Increasing amounts of antibiotic resistant bacteria have been an emergency problem. Antimicrobial peptides are promising antibiotic alternatives for broad-spectrum antimicrobial activity and nearly no drug resistance....Increasing amounts of antibiotic resistant bacteria have been an emergency problem. Antimicrobial peptides are promising antibiotic alternatives for broad-spectrum antimicrobial activity and nearly no drug resistance. Natto peptide was a new antimicrobial peptide which consisted of 45 amino acids. In this study, to improve the antimicrobial activity of Natto peptide, three repeats of encoding sequences were synthesized and cloned into a pET28 a(+) expression vector, and expressed in Escherichia coli as a soluble protein. Unexpectedly, the purified 3×Natto peptide exhibited antimicrobial activity against Listeria monocytogenes(50 μg/ml) and Salmonella enteriditis(30 μg/ml). Furthermore, the antibacterial spectrum of 3×Natto peptide was not affected by temperature, pH value and proteinase digestion. Taken together, this was the first study proving that 3×Natto peptide could be produced in E. coli as a kind of water dissolve protein, and has great potential for commercial application in the future.展开更多
We described a novel single-cell RNA-seq technique called MR-seq （measure a single-cell transcriptome repeatedly）, which permits statistically assessing the technical variation and identifying the differentially exp...We described a novel single-cell RNA-seq technique called MR-seq （measure a single-cell transcriptome repeatedly）, which permits statistically assessing the technical variation and identifying the differentially expressed genes between just two single ceils by measuring each single cell twice. We demonstrated that MR-seq gave sensitivity and reproducibility similar to the standard single-cell RNA-seq and increased the positive predicate value, Application of MR-seq to early mouse embryos identified hundreds of candidate intra-embryonic heterogeneous genes among mouse 2-, 4- and 8-cell stage embryos. MR-seq should be useful for detecting differentially exnre^ed ~enes ~rnnn~ ~ ~m^ll nHmhpr nf c^ll~展开更多
基金Supported by Shandong Provincial Natural Science Foundation(ZR2018PC010)the Doctor Foundation of Binzhou University(2018Y09)Natural Science Program of Binzhou University(BZXYG1811)
文摘Increasing amounts of antibiotic resistant bacteria have been an emergency problem. Antimicrobial peptides are promising antibiotic alternatives for broad-spectrum antimicrobial activity and nearly no drug resistance. Natto peptide was a new antimicrobial peptide which consisted of 45 amino acids. In this study, to improve the antimicrobial activity of Natto peptide, three repeats of encoding sequences were synthesized and cloned into a pET28 a(+) expression vector, and expressed in Escherichia coli as a soluble protein. Unexpectedly, the purified 3×Natto peptide exhibited antimicrobial activity against Listeria monocytogenes(50 μg/ml) and Salmonella enteriditis(30 μg/ml). Furthermore, the antibacterial spectrum of 3×Natto peptide was not affected by temperature, pH value and proteinase digestion. Taken together, this was the first study proving that 3×Natto peptide could be produced in E. coli as a kind of water dissolve protein, and has great potential for commercial application in the future.
基金supported by grants from the Beijing Municipal Science and Technology Commission (D15110700240000)
文摘We described a novel single-cell RNA-seq technique called MR-seq （measure a single-cell transcriptome repeatedly）, which permits statistically assessing the technical variation and identifying the differentially expressed genes between just two single ceils by measuring each single cell twice. We demonstrated that MR-seq gave sensitivity and reproducibility similar to the standard single-cell RNA-seq and increased the positive predicate value, Application of MR-seq to early mouse embryos identified hundreds of candidate intra-embryonic heterogeneous genes among mouse 2-, 4- and 8-cell stage embryos. MR-seq should be useful for detecting differentially exnre^ed ~enes ~rnnn~ ~ ~m^ll nHmhpr nf c^ll~
