-5B1-4 (High5) and BTI-TN-MG1 (MG1) to 33℃ and 35℃ was carried out by shifting the culture temperature between 28℃ and higher temperatures by a gradual stepwise increase in temperature. The process of adaption to a higher culture temperature was accomplished over a period of 2 months. The cell lines with the temperature adaption were designated as sf9-ht33, sf9-ht35, High5-ht33, High5-ht35, MG1-ht33, MG1-ht35. These cell lines have been subcultured over 70 passages. Adaption to high temperatures was confirmed by a constant population doubling time with individual cell lines. The population doubling time of heat adapted cell lines were 1-4 h less than these of parental cell lines. Cell shapes did not show obvious change, however, the cell size of sf9-ht cells was enlarged and those of High5 and MG1 ht cells were reduced after heat adaption. When the cell lines were infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) at 28℃, 33℃, 35℃ and 37℃, production of budded virus and occlusion bodies in each cell line was optimum at its own adapted temperature.展开更多
In the present study, a japonica rice ( Oryza sativa L. ) variety Nipponbare, an indica variety 9311 and a set of chromosome segment substitution lines (CSSLs) which were generated using Nipponbare as the recipien...In the present study, a japonica rice ( Oryza sativa L. ) variety Nipponbare, an indica variety 9311 and a set of chromosome segment substitution lines (CSSLs) which were generated using Nipponbare as the recipient parent and 9311 as the donor parent were used as the experimental materials. The CSSLs were grown in 2012 (normal temperature condition) and 2013 (high temperature condition) in Yangzhou, Jiangsu, and were used to map the quantitative trait loci (QTLs) for heat tolerance, based on the heat tolerance index [ (The seed setting rate under normal temperature condition -The seed setting rate under high temper- ature condition) / The seed setting rate under normal temperature condition]. As a result, three QTLs related to heat tolerance in rice were mapped on chromo- somes 2, 4 and 12, respectively. They had LOD (logarithm of rntds) scores of 2.56, 4.02 and 2.79, and contributian rates of 4.95%, 7.99% and 5.44%. Among them, qHT12.1 showed positive effect, while qHT2.1 and qHT4. t showed negative effect on heat tolerance. The results lay a foundation for the fine mapping and cloning of the QTLs and genes related to heat tolerance, and for the breeding of heat-tolerant rice varieties.展开更多
High-temperature heating surface such as superheater and reheater of large-sized utility boiler all experiences a relatively severe working conditions. The failure of boiler tubes will directly impact the safe and eco...High-temperature heating surface such as superheater and reheater of large-sized utility boiler all experiences a relatively severe working conditions. The failure of boiler tubes will directly impact the safe and economic operation of boiler. An on-line life monitoring model of high-temperature heating surface was set up according to the well-known L-M formula of the creep damages. The tube wall metal temperature and working stress was measured by on-line monitoring, and with this model, the real-time calculation of the life expenditure of the heating surface tube bundles were realized. Based on the technique the on-line life monitoring and management system of high-temperature heating surface was developed for a 300 MW utility boiler. An effective device was thus suggested for the implementation of the safe operation and the condition-based maintenance of utility boilers.展开更多
文摘This paper focuses on the process for pillow shape plate by line heating technique, which is widely applied in the production of ship hull. Based on the analysis of primary parameters and experimental data in line heating process, the amount of local contraction generated by line heating has been illustrated. Then, combining with the computational result of local deformation determined by shell plate development, an optimization method for line heating parameters has been studied. This prediction system may provide rational arrangements of heating lines and technical parameters of process. By integrating the prediction system into the line heating robot for pillow shape plate, the automatic process of line heating for pillow shape plate can be achieved.
文摘Based on experimental data of line heating, the methods of vector mapping, plane projection, and coordinate converting are presented to establish the spectra for line heating distortion discipline which shows the relationship between process parameters and distortion parameters of line heating. Back-propagation network (BP-net) is used to modify the spectra.Mathematical models for optimizing line heating techniques parameters, which include two-objective functions,are constructed. To convert the multi-objective optimization into a single-objective one, the method of changing weight coefficient is used, and then the individual fitness function is built up. Taking the number of heating lines, distance between the heating lines’ border (line space), and shrink quantity of lines as three restrictive conditions,a hierarchy genetic algorithm (HGA) code is established by making use of information provided by the spectra, in which inner coding and outer coding adopt different heredity arithmetic operators in inherent operating. The numerical example shows that the spectra for line heating distortion discipline presented here can provide accurate information required by techniques parameter prediction of line heating process and the technique parameter optimization method based on HGA provided here can obtain good results for hull plate.
基金Chinese National Basic Research Program(973)2009CB118900Chinese National ScienceFoundation Project(30771451)Boyce Thompson Institutefor Plant Research Project(BTI-QAU1-23-2007)
文摘Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. In this paper, we report the development of ht cell lines from other insect species and examination of their growth characteristics and virus susceptibility. Adaptation of cell lines sf-9, BTI-TN-5B1-4 (High5) and BTI-TN-MG1 (MG1) to 33℃ and 35℃ was carried out by shifting the culture temperature between 28℃ and higher temperatures by a gradual stepwise increase in temperature. The process of adaption to a higher culture temperature was accomplished over a period of 2 months. The cell lines with the temperature adaption were designated as sf9-ht33, sf9-ht35, High5-ht33, High5-ht35, MG1-ht33, MG1-ht35. These cell lines have been subcultured over 70 passages. Adaption to high temperatures was confirmed by a constant population doubling time with individual cell lines. The population doubling time of heat adapted cell lines were 1-4 h less than these of parental cell lines. Cell shapes did not show obvious change, however, the cell size of sf9-ht cells was enlarged and those of High5 and MG1 ht cells were reduced after heat adaption. When the cell lines were infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) at 28℃, 33℃, 35℃ and 37℃, production of budded virus and occlusion bodies in each cell line was optimum at its own adapted temperature.
基金supported by the National Natural Science Foundation of China(Grant No.30671230)the Natural Science Foundation of Jiangxi Province,China(Grant No.2007GZN0253)
基金Supported by the National Natural Science Foundation of China(31561143008,31401354)Jiangsu Provincial Natural Science Foundation(BK20140484)Doctoral Fund of Ministry of Education of China(20133250120001)
文摘In the present study, a japonica rice ( Oryza sativa L. ) variety Nipponbare, an indica variety 9311 and a set of chromosome segment substitution lines (CSSLs) which were generated using Nipponbare as the recipient parent and 9311 as the donor parent were used as the experimental materials. The CSSLs were grown in 2012 (normal temperature condition) and 2013 (high temperature condition) in Yangzhou, Jiangsu, and were used to map the quantitative trait loci (QTLs) for heat tolerance, based on the heat tolerance index [ (The seed setting rate under normal temperature condition -The seed setting rate under high temper- ature condition) / The seed setting rate under normal temperature condition]. As a result, three QTLs related to heat tolerance in rice were mapped on chromo- somes 2, 4 and 12, respectively. They had LOD (logarithm of rntds) scores of 2.56, 4.02 and 2.79, and contributian rates of 4.95%, 7.99% and 5.44%. Among them, qHT12.1 showed positive effect, while qHT2.1 and qHT4. t showed negative effect on heat tolerance. The results lay a foundation for the fine mapping and cloning of the QTLs and genes related to heat tolerance, and for the breeding of heat-tolerant rice varieties.
文摘High-temperature heating surface such as superheater and reheater of large-sized utility boiler all experiences a relatively severe working conditions. The failure of boiler tubes will directly impact the safe and economic operation of boiler. An on-line life monitoring model of high-temperature heating surface was set up according to the well-known L-M formula of the creep damages. The tube wall metal temperature and working stress was measured by on-line monitoring, and with this model, the real-time calculation of the life expenditure of the heating surface tube bundles were realized. Based on the technique the on-line life monitoring and management system of high-temperature heating surface was developed for a 300 MW utility boiler. An effective device was thus suggested for the implementation of the safe operation and the condition-based maintenance of utility boilers.