目的:本文旨在研究胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)D442G基因多态性与脑梗死发病的相关性。方法:选取包头医学院第一附属医院神经内科脑梗死患者180例为观察组,体检科体检人员130例为对照组。DNA试剂盒提取...目的:本文旨在研究胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)D442G基因多态性与脑梗死发病的相关性。方法:选取包头医学院第一附属医院神经内科脑梗死患者180例为观察组,体检科体检人员130例为对照组。DNA试剂盒提取外周血DNA,采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorph,PCR-RFLP)方法检测CETP D442G的基因型,分析D442G基因型与脑梗死发病的关系。结果:D442G基因位点DG基因型高密度脂蛋白水平高于GG、DD基因型,差异有统计学意义(P<0.05)。以是否发生脑梗死作为应变量,以年龄、性别、体重指数、血脂水平、糖尿病、高血压、心脏病、CETP D442G基因型及等位基因频率作为自变量,应用非条件logistic回归分析得出年龄、高血压、糖尿病、甘油三酯、胆固醇、低密度脂蛋白为脑梗死发病的独立危险因素(OR>1,P<0.05);高密度脂蛋白、DG基因型为脑梗死发病的保护因素(OR<1,P<0.05)。结论:脑梗死发病的危险因素可能为年龄、吸烟史、高血压病史、糖尿病病史、胆固醇、低密度脂蛋白、甘油三酯水平,脑梗死发病的保护因素可能为D442G基因DG基因型及高密度脂蛋白水平。展开更多
Primary central nervous system lymphomas (PCNSL) frequently reveal genomic instability. We analysed different functional genetic variants affecting the folate and homocysteine metabolism important for DNA integrity in...Primary central nervous system lymphomas (PCNSL) frequently reveal genomic instability. We analysed different functional genetic variants affecting the folate and homocysteine metabolism important for DNA integrity in 31 PCNSL patients and 142 controls. We found significantly less carriers of the methionine synthase c. 2756A> G (D919G) missense polymorphism among the patients (0.16 vs 0.42; odds ratio 0.26, CI(95%): 0.09-0.74; P=0.005), suggesting a protective function of the G allele. These data stimulate further epidemiological and functional studies focusing on the role of homoeysteine and folate metabolism in lymphoma tumorigenesis.展开更多
文摘Primary central nervous system lymphomas (PCNSL) frequently reveal genomic instability. We analysed different functional genetic variants affecting the folate and homocysteine metabolism important for DNA integrity in 31 PCNSL patients and 142 controls. We found significantly less carriers of the methionine synthase c. 2756A> G (D919G) missense polymorphism among the patients (0.16 vs 0.42; odds ratio 0.26, CI(95%): 0.09-0.74; P=0.005), suggesting a protective function of the G allele. These data stimulate further epidemiological and functional studies focusing on the role of homoeysteine and folate metabolism in lymphoma tumorigenesis.
