To compare the early effects of hypertonic and isotonic saline resuscitation on heme oxygenase-1 （HO-1） expression in organs of rats with hemorrhagic shock. Rats were randomly divided into hypertonic saline resuscit...To compare the early effects of hypertonic and isotonic saline resuscitation on heme oxygenase-1 （HO-1） expression in organs of rats with hemorrhagic shock. Rats were randomly divided into hypertonic saline resuscitation （HTS）, normal saline resuscitation （NS） and sham groups. HO-1 mRNA, protein expression and apoptosis were evaluated in organs. In the HTS group, significant difference was noted in HO-1 protein in small intestinal mucosa and liver compared with the NS and sham groups, and in HO-1 mRNA in liver and kidney compared with the sham group. The apoptosis of small intestinal mucosa, liver, heart, and lung was significantly lower in the HTS group than that in the NS group. In this study, small volume resuscitation with HTS can efficiently up-regulate the expression level of HO-1 in small intestinal mucosa and liver, which may be one of the mechanisms alleviating organ damage.展开更多
＇Core＇ neuropathology of degenerative central nervous system （CNS） disorders The common human neurodegenerative disorders （Alzheimer＇s disease （AD）, Parkinson＇s disease （PD）, amyotrophic lateral sclerosis, ...＇Core＇ neuropathology of degenerative central nervous system （CNS） disorders The common human neurodegenerative disorders （Alzheimer＇s disease （AD）, Parkinson＇s disease （PD）, amyotrophic lateral sclerosis, etc.） vary with respect to risk factors, ages of onset, sex predilections, neuraxial regions affected, hallmark cellular inclusions, behavioral and neurological symptoms, and responses to treatment. Despite these differences, there appears to be a set of ＇core＇ neuropathological features shared among these and related entities. Common to these conditions are 1） pathological deposition of non-transferrin bound iron, 2） oxidative stress and associated protein, lipid and nucleic acid modifications, 3） mitochondrial membrane damage and bioenergetic failure, and 4） macroautophagy in the affected neural tissues.展开更多
目的:分析血清人新饱食分子蛋白-1(nesfatin-1)、爱帕琳肽(apelin)、血红素氧合酶-1(HO-1)水平与糖尿病视网膜病变(DR)严重程度的相关性。方法:选取2020-09/2022-09我院收治的2型糖尿病(T2DM)患者100例,根据眼底病变情况分为无DR(NDR)组...目的:分析血清人新饱食分子蛋白-1(nesfatin-1)、爱帕琳肽(apelin)、血红素氧合酶-1(HO-1)水平与糖尿病视网膜病变(DR)严重程度的相关性。方法:选取2020-09/2022-09我院收治的2型糖尿病(T2DM)患者100例,根据眼底病变情况分为无DR(NDR)组(35例)、NPDR组(33例)、PDR组(32例)。另选取同期于本院进行健康体检者30例作为对照组。检测并分析各组研究对象血清nesfatin-1、apelin、HO-1水平,评估DR患者的全视网膜缺血指数(ISI)。结果:T2DM患者血清nesfatin-1、HO-1水平均低于对照组,apelin水平均高于对照组,且PDR组血清nesfatin-1、HO-1水平最低,而apelin水平最高。PDR组全视网膜ISI高于NPDR组(4.56±0.57 vs 2.05±0.29,P<0.05)。相关性分析显示,DR患者血清nesfatin-1、HO-1水平与全视网膜ISI均呈负相关,而apelin水平与全视网膜ISI呈正相关。受试者工作特征曲线(ROC)分析显示,血清nesfatin-1、apelin、HO-1预测PDR曲线下面积分别为0.842、0.833、0.807。结论:血清nesfatin-1、apelin、HO-1水平与DR严重程度有密切关系,动态监测血清nesfatin-1、apelin、HO-1水平对及早发现PDR具有重要意义。展开更多
基金supported by the Zhejiang Provincial Natural Science Foundation of China (No.Y2100430)the Zhejiang Provincial Education and Research Foundation of China (No. Y201019154)
文摘To compare the early effects of hypertonic and isotonic saline resuscitation on heme oxygenase-1 （HO-1） expression in organs of rats with hemorrhagic shock. Rats were randomly divided into hypertonic saline resuscitation （HTS）, normal saline resuscitation （NS） and sham groups. HO-1 mRNA, protein expression and apoptosis were evaluated in organs. In the HTS group, significant difference was noted in HO-1 protein in small intestinal mucosa and liver compared with the NS and sham groups, and in HO-1 mRNA in liver and kidney compared with the sham group. The apoptosis of small intestinal mucosa, liver, heart, and lung was significantly lower in the HTS group than that in the NS group. In this study, small volume resuscitation with HTS can efficiently up-regulate the expression level of HO-1 in small intestinal mucosa and liver, which may be one of the mechanisms alleviating organ damage.
基金supported by grants from the Canadian Institutes of Health Researchthe Mary Katz Claman Foundationthe Oberfeld Family Fund for Alzheimer Research
文摘＇Core＇ neuropathology of degenerative central nervous system （CNS） disorders The common human neurodegenerative disorders （Alzheimer＇s disease （AD）, Parkinson＇s disease （PD）, amyotrophic lateral sclerosis, etc.） vary with respect to risk factors, ages of onset, sex predilections, neuraxial regions affected, hallmark cellular inclusions, behavioral and neurological symptoms, and responses to treatment. Despite these differences, there appears to be a set of ＇core＇ neuropathological features shared among these and related entities. Common to these conditions are 1） pathological deposition of non-transferrin bound iron, 2） oxidative stress and associated protein, lipid and nucleic acid modifications, 3） mitochondrial membrane damage and bioenergetic failure, and 4） macroautophagy in the affected neural tissues.
文摘目的:分析血清人新饱食分子蛋白-1(nesfatin-1)、爱帕琳肽(apelin)、血红素氧合酶-1(HO-1)水平与糖尿病视网膜病变(DR)严重程度的相关性。方法:选取2020-09/2022-09我院收治的2型糖尿病(T2DM)患者100例,根据眼底病变情况分为无DR(NDR)组(35例)、NPDR组(33例)、PDR组(32例)。另选取同期于本院进行健康体检者30例作为对照组。检测并分析各组研究对象血清nesfatin-1、apelin、HO-1水平,评估DR患者的全视网膜缺血指数(ISI)。结果:T2DM患者血清nesfatin-1、HO-1水平均低于对照组,apelin水平均高于对照组,且PDR组血清nesfatin-1、HO-1水平最低,而apelin水平最高。PDR组全视网膜ISI高于NPDR组(4.56±0.57 vs 2.05±0.29,P<0.05)。相关性分析显示,DR患者血清nesfatin-1、HO-1水平与全视网膜ISI均呈负相关,而apelin水平与全视网膜ISI呈正相关。受试者工作特征曲线(ROC)分析显示,血清nesfatin-1、apelin、HO-1预测PDR曲线下面积分别为0.842、0.833、0.807。结论:血清nesfatin-1、apelin、HO-1水平与DR严重程度有密切关系,动态监测血清nesfatin-1、apelin、HO-1水平对及早发现PDR具有重要意义。
