缺氧-缺血性脑病新生儿脐血HSP70、HIF-1a水平相关性研究

目的:探讨缺氧缺血性脑病(HIE)新生儿脐血热休克蛋白70(HSP70)、缺氧诱导因子-1a(HIF-1a)水平的变化。方法:选择足月HIE新生儿30例(轻中度HIE组20例,重度HIE组10例),另选同期出生的20例足月健康新生儿作为对照组,采用酶联免疫吸附法测定脐血HSP70、HIF-1a水平。结果:轻中度、重度HIE组新生儿脐血HSP70、HIF-1a水平与对照组比较,差异有统计学意义(P<0.01);轻中度、重度HIE组组间比较,差异有统计学意义(P<0.01);HIE患儿脐血HSP70、HIF-1a水平呈显著正相关(r=0.833,P<0.01)。结论:HIE患儿脐血HSP70、HIF-1a水平与HIE的病理生理过程密切相关,有望作为病情程度的判断指标之一,对临床诊断及预后的判断具有一定的指导意义。

Correlation between heat shock protein 70 expression in the brain stem and sudden death after experimental traumatic brain injury

Objective:Theaimof thisstudywas to determinethepatternsof heat-shockprotein70(HSP 70 )biosynthesis followingtraumaticbraininjury,andobservetheeffectof HSP 70 inductionon thefunctionof thevitalcenterinthebrain stem.Methods:Ratmodelsof suddendeathresultedformtraumaticbraininjurywereproduced,andHSP 70 expressionin theratbrainstemwasdeterminedby immunohistochemistry,theinductionof HSP 70 mRNAdetectedby RT-PCR.Re -sults: Thelevelof HSP 70 mRNAwasprominentlyelevatedinthebrainstemas earlyas15minfollowingtheimpactin-jury,whileHSP 70 expressionwasonlyobserved3to6h aftertheinjury.Itwasalsoobservedthatthelevelsof HSP 70 mR-NAbutnottheproteinwereelevatedinthebrainstemof suddendeathrats.Conclusion:Thesynthesisof HSP 70 wassig-nificantlyenhancedinthebrainstemfollowingtraumaticinjury,andtheexpressionof HSP 70 is beneficialto eliminatethe stressagents,andto sustainthecellularproteinhomeostasis.Whentheinjurydisturbsthesynthesisof HSP 70 to disarmthe protectivemechanismof heat-shockproteins,dysfunctionof thevitalcenterinthebrainstem,andconsequentlydeathmay occur.Breachinthesynchronizationof HSP 70 mRNA-proteincanbeindicativeof fataldamageto thenervecells.