A rapid headspace single-drop micro-extraction（mix） gas chromatography mass spectrometry（SDMEGC -MS） for the analysis of the volatile compounds in Herba Asari was developed in this study.A mixed solvent of n-tride...A rapid headspace single-drop micro-extraction（mix） gas chromatography mass spectrometry（SDMEGC -MS） for the analysis of the volatile compounds in Herba Asari was developed in this study.A mixed solvent of n-tridecane and butyl acetate（1：1） was finally used for the extraction at 70 C for 15 min with sample amount of 0.750 g and 100 mesh particle size.Under the determined conditions,the pound samples of Herba Asari were directly applied for the analysis.SDME-GC-MS,SPME-GC-MS and SD-GCMS methods were compared and the results showed that SDME-GC-MS method was a simple, inexpensive and effective way to measure the volatile compounds in Herba Asari and could be used for the analysis of volatile compounds in complex samples.展开更多
OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induc...OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induced by arthrectomy and assigned to sham-operated, model, EM, or EM plus HAM groups.All rats were injected with recombinant human granulocyte colony-stimulating factor 30μg·kg-1·d-1for7 days and treated with EMor EM plus HAM at 1.6 or 1.9 g·kg-1·d-1 for 3 or 6 weeks, respectively. Chondrocyte apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining. Levels of interleukin-1 beta(IL-1β) tumor necrosis factor alpha(TNF-α) nitric oxide(NO), and inducible nitric oxide synthase(iNOS) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay. Matrix metalloproteinases(MMPs)-13,tissue inhibitors of metalloproteinases(TIMPs)-1,Bromodeoxyuridine(BrdU), cluster of differentiation 34(CD34), and stromal cell-derived factor 1(SDF-1) were measured by immunohistochemical assay.RESULTS:The EM and EM plus HAM groups had significantly less cartilage damage and synovium inflammation the model group. Moreover, the EM and EM plus HAM groups had less chondrocyte apoptosis and more proteoglycan and collagen content than the model group.The EM and EMplus HAM groups had obviously higher MMPs-13 and TIMPs-1 expression in the cartilage than the model group. Moreover, the two formula groups had less release of IL-1β, TNF-α, NO, and iNOS than model group. Importantly, the expressions of BrdU, CD34,and SDF-1 in cartilage were significantly higher in the EM and EM plus HAM-Medtreated rats than model group. Notably, the EM plus HAM treatment seemed to have the greatest effects.CONCLUSION: HAM improves the therapeutic effects of EM on OA rats by enhancing BMSC directional homing to the focal zone.展开更多
目的:对细辛类植物中马兜铃酸 A 进行定性及定量分析。方法:采用 HPLC 法测定18份细辛属药材、7个马兜铃属药材中马兜铃酸 A 的含量。色谱柱为 HiQ-Sil C_(18)(250mm×4.6mm,5μm),流动相为甲醇-水-醋酸(60:36:4),流速为1.0mL·...目的:对细辛类植物中马兜铃酸 A 进行定性及定量分析。方法:采用 HPLC 法测定18份细辛属药材、7个马兜铃属药材中马兜铃酸 A 的含量。色谱柱为 HiQ-Sil C_(18)(250mm×4.6mm,5μm),流动相为甲醇-水-醋酸(60:36:4),流速为1.0mL·min^(-1),柱温20℃,检测波长316nm。同时采用 LC/MS 法对细辛中马兜铃酸 A 结构进行确认。结果:HPLC 法测定马兜铃酸 A 在0.01～0.25μg范围内具良好的线性关系(r=0.9999),平均回收率为97.5%,RSD 为1.8%。HPLC 色谱图中保留时间为9.036min 的色谱峰,经 LC/MS 确证为马兜铃酸 A。结论:HPLC 法可用于细辛类植物中马兜铃酸 A 的定性及定量测定。展开更多
目的:通过检测细辛、藜芦配伍使用前后所含化学成分的变化,研究传统中药理论"十八反"的科学内涵。方法:利用UPLC/Q-TOF MS BPI离子流图谱,分别绘制细辛单煎液、藜芦单煎液和细辛与藜芦配伍混煎液的BPI离子流指纹图谱,对图谱...目的:通过检测细辛、藜芦配伍使用前后所含化学成分的变化,研究传统中药理论"十八反"的科学内涵。方法:利用UPLC/Q-TOF MS BPI离子流图谱,分别绘制细辛单煎液、藜芦单煎液和细辛与藜芦配伍混煎液的BPI离子流指纹图谱,对图谱中分子离子峰进行分析,通过loadings确定差异分子离子,比较相同分子离子峰单煎、配伍混煎前后量的变化。结果:分别确定配伍混煎后细辛中有八个分子离子、藜芦中有十个分子离子峰峰面积变化超过9%以上。结论:从药理作用的物质基础研究,细辛"反"藜芦是具有一定道理的。展开更多
文摘A rapid headspace single-drop micro-extraction（mix） gas chromatography mass spectrometry（SDMEGC -MS） for the analysis of the volatile compounds in Herba Asari was developed in this study.A mixed solvent of n-tridecane and butyl acetate（1：1） was finally used for the extraction at 70 C for 15 min with sample amount of 0.750 g and 100 mesh particle size.Under the determined conditions,the pound samples of Herba Asari were directly applied for the analysis.SDME-GC-MS,SPME-GC-MS and SD-GCMS methods were compared and the results showed that SDME-GC-MS method was a simple, inexpensive and effective way to measure the volatile compounds in Herba Asari and could be used for the analysis of volatile compounds in complex samples.
基金Supported by Grants from the National Natural Science Foundation of China Project of Guiding Traditional Chinese Medicine Induced Bone Marrow Stem Cell Directional Homing to a Focal Zone for the Treatment of Osteoarthritis(No.81072900)
文摘OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induced by arthrectomy and assigned to sham-operated, model, EM, or EM plus HAM groups.All rats were injected with recombinant human granulocyte colony-stimulating factor 30μg·kg-1·d-1for7 days and treated with EMor EM plus HAM at 1.6 or 1.9 g·kg-1·d-1 for 3 or 6 weeks, respectively. Chondrocyte apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining. Levels of interleukin-1 beta(IL-1β) tumor necrosis factor alpha(TNF-α) nitric oxide(NO), and inducible nitric oxide synthase(iNOS) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay. Matrix metalloproteinases(MMPs)-13,tissue inhibitors of metalloproteinases(TIMPs)-1,Bromodeoxyuridine(BrdU), cluster of differentiation 34(CD34), and stromal cell-derived factor 1(SDF-1) were measured by immunohistochemical assay.RESULTS:The EM and EM plus HAM groups had significantly less cartilage damage and synovium inflammation the model group. Moreover, the EM and EM plus HAM groups had less chondrocyte apoptosis and more proteoglycan and collagen content than the model group.The EM and EMplus HAM groups had obviously higher MMPs-13 and TIMPs-1 expression in the cartilage than the model group. Moreover, the two formula groups had less release of IL-1β, TNF-α, NO, and iNOS than model group. Importantly, the expressions of BrdU, CD34,and SDF-1 in cartilage were significantly higher in the EM and EM plus HAM-Medtreated rats than model group. Notably, the EM plus HAM treatment seemed to have the greatest effects.CONCLUSION: HAM improves the therapeutic effects of EM on OA rats by enhancing BMSC directional homing to the focal zone.
文摘目的:对细辛类植物中马兜铃酸 A 进行定性及定量分析。方法:采用 HPLC 法测定18份细辛属药材、7个马兜铃属药材中马兜铃酸 A 的含量。色谱柱为 HiQ-Sil C_(18)(250mm×4.6mm,5μm),流动相为甲醇-水-醋酸(60:36:4),流速为1.0mL·min^(-1),柱温20℃,检测波长316nm。同时采用 LC/MS 法对细辛中马兜铃酸 A 结构进行确认。结果:HPLC 法测定马兜铃酸 A 在0.01～0.25μg范围内具良好的线性关系(r=0.9999),平均回收率为97.5%,RSD 为1.8%。HPLC 色谱图中保留时间为9.036min 的色谱峰,经 LC/MS 确证为马兜铃酸 A。结论:HPLC 法可用于细辛类植物中马兜铃酸 A 的定性及定量测定。
文摘目的:通过检测细辛、藜芦配伍使用前后所含化学成分的变化,研究传统中药理论"十八反"的科学内涵。方法:利用UPLC/Q-TOF MS BPI离子流图谱,分别绘制细辛单煎液、藜芦单煎液和细辛与藜芦配伍混煎液的BPI离子流指纹图谱,对图谱中分子离子峰进行分析,通过loadings确定差异分子离子,比较相同分子离子峰单煎、配伍混煎前后量的变化。结果:分别确定配伍混煎后细辛中有八个分子离子、藜芦中有十个分子离子峰峰面积变化超过9%以上。结论:从药理作用的物质基础研究,细辛"反"藜芦是具有一定道理的。
