Comparative analysis of volatile constituents between herbal pair flos lonicerae-caulis lonicerae and its single herbs

Gas chromatography-mass spectrometry(GC-MS) and the chemometric resolution method(alternative moving window factor analysis,AMWFA) were used for comparative analysis of volatile constituents in herbal pair(HP) flos lonicerae-caulis lonicerae(FL-CL) and its single herbs.The temperature-programmed retention index(PTRI) was also employed for the identification of compounds.In total,44,39,and 50 volatile chemical components in volatile oil of FL,CL and HP FL-CL were separately determined qualitatively and quantitatively,accounting for 87.22%,94.54% and 90.08% total contents of volatile oil of FL,CL and HP FL-CL,respectively.The results show that there are 32 common volatile constituents between HP FL-CL and single herb FL,33 common volatile constituents between HP FL-CL and single herb CL,and 10 new constituents in the volatile oil of HP FL-CL.

基于GC-MS结合HELP法的药对金银花—连翘及其单味药挥发性化学成分分析

采用气相色谱—质谱(GC-MS)技术和化学计量学新方法——直观推导式演进特征投影法分析药对金银花—连翘及单味药的挥发性化学成分。从药对金银花—连翘及其单味药金银花、连翘的挥发油中分别鉴定了46、12和28种化合物,分别占总挥发油相对含量的96.84%、91.51%和96.94%。三者共有挥发油成分5种。从化合物数目上看,药对金银花—连翘的挥发性化学成分主要来自连翘,但各物质的含量发生了改变。结果表明,中药配伍对挥发性化学成分及含量均存在一定影响。

药对金银花-金银藤与其单味药挥发油组分的比较研究

目的定性、定量比较药对金银花-金银藤与其单味药的挥发油组分。方法利用气相色谱-质谱法测定挥发油成分,再用交互移动窗口因子分析法进行比较分析。程序升温保留指数也被采用以辅助鉴定化合物。结果金银花、金银藤和药对金银花-金银藤的挥发油分别定性了44,39和50个成分,定性组分含量分别占金银花、金银藤和药对金银花-金银藤挥发油总含量的87.22%,94.54%和90.08%。结论药对金银花-金银藤与单味药金银花的共有挥发油组分为32个,与单味药金银藤共有的挥发油组分为33个,三者共有的挥发油组分为25个,药对挥发油出现了10个新的化学成分。