The purpose of this study was to test the hypothesis that the combination of micro-arc oxidation and alkali heatment （MAH） would improve the cytocompatibility of a newly designed Ti-24Nb-4Zr-8Sn alloy. In this study...The purpose of this study was to test the hypothesis that the combination of micro-arc oxidation and alkali heatment （MAH） would improve the cytocompatibility of a newly designed Ti-24Nb-4Zr-8Sn alloy. In this study, commercially pure titanium （cp Ti） and Ti-24Nb-4Zr-8Sn were used. Surface modification of Ti-24Nb- 4Zr-8Sn by a two-step treatment of micro-arc oxidation （MAO） and alkali heatment was reported. Surface characterizations were performed by scanning electron microscopy （SEM）, thin film X-ray diffraction （TF-XRD） and X-ray photoelectron spectroscopy （XPS）. The MAH layer consisted of finer crystals and possessed a higher degree of crystallity and stability than the MAO layer. A biocompatibility study on treated and untreated Ti- 24Nb-4Zr-8Sn in comparison with cp Ti was carried out to investigate the effect of the different surfaces on the bone integration property in vitro. The cellular assays revealed that the MAO and MAH layer favored the initial adhesion of MC3T3-E1 cells and that the growth rate of MC3T3-E1 cells on MAH layer was significantly higher than that on the conventional MAO-treated layer after 3-day and 5-day incubation, demonstrating the greater potential of the hybrid treatment of micro-arc oxidation followed with alkali heatment as a novel surface modification method for implanting materials.展开更多
目的:探讨经微弧氧化碱热处理(Micro-arc oxidation and alkali heatment,MAH)后Ti-24Nb-4Zr-8Sn合金和Bio-Oss修复种植体周围骨缺损的愈合情况。方法:Ti-24Nb-4Zr-8Sn合金制备成圆柱状样本,分二组(光滑表面和微弧氧化碱热组)进行表面...目的:探讨经微弧氧化碱热处理(Micro-arc oxidation and alkali heatment,MAH)后Ti-24Nb-4Zr-8Sn合金和Bio-Oss修复种植体周围骨缺损的愈合情况。方法:Ti-24Nb-4Zr-8Sn合金制备成圆柱状样本,分二组(光滑表面和微弧氧化碱热组)进行表面处理。拔除犬下颌第二、三、四前磨牙,拔牙窝周围制备骨缺损,将两组种植体随机植入拔牙窝内,缺损区放置Bio-Oss,1个月后组织学染色观察种植体周围骨缺损的修复情况。结果:微弧氧化碱热种植体周围及Bio-Oss颗粒表面类骨质多而密,种植体表面可见大量新生骨的存在,BIC达25.68±5.2%。光滑表面种植体周围的骨缺损区,Bio-Oss颗粒周围新生骨较少,种植体表面未见新生骨的存在。结论:MAH和Bio-Oss可促进种植体周围骨缺损的修复及种植体骨整合。展开更多
基金supported by the National Natural Science Foundation of China under grant No. 30973354
文摘The purpose of this study was to test the hypothesis that the combination of micro-arc oxidation and alkali heatment （MAH） would improve the cytocompatibility of a newly designed Ti-24Nb-4Zr-8Sn alloy. In this study, commercially pure titanium （cp Ti） and Ti-24Nb-4Zr-8Sn were used. Surface modification of Ti-24Nb- 4Zr-8Sn by a two-step treatment of micro-arc oxidation （MAO） and alkali heatment was reported. Surface characterizations were performed by scanning electron microscopy （SEM）, thin film X-ray diffraction （TF-XRD） and X-ray photoelectron spectroscopy （XPS）. The MAH layer consisted of finer crystals and possessed a higher degree of crystallity and stability than the MAO layer. A biocompatibility study on treated and untreated Ti- 24Nb-4Zr-8Sn in comparison with cp Ti was carried out to investigate the effect of the different surfaces on the bone integration property in vitro. The cellular assays revealed that the MAO and MAH layer favored the initial adhesion of MC3T3-E1 cells and that the growth rate of MC3T3-E1 cells on MAH layer was significantly higher than that on the conventional MAO-treated layer after 3-day and 5-day incubation, demonstrating the greater potential of the hybrid treatment of micro-arc oxidation followed with alkali heatment as a novel surface modification method for implanting materials.
文摘目的:探讨经微弧氧化碱热处理(Micro-arc oxidation and alkali heatment,MAH)后Ti-24Nb-4Zr-8Sn合金和Bio-Oss修复种植体周围骨缺损的愈合情况。方法:Ti-24Nb-4Zr-8Sn合金制备成圆柱状样本,分二组(光滑表面和微弧氧化碱热组)进行表面处理。拔除犬下颌第二、三、四前磨牙,拔牙窝周围制备骨缺损,将两组种植体随机植入拔牙窝内,缺损区放置Bio-Oss,1个月后组织学染色观察种植体周围骨缺损的修复情况。结果:微弧氧化碱热种植体周围及Bio-Oss颗粒表面类骨质多而密,种植体表面可见大量新生骨的存在,BIC达25.68±5.2%。光滑表面种植体周围的骨缺损区,Bio-Oss颗粒周围新生骨较少,种植体表面未见新生骨的存在。结论:MAH和Bio-Oss可促进种植体周围骨缺损的修复及种植体骨整合。
