In urban road greening,the planting of ground cover flowers is essential,and herbaceous flowers are an important part.Through the investigation on the diversity of herbaceous flowers in urban road green spaces of Hefe...In urban road greening,the planting of ground cover flowers is essential,and herbaceous flowers are an important part.Through the investigation on the diversity of herbaceous flowers in urban road green spaces of Hefei City,the types,colors,application frequency and planting of herbaceous flowers in urban road green spaces were statistically analyzed,and the application forms of herbaceous flowers in the road greening of Hefei City were studied.The characteristics and problems of herbaceous flowers in the road greening of Hefei City were found,and some development suggestions were put forward.展开更多
Herbaceous peony(Paeonia lactiflora Pall.) is a famous flower with medicinal values, and its flowers have a number of medicinal constituents, especially flavonoids. In this study, a P. lactiflora cultivar with doubl...Herbaceous peony(Paeonia lactiflora Pall.) is a famous flower with medicinal values, and its flowers have a number of medicinal constituents, especially flavonoids. In this study, a P. lactiflora cultivar with double colors including white outer-petal and yellow inner-petal was used as the experimental materials to perform the qualitative and quantitative analysis of flavonoids by high-performance liquid chromatograph-electrospray ionization-mass spectrometry(HPLC-ESI-MSn) and investigate the expression patterns of flavonoid biosynthetic genes using real-time quantitative polymerase chain reaction(Q-PCR). The results showed that the colors of both petals gradually weakened with flower development. Moreover, one main anthocyanin composition(peonidin 3,5-di-O-glucoside) and five main anthoxanthin compositions(kaempferol di-hexoside, kaempferol-3-O-malonylglucoside-7-O-glucoside, quercetin-3-O-galactoside, luteolin-7-O-glucoside and isorhamnetin-3-O-glucoside) were found in the both, differing significantly in their peak areas only. Total anthocyanin, anthoxanthin and flavonoid contents in white outer-petal and yellow inner-petal gradually decreased during flower development, and were consistently higher in white outer-petal. Furthermore, the expression patterns of nine structural genes in P. lactiflora flavonoid biosynthetic pathway showed that the expression levels of phenylalanine ammonialyase gene(Pl PAL), chalcone synthase gene(PlC HS), flavanone 3-hydroxylase gene(PlF 3H), anthocyanidin synthase gene(PlA NS) and UDP-glucoside: flavonoid 5-O-glucosyltransferase gene(Pl F5GT) in two petals basically presented declined tendencies, and transcription levels of Pl PAL, Pl CHS, Pl ANS, Pl F3 GT and Pl F5 GT also tended to be higher in white outer-petal, which was correlated with their flavonoid contents. These results would lay a solid foundation for the exploration and utilization of flavonoid resources in P. lactiflora flowers.展开更多
基金Sponsored by the Innovation Training Project for College Students in Anhui Province(S202212216130)Key Research Project of Natural Science in Universities of Anhui Province(2023AH051816)General Teaching Research Project of Anhui Province(2022jyxm665).
文摘In urban road greening,the planting of ground cover flowers is essential,and herbaceous flowers are an important part.Through the investigation on the diversity of herbaceous flowers in urban road green spaces of Hefei City,the types,colors,application frequency and planting of herbaceous flowers in urban road green spaces were statistically analyzed,and the application forms of herbaceous flowers in the road greening of Hefei City were studied.The characteristics and problems of herbaceous flowers in the road greening of Hefei City were found,and some development suggestions were put forward.
基金supported by the National Natural Science Foundation of China(31372097 and 31400592)the Major Project of College Natural Science Research of Jiangsu Province,China(13KJA210005)+1 种基金the Opening Project of Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,China(2014014)the Priority Academic Program Development from Jiangsu Government,China
文摘Herbaceous peony(Paeonia lactiflora Pall.) is a famous flower with medicinal values, and its flowers have a number of medicinal constituents, especially flavonoids. In this study, a P. lactiflora cultivar with double colors including white outer-petal and yellow inner-petal was used as the experimental materials to perform the qualitative and quantitative analysis of flavonoids by high-performance liquid chromatograph-electrospray ionization-mass spectrometry(HPLC-ESI-MSn) and investigate the expression patterns of flavonoid biosynthetic genes using real-time quantitative polymerase chain reaction(Q-PCR). The results showed that the colors of both petals gradually weakened with flower development. Moreover, one main anthocyanin composition(peonidin 3,5-di-O-glucoside) and five main anthoxanthin compositions(kaempferol di-hexoside, kaempferol-3-O-malonylglucoside-7-O-glucoside, quercetin-3-O-galactoside, luteolin-7-O-glucoside and isorhamnetin-3-O-glucoside) were found in the both, differing significantly in their peak areas only. Total anthocyanin, anthoxanthin and flavonoid contents in white outer-petal and yellow inner-petal gradually decreased during flower development, and were consistently higher in white outer-petal. Furthermore, the expression patterns of nine structural genes in P. lactiflora flavonoid biosynthetic pathway showed that the expression levels of phenylalanine ammonialyase gene(Pl PAL), chalcone synthase gene(PlC HS), flavanone 3-hydroxylase gene(PlF 3H), anthocyanidin synthase gene(PlA NS) and UDP-glucoside: flavonoid 5-O-glucosyltransferase gene(Pl F5GT) in two petals basically presented declined tendencies, and transcription levels of Pl PAL, Pl CHS, Pl ANS, Pl F3 GT and Pl F5 GT also tended to be higher in white outer-petal, which was correlated with their flavonoid contents. These results would lay a solid foundation for the exploration and utilization of flavonoid resources in P. lactiflora flowers.