Arisaema heterophyllum Blume is one of the three medicinal plants known as traditional Chinese medicine Rhizoma Arisaematis(RA). RA has been popularly used to treat patients with convulsions, inflammation, and cancer ...Arisaema heterophyllum Blume is one of the three medicinal plants known as traditional Chinese medicine Rhizoma Arisaematis(RA). RA has been popularly used to treat patients with convulsions, inflammation, and cancer for a long time. However, the underlying mechanisms for RA effects are still unclear. The present study was designed to determine the cytotoxicity of agglutinin isolated from Arisema heterophyllum Blume(AHA) and explore the possible mechanisms in human non-small-cell lung cancer A549 cells. AHA with purity up to 95% was isolated and purified from Arisaema heterophyllum Blume using hydrophobic interaction chromatography. AHA dose-dependently inhibited the proliferation of A549 cells and induced G_1 phase cell cycle arrest. AHA induced apoptosis by up-regulating pro-apoptotic Bax, decreasing anti-apoptotic Bcl-2, and activating caspase-9 and caspase-3. In A549 cells treated with AHA, the PI3K/Akt pathway was inhibited. Furthermore, AHA induced increase in the levels of ER stress markers such as phosphorylated eukaryotic initiation factor 2α(p-eIF2α), C/EBP-homologous protein(CHOP), inositol-requiring enzyme 1α(IRE1α), and phosphorylated c-Jun NH_2-terminal kinase(p-JNK). AHA also induced autophagy in A549 cells. Staining of acidic vesicular organelles(AVOs) and increase in the levels of LC3II and ATG7 were observed in AHA-treated cells. These findings suggested that AHA might be one of the active components with anti-cancer effects in Arisaema heterophyllum Blume. In conclusion, cytotoxicity of AHA on cancer cells might be related to its effects on apoptosis and autophagy through inhibition of PI3K/Akt pathway and induction of ER stress.展开更多
Aconitum heterophyllum(Patrees)is a critically endangered medicinal herb of the northwestern Himalayas and has enormous pharmacological potential.It is the only nonpoisonous member of the genus Aconitum,and has been u...Aconitum heterophyllum(Patrees)is a critically endangered medicinal herb of the northwestern Himalayas and has enormous pharmacological potential.It is the only nonpoisonous member of the genus Aconitum,and has been used as a medicinal herb since ancient times.A.heterophyllum is an important ingredient in many traditional systems of medicine.Mostly,it is harvested for its roots,and its medicinal properties are due to the presence of diverse bioactive secondary metabolites,commonly known as aconites.Our understanding of the pharmacological properties of this intriguing genus is continuously growing due to its broad chemical diversity.The therapeutic uses identified by traditional medicinal practice are receiving extensive study.Multiple in vitro experimental investigations of A.heterophyllum have reported the analgesic,anti-inflammatory,antiarrhythmic,antiparasitic and anticancer properties,as well as its effects on the central nervous system.In this review,we highlight the classification,distribution,commerce,traditional uses,phytochemistry,pharmacology and conservation measures relevant to this species.Additionally,this review includes the biosynthetic pathways of A.heterophyllum’s key constituents,which could be targeted to enhance the expression levels of desired metabolites via genetic interventions.Studying the genomics,transcriptomics,proteomics and metabolomic aspects of this species would be helpful in developing highly designed genotypes and chemotypes of this species to be used in commercial production.展开更多
Aconitum heterophyllum is a plant that has very important medicinal value.Lots of phytochemical constituents(metabolites)are extracted from this plant,especially diterpene alkaloids which are the main compounds having...Aconitum heterophyllum is a plant that has very important medicinal value.Lots of phytochemical constituents(metabolites)are extracted from this plant,especially diterpene alkaloids which are the main compounds having pharmacological activities such as analgesic and anti-inflammatory.Study of the structures of these compounds was done by the technique of nuclear magnetic resonance.展开更多
基金supported by the National Nature Science Foundation of China(No.81373964)Shanghai Science&Technology Support Program(No.13431900401)+1 种基金Shanghai Science&Technology Innovation Action Program(No.15140904800)the National Science&Technology Major Project of China(No.2014 ZX09301-306-03)
文摘Arisaema heterophyllum Blume is one of the three medicinal plants known as traditional Chinese medicine Rhizoma Arisaematis(RA). RA has been popularly used to treat patients with convulsions, inflammation, and cancer for a long time. However, the underlying mechanisms for RA effects are still unclear. The present study was designed to determine the cytotoxicity of agglutinin isolated from Arisema heterophyllum Blume(AHA) and explore the possible mechanisms in human non-small-cell lung cancer A549 cells. AHA with purity up to 95% was isolated and purified from Arisaema heterophyllum Blume using hydrophobic interaction chromatography. AHA dose-dependently inhibited the proliferation of A549 cells and induced G_1 phase cell cycle arrest. AHA induced apoptosis by up-regulating pro-apoptotic Bax, decreasing anti-apoptotic Bcl-2, and activating caspase-9 and caspase-3. In A549 cells treated with AHA, the PI3K/Akt pathway was inhibited. Furthermore, AHA induced increase in the levels of ER stress markers such as phosphorylated eukaryotic initiation factor 2α(p-eIF2α), C/EBP-homologous protein(CHOP), inositol-requiring enzyme 1α(IRE1α), and phosphorylated c-Jun NH_2-terminal kinase(p-JNK). AHA also induced autophagy in A549 cells. Staining of acidic vesicular organelles(AVOs) and increase in the levels of LC3II and ATG7 were observed in AHA-treated cells. These findings suggested that AHA might be one of the active components with anti-cancer effects in Arisaema heterophyllum Blume. In conclusion, cytotoxicity of AHA on cancer cells might be related to its effects on apoptosis and autophagy through inhibition of PI3K/Akt pathway and induction of ER stress.
基金supported by Department of Science and Technology under SERB-NPDF schemeDST-SERB for providing National Postdoc Fellowship under file No.PDF/2016/002997/LS+1 种基金DST-SERB for providing National Postdoc Fellowship under file No.PDF/2017/002716CSIR for providing SRA(Scientist Pool)fellowship under pool number 9092-A。
文摘Aconitum heterophyllum(Patrees)is a critically endangered medicinal herb of the northwestern Himalayas and has enormous pharmacological potential.It is the only nonpoisonous member of the genus Aconitum,and has been used as a medicinal herb since ancient times.A.heterophyllum is an important ingredient in many traditional systems of medicine.Mostly,it is harvested for its roots,and its medicinal properties are due to the presence of diverse bioactive secondary metabolites,commonly known as aconites.Our understanding of the pharmacological properties of this intriguing genus is continuously growing due to its broad chemical diversity.The therapeutic uses identified by traditional medicinal practice are receiving extensive study.Multiple in vitro experimental investigations of A.heterophyllum have reported the analgesic,anti-inflammatory,antiarrhythmic,antiparasitic and anticancer properties,as well as its effects on the central nervous system.In this review,we highlight the classification,distribution,commerce,traditional uses,phytochemistry,pharmacology and conservation measures relevant to this species.Additionally,this review includes the biosynthetic pathways of A.heterophyllum’s key constituents,which could be targeted to enhance the expression levels of desired metabolites via genetic interventions.Studying the genomics,transcriptomics,proteomics and metabolomic aspects of this species would be helpful in developing highly designed genotypes and chemotypes of this species to be used in commercial production.
文摘Aconitum heterophyllum is a plant that has very important medicinal value.Lots of phytochemical constituents(metabolites)are extracted from this plant,especially diterpene alkaloids which are the main compounds having pharmacological activities such as analgesic and anti-inflammatory.Study of the structures of these compounds was done by the technique of nuclear magnetic resonance.