Objective： To observe the immune response after the transplantation of a deproteinized heterogeneous bone scaffold and provides the theoretic reference for clinical practice. Methods： The fresh pig bone and deprote...Objective： To observe the immune response after the transplantation of a deproteinized heterogeneous bone scaffold and provides the theoretic reference for clinical practice. Methods： The fresh pig bone and deproteinized bone were transplanted respectively to establish BABL/C thigh muscle pouches model of male mice and take the samples for detection at 1, 2, 4, 6 weeks after operation. Lymphocyte stimulation index, subset analysis, serum specific antibody IgG, cytokine detection and topographic histologic reaction after implantation were investigated. Results： After the transplantation of deproteinized bone, lymphocyte stimulation index, CD4^＋ and CD8^＋ T-lymphocyte subsets, serum specific antibody IgG and cytokines in deproteinized bone group were significantly lower than those in fresh pig bone group at each time point （P〈0.05）. The histological examination found that in fresh bone group at each time point, a large quantity of inflammatory cells infiltrated in the surrounding of bone graft, and they were mainly lymphocytes, including macrophages and monocytes. In deproteinized bone group, there were few inflammatory cells infiltration around bone graft one week after operation. The lymphocytes were decreased as time went by. At 6 weeks, fibroblasts and fibrous tissue grew into the graft, and osteoclasts and osteoprogenitor cells appeared on the verge. Conclusions： The established heterogeneous deproteinized bone has low immunogenicity and is a potentially ideal scaffold material for bone tissue engineering.展开更多
Objective ： To prepare and observe the physicochemical properties of scaffold materials of heterogeneous deproteinized tissue-engineered bone. Methods： Deproteinized bone was made through a series of physicochemica...Objective ： To prepare and observe the physicochemical properties of scaffold materials of heterogeneous deproteinized tissue-engineered bone. Methods： Deproteinized bone was made through a series of physicochemical treatments in pig ribs and analyzed with histological observation, scanning electron microscopy, infrared spectrum, X-ray diffraction and energy dispersive analysis, Kjeidahl determination and mechanics analysis. Results： Interstitial collagen fiber was positive and mucin was negative in deproteinized bone, but, both were positive in fresh bone. Deproteinized bone maintained natural pore network. Its pore size was 472.51μm ± 7.02μm and the porosity was 78.15 % ± 6.45 %. The results of infrared spectrum showed that collagen was present in deproteinized bone. Both fresh and deproteinized bone had curve of hydroxyapatite. The Ca/P ratios were 1.71 ± 0. 95 and 1.68 ± 0. 76 （ P 〉 0. 05 ）, and the protein contents were26.6% ± 2.23% and 19.1% ± 2.14% （P 〈 0.05） in fresh and deproteinized bone, respectively. There was no significant difference of destruction load under compression and maximal destruction load between fresh and deproteinized bone （P 〉 0. 05）. The elastic modulus was higher in deproteinized bone than that in fresh bone （P 〈 0.05）. Conclusions ： Physicochemical properties and mechanic strength of deproteiulzed tissue-engineered bone meet the demands of ideal scaffold materials. But, its immunogenicity should be observed through further experiments for its clinical applications.展开更多
文摘Objective： To observe the immune response after the transplantation of a deproteinized heterogeneous bone scaffold and provides the theoretic reference for clinical practice. Methods： The fresh pig bone and deproteinized bone were transplanted respectively to establish BABL/C thigh muscle pouches model of male mice and take the samples for detection at 1, 2, 4, 6 weeks after operation. Lymphocyte stimulation index, subset analysis, serum specific antibody IgG, cytokine detection and topographic histologic reaction after implantation were investigated. Results： After the transplantation of deproteinized bone, lymphocyte stimulation index, CD4^＋ and CD8^＋ T-lymphocyte subsets, serum specific antibody IgG and cytokines in deproteinized bone group were significantly lower than those in fresh pig bone group at each time point （P〈0.05）. The histological examination found that in fresh bone group at each time point, a large quantity of inflammatory cells infiltrated in the surrounding of bone graft, and they were mainly lymphocytes, including macrophages and monocytes. In deproteinized bone group, there were few inflammatory cells infiltration around bone graft one week after operation. The lymphocytes were decreased as time went by. At 6 weeks, fibroblasts and fibrous tissue grew into the graft, and osteoclasts and osteoprogenitor cells appeared on the verge. Conclusions： The established heterogeneous deproteinized bone has low immunogenicity and is a potentially ideal scaffold material for bone tissue engineering.
文摘Objective ： To prepare and observe the physicochemical properties of scaffold materials of heterogeneous deproteinized tissue-engineered bone. Methods： Deproteinized bone was made through a series of physicochemical treatments in pig ribs and analyzed with histological observation, scanning electron microscopy, infrared spectrum, X-ray diffraction and energy dispersive analysis, Kjeidahl determination and mechanics analysis. Results： Interstitial collagen fiber was positive and mucin was negative in deproteinized bone, but, both were positive in fresh bone. Deproteinized bone maintained natural pore network. Its pore size was 472.51μm ± 7.02μm and the porosity was 78.15 % ± 6.45 %. The results of infrared spectrum showed that collagen was present in deproteinized bone. Both fresh and deproteinized bone had curve of hydroxyapatite. The Ca/P ratios were 1.71 ± 0. 95 and 1.68 ± 0. 76 （ P 〉 0. 05 ）, and the protein contents were26.6% ± 2.23% and 19.1% ± 2.14% （P 〈 0.05） in fresh and deproteinized bone, respectively. There was no significant difference of destruction load under compression and maximal destruction load between fresh and deproteinized bone （P 〉 0. 05）. The elastic modulus was higher in deproteinized bone than that in fresh bone （P 〈 0.05）. Conclusions ： Physicochemical properties and mechanic strength of deproteiulzed tissue-engineered bone meet the demands of ideal scaffold materials. But, its immunogenicity should be observed through further experiments for its clinical applications.
