By using Fab'-enzyme labelled immuno absorbent assay (ELISA) with sensitivity of picogram (10-12 g or pg) level, the M-type pyruvate kinase (M-PyK) in plasma was determined in 47 cases of normal healthy adult and ...By using Fab'-enzyme labelled immuno absorbent assay (ELISA) with sensitivity of picogram (10-12 g or pg) level, the M-type pyruvate kinase (M-PyK) in plasma was determined in 47 cases of normal healthy adult and 26 cases of hepatocellular carcinoma (HCC) patient. It was found that the upper limits of normal male and female were 1.1 and 1.4 ng/ml (expressed as M2-PyK) respectively. The plasma M-PyK in HCC patients was significant increased to above 5 times of average normal level, the positive rate was about 95%. In 6 cases of subclinical small hepatocarcinoma and 7 cases of HCC patient with normal serum alpha-fetal protein level, the mean plasma M-PyK value was also increased. Whereas the plasma M-PyK level in acute or chronic hepatitis and other benign diseases were normal. After the HCC being resected, the plasma M-PyK returned to normal, but increased again in the cases of recurrent hepatocarcinoma, suggesting that the increased M-PyK in the plasma of HCC patient was criginated from M2-type PyK in HCC tissue. Therefore, plasma M-PyK may become a new micro-level index of hepatocarcinoma.展开更多
Copper contents(CU) in bodies and serum cerulopasmin(Cp) were assayed in patients with liver cirrhosis(LC) and hepatocarcinoma(HCC) with atomic absorption and other methods.
目的通过观察肝脏B病毒x蛋白（hepatitis B virus X protein，HBX）和巨噬细胞移动抑制因子（macrophage migration inhibitory factor，MIF）在原发性肝癌（HCC）中的表达，结合临床资料探讨它们和HCC之间的关系。方法用免疫组织化学...目的通过观察肝脏B病毒x蛋白（hepatitis B virus X protein，HBX）和巨噬细胞移动抑制因子（macrophage migration inhibitory factor，MIF）在原发性肝癌（HCC）中的表达，结合临床资料探讨它们和HCC之间的关系。方法用免疫组织化学法检测40例HCC组织中HBX和MIF表达，并将上述检测结果结合I晦床资料进行相关性分析。结果40例癌组织中25例呈HBX高表达，22例呈MIF高表达，与低表达组比较均有统计学意义；HBX和MIF表达高低与HCC病理分级和临床分期均呈正相关；MIF与HCC复发转移呈正相关。结论HBX和MIF在HCC正相关，联合应用HBX和MIF有可能作为判断HCC恶性程度的肿瘤标志物。展开更多
文摘By using Fab'-enzyme labelled immuno absorbent assay (ELISA) with sensitivity of picogram (10-12 g or pg) level, the M-type pyruvate kinase (M-PyK) in plasma was determined in 47 cases of normal healthy adult and 26 cases of hepatocellular carcinoma (HCC) patient. It was found that the upper limits of normal male and female were 1.1 and 1.4 ng/ml (expressed as M2-PyK) respectively. The plasma M-PyK in HCC patients was significant increased to above 5 times of average normal level, the positive rate was about 95%. In 6 cases of subclinical small hepatocarcinoma and 7 cases of HCC patient with normal serum alpha-fetal protein level, the mean plasma M-PyK value was also increased. Whereas the plasma M-PyK level in acute or chronic hepatitis and other benign diseases were normal. After the HCC being resected, the plasma M-PyK returned to normal, but increased again in the cases of recurrent hepatocarcinoma, suggesting that the increased M-PyK in the plasma of HCC patient was criginated from M2-type PyK in HCC tissue. Therefore, plasma M-PyK may become a new micro-level index of hepatocarcinoma.
文摘Copper contents(CU) in bodies and serum cerulopasmin(Cp) were assayed in patients with liver cirrhosis(LC) and hepatocarcinoma(HCC) with atomic absorption and other methods.
文摘目的通过观察肝脏B病毒x蛋白（hepatitis B virus X protein，HBX）和巨噬细胞移动抑制因子（macrophage migration inhibitory factor，MIF）在原发性肝癌（HCC）中的表达，结合临床资料探讨它们和HCC之间的关系。方法用免疫组织化学法检测40例HCC组织中HBX和MIF表达，并将上述检测结果结合I晦床资料进行相关性分析。结果40例癌组织中25例呈HBX高表达，22例呈MIF高表达，与低表达组比较均有统计学意义；HBX和MIF表达高低与HCC病理分级和临床分期均呈正相关；MIF与HCC复发转移呈正相关。结论HBX和MIF在HCC正相关，联合应用HBX和MIF有可能作为判断HCC恶性程度的肿瘤标志物。
