AIM:To evaluate effects of endogenous dopamine induced by low concentration atropine eye drops on choroidal neovascularization(CNV)in high myopia mice.METHODS:The C57BL/6J mice were deprived of the right eye for 4wk,a...AIM:To evaluate effects of endogenous dopamine induced by low concentration atropine eye drops on choroidal neovascularization(CNV)in high myopia mice.METHODS:The C57BL/6J mice were deprived of the right eye for 4wk,and the high myopia was diagnosed by optometry,the diopter was less than-6.00 D,and CNV was induced by 532 nm laser.The changes of dopamine D1 receptor(DRD1),dopamine D2 receptor(DRD2),and vascular endothelial growth factor A(VEGFA)were detected by Western blot technology at 0.5,1,2h,and 7d after 0.01%,0.05%,and 0.1%atropine eye drops,respectively,the area of CNV was measured.RESULTS:Significant increases were observed on the expression of DRD2 in mouse high myopia model at 0.5,1,2h,7d with 0.05%and 0.1%atropine eye drops(P<0.05).Significant decreases were observed on the expression of DRD1 and VEGFA in mouse high myopia model at 0.5,1,2h,7d with 0.05%and 0.1%atropine eye drops(P<0.05).The area of CNV induced by laser in the drug-treated group was significantly smaller than that in the control group,and the higher the concentration,the more significant the inhibitory effect(P<0.05).CONCLUSION:The 0.01%,0.05%,0.1%atropine eye drops can decrease the level of VEGFA and inhibit high myopia CNV indirectly by up-regulating the level of DRD2 and down-regulating the level of DRD1,and the effect of 0.05%and 0.1%atropine eye drops is more significant.展开更多
To analyze the relation of matrix metalloproteinase-2(MMP-2) and Fibronection (FN) mRNA expression with metastasis of breast cancer and elucidate the role of MMP-2 and FN in breast cancer metastasis.Methods The expres...To analyze the relation of matrix metalloproteinase-2(MMP-2) and Fibronection (FN) mRNA expression with metastasis of breast cancer and elucidate the role of MMP-2 and FN in breast cancer metastasis.Methods The expression of MMP-2 and FN mRNA in breast cancer cell lines was detected by fluorescence-quantitative RT-PCR.The expression of MMP-2 and FN protein was detected by Western blots.Results The expression of MMP-2 and FN mRNA was down-regulated in high metastatic cell lines MDA-MB-231,MDA-MB-435,but up-regulated in low metastatic cell lines MDA-453,T47D,SK-BR-3 and non-metastatic cell line MCF-7,ZR-75-30.The protein expression of MMP-2 and FN was up-regulated in high mestastic cell lines,and down-regulated in low metastatic cell lines.Conclusion The mRNA and protein expression of MMP-2 and FN was related with breast cancer metastasis.The mRNA expression of MMP-2 and FN is feed-back regulated with protein expression.6 refs,4 figs,2 tabs.展开更多
Localized CdS homojunctions with optimal ratio of high and low index facets are constructed to dy-namically boost H_(2)O splitting into H_(2)energy by hydrothermal method in combination with calcination.By density fun...Localized CdS homojunctions with optimal ratio of high and low index facets are constructed to dy-namically boost H_(2)O splitting into H_(2)energy by hydrothermal method in combination with calcination.By density functional theory,hall effect,and in situ diffuse reflectance infrared Fourier transform spec-troscopy,it is revealed that photo-irradiated e^(−)and h^(+)can be spatially separated and directionally trans-ferred to the reductive high-index facet{002}and oxidative low-index facet{110}of localized CdS homo-junction induced by Fermi level difference of both high and low index facets to dehydrogenate ^(∗)-OH and coupled ^(∗)-O intermediates for H_(2)and O_(2)yield,respectively,along with a solar conversion into hydrogen of 1.93%by AM 1.5 G irradiation at 65℃.The study work suggests a scientific perspective on the optimal ratio of high and low index facets to understand photo-generated charge carrier transfer dynamically and their photocatalytic principle for H_(2)O splitting reaction in kinetics.展开更多
Lithium metal has gained extensive attention as the most ideal candidate for next-generation battery anode owing to the ultrahigh specific capacity and the lowest electrochemical potential.However,uncontrollable dendr...Lithium metal has gained extensive attention as the most ideal candidate for next-generation battery anode owing to the ultrahigh specific capacity and the lowest electrochemical potential.However,uncontrollable dendrite growth and huge volume variation extremely restrict the future deployment of lithium metal batteries.Herein,we report metal chalcogenide SnSSe with unique nanoplate stacking structure as a robust substrate for stable Li metal anode.During the initial Li plating process,lithiophilic Li_(22)Sn_(5) alloy and Li_(2)S/Li_(2)Se sites are obtained via in-situ electrochemical reaction of Li metal and SnSSe.Density functional theory(DFT)calculation demonstrates that the formed Li_(2)S/Li_(2)Se achieves low Li diffusion energy barrier,ensuring rapid Li~+migration.Li_(22)Sn_(5) alloy provides strong nucleation sites,promoting uniform Li nucleation.Furthermore,in-situ optical microscopy analysis suggests that the synthesized effect fundamentally inhibits lithium dendrite growth.Consequently,SnSSe modified Cu foil delivered an ultralow nucleation overpotential,superior cycling stability with 450 cycles(Coulombic efficiency,>98%),and excellent plating/stripping behavior over 2200 h at 0.5 mA cm^(-2).Moreover,the brilliant reversible cycles and rate capability were also realized in Li@SnSSe//LiFePO_(4)(LFP)full cell,shedding light on the feasibility of SnSSe for stable and dendrite-free lithium metal anode.展开更多
CsPbI_(2)Br perovskite solar cells have achieved rapid development owing to their exceptional optoelectronic properties and relatively outstanding stability.However,open-circuit voltage(Voc)loss caused by band mismatc...CsPbI_(2)Br perovskite solar cells have achieved rapid development owing to their exceptional optoelectronic properties and relatively outstanding stability.However,open-circuit voltage(Voc)loss caused by band mismatch and charge recombination between perovskite and charge transporting layer is one of the crucial obstacles to further improve the device performance.Here,we proposed a bilayer electron transport layer ZnO(bottom)/SnO_(2)(top)to reduce the Voc loss(Eloss)and promote device Voc by ZnO insert layer thickness modulation,which could improve the efficiency of charge carrier extraction/transfer and suppress the charge carrier recombination.In addition,guanidinium iodide top surface treatment is used to further reduce the trap density,stabilize the perovskite film and align the energy levels,which promotes the fill factor,short-circuit current density(Jsc),and stability of the device.As a result,the champion cell of double-side optimized CsPbI_(2)Br perovskite solar cells exhibits an extraordinary efficiency of 16.25%with the best Voc as high as 1.27 V and excellent thermal and storage stability.展开更多
In order to analyze the influence of the addition of yttrium and manganese on the soot combustion performance and high temperature stability of CeO_(2) catalyst,a series of Y/Mn-modified CeO_(2) catalysts were prepare...In order to analyze the influence of the addition of yttrium and manganese on the soot combustion performance and high temperature stability of CeO_(2) catalyst,a series of Y/Mn-modified CeO_(2) catalysts were prepared.The effects of structural properties,textural properties,oxygen vacancies,Ce^(3+),surface adsorbed oxygen species,reduction properties and desorption properties of oxygen species on the activity were analyzed by various characterization methods.The results of the activity test show that the addition of manganese is beneficial to enhancement of the activity,while the addition of yttrium increases the amount of reactive oxygen species,but decreases the activity.After aging at 700℃,the activity of the CeMn catalyst decreases most sharply,while the catalytic activity of the CeY catalyst can be maintained to a certain extent.Interestingly,the addition of yttrium and manganese at the same time can stabilize the activity.The fundamental reason is that yttrium and manganese move to the surface of the solid solution after aging,which increases the reduction performance of the catalyst,thus contributing to the increase of activity.Although the activity of CeYMn catalyst decreases after aging at 800℃,it is still higher than that of other catalysts aged at 700℃.展开更多
以200 m Pa·s和500 m Pa·s的端乙烯基硅油为基体硅油、活性氢质量分数0.05%含氢硅油为交联剂,添加不同粒径球形氧化铝以不同比例复配的导热填料制得低渗油高填充导热硅凝胶。研究了不同粒径球形氧化铝与基体硅油黏度对基胶黏...以200 m Pa·s和500 m Pa·s的端乙烯基硅油为基体硅油、活性氢质量分数0.05%含氢硅油为交联剂,添加不同粒径球形氧化铝以不同比例复配的导热填料制得低渗油高填充导热硅凝胶。研究了不同粒径球形氧化铝与基体硅油黏度对基胶黏度及氧化铝最大填充量的影响,探究了γ-巯丙基三甲氧基硅烷(KH590)对硅凝胶导热性能的影响,基体硅油黏度、活性氢与硅乙烯基物质的量之比及氧化铝填充量对硅凝胶硬度、密度、热导率及力学强度的影响。重点研究了氧化铝复配种类与比例对硅凝胶抗渗油性能及热稳定性的影响机制。结果表明,以不同黏度的端乙烯基硅油为基体硅油,活性氢质量分数0.05%含氢硅油为交联剂,添加不同粒径的球形氧化铝复配导热填料可制得低渗油高填充导热硅凝胶;在采用单一粒径球形氧化铝时,同一填充量下,20μm球形氧化铝对基胶的增黏不明显且最大填充量较高,可达1 500份;适当增加硅烷偶联剂KH590用量能有效提高硅凝胶的导热性能,较佳用量为2.00%;采用200 m Pa·s的端乙烯基硅油为基体硅油,活性氢与硅乙烯基物质的量之比为0.8∶1,单独采用600份20μm球形氧化铝作导热填料时,硅凝胶具有较佳的硬度、密度、热导率及拉伸强度,邵氏OO硬度为67,密度为1.83 g/cm^(3),热导率为3.69 W/(m·K),拉伸强度为1.95 MPa;采用不同粒径球形氧化铝复配时,导热硅凝胶的热导率均高于采用单一粒径球形氧化铝填充的硅凝胶,导热填料采用20μm与5μm球形氧化铝按质量比2∶1复配时硅凝胶导热性能较佳,热导率为4.72 W/(m·K);由于球形氧化铝表面硅烷化后与基体硅油的缠绕及填料表面巯基与一定量残留双键的氢键效应,导热填料采用20μm与5μm球形氧化铝按质量比2∶1复配时硅凝胶具有较佳的抗渗油性能及热稳定性,渗油比仅为1.0476,硅凝胶质量损失率5%时的温度为331℃,700℃时残余质量分数为89.10%。展开更多
文摘AIM:To evaluate effects of endogenous dopamine induced by low concentration atropine eye drops on choroidal neovascularization(CNV)in high myopia mice.METHODS:The C57BL/6J mice were deprived of the right eye for 4wk,and the high myopia was diagnosed by optometry,the diopter was less than-6.00 D,and CNV was induced by 532 nm laser.The changes of dopamine D1 receptor(DRD1),dopamine D2 receptor(DRD2),and vascular endothelial growth factor A(VEGFA)were detected by Western blot technology at 0.5,1,2h,and 7d after 0.01%,0.05%,and 0.1%atropine eye drops,respectively,the area of CNV was measured.RESULTS:Significant increases were observed on the expression of DRD2 in mouse high myopia model at 0.5,1,2h,7d with 0.05%and 0.1%atropine eye drops(P<0.05).Significant decreases were observed on the expression of DRD1 and VEGFA in mouse high myopia model at 0.5,1,2h,7d with 0.05%and 0.1%atropine eye drops(P<0.05).The area of CNV induced by laser in the drug-treated group was significantly smaller than that in the control group,and the higher the concentration,the more significant the inhibitory effect(P<0.05).CONCLUSION:The 0.01%,0.05%,0.1%atropine eye drops can decrease the level of VEGFA and inhibit high myopia CNV indirectly by up-regulating the level of DRD2 and down-regulating the level of DRD1,and the effect of 0.05%and 0.1%atropine eye drops is more significant.
文摘To analyze the relation of matrix metalloproteinase-2(MMP-2) and Fibronection (FN) mRNA expression with metastasis of breast cancer and elucidate the role of MMP-2 and FN in breast cancer metastasis.Methods The expression of MMP-2 and FN mRNA in breast cancer cell lines was detected by fluorescence-quantitative RT-PCR.The expression of MMP-2 and FN protein was detected by Western blots.Results The expression of MMP-2 and FN mRNA was down-regulated in high metastatic cell lines MDA-MB-231,MDA-MB-435,but up-regulated in low metastatic cell lines MDA-453,T47D,SK-BR-3 and non-metastatic cell line MCF-7,ZR-75-30.The protein expression of MMP-2 and FN was up-regulated in high mestastic cell lines,and down-regulated in low metastatic cell lines.Conclusion The mRNA and protein expression of MMP-2 and FN was related with breast cancer metastasis.The mRNA expression of MMP-2 and FN is feed-back regulated with protein expression.6 refs,4 figs,2 tabs.
基金supported by the National Natural Science Foundation of China(No.51972177)the Natural Science Foundation of Ningbo City(No.2021J067)the SJLY2021010 of Ningbo University,Fan 3315 Plan,and Yongjiang Scholar Project.
文摘Localized CdS homojunctions with optimal ratio of high and low index facets are constructed to dy-namically boost H_(2)O splitting into H_(2)energy by hydrothermal method in combination with calcination.By density functional theory,hall effect,and in situ diffuse reflectance infrared Fourier transform spec-troscopy,it is revealed that photo-irradiated e^(−)and h^(+)can be spatially separated and directionally trans-ferred to the reductive high-index facet{002}and oxidative low-index facet{110}of localized CdS homo-junction induced by Fermi level difference of both high and low index facets to dehydrogenate ^(∗)-OH and coupled ^(∗)-O intermediates for H_(2)and O_(2)yield,respectively,along with a solar conversion into hydrogen of 1.93%by AM 1.5 G irradiation at 65℃.The study work suggests a scientific perspective on the optimal ratio of high and low index facets to understand photo-generated charge carrier transfer dynamically and their photocatalytic principle for H_(2)O splitting reaction in kinetics.
基金financially supported by the National Natural Science Foundation of China(52074113,22005091,and 22005092)the Hunan University Outstanding Youth Science Foundation(531118040319)+5 种基金the Science and Technology Innovation Program of Hunan Province(2021RC3055)the Changsha Municipal Natural Science Foundation(kq2014037)the CITIC Metals Ningbo Energy Co.Ltd.(H202191380246)the Chongqing Talents:Exceptional Young Talents Project(CQYC202105015)the Shenzhen Virtual University Park Basic Research Project of Free exploration(2021Szvup036)financially supported by the Graduate Research and Innovation Projects of Hunan Province(QL20210088)。
文摘Lithium metal has gained extensive attention as the most ideal candidate for next-generation battery anode owing to the ultrahigh specific capacity and the lowest electrochemical potential.However,uncontrollable dendrite growth and huge volume variation extremely restrict the future deployment of lithium metal batteries.Herein,we report metal chalcogenide SnSSe with unique nanoplate stacking structure as a robust substrate for stable Li metal anode.During the initial Li plating process,lithiophilic Li_(22)Sn_(5) alloy and Li_(2)S/Li_(2)Se sites are obtained via in-situ electrochemical reaction of Li metal and SnSSe.Density functional theory(DFT)calculation demonstrates that the formed Li_(2)S/Li_(2)Se achieves low Li diffusion energy barrier,ensuring rapid Li~+migration.Li_(22)Sn_(5) alloy provides strong nucleation sites,promoting uniform Li nucleation.Furthermore,in-situ optical microscopy analysis suggests that the synthesized effect fundamentally inhibits lithium dendrite growth.Consequently,SnSSe modified Cu foil delivered an ultralow nucleation overpotential,superior cycling stability with 450 cycles(Coulombic efficiency,>98%),and excellent plating/stripping behavior over 2200 h at 0.5 mA cm^(-2).Moreover,the brilliant reversible cycles and rate capability were also realized in Li@SnSSe//LiFePO_(4)(LFP)full cell,shedding light on the feasibility of SnSSe for stable and dendrite-free lithium metal anode.
基金supported by National Natural Science Foundation of China(61704131 and 61804111)National Key Research and Development Program of China(Grant 2018YFB2202900)+2 种基金Key Research and Development Program of Shaanxi Province(Grant 2020GY-310)the Joint Research Funds of Department of Science&Technology of Shaanxi Province and Northwestern Polytechnical University(2020GXLH-Z-018)the Fundamental Research Funds for the Central Universities and the Innovation Fund of Xidian University.
文摘CsPbI_(2)Br perovskite solar cells have achieved rapid development owing to their exceptional optoelectronic properties and relatively outstanding stability.However,open-circuit voltage(Voc)loss caused by band mismatch and charge recombination between perovskite and charge transporting layer is one of the crucial obstacles to further improve the device performance.Here,we proposed a bilayer electron transport layer ZnO(bottom)/SnO_(2)(top)to reduce the Voc loss(Eloss)and promote device Voc by ZnO insert layer thickness modulation,which could improve the efficiency of charge carrier extraction/transfer and suppress the charge carrier recombination.In addition,guanidinium iodide top surface treatment is used to further reduce the trap density,stabilize the perovskite film and align the energy levels,which promotes the fill factor,short-circuit current density(Jsc),and stability of the device.As a result,the champion cell of double-side optimized CsPbI_(2)Br perovskite solar cells exhibits an extraordinary efficiency of 16.25%with the best Voc as high as 1.27 V and excellent thermal and storage stability.
基金Project supported by the National Natural Science Foundation of China(21962021)the Yunnan Fundamental Research Projects(202001AU070121)+1 种基金the National Natural Science Foundation of China(51908091)the Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities'Association(202101BA070001-084)。
文摘In order to analyze the influence of the addition of yttrium and manganese on the soot combustion performance and high temperature stability of CeO_(2) catalyst,a series of Y/Mn-modified CeO_(2) catalysts were prepared.The effects of structural properties,textural properties,oxygen vacancies,Ce^(3+),surface adsorbed oxygen species,reduction properties and desorption properties of oxygen species on the activity were analyzed by various characterization methods.The results of the activity test show that the addition of manganese is beneficial to enhancement of the activity,while the addition of yttrium increases the amount of reactive oxygen species,but decreases the activity.After aging at 700℃,the activity of the CeMn catalyst decreases most sharply,while the catalytic activity of the CeY catalyst can be maintained to a certain extent.Interestingly,the addition of yttrium and manganese at the same time can stabilize the activity.The fundamental reason is that yttrium and manganese move to the surface of the solid solution after aging,which increases the reduction performance of the catalyst,thus contributing to the increase of activity.Although the activity of CeYMn catalyst decreases after aging at 800℃,it is still higher than that of other catalysts aged at 700℃.
文摘以200 m Pa·s和500 m Pa·s的端乙烯基硅油为基体硅油、活性氢质量分数0.05%含氢硅油为交联剂,添加不同粒径球形氧化铝以不同比例复配的导热填料制得低渗油高填充导热硅凝胶。研究了不同粒径球形氧化铝与基体硅油黏度对基胶黏度及氧化铝最大填充量的影响,探究了γ-巯丙基三甲氧基硅烷(KH590)对硅凝胶导热性能的影响,基体硅油黏度、活性氢与硅乙烯基物质的量之比及氧化铝填充量对硅凝胶硬度、密度、热导率及力学强度的影响。重点研究了氧化铝复配种类与比例对硅凝胶抗渗油性能及热稳定性的影响机制。结果表明,以不同黏度的端乙烯基硅油为基体硅油,活性氢质量分数0.05%含氢硅油为交联剂,添加不同粒径的球形氧化铝复配导热填料可制得低渗油高填充导热硅凝胶;在采用单一粒径球形氧化铝时,同一填充量下,20μm球形氧化铝对基胶的增黏不明显且最大填充量较高,可达1 500份;适当增加硅烷偶联剂KH590用量能有效提高硅凝胶的导热性能,较佳用量为2.00%;采用200 m Pa·s的端乙烯基硅油为基体硅油,活性氢与硅乙烯基物质的量之比为0.8∶1,单独采用600份20μm球形氧化铝作导热填料时,硅凝胶具有较佳的硬度、密度、热导率及拉伸强度,邵氏OO硬度为67,密度为1.83 g/cm^(3),热导率为3.69 W/(m·K),拉伸强度为1.95 MPa;采用不同粒径球形氧化铝复配时,导热硅凝胶的热导率均高于采用单一粒径球形氧化铝填充的硅凝胶,导热填料采用20μm与5μm球形氧化铝按质量比2∶1复配时硅凝胶导热性能较佳,热导率为4.72 W/(m·K);由于球形氧化铝表面硅烷化后与基体硅油的缠绕及填料表面巯基与一定量残留双键的氢键效应,导热填料采用20μm与5μm球形氧化铝按质量比2∶1复配时硅凝胶具有较佳的抗渗油性能及热稳定性,渗油比仅为1.0476,硅凝胶质量损失率5%时的温度为331℃,700℃时残余质量分数为89.10%。
