A new CoII complex, [CoL4C12].2CH3CH2OH（L=hexaconazole）, was synthesized and characterized by means of elemental analysis, infrared spectroscopy and single-crystal X-ray diffraction. The crystal structural analysis ...A new CoII complex, [CoL4C12].2CH3CH2OH（L=hexaconazole）, was synthesized and characterized by means of elemental analysis, infrared spectroscopy and single-crystal X-ray diffraction. The crystal structural analysis shows that the centrosymmetric Co2＋ is coordinated by four hexaconazole ligands and two C1 to form a distorted octahedral geometry. The complex forms 1D chains through the intermolecular hydrogen bonds interaction of O-H-… O and O--H…… C1. Furthermore, the antifungal activities of the complex against Grape anthracnose（I）, Botryosphaeria berengriana（II）, Botryosphaeria ribis（III） and Wheat gibberellic（IV） have been investigated and compared with those of the corresponding ligand. The results show that the complex has better antifungal activity than the ligand, which indicates that the coordination of the ligand and the metal enhances the antimicrobialactivity. Keywords展开更多
Bacterial strain RV9 recovered from greengram nodules tolerated 2400 μg/mL of hexaconazole and was identified by 16 S rDNA sequence analysis as Bradyrhizobium japonicum(KY940048). Strain RV9 produced IAA(61.6 μg/mL)...Bacterial strain RV9 recovered from greengram nodules tolerated 2400 μg/mL of hexaconazole and was identified by 16 S rDNA sequence analysis as Bradyrhizobium japonicum(KY940048). Strain RV9 produced IAA(61.6 μg/mL), ACC deaminase(51.7 mg/(protein·hr)), solubilized TCP(105 μg/mL), secreted 337.6 μg/mL EPS, and produced SA(52.2 μg/mL) and 2,3-DHBA(28.3 μg/mL). Exopolysaccharides produced by strain RV9 was quantified and characterized by SEM, AFM, EDX and FTIR. Beyond tolerance limit,hexaconazole caused cellular impairment and reduced the viability of strain RV9 revealed by SEM and CLSM. Hexaconazole distorted the root tips and altered nodule structure leading thereby to reduction in the performance of greengram. Also, the level of antioxidant enzymes, proline, TBARS, ROS and cell death was increased in hexaconazole treated plants.CLSM images revealed a concentration dependent increase in the characteristic green and blue fluorescence of hexaconazole treated roots. The application of B. japonicum strain RV9 alleviated the fungicide toxicity and improved the measured plant characteristics. Also,rhizobial cells were localized inside tissues as revealed by CLSM. Colonization of B.japonicum strain RV9 decreased the levels of CAT, POD, APX, GPX and TBARS by 80%, 5%,13%, 13% and 19%, respectively over plants grown at 80 μg/(hexaconazole·kg) soil. The ability to detoxify hexaconazole, colonize plant tissues, secrete PGP bioactive molecules even under fungicide pressure and its unique ability to diminish oxidative stress make B.japonicum an attractive choice for remediation of fungicide polluted soils and to concurrently enhance greengram production under stressed environment.展开更多
基金Supported by the Provincial Key-point Natural Science Foundation of Shaanxi, China（No.2016JZ003） and the Training Pro- gram of Innovation and Entrepreneurship for Undergraduates in Northwest University, China（No. 2016225）.
文摘A new CoII complex, [CoL4C12].2CH3CH2OH（L=hexaconazole）, was synthesized and characterized by means of elemental analysis, infrared spectroscopy and single-crystal X-ray diffraction. The crystal structural analysis shows that the centrosymmetric Co2＋ is coordinated by four hexaconazole ligands and two C1 to form a distorted octahedral geometry. The complex forms 1D chains through the intermolecular hydrogen bonds interaction of O-H-… O and O--H…… C1. Furthermore, the antifungal activities of the complex against Grape anthracnose（I）, Botryosphaeria berengriana（II）, Botryosphaeria ribis（III） and Wheat gibberellic（IV） have been investigated and compared with those of the corresponding ligand. The results show that the complex has better antifungal activity than the ligand, which indicates that the coordination of the ligand and the metal enhances the antimicrobialactivity. Keywords
基金the financial support received in the form of UGC NonNET fellowship granted by University Grants Commission (D. O.No.F.1993/2006 (CU) dated 01.02.2007), New Delhi
文摘Bacterial strain RV9 recovered from greengram nodules tolerated 2400 μg/mL of hexaconazole and was identified by 16 S rDNA sequence analysis as Bradyrhizobium japonicum(KY940048). Strain RV9 produced IAA(61.6 μg/mL), ACC deaminase(51.7 mg/(protein·hr)), solubilized TCP(105 μg/mL), secreted 337.6 μg/mL EPS, and produced SA(52.2 μg/mL) and 2,3-DHBA(28.3 μg/mL). Exopolysaccharides produced by strain RV9 was quantified and characterized by SEM, AFM, EDX and FTIR. Beyond tolerance limit,hexaconazole caused cellular impairment and reduced the viability of strain RV9 revealed by SEM and CLSM. Hexaconazole distorted the root tips and altered nodule structure leading thereby to reduction in the performance of greengram. Also, the level of antioxidant enzymes, proline, TBARS, ROS and cell death was increased in hexaconazole treated plants.CLSM images revealed a concentration dependent increase in the characteristic green and blue fluorescence of hexaconazole treated roots. The application of B. japonicum strain RV9 alleviated the fungicide toxicity and improved the measured plant characteristics. Also,rhizobial cells were localized inside tissues as revealed by CLSM. Colonization of B.japonicum strain RV9 decreased the levels of CAT, POD, APX, GPX and TBARS by 80%, 5%,13%, 13% and 19%, respectively over plants grown at 80 μg/(hexaconazole·kg) soil. The ability to detoxify hexaconazole, colonize plant tissues, secrete PGP bioactive molecules even under fungicide pressure and its unique ability to diminish oxidative stress make B.japonicum an attractive choice for remediation of fungicide polluted soils and to concurrently enhance greengram production under stressed environment.
