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Ni^(2+)-PAA Adsorbent for Purifying 6His-OmpTS Recombinant Protein
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作者 QingBingZENG QiaoZhenYE 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第5期436-439,共4页
Functional Ni2+-polyacrylic acid (Ni2+-PAA) adsorbent has been prepared for metal chelate affinity chromatography. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subse... Functional Ni2+-polyacrylic acid (Ni2+-PAA) adsorbent has been prepared for metal chelate affinity chromatography. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subsequent expression in E. coli resulted in the production of hybrid protein 6His-OmpTS that could be purified by Ni2+-PAA affinity chromatography. 展开更多
关键词 Affinity matrix polyacrylic acid CHELATE nickel ion hexa-histidine tagged.
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