Chemical component analysis of volatile oil in drug pair Herba Ephedrae-Ramulus Cinnamomi by GC-MS and CRM

Active volatile components in drug pair(DP)Herba Ephedrae-Ramulus Cinnamomi(HE-RC),single drug HE and RC were analyzed by gas chromatography/mass spectrometry(GC/MS),chemometric resolution method(CRM)and overall volume integration.By means of CRM,the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound.In total,97,62,and 78 volatile chemical components in volatile oil of HE,RC,and DP HE-RC,were respectively determined qualitatively and quantitatively,accounting for 90.08%,91.62%,and 89.76% total contents of volatile oil of HE,RC,and DP HE-RC respectively.It is further demonstrated that the numbers of volatile components of DP HE-RC are almost the sum of those of two single drugs,but some relative contents of them are changed.Some new components,such as 1,6-dimethylhepta-1,3,5-triene,tetracyclo[4.2.1.1(2,5).0(9,10)]deca-3,7-diene,globulol and(E,E)-6,10,14-trimethyl-5,9,13-pentadecatrien-2-one are found in DP HE-RC because of chemical reactions and physical changes during decoction.

Analysis of volatile components in herbal pair herba schizonepetae-ramulus cinnamomi

Analysis of volatile components in herbal pair (HP) herba schizonepetae-ramulus cinnamomi (HS-RC), single herb HS and RC was carried out by gas chromatography-mass spectrometry (GC-MS) data and chemometric resolution method (CRM). The two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound by CRM. In total, 47, 61 and 51 chemical components in volatile oil of HS, RC, and HP HS-RC were respectively determined qualitatively and quantitatively, accounting for 90.52%, 88.37%, and 88.72% total contents of volatile oil of HS, RC, and HP HS-RC, respectively. The number of the volatile components of HP HS-RC is almost the addition of that of two single herbs, but their relative contents are changed.

气相色谱-质谱和化学计量学解析法分析药对麻黄-桂枝挥发油成分

利用气相色谱-质谱对药对麻黄-桂枝及单味药麻黄和桂枝的挥发油成分进行检测,通过化学计量学解析法对产生的二维色谱-质谱数据进行解析,得到各组分的纯色谱和质谱,根据其保留时间和质谱,在质谱库中进行相似检索,实现对组分的定性,再用总体积积分法进行定量。麻黄-桂枝、麻黄和桂枝挥发油分别定性了97,72和68个色谱峰,占总含量的89.76%,90.08%和91.62%。药对挥发油成分的数目大致为麻黄和桂枝挥发油成分的加和,但相对含量有变化。

药对荆芥-桂枝挥发油成分的气相色谱-质谱和化学计量学方法分析

目的分析药对荆芥-桂枝、单味药荆芥,桂枝的挥发油成分。方法采用气相色谱-质谱(GC-MS)检测,通过化学计量学解析法对二维色谱/质谱数据进行解析,从而实现对荆芥-桂枝、单味药荆芥、桂枝的挥发油成分的分析。结果荆芥-桂枝、荆芥和桂枝挥发油成分分别定性得到51,47和61个结果,占总含量的88.72%,90.52%和88.37%。结论药对挥发油成分的数目大致为荆芥和桂枝挥发油成分的加和,但相对含量有变化。

药对荆芥-防风挥发油成分的分析

目的定性、定量分析药对荆芥-防风、单味药荆芥和防风的挥发油成分。方法采用气相色谱-质谱(GC-MS)方法测定挥发油成分,利用化学计量学解析法分辨色谱重叠峰,通过质谱库进行定性,用总体积积分法进行定量。结果荆芥-防风、荆芥和防风挥发油分别得到65,51和36个定性结果,占总含量的82.36%,86.71%和89.15%。结论荆芥-防风挥发油成分数目大致是单味药荆芥和防风的加和,但主要来自荆芥。

药对青蒿-桂枝中挥发油成分的GC-MS分析与化学计量学解析

采用水蒸气蒸馏法从青蒿、桂枝及其药对中提取挥发油成分,利用气相色谱-质谱(GC-MS)联用技术进行分离检测,通过化学计量学解析法(CRM)对重叠色谱峰解析,并结合程序升温保留指数辅助定性。从药对及其单味药青蒿、桂枝中分别鉴定出69、66、68种挥发油;组成药对后,单味药成分减少,新成分增加,药对与单味药挥发油共存17种组分,它们是cis-细辛醚、丁子香烯、丁子香烯环氧化物等成分,占药对挥发油总含量的35.82%;而药对中挥发油的主要成分是(E)-桂皮醛(18.32%)和cis-细辛醚(10.20%)等,其含量并非单味药含量的简单相加,相关作用机理有待进一步研究。

正交试验结合熵权TOPSIS优选麻黄-桂枝药对水提工艺

目的:优选麻黄-桂枝药对的水提工艺。方法:建立麻黄-桂枝药对超高效液相色谱法指纹图谱,采用L9(34)正交试验设计,以煎煮次数、加水量、煎煮时间为考察因素,以指纹图谱及醇溶性浸出物的含量为评价指标,结合熵权逼近理想解排序法(TOPSIS)对正交水提工艺进行综合评价。结果:优选麻黄-桂枝药对最佳水提工艺为加10倍量水煎煮3次,每次煎煮30 min。结论:优选的麻黄-桂枝药对水提工艺科学合理、稳定可行,可为麻黄-桂枝药对的工业生产提供参考。

药对麻黄-葛根挥发油成分分析

目的定性定量分析药对麻黄-葛根、麻黄、葛根的挥发油成分。方法采用气相色谱-质谱(GC-MS)检测,通过化学计量学解析法对二维色谱/质谱数据进行解析。结果麻黄-葛根、麻黄和葛根挥发油成分分别定性得到57、70和33个结果,占总含量的93.17%、89.52%和88.84%。结论药对挥发油成分的数目大致为麻黄和葛根挥发油成分的加和。