AIM： To investigate the anti-inflammatory effect of an aqueous whole plant extract of Heliotropium indicum（HIE） on endotoxin-induced uveitis in New Zealand white rabbits.·METHODS： Clinical signs of uveitis in...AIM： To investigate the anti-inflammatory effect of an aqueous whole plant extract of Heliotropium indicum（HIE） on endotoxin-induced uveitis in New Zealand white rabbits.·METHODS： Clinical signs of uveitis including flares,iris hyperemia and miosis, were sought for and scored in1.0 mg/kg lipopolysaccharide（LPS）-induced uveitic rabbits treated orally with HIE（30-300 mg/kg）,prednisolone（30 mg/kg）, or normal saline（10 m L/kg）. The number of polymorphonuclear neutrophils infiltrating, the protein concentration, as well as levels of tumor necrosis factor-α（TNF-α）, prostaglandin E2（PGE2）, and monocyte chemmoattrant protein-1（MCP-1） in the aqueous humor after the various treatments were also determined. A histopathological study of the anterior uveal was performed.· RESULTS： The extract and prednisolone-treatment significantly reduced（P ≤0.001） both the clinical scores of inflammation（1.0-1.8 compared to 4.40 ±0.40 in the normal saline-treated rabbits） and inflammatory cells infiltration. The level of protein, and the concentrationsof TNF-α, PGE2 and MCP-1 in the aqueous humor were also significantly reduced（P ≤0.001）. Histopathological studies showed normal uveal morphology in the HIE and prednisolone-treated rabbits while normal saline-treated rabbits showed marked infiltration of inflammatory cells.· CONCLUSION： The HIE exhibits anti-inflammatory effect on LPS-induced uveitis possibly by reducing the production of pro-inflammatory mediators.展开更多
Genus Heliotropium L. (Heliotropiaceae) in south west of Saudi Arabia has been studied;seven species of H. arbainense Fresen, H. longiflorum (D.C.) Jauber & Spach, H. petrocarpum Hockst & Steud, H. strigosium ...Genus Heliotropium L. (Heliotropiaceae) in south west of Saudi Arabia has been studied;seven species of H. arbainense Fresen, H. longiflorum (D.C.) Jauber & Spach, H. petrocarpum Hockst & Steud, H. strigosium Willd, H. zeylanicum Lam., H. jizanense Al-Turki and H. lasiocarpum Fisch were collected, recognized, typed and nomenclatured. The most valuable characters were those of macro and distinct micromorphological data such as stomata, hairs, pollen grains and stem anatomy. Light microscopy has been used in these studies. From the obtained results, trichomes and pollen grains data in addition to stem anatomy indicated good taxonomic tools to differentiate between species of this genus. Different traditional measurements were strikingly also helpful for the discrimination of species. H. longiflorum was characterized by distinctive data such as prominent anatomical information, P/E (1.8 μm) in addition to presence of spindle hair which in turn can be separated as a taxonomic level. Also, the results revealed a complete affinity between the two species of H. jizanense and H. lasiocarpum. An artificial key is provided for the differentiation between the studied species.展开更多
Heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conserva...Heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conservation of this plant applying tissue culture method was used in the present study. In vitro plant regeneration has been established via shoot organogenesis using nodal meristems. The effect of various plant growth regulators was evaluated to regenerate the whole plants by in vitro micropropagation in modified MS media supplemented with different plant growth regulators (PGRs). In vitro regeneration of plants was involved in three steps by shoot initiation, multiplication and rooting of microshoots. Initially 100% explants of Heliotropium kotschyi responded to initiate shoot in MS medium supplemented with 8.88 μM BAP with 5.71 μM IAA after 4 weeks of culture. Multiplication of shoots and ultimate plant regeneration capacity of Heliotropium kotschyi were compared and the highest performance of 578 shoots/explant was observed in presence of 8.88 μM BAP and 5.71 μM IAA after second transfer. Rooted plantlets were transferred to soil pots and 60% plants were survived after one and a half months. The highly efficient plant regeneration protocol of the desert plant is of great value in rapid plant propagation program for the purpose of conservation of biodiversity.展开更多
Objective:To investigate the clot lysis,antimicrobic and membrane stabilizing potentials of ethanolic extractives of the leaves of Heliotropium indicum(H.indicum).Methods:Crude ethanolic extracts of H.indicum leaves w...Objective:To investigate the clot lysis,antimicrobic and membrane stabilizing potentials of ethanolic extractives of the leaves of Heliotropium indicum(H.indicum).Methods:Crude ethanolic extracts of H.indicum leaves were partitioned successively using solvents of different polarity and subjected to determine qualitatively and quantitatively for the presence of different bioactive constitutes and fractions which were assessed for their possible clot lysis,antimicrobic,and membrane stabilizing activities as compared with the known drugs.Results:For the thrombolytic activity,the petroleum ether soluble fraction showed the highest percent of clot lysis(35.4%)among all fractions,while streptokinase and water resulted in 65.15%and 3.77%clot lysis,respectively.With respect to the membrane stabilizing activity,carbon tetrachloride soluble fraction of H.indicum profoundly interdicted the hemolysis of erythrocytes brought about by osmotic induction(39.24%)or by heat(40.20%).The other fractions exhibited less significant membrane stabilizing effect.By contrast,acetylsalicylic acid resulted in 72.25%±0.30%inhibition of osmotically induced hemolysis and showed a lower level of protection of heat induced hemolysis(42.56%).Crude ethanolic extracts were moderately sensitive against known pathogenic microbes.Since pharmacological activities of H.indicum are due to the presence of bioactive compounds,we detected and quantified the presence of significant levels of flavonoid and tannin substances.Conclusions:The outcomes of this research show that the leaves of H.indicum have the potential to be used as a remedy for thrombosis,inflammatory diseases and against few important bacterial pathogens.展开更多
文摘AIM： To investigate the anti-inflammatory effect of an aqueous whole plant extract of Heliotropium indicum（HIE） on endotoxin-induced uveitis in New Zealand white rabbits.·METHODS： Clinical signs of uveitis including flares,iris hyperemia and miosis, were sought for and scored in1.0 mg/kg lipopolysaccharide（LPS）-induced uveitic rabbits treated orally with HIE（30-300 mg/kg）,prednisolone（30 mg/kg）, or normal saline（10 m L/kg）. The number of polymorphonuclear neutrophils infiltrating, the protein concentration, as well as levels of tumor necrosis factor-α（TNF-α）, prostaglandin E2（PGE2）, and monocyte chemmoattrant protein-1（MCP-1） in the aqueous humor after the various treatments were also determined. A histopathological study of the anterior uveal was performed.· RESULTS： The extract and prednisolone-treatment significantly reduced（P ≤0.001） both the clinical scores of inflammation（1.0-1.8 compared to 4.40 ±0.40 in the normal saline-treated rabbits） and inflammatory cells infiltration. The level of protein, and the concentrationsof TNF-α, PGE2 and MCP-1 in the aqueous humor were also significantly reduced（P ≤0.001）. Histopathological studies showed normal uveal morphology in the HIE and prednisolone-treated rabbits while normal saline-treated rabbits showed marked infiltration of inflammatory cells.· CONCLUSION： The HIE exhibits anti-inflammatory effect on LPS-induced uveitis possibly by reducing the production of pro-inflammatory mediators.
文摘Genus Heliotropium L. (Heliotropiaceae) in south west of Saudi Arabia has been studied;seven species of H. arbainense Fresen, H. longiflorum (D.C.) Jauber & Spach, H. petrocarpum Hockst & Steud, H. strigosium Willd, H. zeylanicum Lam., H. jizanense Al-Turki and H. lasiocarpum Fisch were collected, recognized, typed and nomenclatured. The most valuable characters were those of macro and distinct micromorphological data such as stomata, hairs, pollen grains and stem anatomy. Light microscopy has been used in these studies. From the obtained results, trichomes and pollen grains data in addition to stem anatomy indicated good taxonomic tools to differentiate between species of this genus. Different traditional measurements were strikingly also helpful for the discrimination of species. H. longiflorum was characterized by distinctive data such as prominent anatomical information, P/E (1.8 μm) in addition to presence of spindle hair which in turn can be separated as a taxonomic level. Also, the results revealed a complete affinity between the two species of H. jizanense and H. lasiocarpum. An artificial key is provided for the differentiation between the studied species.
文摘Heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conservation of this plant applying tissue culture method was used in the present study. In vitro plant regeneration has been established via shoot organogenesis using nodal meristems. The effect of various plant growth regulators was evaluated to regenerate the whole plants by in vitro micropropagation in modified MS media supplemented with different plant growth regulators (PGRs). In vitro regeneration of plants was involved in three steps by shoot initiation, multiplication and rooting of microshoots. Initially 100% explants of Heliotropium kotschyi responded to initiate shoot in MS medium supplemented with 8.88 μM BAP with 5.71 μM IAA after 4 weeks of culture. Multiplication of shoots and ultimate plant regeneration capacity of Heliotropium kotschyi were compared and the highest performance of 578 shoots/explant was observed in presence of 8.88 μM BAP and 5.71 μM IAA after second transfer. Rooted plantlets were transferred to soil pots and 60% plants were survived after one and a half months. The highly efficient plant regeneration protocol of the desert plant is of great value in rapid plant propagation program for the purpose of conservation of biodiversity.
基金Supported by the assistance of research section of Department of Pharmacy,World University of Bangladesh(Grant No.WUBPS#04567).
文摘Objective:To investigate the clot lysis,antimicrobic and membrane stabilizing potentials of ethanolic extractives of the leaves of Heliotropium indicum(H.indicum).Methods:Crude ethanolic extracts of H.indicum leaves were partitioned successively using solvents of different polarity and subjected to determine qualitatively and quantitatively for the presence of different bioactive constitutes and fractions which were assessed for their possible clot lysis,antimicrobic,and membrane stabilizing activities as compared with the known drugs.Results:For the thrombolytic activity,the petroleum ether soluble fraction showed the highest percent of clot lysis(35.4%)among all fractions,while streptokinase and water resulted in 65.15%and 3.77%clot lysis,respectively.With respect to the membrane stabilizing activity,carbon tetrachloride soluble fraction of H.indicum profoundly interdicted the hemolysis of erythrocytes brought about by osmotic induction(39.24%)or by heat(40.20%).The other fractions exhibited less significant membrane stabilizing effect.By contrast,acetylsalicylic acid resulted in 72.25%±0.30%inhibition of osmotically induced hemolysis and showed a lower level of protection of heat induced hemolysis(42.56%).Crude ethanolic extracts were moderately sensitive against known pathogenic microbes.Since pharmacological activities of H.indicum are due to the presence of bioactive compounds,we detected and quantified the presence of significant levels of flavonoid and tannin substances.Conclusions:The outcomes of this research show that the leaves of H.indicum have the potential to be used as a remedy for thrombosis,inflammatory diseases and against few important bacterial pathogens.
