The first intron of human hsp90β gene is not only essential in maintaining high constitutive expression but also critical for heat shock inducibility of the hsp90β gene. Typical HSEs in ...The first intron of human hsp90β gene is not only essential in maintaining high constitutive expression but also critical for heat shock inducibility of the hsp90β gene. Typical HSEs in the first intron play a vital role in the heat induced expression of human hsp90β gene. Slot blot analysis shows that hsp90β gene mRNA transcripts initiated from the 3’ of the first intron dominates over that of the first exon. The intronic HSEs of the hsp90β gene show much higher binding affinity toward the recombinant heat shock factor HSF1 than that of the recombinant heat shock factor HSF2.展开更多
[Objectives]To investigate the effects of different temperature and starvation stress on the expression of AaHsp90 and reveal the molecular mechanism of adaptation to environment in Antheraea assama.[Methods]Taking th...[Objectives]To investigate the effects of different temperature and starvation stress on the expression of AaHsp90 and reveal the molecular mechanism of adaptation to environment in Antheraea assama.[Methods]Taking the normal feeding group at 26℃as the control,the expression change of AaHsp90 was detected by real-time PCR in midgut,fat body and hemlymph after high temperature stress at 38℃,low temperature stress at 4℃and starvation stress separately for different time on the third day of the fifth larvae.[Results]The expression of AaHsp90 in midgut,fat body and hemlymph of Antheraea assama were increased obviously at first and then decreased sharply with the prolongation of treatment time at 38℃.There has a certain inhibitory effect on the expression of AaHsp90 in midgut,fat body and hemolymph after treatment with 4℃for different time.After treatment with starvation,the AaHsp90 expression were increased at 12 and 18 h and decreased sharply at 24 h in midgut,fat body and hemolymph of A.assama.[Conclusions]Comprehensive analysis showed that high temperature and starvation stress can induce the expression of AaHsp90,while low temperature stress mainly suppressed its expression.It was suggested that the AaHsp90 protein may play an important role in the process of adaptation to high temperature and starvation stress in A.assama.展开更多
三阴性乳腺癌(triple negative breast cancer,TNBC)恶性程度高,预后差,其p53基因的突变频率达80%以上。日蟾蜍它灵(gamabufotalin,CS-6)是日本蟾蜍的皮肤分泌物,有研究表明CS-6具有抗癌作用,但其对TNBC的作用和机制尚无报道。本文采用...三阴性乳腺癌(triple negative breast cancer,TNBC)恶性程度高,预后差,其p53基因的突变频率达80%以上。日蟾蜍它灵(gamabufotalin,CS-6)是日本蟾蜍的皮肤分泌物,有研究表明CS-6具有抗癌作用,但其对TNBC的作用和机制尚无报道。本文采用MTT法和细胞克隆形成实验检测了不同浓度CS-6对TNBC细胞系MDA-MB-468和MDA-MB-231细胞增殖的影响;采用Western-blot检测了CS-6在外源和内源水平对p53和热休克蛋白90(heat shock protein 90,HSP90)表达水平的影响;采用联合指数及等效线分析法考察了CS-6和HSP90的抑制剂坦螺旋霉素(tanespimycin,17-AAG)对MDA-MB-468和MDA-MB-231细胞增殖的协同作用。结果显示,CS-6明显抑制MDA-MB-468和MDA-MB-231细胞的增殖,其抑制增殖机制有可能与降解HSP90/突变p53有关;而且,CS-6和HSP90抑制剂17-AAG联合用药对抑制MDA-MB-468和MDA-MB-231细胞增殖具有协同作用。展开更多
为研究猪精液热休克蛋白90(heat-shock protein 90,Hsp90)的定位以及表达特性,通过RT-PCR、蛋白质印迹、免疫组化、免疫荧光方法,检测和分析Hsp90在猪繁殖组织(睾丸和卵巢)、生殖细胞(精子细胞和卵母细胞)中的定位以及表达.RT-PCR结果显...为研究猪精液热休克蛋白90(heat-shock protein 90,Hsp90)的定位以及表达特性,通过RT-PCR、蛋白质印迹、免疫组化、免疫荧光方法,检测和分析Hsp90在猪繁殖组织(睾丸和卵巢)、生殖细胞(精子细胞和卵母细胞)中的定位以及表达.RT-PCR结果显示,Hsp90在睾丸和附睾尾表达,在卵巢中微量表达;蛋白质印迹结果显示,Hsp90蛋白在睾丸、卵巢、输卵管和尿道球腺中高表达;免疫组化结果显示,Hsp90蛋白在睾丸的间质细胞、次级精母细胞和精子细胞中表达,也在卵巢的卵母细胞、卵丘细胞和颗粒细胞中表达;免疫荧光结果显示,Hsp90蛋白定位在精子的颈部、尾中部、卵丘细胞的细胞质和卵母细胞中.综上所述,Hsp90在猪主要的繁殖组织和生殖细胞中高表达,其表达水平可能与精子质量和胚胎发育有关.展开更多
Objective:Hepatocellular carcinoma(HCC),the main type of liver cancer,has a high morbidity and mortality,and a poor prognosis.RNA helicase DDX5,which acts as a transcriptional co-regulator,is overexpressed in most mal...Objective:Hepatocellular carcinoma(HCC),the main type of liver cancer,has a high morbidity and mortality,and a poor prognosis.RNA helicase DDX5,which acts as a transcriptional co-regulator,is overexpressed in most malignant tumors and promotes cancer cell growth.Heat shock protein 90(HSP90)is an important molecular chaperone in the conformational maturation and stabilization of numerous proteins involved in cell growth or survival.Methods:DDX5 m RNA and protein expression in surgically resected HCC tissues from 24 Asian patients were detected by quantitative real-time PCR and Western blot,respectively.The interaction of DDX5-HSP90 was determined by molecular docking,immunoprecipitation,and laser scanning confocal microscopy.The autophagy signal was detected by Western blot.The cell functions and signaling pathways of DDX5 were determined in 2 HCC cell lines.Two different murine HCC xenograft models were used to determine the function of DDX5 and the therapeutic effect of an HSP90 inhibitor.Results:HSP90 interacted directly with DDX5 and inhibited DDX5 protein degradation in the AMPK/ULK1-regulated autophagy pathway.The subsequent accumulation of DDX5 protein induced the malignant phenotype of HCC by activating theβ-catenin signaling pathway.The silencing of DDX5 or treatment with HSP90 inhibitor both blocked in vivo tumor growth in a murine HCC xenograft model.High levels of HSP90 and DDX5 protein were associated with poor prognoses.Conclusions:HSP90 interacted with DDX5 protein and subsequently protected DDX5 protein from AMPK/ULK1-regulated autophagic degradation.DDX5 and HSP90 are therefore potential therapeutic targets for HCC.展开更多
<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels ...<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. Results: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1±3.2 and 243.0±21.6, respectively, while those after thawing were 23.2±2.5 and 105.7±28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. Conclusion: HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.展开更多
Previous studies have confirmed that heat shock protein 90 overexpression can lead to dopami- nergic neuronal death. This study was designed to further investigate what effects are produced by heat shock protein 90 af...Previous studies have confirmed that heat shock protein 90 overexpression can lead to dopami- nergic neuronal death. This study was designed to further investigate what effects are produced by heat shock protein 90 after endurance exercise training. Immunohistochemistry results showed that exercise training significantly inhibited heat shock protein 90 overexpression in the soleus and gastrocnemius in Parkinson＇s disease rats, which is a potential therapeutic target for ameliorating skeletal muscle abnormalities in Parkinso^s disease.展开更多
文摘The first intron of human hsp90β gene is not only essential in maintaining high constitutive expression but also critical for heat shock inducibility of the hsp90β gene. Typical HSEs in the first intron play a vital role in the heat induced expression of human hsp90β gene. Slot blot analysis shows that hsp90β gene mRNA transcripts initiated from the 3’ of the first intron dominates over that of the first exon. The intronic HSEs of the hsp90β gene show much higher binding affinity toward the recombinant heat shock factor HSF1 than that of the recombinant heat shock factor HSF2.
基金Supported by Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities Association (2021BA070001-070)Yunnan Fundamental Research Projects (202201AT070226).
文摘[Objectives]To investigate the effects of different temperature and starvation stress on the expression of AaHsp90 and reveal the molecular mechanism of adaptation to environment in Antheraea assama.[Methods]Taking the normal feeding group at 26℃as the control,the expression change of AaHsp90 was detected by real-time PCR in midgut,fat body and hemlymph after high temperature stress at 38℃,low temperature stress at 4℃and starvation stress separately for different time on the third day of the fifth larvae.[Results]The expression of AaHsp90 in midgut,fat body and hemlymph of Antheraea assama were increased obviously at first and then decreased sharply with the prolongation of treatment time at 38℃.There has a certain inhibitory effect on the expression of AaHsp90 in midgut,fat body and hemolymph after treatment with 4℃for different time.After treatment with starvation,the AaHsp90 expression were increased at 12 and 18 h and decreased sharply at 24 h in midgut,fat body and hemolymph of A.assama.[Conclusions]Comprehensive analysis showed that high temperature and starvation stress can induce the expression of AaHsp90,while low temperature stress mainly suppressed its expression.It was suggested that the AaHsp90 protein may play an important role in the process of adaptation to high temperature and starvation stress in A.assama.
文摘三阴性乳腺癌(triple negative breast cancer,TNBC)恶性程度高,预后差,其p53基因的突变频率达80%以上。日蟾蜍它灵(gamabufotalin,CS-6)是日本蟾蜍的皮肤分泌物,有研究表明CS-6具有抗癌作用,但其对TNBC的作用和机制尚无报道。本文采用MTT法和细胞克隆形成实验检测了不同浓度CS-6对TNBC细胞系MDA-MB-468和MDA-MB-231细胞增殖的影响;采用Western-blot检测了CS-6在外源和内源水平对p53和热休克蛋白90(heat shock protein 90,HSP90)表达水平的影响;采用联合指数及等效线分析法考察了CS-6和HSP90的抑制剂坦螺旋霉素(tanespimycin,17-AAG)对MDA-MB-468和MDA-MB-231细胞增殖的协同作用。结果显示,CS-6明显抑制MDA-MB-468和MDA-MB-231细胞的增殖,其抑制增殖机制有可能与降解HSP90/突变p53有关;而且,CS-6和HSP90抑制剂17-AAG联合用药对抑制MDA-MB-468和MDA-MB-231细胞增殖具有协同作用。
文摘为研究猪精液热休克蛋白90(heat-shock protein 90,Hsp90)的定位以及表达特性,通过RT-PCR、蛋白质印迹、免疫组化、免疫荧光方法,检测和分析Hsp90在猪繁殖组织(睾丸和卵巢)、生殖细胞(精子细胞和卵母细胞)中的定位以及表达.RT-PCR结果显示,Hsp90在睾丸和附睾尾表达,在卵巢中微量表达;蛋白质印迹结果显示,Hsp90蛋白在睾丸、卵巢、输卵管和尿道球腺中高表达;免疫组化结果显示,Hsp90蛋白在睾丸的间质细胞、次级精母细胞和精子细胞中表达,也在卵巢的卵母细胞、卵丘细胞和颗粒细胞中表达;免疫荧光结果显示,Hsp90蛋白定位在精子的颈部、尾中部、卵丘细胞的细胞质和卵母细胞中.综上所述,Hsp90在猪主要的繁殖组织和生殖细胞中高表达,其表达水平可能与精子质量和胚胎发育有关.
基金funding support from the National Natural Science Foundation of China(Grant Nos.81672467,81702773,81702389,and 81672368)the Major National R&D Project(Grant Nos.2018ZX10723204,2018ZX10302205,and 2018ZX09J18107)the Natural Science Foundation of Beijing(Grant No.7172207)。
文摘Objective:Hepatocellular carcinoma(HCC),the main type of liver cancer,has a high morbidity and mortality,and a poor prognosis.RNA helicase DDX5,which acts as a transcriptional co-regulator,is overexpressed in most malignant tumors and promotes cancer cell growth.Heat shock protein 90(HSP90)is an important molecular chaperone in the conformational maturation and stabilization of numerous proteins involved in cell growth or survival.Methods:DDX5 m RNA and protein expression in surgically resected HCC tissues from 24 Asian patients were detected by quantitative real-time PCR and Western blot,respectively.The interaction of DDX5-HSP90 was determined by molecular docking,immunoprecipitation,and laser scanning confocal microscopy.The autophagy signal was detected by Western blot.The cell functions and signaling pathways of DDX5 were determined in 2 HCC cell lines.Two different murine HCC xenograft models were used to determine the function of DDX5 and the therapeutic effect of an HSP90 inhibitor.Results:HSP90 interacted directly with DDX5 and inhibited DDX5 protein degradation in the AMPK/ULK1-regulated autophagy pathway.The subsequent accumulation of DDX5 protein induced the malignant phenotype of HCC by activating theβ-catenin signaling pathway.The silencing of DDX5 or treatment with HSP90 inhibitor both blocked in vivo tumor growth in a murine HCC xenograft model.High levels of HSP90 and DDX5 protein were associated with poor prognoses.Conclusions:HSP90 interacted with DDX5 protein and subsequently protected DDX5 protein from AMPK/ULK1-regulated autophagic degradation.DDX5 and HSP90 are therefore potential therapeutic targets for HCC.
文摘<abstract>Aim: To study the protein changes of spermatozoa associated with sperm motility during sperm cryopreservation and its mechanism. Methods: In 18 healthy men, the seminal sperm motility and HSP90 levels were studied before and after cryopreservation using SDS-PAGE, Western blotting and computerized image analysis. Results: The sperm motility declined significantly after cryopreservation (P<0.01). The average grey level and the integrated grey level of sperm HSP90 before cooling were 34.1±3.2 and 243.0±21.6, respectively, while those after thawing were 23.2±2.5 and 105.7±28.5, respectively. Both parameters were decreased significantly (P<0.01). No HSP90 was found in the seminal plasma before and after cryopreservation. Conclusion: HSP90 in human spermatozoa was decreased substantially after cryopreservation. This may result from protein degradation, rather than leakage into the seminal plasma.
基金financially supported by the Deanship of Research at Jordan University of Science and Technology,Irbid,Jordan
文摘Previous studies have confirmed that heat shock protein 90 overexpression can lead to dopami- nergic neuronal death. This study was designed to further investigate what effects are produced by heat shock protein 90 after endurance exercise training. Immunohistochemistry results showed that exercise training significantly inhibited heat shock protein 90 overexpression in the soleus and gastrocnemius in Parkinson＇s disease rats, which is a potential therapeutic target for ameliorating skeletal muscle abnormalities in Parkinso^s disease.
