Diwu Yanggan Modulates the Wnt/β-catenin Pathway and Inhibits Liver Carcinogenesis Signaling in 2-AAF/PH Model Rats

The activation of the Wnt/β-catenin signaling pathway in oval cells after liver injury is implicated in hepatocarcinogenesis.Diwu Yanggan capsule is a Chinese herbal medicine that has been used for treating liver disorder.The present study aimed to examine the mechanism by which Diwu Yanggan inhibits liver carcinogenesis,and the involvement of the Wnt/β-catenin signaling pathway.Diwu Yanggan capsule was administered to 2-acetaminofluorene/partial hepatectomy(2-AAF/PH)rats,a murine model of liver injury.The biomarkers of oval cells and key proteins in the Wnt/p-catenin signaling pathway were assessed on postoperative day 8,10,14,17,19 and 22.The results showed that treatment with Diwu Yanggan was associated with reduced expression of oval cell and stem cell biomarkers in the 2-AAF/PH animals.The expression pattern of key proteins in the Wnt/β-catenin pathway was altered in Diwu Yanggan-treated animals,indicating that the Diwu Yanggan treatment accelerated the activation of the Wnt/β-catenin pathway in the initial stage and contributed to its deactivation in the later stage.Histological findings indicated that hepatocyte proliferation was suppressed in Diwu Yanggan-treated animals,compared with untreated 2-AAF/PH animals.Taken together,Diwu Yanggan capsule may reduce the risk of hepatocarcinogenesis by modulating the Wnt/β-catenin signaling pathway.

地五养肝胶囊对2-AAF/PH大鼠模型肝癌前病变的抑制作用

目的观察地五养肝胶囊(DWYG)对2-AAF/PH大鼠模型肝癌前病变的抑制作用。方法建立2-AAF/PH大鼠肝癌前病变模型,采用SPF级Wistar大鼠384只,随机分为空白组、假手术组、模型组、DWYG组。取肝切除后第8、10、14、17、19、22天实验大鼠肝组织进行病理组织学观察,采用IPP6.0软件进行图像处理。计数各实验点肝脏表面直径>1mm的小结节、细胞核数目、细胞核面积总量、细胞核平均直径和细胞核IOD值。结果肝组织结节数:术后第8、22天,DWYG组低于模型组。细胞核数目:术后第10、14、17、22天,DWYG组高于空白组。细胞核面积总量:术后第17天,DWYG组高于空白组;术后第19天,DWYG组低于模型组。细胞核平均直径:术后第17天,DWYG组高于空白组,术后第19天,DWYG组低于模型组。以上实验数据经统计学处理,差异显著,P<0.01或P<0.05。结论地五养肝胶囊具有抑制2-AAF/PH模型大鼠肝癌前病变的作用。