[ Objective] The paper presents the effects of Chinese herbal extract on the performance of local chickens for its application in animal production. [Method] The herbal extract Ⅰ and Ⅱ were added to the basal diet o...[ Objective] The paper presents the effects of Chinese herbal extract on the performance of local chickens for its application in animal production. [Method] The herbal extract Ⅰ and Ⅱ were added to the basal diet of 10 day-old chickens at the ratio of 0.6%, respectively. Then the chickens was fasted and respectively weighed at 0, 17, 24 and 31 days old. And the feed consumption and mortality of each group were recorded to determine average weight, average daily gain, and feed conversion ratio. Economic benefit was also analyzed. [ Result] There was no significant difference in average daily gain between the test groups and the control group as well as between the two test groups （P〉0.05） ; there was signifi- cant difference in feed conversion ratio between the test groups and the control group （ P 〈0.05）, but no significant difference between the two test groups （ P 〉 0.05） ; the survival rate and the economic benefits of the test groups were significantly higher than that of control （ P 〈 0.05）. [ Conclusion ] Chinese herbal extract Ⅰ and Ⅱ should improve chicken average daily gain, feed conversion ratio and survival rate, promote growth, and signifi- cantly improve economic benefit.展开更多
The objective of this research was to investigate the effects of a natural herb extract on the general performance, carcass parameters and mortality of broiler chicks. A total of 720 one-day old chicks (Cobb 500) were...The objective of this research was to investigate the effects of a natural herb extract on the general performance, carcass parameters and mortality of broiler chicks. A total of 720 one-day old chicks (Cobb 500) were used in the experiment and randomly partitioned into nine pens (80 chicks per pen). Three experimental diets (C: regular starter feed;CC plus: regular starter feed supplemented with chicken plus herbal extract;AFC plus: antibiotic-free diet supplemented with chicken plus) were assigned each to three randomly selected pens. The herbal extract was supplemented in drinking water at rate of 300 ml/cubic meter. Results showed that weights, feed conversion ratios (FCR) and dressing percentages (DP) increased (P < 0.05) in birds supplemented with herb extract compared to control birds. Mortalities and sudden deaths were minimized via herbal supplementation. However, herbal supplementation had no significant effects on carcass cuts and some visceral organs. The relative economic efficiency (REE) was up to 13% improved by the herb supplementation. It can be concluded that feeding the herbal extract has significant positive effects on broilers general performance as feed efficiency is increased by 11% and on mortality and sudden death cases.展开更多
Recently reports showed that the pathobiology of acne vulgaris was arising from the exploration of sebaceous gland biology, hormonal factors, hyper-keratinization, and the role of bacteria, sebum, nutrition, cytokines...Recently reports showed that the pathobiology of acne vulgaris was arising from the exploration of sebaceous gland biology, hormonal factors, hyper-keratinization, and the role of bacteria, sebum, nutrition, cytokines and toll-like receptors (TLRs). Propionibacterium acnes (P.acnes) has a strong proinflammatory activity and targets molecules involved in the innate cutaneous immunity on keratinocytes by acting on TLR-2, leads to the development of comedones. GMP, a multi-herb extraction, targeted most of the major pathogenic features of acne with desired physicochemical traits. It strongly suppressed P.acnes growth, and reduced inflammation by suppressing the TLR-2/NF- kB pathway in SZ-95 sebocytes and HaCaT keratinocytes. GMP exhibited a marginal effect on cell viability and may have modulated hyper-keratinization of the epidermis. These results demonstrate the clinical feasibility of applying GMP for the treatment of acne.展开更多
Objective:To explore the anti-gout effects of four traditional Chinese medicine extracts of Eucommia,Sargentodoxa cuneata,Caulis spatholobi and Phellinus igniarius.Methods:Monosodium Urate(MSU)was used to establish a ...Objective:To explore the anti-gout effects of four traditional Chinese medicine extracts of Eucommia,Sargentodoxa cuneata,Caulis spatholobi and Phellinus igniarius.Methods:Monosodium Urate(MSU)was used to establish a mouse gouty arritis model.With coloricine as the positive control group,the effects of four traditional Chinese medicine extracts of Ecuador,Sargentodoxa,Caulis spatholobi and Phellinus igniarius on rice were studied;The hot plate method was used to observe the analytical effects of the four groups of traditional Chinese medicine extracts;the enzyme-linked immunoassay(ELISA)was used to detect residual interference-1 beta(IL-1 beta);the four traditional Chinese medicine extracts were simultaneously determined on the white blood cells,and monocytes in the blood of mice as well as the influence of neutrophils and histopathological changes of toes.Results:Compared with the normal group,the joint diameter ratio,blood leukocytes,monocytes,neutrophils,IL-1βat different time points in the model group were significantly increased(P<0.01),and the pain percentage was significantly reduced(P<0.05).Compared with the model group,the joint diameter ratio of the colchicine group,the Eucommia group,the Sargentodoxa group,the Caulis spatholobi group and the Phellinus sibiricum group was significantly reduced(P<0.05),and the pain percentage was significantly increased(P(0.05).The four groups of traditional Chinese medicine extracts significantly decreased the white blood cells,monocytes,and neutrophils in the blood of mice(P<0.05),and the IL-1βexpression levels of the Eucommia ulmoides group,)the Sargentodoxa group and the Phellinus igniarius group significantly decreased(P<0.05).Eucommia ulmoides and Caulis spatholobi are more effective in reducing pathological damage of toes in mice.Conclusion:The water extract of Caulis spatholobi has obvious anti-gout effect,and the most significant effect on analgesia and swelling.Eucommia ulmoides and Sargentodoxa cuneata have more obvious anti-inflammatory effects and have certain analgesic and swelling effects.Phellinus Phellinus igniarius has obvious inhibitory effect on IL-1β,but it does not show good anti-gout potential.展开更多
Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were c...Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were carefully selected based on the literature review and their traditional use.The antimicrobial activities of ethanol extracts of these medicinal plants were tested against fungi(Aspergillus funigaius),yeast(Candida albicans),gram-negative(Acirelobacter haumannii and Pseudornnruis aeruginosa)and gram-positive bacteria(Staphglococcus aureus).The activities were tested at three different concentrations of 1.00,0.10 and 0.01 mg/mL.The data was analysed using Gene data Screener program.Results:The measured antimicrobial activities indicated that out of the 58 plant extracts,15 extracts showed anti-fungal activity and 23 extracts exhibited anti-bacterial activity.Eight plant extracts have exhibited both anti-bacterial and anti-fungal activities.For instance,Eucommia ulmoides,Pohgonum cuspidcrtum,Poria cocas and Uncaria rhineophylla showed activity against both bacterial and fungal strains,indicating their broad spectrum of activity.Conclusions:The results revealed that the ethanol extracts of 30 plants out of the selected 58 possess significant antimicrobial activities.It is interesting to note that the findings from the current study are consistent with the traditional use.A clear correlation has also been found between the antimicrobial activity and the flavonoid content of the plant extracts which is in agreement with the literature.Hence.the results presented here can be used to guide the selection of potential plant species for the isolation and structure elucidation of novel antimicrobial compounds in order to establish the structure-activity relationship.This in turn is expected to lead the way to the discovery of novel antimicrobial agents for therapeutic use.展开更多
This paper was conducted to monitor the effect of mixed essential oils (AROMIX@) as phytogenic extract herbs administration in drinking water on growth performance, hematological, biochemical, immunological st...This paper was conducted to monitor the effect of mixed essential oils (AROMIX@) as phytogenic extract herbs administration in drinking water on growth performance, hematological, biochemical, immunological statuses and carcass traits of broiler chickens. A total number of 240 unsexed broiler chicks (Cobb-500) strain, were randomly divided to four equal groups, 60 chicks each at seven days of age. The first group drank tap water (control), the second, third and fourth group were drank the same water, adding 1 ml AROMIX@per 5, 10 and 15 liter tap water, respectively for 5 week of age. It was cleared from the results that there was a significant improve in feed conversion ratio, red and white blood cell counts, total protein, albumin, globulin, calcium, inorganic phosphorus and a relative reduction in the liver enzymes but in the normal range. However, it was observed that mix essential oils have no significant impact on carcass organs. High levels of the vital parameters, low levels of hepatic enzymes showed that birds health was good and relative improvement in immune system. The relative improvement of the absorption of minerals such as calcium and inorganic phosphorus to the positive role of essential oils. Administration in drank water was occasion, a regular activity of hepatic enzymes which can evidence safety and hepato-protective nature of these oils. Therefore, it is cleared from the results that the addition of these extract herbs in drinking water enhances the health meat, performance and physiological status of broiler chicks, which helps to obtain healthy meat and suitable for human consumption.展开更多
Angelica dahurica is commonly referred to as ‘Baizhi’ in China and has been noted for its therapeutic significance. The major active ingredients of Angelica dahurica is coumarin, which is reported as a kind of poten...Angelica dahurica is commonly referred to as ‘Baizhi’ in China and has been noted for its therapeutic significance. The major active ingredients of Angelica dahurica is coumarin, which is reported as a kind of potent inhibitor of cytochrome P450 enzymes (CYP450s). The aim of this study was to investigate the inhibition of CYP3A enzymes by total coumarin extract (TCE) obtained from dried root of Angelica dahurica by using in situ single pass intestinal perfusion (SPIP) and in situ liver perfusion in rats. When midazolam (MDZ) which is a substrate of CYP3A co-perfused with TCE (198 μg/mL) from Baizhi in duodenum and ileum segments, the Peff of MDZ has increased significantly compared with the MDZ single perfused group (p 0.05) (n = 6). During in situ liver perfusion study, the results demonstrated that, 3 days oral administration of TCE obtained from Baizhi could significantly reduce the elimination rate of MDZ in the perfusate (p Angelica dahurica extract co-administrated with drugs which are the substrates of CYP3A, much more attention should be paid rather than that of other CYP450 enzymes. These findings may facilitate in predicting possible herb-drug interactions (HDIs) when Angelica dahurica is used in combination with other drugs, and decrease the incidence of the CYP450-mediated HDIs.展开更多
The present study analyzed the effects of ethanol extracts of scorpion on epilepsy prevention and hippocampal expression of glial fibrillary acidic protein in a lithium chloride-pilocarpine epileptic rat model. Result...The present study analyzed the effects of ethanol extracts of scorpion on epilepsy prevention and hippocampal expression of glial fibrillary acidic protein in a lithium chloride-pilocarpine epileptic rat model. Results were subsequently compared with valproic acid. Results showed gradually- increased hippocampal glial fibrillary acidic protein expression following model establishment; glial fibrillary acidic protein mRNA expression was significantly increased at 3 days, reached a peak at 7 days, and then gradually decreased thereafter. Ethanol extracts of scorpion doses of 580 and 1 160 mg/kg, as well as 120 mg/kg valproic acid, led to a decreased number of glial fibrillary acidic protein-positive cells and glial fibrillary acidic protein mRNA expression, as well as decreased seizure grades and frequency of spontaneously recurrent seizures. The effects of 1 160 mg/kg ethanol extracts of scorpion were equal to those of 120 mg/kg valproic acid. These results suggested that the anti-epileptic effect of ethanol extracts of scorpion were associated with decreased hippocampal glial fibrillary acidic protein expression in a rat model of lithium chlofide-pilocarpine induced epilepsy.展开更多
文摘[ Objective] The paper presents the effects of Chinese herbal extract on the performance of local chickens for its application in animal production. [Method] The herbal extract Ⅰ and Ⅱ were added to the basal diet of 10 day-old chickens at the ratio of 0.6%, respectively. Then the chickens was fasted and respectively weighed at 0, 17, 24 and 31 days old. And the feed consumption and mortality of each group were recorded to determine average weight, average daily gain, and feed conversion ratio. Economic benefit was also analyzed. [ Result] There was no significant difference in average daily gain between the test groups and the control group as well as between the two test groups （P〉0.05） ; there was signifi- cant difference in feed conversion ratio between the test groups and the control group （ P 〈0.05）, but no significant difference between the two test groups （ P 〉 0.05） ; the survival rate and the economic benefits of the test groups were significantly higher than that of control （ P 〈 0.05）. [ Conclusion ] Chinese herbal extract Ⅰ and Ⅱ should improve chicken average daily gain, feed conversion ratio and survival rate, promote growth, and signifi- cantly improve economic benefit.
文摘The objective of this research was to investigate the effects of a natural herb extract on the general performance, carcass parameters and mortality of broiler chicks. A total of 720 one-day old chicks (Cobb 500) were used in the experiment and randomly partitioned into nine pens (80 chicks per pen). Three experimental diets (C: regular starter feed;CC plus: regular starter feed supplemented with chicken plus herbal extract;AFC plus: antibiotic-free diet supplemented with chicken plus) were assigned each to three randomly selected pens. The herbal extract was supplemented in drinking water at rate of 300 ml/cubic meter. Results showed that weights, feed conversion ratios (FCR) and dressing percentages (DP) increased (P < 0.05) in birds supplemented with herb extract compared to control birds. Mortalities and sudden deaths were minimized via herbal supplementation. However, herbal supplementation had no significant effects on carcass cuts and some visceral organs. The relative economic efficiency (REE) was up to 13% improved by the herb supplementation. It can be concluded that feeding the herbal extract has significant positive effects on broilers general performance as feed efficiency is increased by 11% and on mortality and sudden death cases.
文摘Recently reports showed that the pathobiology of acne vulgaris was arising from the exploration of sebaceous gland biology, hormonal factors, hyper-keratinization, and the role of bacteria, sebum, nutrition, cytokines and toll-like receptors (TLRs). Propionibacterium acnes (P.acnes) has a strong proinflammatory activity and targets molecules involved in the innate cutaneous immunity on keratinocytes by acting on TLR-2, leads to the development of comedones. GMP, a multi-herb extraction, targeted most of the major pathogenic features of acne with desired physicochemical traits. It strongly suppressed P.acnes growth, and reduced inflammation by suppressing the TLR-2/NF- kB pathway in SZ-95 sebocytes and HaCaT keratinocytes. GMP exhibited a marginal effect on cell viability and may have modulated hyper-keratinization of the epidermis. These results demonstrate the clinical feasibility of applying GMP for the treatment of acne.
基金National Natural Science Foundation of China(No.81960663)2016 Student Innovation Training Program of Hainan Medical University(No.HYCX2016055)+1 种基金2017 College Student Innovation and Entrepreneurship Training Program of Hainan Province(No.2017098)2018 Research Start Funding of Hainan Medical University。
文摘Objective:To explore the anti-gout effects of four traditional Chinese medicine extracts of Eucommia,Sargentodoxa cuneata,Caulis spatholobi and Phellinus igniarius.Methods:Monosodium Urate(MSU)was used to establish a mouse gouty arritis model.With coloricine as the positive control group,the effects of four traditional Chinese medicine extracts of Ecuador,Sargentodoxa,Caulis spatholobi and Phellinus igniarius on rice were studied;The hot plate method was used to observe the analytical effects of the four groups of traditional Chinese medicine extracts;the enzyme-linked immunoassay(ELISA)was used to detect residual interference-1 beta(IL-1 beta);the four traditional Chinese medicine extracts were simultaneously determined on the white blood cells,and monocytes in the blood of mice as well as the influence of neutrophils and histopathological changes of toes.Results:Compared with the normal group,the joint diameter ratio,blood leukocytes,monocytes,neutrophils,IL-1βat different time points in the model group were significantly increased(P<0.01),and the pain percentage was significantly reduced(P<0.05).Compared with the model group,the joint diameter ratio of the colchicine group,the Eucommia group,the Sargentodoxa group,the Caulis spatholobi group and the Phellinus sibiricum group was significantly reduced(P<0.05),and the pain percentage was significantly increased(P(0.05).The four groups of traditional Chinese medicine extracts significantly decreased the white blood cells,monocytes,and neutrophils in the blood of mice(P<0.05),and the IL-1βexpression levels of the Eucommia ulmoides group,)the Sargentodoxa group and the Phellinus igniarius group significantly decreased(P<0.05).Eucommia ulmoides and Caulis spatholobi are more effective in reducing pathological damage of toes in mice.Conclusion:The water extract of Caulis spatholobi has obvious anti-gout effect,and the most significant effect on analgesia and swelling.Eucommia ulmoides and Sargentodoxa cuneata have more obvious anti-inflammatory effects and have certain analgesic and swelling effects.Phellinus Phellinus igniarius has obvious inhibitory effect on IL-1β,but it does not show good anti-gout potential.
基金funded by the University of Western Sydney and the Fundacion MEDINAa public-private partnership of Merck Sharp&Dohme de Espana S.A./Universidad de Granada/Junta de Andalucia
文摘Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were carefully selected based on the literature review and their traditional use.The antimicrobial activities of ethanol extracts of these medicinal plants were tested against fungi(Aspergillus funigaius),yeast(Candida albicans),gram-negative(Acirelobacter haumannii and Pseudornnruis aeruginosa)and gram-positive bacteria(Staphglococcus aureus).The activities were tested at three different concentrations of 1.00,0.10 and 0.01 mg/mL.The data was analysed using Gene data Screener program.Results:The measured antimicrobial activities indicated that out of the 58 plant extracts,15 extracts showed anti-fungal activity and 23 extracts exhibited anti-bacterial activity.Eight plant extracts have exhibited both anti-bacterial and anti-fungal activities.For instance,Eucommia ulmoides,Pohgonum cuspidcrtum,Poria cocas and Uncaria rhineophylla showed activity against both bacterial and fungal strains,indicating their broad spectrum of activity.Conclusions:The results revealed that the ethanol extracts of 30 plants out of the selected 58 possess significant antimicrobial activities.It is interesting to note that the findings from the current study are consistent with the traditional use.A clear correlation has also been found between the antimicrobial activity and the flavonoid content of the plant extracts which is in agreement with the literature.Hence.the results presented here can be used to guide the selection of potential plant species for the isolation and structure elucidation of novel antimicrobial compounds in order to establish the structure-activity relationship.This in turn is expected to lead the way to the discovery of novel antimicrobial agents for therapeutic use.
文摘This paper was conducted to monitor the effect of mixed essential oils (AROMIX@) as phytogenic extract herbs administration in drinking water on growth performance, hematological, biochemical, immunological statuses and carcass traits of broiler chickens. A total number of 240 unsexed broiler chicks (Cobb-500) strain, were randomly divided to four equal groups, 60 chicks each at seven days of age. The first group drank tap water (control), the second, third and fourth group were drank the same water, adding 1 ml AROMIX@per 5, 10 and 15 liter tap water, respectively for 5 week of age. It was cleared from the results that there was a significant improve in feed conversion ratio, red and white blood cell counts, total protein, albumin, globulin, calcium, inorganic phosphorus and a relative reduction in the liver enzymes but in the normal range. However, it was observed that mix essential oils have no significant impact on carcass organs. High levels of the vital parameters, low levels of hepatic enzymes showed that birds health was good and relative improvement in immune system. The relative improvement of the absorption of minerals such as calcium and inorganic phosphorus to the positive role of essential oils. Administration in drank water was occasion, a regular activity of hepatic enzymes which can evidence safety and hepato-protective nature of these oils. Therefore, it is cleared from the results that the addition of these extract herbs in drinking water enhances the health meat, performance and physiological status of broiler chicks, which helps to obtain healthy meat and suitable for human consumption.
文摘Angelica dahurica is commonly referred to as ‘Baizhi’ in China and has been noted for its therapeutic significance. The major active ingredients of Angelica dahurica is coumarin, which is reported as a kind of potent inhibitor of cytochrome P450 enzymes (CYP450s). The aim of this study was to investigate the inhibition of CYP3A enzymes by total coumarin extract (TCE) obtained from dried root of Angelica dahurica by using in situ single pass intestinal perfusion (SPIP) and in situ liver perfusion in rats. When midazolam (MDZ) which is a substrate of CYP3A co-perfused with TCE (198 μg/mL) from Baizhi in duodenum and ileum segments, the Peff of MDZ has increased significantly compared with the MDZ single perfused group (p 0.05) (n = 6). During in situ liver perfusion study, the results demonstrated that, 3 days oral administration of TCE obtained from Baizhi could significantly reduce the elimination rate of MDZ in the perfusate (p Angelica dahurica extract co-administrated with drugs which are the substrates of CYP3A, much more attention should be paid rather than that of other CYP450 enzymes. These findings may facilitate in predicting possible herb-drug interactions (HDIs) when Angelica dahurica is used in combination with other drugs, and decrease the incidence of the CYP450-mediated HDIs.
基金sponsored by the National Natural Science Foundation of China, No. 30740035Key Scientific and Technological Project of Sichuan Province, No. 05SG1672
文摘The present study analyzed the effects of ethanol extracts of scorpion on epilepsy prevention and hippocampal expression of glial fibrillary acidic protein in a lithium chloride-pilocarpine epileptic rat model. Results were subsequently compared with valproic acid. Results showed gradually- increased hippocampal glial fibrillary acidic protein expression following model establishment; glial fibrillary acidic protein mRNA expression was significantly increased at 3 days, reached a peak at 7 days, and then gradually decreased thereafter. Ethanol extracts of scorpion doses of 580 and 1 160 mg/kg, as well as 120 mg/kg valproic acid, led to a decreased number of glial fibrillary acidic protein-positive cells and glial fibrillary acidic protein mRNA expression, as well as decreased seizure grades and frequency of spontaneously recurrent seizures. The effects of 1 160 mg/kg ethanol extracts of scorpion were equal to those of 120 mg/kg valproic acid. These results suggested that the anti-epileptic effect of ethanol extracts of scorpion were associated with decreased hippocampal glial fibrillary acidic protein expression in a rat model of lithium chlofide-pilocarpine induced epilepsy.
