Chemotherapy-induced toxicity(CIT)remains a major concern in cancer patients undergoing chemotherapy.New approaches to ameliorate the side effects of chemotherapy are urgently needed.Recently,the nutritional value of ...Chemotherapy-induced toxicity(CIT)remains a major concern in cancer patients undergoing chemotherapy.New approaches to ameliorate the side effects of chemotherapy are urgently needed.Recently,the nutritional value of citrus fruits has attracted wide attention.Hesperidin and its aglycone hesperetin are the main active components in citrus fruits.Hesperidin and hesperetin have a wide range of pharmacological activities,including antioxidant and anti-inflammatory properties.This review aims to provide insights into the potential application of citrus flavonoids in CIT and summarize the underlying mechanisms of hesperidin and hesperetin in alleviating CIT.We have collected and collated relevant scientific articles on hesperidin and hesperetin and their treatment of CIT from different scientific databases.Hesperidin and its glycosides can reduce the toxicity of chemotherapeutic drugs,and their therapeutic effects are mainly through anti-inflammatory and antioxidant effects.At present,modern medical treatment is the main treatment method for CIT,but hesperidin,as an extract of food and medicinal materials,can greatly alleviate CIT.While killing tumor cells,chemotherapeutic drugs also damage normal cells leading to toxic effect on various organs.The pathological mechanism of CIT has not been fully elucidated,but current evidences indicate that cellular stress plays a key role.The citrus flavonoids hesperidin and hesperetin have the protective effect against CIT,highlighting its potential as an adjuvant in chemotherapy regimens.Hesperidin may also have synergistic anti-tumor activity with chemotherapeutic agents.We believe that more functional foods and anti-CIT drugs based on natural foods will be developed.展开更多
Liver cancer is the fifth most common cancer and one of the leading causes of death in the world, and second most common cause of death in men. Natural products emerge as the most enduring approaches in the developmen...Liver cancer is the fifth most common cancer and one of the leading causes of death in the world, and second most common cause of death in men. Natural products emerge as the most enduring approaches in the development of anticancer targeting drug. Hesperetin(HP), one of the abundant flavonoids found naturally in citrus fruits, has received considerable attention in anti-cancer promotion and progression. The present study was conducted to decipher the role of 0.5 ml hesperetin conjugated gold nanoparticles(Au-m PEG(5000)-S-HP NPs) during diethylnitrosamine(DEN)-induced hepatocarcinogenesis in male Wistar albino rats and shows the better antioxidant that possesses anti-inflammatory,anti-proliferation and anticarcinogenic properties and may modulate signaling pathways.The confirmation of polymer functionalized gold nanoparticles and drug loaded polymer gold nanoparticles were characterized by HR-TEM with EDAX, and DLS with Zeta potential techniques. The drug encapsulation efficiency and release properties were carried out in PBS at pH 7.4 for Au-mPEG(5000)-S-HP and compared with the control pure hesperetin(HP).Here, we review the role of mast cell counts, tumor necrosis factor alpha(TNF-α), transcription factor nuclear factor-κB(NF-κB), levels of glycoconjugates, proliferating cell nuclear antigen(PCNA) and argyrophilic nucleolar organizing regions, are the master regulator of inflammation and proliferation, in the development of hepatocellular injury, liver fibrosis and HCC. DEN-administered animals showed increased mast cell counts, tumor necrosis factor alpha, transcription factor nuclear factor-κB, glycoconjugates, proliferating cell nuclear antigen, and argyrophilic nucleolar organizing regions. Whereas Au-mPEG(5000)-S-HP NPs supplementation considerably suppressed all the above abnormalities. These results suggest that the Au-m PEG(5000)-S-HP NPs exhibited the better potential anticancer activity by inhibiting cell inflammation and proliferation in DEN-induced hepatocellular carcinogenesis.展开更多
This study aimed to assess the effect of hesperetin and/or bone marrow-derived mesenchymal stem cells(BM-MSCs)on disturbed lipid profile,heart and kidney functions,oxidative stress and antioxidant defense system in st...This study aimed to assess the effect of hesperetin and/or bone marrow-derived mesenchymal stem cells(BM-MSCs)on disturbed lipid profile,heart and kidney functions,oxidative stress and antioxidant defense system in streptozotocin(STZ)-induced diabetic rats.Type 1 diabetes mellitus(T1DM)was induced in male Wistar rats by injecting 40 mg/kg body weight(b.w.)STZ dissolved in citrate buffer(pH 4.5).The diabetic rats were treated with hesperetin orally administered at dose 20 mg/kg b.w.,BM-MSCs intravenously injected at a dose of 1 x 106 cells/rat/week and their combination for 6 weeks.The diabetic rats exhibited lipid abnormalities manifested by elevated serum levels of total cholesterol,triglycerides,LDL-cholesterol and VLDL-cholesterol and lowered HDL-cholesterol as well as elevated liver cholesterol and triglycerides content in association with the resultant fasting and postprandial hyperglycemia and insulin deficiency.The heart function biomarkers including CK-MB,AST and LDH activities as well as levels of kidney function parameters,creatinine,and urea,were significantly raised in the serum of diabetic rats.These changes were concomitant with abnormal redox balance represented by elevated lipid peroxidation,decreased glutathione content,and suppressed antioxidant enzyme activities in both heart and kidney of diabetic rats.The previous deleterious alterations were significantly ameliorated after the treatment of diabetic rats with hesperetin and BM-MSCs singly or in combination;the treatment with hesperetin together with BM-MSCs was the most potent.Based on these findings,it can be concluded that the use of hesperetin with BM-MSCs may have more additive therapeutic value than their uses singly in T1DM.In addition,the ameliorative effects of hesperetin and BM-MSCs on lipid profile and heart and kidney functions in diabetic rats may be mediated,at least in part,via their suppressive effects on oxidative stress and ameliorative effects on the antioxidant defense system secondary to improvement in the hyperglycemia and insulin secretory response.展开更多
基于Hill动力学与Michaelis-Menten方程,建立理论模型研究发状分裂相关增强子1(Hairy and enhancer of split 1,Hes1)调控蛋白激酶B(Protein Kinase B,AKT)-鼠双微体2(Murine Double Minute2,MDM2)-抗癌基因p53(p53)-第10号染色体缺失...基于Hill动力学与Michaelis-Menten方程,建立理论模型研究发状分裂相关增强子1(Hairy and enhancer of split 1,Hes1)调控蛋白激酶B(Protein Kinase B,AKT)-鼠双微体2(Murine Double Minute2,MDM2)-抗癌基因p53(p53)-第10号染色体缺失的磷酸酶及张力蛋白同源的基因(Phosphatase and tensin homolog deleted on chromosome ten,PTEN)通路的一种物理机制.研究发现,Hes1通过与PTEN结合抑制PTEN表达,并调控AKT信号.表明了Hes1蛋白的合成,以及Hes1与PTEN相互作用调控AKT-MDM2-p53-PTEN通路信号,将会有效地控制细胞结果.Hes1作为AKT-MDM2-p53-PTEN信号通路中上游调节的重要因素,还可以在一定程度上通过影响p53蛋白功能,改变p53对肿瘤的抑制性.理论结果可用于预测Notch通路信号异常诱导的致癌性,并进一步揭示了Notch信号通路影响细胞AKT-MDM2-p53-PTEN通路的激活机制.展开更多
OBJECTIVE To investigate the neuroprotective effects of hesperetin on central neurons under chronic high glucose,and the relationship to glyoxalase 1(Glo-1),a cytoprotective enzyme.METHODS The human neuroblas⁃toma SH-...OBJECTIVE To investigate the neuroprotective effects of hesperetin on central neurons under chronic high glucose,and the relationship to glyoxalase 1(Glo-1),a cytoprotective enzyme.METHODS The human neuroblas⁃toma SH-SY5Y cells were divided into 5 groups:normal glucose,high glucose(HG),HG plus low,middle,or high concentra⁃tion of hesperetin(1,5,25μmol·L^-1).After treatment for 72 h,neuron damages,Glo-1 expressions and functions,as well as Nrf2/ARE pathway and its regulating mechanisms were examined.RESULTS Hesperetin increased cell viability and decreased lactate dehydrogenase release,which was accompanied by the elevated activity,protein,and mRNA levels of Glo-1 as well as the enhanced Glo-1 functions in SH-SY5Y cells cultured with HG.Moreover,hesperetin activated Nrf2/ARE pathway as evidenced by the raised Nrf2 and p-Nrf2 levels in nucleus and up-regulation of γ-glutamycysteine synthase(γ-GCS),a well-known target gene of Nrf2/ARE pathway.Nevertheless,pretreatment with a PKC inhibitor(Go 6983)or an Akt inhibitor(MK-22062HCl,reflecting GSK-3β activation)abolished the effect of hesperetin on protein expressions of Glo-1 and γ-GCS.CONCLUSION Hesperetin exerted the neuroprotection by promoting Glo-1 function in central neurons in long-term HG condition,which was mediated by activation of Nrf2/ARE pathway;moreover,the increased Nrf2 phosphorylation and nuclear translocation mediated by PKC activation and/or GSK-3β inhibition were involved in the activation of Nrf2/ARE pathway by hesperetin.展开更多
Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial...Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.展开更多
Today, consumers and producers makes more and more requests for determining the type of honey and its variety because its technological and medicinal qualities depends on the origin. There are many factors which affec...Today, consumers and producers makes more and more requests for determining the type of honey and its variety because its technological and medicinal qualities depends on the origin. There are many factors which affect on the quality of honey, but the most important is geographical and botanical origin of which largely depends on its medicinal properties. There are several methods of dealing with the classification of honey but the most accurate is pollen analysis with some other tests, such as pH, electrical conductivity and sensory properties. Flavonoids--as one of the most important group of plant secondary metabolites, can be found in honey and its number and amount directly dependents of the origin of honey. In this study, 48 honey samples were tested, of which 29 were pollen honey, 11 were mixed and 8 were honeydew. In all samples, regardless of the type of honey, quercetin and naringenin are found, and rutin and hesperetin are found in-between 36%-50% of honey samples. The largest individual and avarages amounts of tested flavonoids are found in the pollen honey and at least in the mixed honeys.展开更多
目的观察丹龙醒脑方对脑缺血再灌注模型大鼠侧脑室室管膜下区(SVZ)神经干细胞(NSCs)增殖与Hes1、Hes5表达的影响,探讨其促进内源性NSCs增殖的作用机制。方法将80只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组(依达组)、丹龙醒脑方...目的观察丹龙醒脑方对脑缺血再灌注模型大鼠侧脑室室管膜下区(SVZ)神经干细胞(NSCs)增殖与Hes1、Hes5表达的影响,探讨其促进内源性NSCs增殖的作用机制。方法将80只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组(依达组)、丹龙醒脑方组(丹龙组)。采用线栓法制备局灶性脑缺血再灌注模型,再灌注7 d后取缺血侧SVZ脑组织。Brdu免疫荧光法检测SVZ NSCs增殖,RT-q PCR、Western blot分别检测Hes1、Hes5 m RNA和蛋白的表达。结果与假手术组比较,其余各组Brdu阳性细胞率增加,Hes1、Hes5 m RNA及蛋白表达明显升高(P<0.01);与模型组比较,依达组、丹龙组Brdu阳性细胞率明显增加,Hes1、Hes5 m RNA及蛋白表达水平明显增强(P<0.01);丹龙组Hes1 m RNA表达水平优于依达组(P<0.01),其余指标均无明显差异。结论丹龙醒脑方可促进脑缺血再灌注后大鼠SVZ NSCs增殖,并上调Hes1、Hes5表达水平,其机制可能与激活Notch信号通路有关。展开更多
基金Innovative Team Project of Ordinary Universities in Guangdong Province(No.2022KCXTD016).
文摘Chemotherapy-induced toxicity(CIT)remains a major concern in cancer patients undergoing chemotherapy.New approaches to ameliorate the side effects of chemotherapy are urgently needed.Recently,the nutritional value of citrus fruits has attracted wide attention.Hesperidin and its aglycone hesperetin are the main active components in citrus fruits.Hesperidin and hesperetin have a wide range of pharmacological activities,including antioxidant and anti-inflammatory properties.This review aims to provide insights into the potential application of citrus flavonoids in CIT and summarize the underlying mechanisms of hesperidin and hesperetin in alleviating CIT.We have collected and collated relevant scientific articles on hesperidin and hesperetin and their treatment of CIT from different scientific databases.Hesperidin and its glycosides can reduce the toxicity of chemotherapeutic drugs,and their therapeutic effects are mainly through anti-inflammatory and antioxidant effects.At present,modern medical treatment is the main treatment method for CIT,but hesperidin,as an extract of food and medicinal materials,can greatly alleviate CIT.While killing tumor cells,chemotherapeutic drugs also damage normal cells leading to toxic effect on various organs.The pathological mechanism of CIT has not been fully elucidated,but current evidences indicate that cellular stress plays a key role.The citrus flavonoids hesperidin and hesperetin have the protective effect against CIT,highlighting its potential as an adjuvant in chemotherapy regimens.Hesperidin may also have synergistic anti-tumor activity with chemotherapeutic agents.We believe that more functional foods and anti-CIT drugs based on natural foods will be developed.
基金Indian Council of Medical Research, New Delhi, India, for the financial support provided in the form of Senior Research Fellowship (SRF-3/2/2/156/2011/NCD-Ⅲ)
文摘Liver cancer is the fifth most common cancer and one of the leading causes of death in the world, and second most common cause of death in men. Natural products emerge as the most enduring approaches in the development of anticancer targeting drug. Hesperetin(HP), one of the abundant flavonoids found naturally in citrus fruits, has received considerable attention in anti-cancer promotion and progression. The present study was conducted to decipher the role of 0.5 ml hesperetin conjugated gold nanoparticles(Au-m PEG(5000)-S-HP NPs) during diethylnitrosamine(DEN)-induced hepatocarcinogenesis in male Wistar albino rats and shows the better antioxidant that possesses anti-inflammatory,anti-proliferation and anticarcinogenic properties and may modulate signaling pathways.The confirmation of polymer functionalized gold nanoparticles and drug loaded polymer gold nanoparticles were characterized by HR-TEM with EDAX, and DLS with Zeta potential techniques. The drug encapsulation efficiency and release properties were carried out in PBS at pH 7.4 for Au-mPEG(5000)-S-HP and compared with the control pure hesperetin(HP).Here, we review the role of mast cell counts, tumor necrosis factor alpha(TNF-α), transcription factor nuclear factor-κB(NF-κB), levels of glycoconjugates, proliferating cell nuclear antigen(PCNA) and argyrophilic nucleolar organizing regions, are the master regulator of inflammation and proliferation, in the development of hepatocellular injury, liver fibrosis and HCC. DEN-administered animals showed increased mast cell counts, tumor necrosis factor alpha, transcription factor nuclear factor-κB, glycoconjugates, proliferating cell nuclear antigen, and argyrophilic nucleolar organizing regions. Whereas Au-mPEG(5000)-S-HP NPs supplementation considerably suppressed all the above abnormalities. These results suggest that the Au-m PEG(5000)-S-HP NPs exhibited the better potential anticancer activity by inhibiting cell inflammation and proliferation in DEN-induced hepatocellular carcinogenesis.
文摘This study aimed to assess the effect of hesperetin and/or bone marrow-derived mesenchymal stem cells(BM-MSCs)on disturbed lipid profile,heart and kidney functions,oxidative stress and antioxidant defense system in streptozotocin(STZ)-induced diabetic rats.Type 1 diabetes mellitus(T1DM)was induced in male Wistar rats by injecting 40 mg/kg body weight(b.w.)STZ dissolved in citrate buffer(pH 4.5).The diabetic rats were treated with hesperetin orally administered at dose 20 mg/kg b.w.,BM-MSCs intravenously injected at a dose of 1 x 106 cells/rat/week and their combination for 6 weeks.The diabetic rats exhibited lipid abnormalities manifested by elevated serum levels of total cholesterol,triglycerides,LDL-cholesterol and VLDL-cholesterol and lowered HDL-cholesterol as well as elevated liver cholesterol and triglycerides content in association with the resultant fasting and postprandial hyperglycemia and insulin deficiency.The heart function biomarkers including CK-MB,AST and LDH activities as well as levels of kidney function parameters,creatinine,and urea,were significantly raised in the serum of diabetic rats.These changes were concomitant with abnormal redox balance represented by elevated lipid peroxidation,decreased glutathione content,and suppressed antioxidant enzyme activities in both heart and kidney of diabetic rats.The previous deleterious alterations were significantly ameliorated after the treatment of diabetic rats with hesperetin and BM-MSCs singly or in combination;the treatment with hesperetin together with BM-MSCs was the most potent.Based on these findings,it can be concluded that the use of hesperetin with BM-MSCs may have more additive therapeutic value than their uses singly in T1DM.In addition,the ameliorative effects of hesperetin and BM-MSCs on lipid profile and heart and kidney functions in diabetic rats may be mediated,at least in part,via their suppressive effects on oxidative stress and ameliorative effects on the antioxidant defense system secondary to improvement in the hyperglycemia and insulin secretory response.
文摘基于Hill动力学与Michaelis-Menten方程,建立理论模型研究发状分裂相关增强子1(Hairy and enhancer of split 1,Hes1)调控蛋白激酶B(Protein Kinase B,AKT)-鼠双微体2(Murine Double Minute2,MDM2)-抗癌基因p53(p53)-第10号染色体缺失的磷酸酶及张力蛋白同源的基因(Phosphatase and tensin homolog deleted on chromosome ten,PTEN)通路的一种物理机制.研究发现,Hes1通过与PTEN结合抑制PTEN表达,并调控AKT信号.表明了Hes1蛋白的合成,以及Hes1与PTEN相互作用调控AKT-MDM2-p53-PTEN通路信号,将会有效地控制细胞结果.Hes1作为AKT-MDM2-p53-PTEN信号通路中上游调节的重要因素,还可以在一定程度上通过影响p53蛋白功能,改变p53对肿瘤的抑制性.理论结果可用于预测Notch通路信号异常诱导的致癌性,并进一步揭示了Notch信号通路影响细胞AKT-MDM2-p53-PTEN通路的激活机制.
基金National Natural Science Foundation of China(81371210)
文摘OBJECTIVE To investigate the neuroprotective effects of hesperetin on central neurons under chronic high glucose,and the relationship to glyoxalase 1(Glo-1),a cytoprotective enzyme.METHODS The human neuroblas⁃toma SH-SY5Y cells were divided into 5 groups:normal glucose,high glucose(HG),HG plus low,middle,or high concentra⁃tion of hesperetin(1,5,25μmol·L^-1).After treatment for 72 h,neuron damages,Glo-1 expressions and functions,as well as Nrf2/ARE pathway and its regulating mechanisms were examined.RESULTS Hesperetin increased cell viability and decreased lactate dehydrogenase release,which was accompanied by the elevated activity,protein,and mRNA levels of Glo-1 as well as the enhanced Glo-1 functions in SH-SY5Y cells cultured with HG.Moreover,hesperetin activated Nrf2/ARE pathway as evidenced by the raised Nrf2 and p-Nrf2 levels in nucleus and up-regulation of γ-glutamycysteine synthase(γ-GCS),a well-known target gene of Nrf2/ARE pathway.Nevertheless,pretreatment with a PKC inhibitor(Go 6983)or an Akt inhibitor(MK-22062HCl,reflecting GSK-3β activation)abolished the effect of hesperetin on protein expressions of Glo-1 and γ-GCS.CONCLUSION Hesperetin exerted the neuroprotection by promoting Glo-1 function in central neurons in long-term HG condition,which was mediated by activation of Nrf2/ARE pathway;moreover,the increased Nrf2 phosphorylation and nuclear translocation mediated by PKC activation and/or GSK-3β inhibition were involved in the activation of Nrf2/ARE pathway by hesperetin.
基金Program of Natural Science Foundation of Shanghai,Grant/Award Number:21ZR1453800 and 22ZR1452400Program of National Natural Science Foundation of China,Grant/Award Number:82370057+3 种基金Fundamental Research Funds for the Central Universities,Grant/Award Number:22120220562Program of Shanghai Municipal Health Commission,Grant/Award Number:20204Y0384Program of National Key Research and Development Project of China,Grant/Award Number:2023YFC2509500。
文摘Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.
文摘Today, consumers and producers makes more and more requests for determining the type of honey and its variety because its technological and medicinal qualities depends on the origin. There are many factors which affect on the quality of honey, but the most important is geographical and botanical origin of which largely depends on its medicinal properties. There are several methods of dealing with the classification of honey but the most accurate is pollen analysis with some other tests, such as pH, electrical conductivity and sensory properties. Flavonoids--as one of the most important group of plant secondary metabolites, can be found in honey and its number and amount directly dependents of the origin of honey. In this study, 48 honey samples were tested, of which 29 were pollen honey, 11 were mixed and 8 were honeydew. In all samples, regardless of the type of honey, quercetin and naringenin are found, and rutin and hesperetin are found in-between 36%-50% of honey samples. The largest individual and avarages amounts of tested flavonoids are found in the pollen honey and at least in the mixed honeys.
文摘目的观察丹龙醒脑方对脑缺血再灌注模型大鼠侧脑室室管膜下区(SVZ)神经干细胞(NSCs)增殖与Hes1、Hes5表达的影响,探讨其促进内源性NSCs增殖的作用机制。方法将80只雄性SD大鼠随机分为假手术组、模型组、依达拉奉组(依达组)、丹龙醒脑方组(丹龙组)。采用线栓法制备局灶性脑缺血再灌注模型,再灌注7 d后取缺血侧SVZ脑组织。Brdu免疫荧光法检测SVZ NSCs增殖,RT-q PCR、Western blot分别检测Hes1、Hes5 m RNA和蛋白的表达。结果与假手术组比较,其余各组Brdu阳性细胞率增加,Hes1、Hes5 m RNA及蛋白表达明显升高(P<0.01);与模型组比较,依达组、丹龙组Brdu阳性细胞率明显增加,Hes1、Hes5 m RNA及蛋白表达水平明显增强(P<0.01);丹龙组Hes1 m RNA表达水平优于依达组(P<0.01),其余指标均无明显差异。结论丹龙醒脑方可促进脑缺血再灌注后大鼠SVZ NSCs增殖,并上调Hes1、Hes5表达水平,其机制可能与激活Notch信号通路有关。
